{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T09:54:49Z","timestamp":1790848489727,"version":"4.1.0"},"reference-count":37,"publisher":"MDPI AG","issue":"19","license":[{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100007086","name":"Selcuk University Scientific Research and Project Coordination","doi-asserted-by":"publisher","award":["25401047"],"award-info":[{"award-number":["25401047"]}],"id":[{"id":"10.13039\/501100007086","id-type":"DOI","asserted-by":"publisher"}]},{"award":["25401047"],"award-info":[{"award-number":["25401047"]}],"id":[{"id":"https:\/\/ror.org\/045hgzm75","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Foods"],"abstract":"<jats:p>Refrigeration and freezing are commonly used preservation methods in the control of foodborne pathogens. However, the transcriptional responses of Salmonella Infantis to low-temperature conditions remain incompletely understood. In this study, the effect of low-temperature stress on the expression of pESI-related repA, ipf, and K88-like genes, as well as cspA, involved in cold shock response, and ompF, associated with environmental stress adaptation, was investigated in three pESI-positive, antibiotic-susceptible and three pESI-positive multi-antibiotic-resistant (MDR) S. Infantis isolates. Isolates were exposed to 4 \u00b0C and \u221220 \u00b0C conditions for 24 h, while isolates incubated at 37 \u00b0C were considered as the control group. Relative expression levels of genes were determined using the SYBR Green-based RT-qPCR method. Mean transcriptional patterns differed between the two selected isolate groups, most prominently at 4 \u00b0C. Relative to their respective 37 \u00b0C controls, all five target genes showed decreased mean expression in susceptible isolates but increased mean expression in MDR isolates at 4 \u00b0C. At \u221220 \u00b0C, group-mean expression was increased across all five targets in the MDR group, whereas the susceptible group showed decreased mean expression of the pESI-associated targets repA, ipfA, and klf, but modest increases in ompF and cspA. A significant temperature-by-phenotype interaction was detected for ipfA (p = 0.029). Between-group comparisons identified significant differences for the K88-like fimbrial locus (klf) and ompF at 4 \u00b0C. However, larger isolate groups and functional validation studies are needed to elucidate the biological significance of the identified expression changes.<\/jats:p>","DOI":"10.3390\/foods15193512","type":"journal-article","created":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T08:19:08Z","timestamp":1790842748000},"page":"3512","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Low-Temperature Transcriptional Responses in Selected pESI-Positive Salmonella Infantis Isolates with Contrasting Antimicrobial Resistance Phenotypes: An Exploratory Study"],"prefix":"10.3390","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8899-4537","authenticated-orcid":false,"given":"Arife Ezgi","family":"Telli","sequence":"first","affiliation":[{"name":"Department of Food Hygiene and Technology, Faculty of Veterinary Medicine, Selcuk University, Konya 42130, T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7549-8323","authenticated-orcid":false,"given":"Yusuf","family":"Bi\u00e7er","sequence":"additional","affiliation":[{"name":"Department of Food Hygiene and Technology, Faculty of Veterinary Medicine, Selcuk University, Konya 42130, T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4796-5961","authenticated-orcid":false,"given":"Gamze","family":"Turkal","sequence":"additional","affiliation":[{"name":"Department of Food Hygiene and Technology, Faculty of Veterinary Medicine, Selcuk University, Konya 42130, T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5181-7577","authenticated-orcid":false,"given":"Ahmet G\u00f6khan","family":"Co\u015fkun","sequence":"additional","affiliation":[{"name":"Food and Feed Branch Directorate, Bal\u0131kesir Provincial Directorate of Agriculture and Forestry, Bal\u0131kesir 10020, T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"writing-original-draft","vocabulary":"credit"},{"role":"formal-analysis","vocabulary":"credit"},{"role":"validation","vocabulary":"credit"},{"role":"software","vocabulary":"credit"}]},{"given":"Nihat","family":"Telli","sequence":"additional","affiliation":[{"name":"Department of Food Processing, Vocational School of Technical Sciences, Konya Technical University, Konya 42130, T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,10,1]]},"reference":[{"key":"ref_1","first-page":"73","article-title":"Salmonella Infantis","volume":"94","year":"2023","journal-title":"J. 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