{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T17:15:50Z","timestamp":1790615750029,"version":"4.1.0"},"reference-count":42,"publisher":"MDPI AG","issue":"19","license":[{"start":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T00:00:00Z","timestamp":1790553600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82360683"],"award-info":[{"award-number":["82360683"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82360805"],"award-info":[{"award-number":["82360805"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"award":["82360683"],"award-info":[{"award-number":["82360683"]}],"id":[{"id":"https:\/\/ror.org\/01h0zpd94","id-type":"ROR","asserted-by":"publisher"}]},{"award":["82360805"],"award-info":[{"award-number":["82360805"]}],"id":[{"id":"https:\/\/ror.org\/01h0zpd94","id-type":"ROR","asserted-by":"publisher"}]},{"name":"Jilin Provincial Natural Science Foundation Project","award":["YDZJ202601ZYTS280"],"award-info":[{"award-number":["YDZJ202601ZYTS280"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Foods"],"abstract":"<jats:p>Three polysaccharide fractions (SH-40, SH-60, and SH-80) were sequentially separated from Sanghuangporus vaninii fruiting bodies through graded ethanol precipitation to investigate the influence of molecular weight on their structure, physicochemical properties, and inhibition of tumor cell proliferation. The results revealed that the molecular weights of these three fractions were approximately 5.90 \u00d7 105, 4.82 \u00d7 105, and 4.13 \u00d7 105 Da, respectively; they shared similar functional group profiles, monosaccharide compositions and glycosidic bonds. As the molecular weight decreased, the water holding capacity (WHC) declined, while the water solubility index (WSI) and oil holding capacity (OHC) increased. In tumor cell proliferation inhibition assays, all three fractions inhibited the proliferation of MCF-7, HepG2, A549, and SGC-7901 cells in a concentration-dependent manner and maintained a high cell survival rate (&gt;70%) in normal HUVECs; among them, SH-40 showed the lowest half-maximal inhibitory concentration (IC50) value (1.09\u20133.89 mg\/mL) (p &lt; 0.05). SH-40 displayed the highest biological activity due to its higher molecular weight and characteristic monosaccharide ratio (p &lt; 0.05), suggesting that molecular weight was one of the important factors influencing its function and activity. These findings provided a basis for the hierarchical preparation and differentiated application of polysaccharides isolated from Sanghuangporus vaninii.<\/jats:p>","DOI":"10.3390\/foods15193474","type":"journal-article","created":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T15:57:57Z","timestamp":1790611077000},"page":"3474","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Gradient Ethanol Precipitation of Sanghuangporus vaninii Polysaccharides: Physicochemical Properties and Inhibition of Tumor Cell Proliferation"],"prefix":"10.3390","volume":"15","author":[{"given":"Xilei","family":"Guo","sequence":"first","affiliation":[{"name":"Key Laboratory of Natural Medicines of the Changbai Mountain, Ministry of Education, College of Pharmacy, Yanbian University, Yanji 133002, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiayu","family":"Gu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Natural Medicines of the Changbai Mountain, Ministry of Education, College of Pharmacy, Yanbian University, Yanji 133002, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiaxin","family":"Chu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Natural Medicines of the Changbai Mountain, Ministry of Education, College of Pharmacy, Yanbian University, Yanji 133002, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuanqi","family":"Duan","sequence":"additional","affiliation":[{"name":"Key Laboratory of Natural Medicines of the Changbai Mountain, Ministry of Education, College of Pharmacy, Yanbian University, Yanji 133002, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Debin","family":"Wang","sequence":"additional","affiliation":[{"name":"Yanbian Special Industry Development Center, Yanji 133002, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-5462-2750","authenticated-orcid":false,"given":"Hao","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, Jilin Medical University, Jilin 132013, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei","family":"Zhou","sequence":"additional","affiliation":[{"name":"Key Laboratory of Natural Medicines of the Changbai Mountain, Ministry of Education, College of Pharmacy, Yanbian University, Yanji 133002, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1721-7321","authenticated-orcid":false,"given":"Zhengyu","family":"Hu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Natural Medicines of the Changbai Mountain, Ministry of Education, College of Pharmacy, Yanbian University, Yanji 133002, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,9,28]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"3108","DOI":"10.1016\/j.ijbiomac.2022.10.085","article-title":"Recent advances in the bioactive polysaccharides and other key components from Phellinus spp. and their pharmacological effects: A review","volume":"222","author":"Zhang","year":"2022","journal-title":"Int. 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