{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T18:08:52Z","timestamp":1790791732521,"version":"4.1.0"},"reference-count":78,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2020,4,4]],"date-time":"2020-04-04T00:00:00Z","timestamp":1585958400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"SusMAPWaste, SMIS104323, Contract No. 89\/09.09.2016, from the Operational Program Competitiveness 2014-2020, project co financed from the European Regional Development Fund.","award":["89\/09.09.2016"],"award-info":[{"award-number":["89\/09.09.2016"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Foods"],"abstract":"<jats:p>Background: Momordica charantia Linn. (Cucurbitaceae), the wild variety of bitter melon and Morinda lucida Benth (Rubiaceae) were commonly used as a popular folk medicine in Benin. This research focused to measure the antioxidant and enzyme inhibitory effects of M. charantia and M. lucida leaves and their antidiabetic activity. Methods: Antioxidant activities were evaluated by micro-dilution technique using DPPH free radical scavenging activity and \u03b2-carotene-linoleate bleaching assay. The \u03b1-amylase inhibition assay was carried out utilizing the 3,5-dinitrosalicylic acid procedure, while \u03b2-glucosidase inhibition assay was demonstrated using as substrate p-nitrophenyl-\u03b2-D-glucopyranoside (PNPG). HPLC-DAD analysis was realized using a high-performance liquid chromatography systems with diode-array detector, L-3000. Results: Chlorogenic acid, epicatechin, daidzein, rutin, naringin, quercetin, naringenin and genistein were identified as polyphenol compounds in the both plants extract. Dichloromethane and ethyl acetate extracts showed a good \u03b1-amylase inhibitory activity (56.46 \u00b1 1.96% and 58.76 \u00b1 2.74% respectively). M. lucida methanolic extract has shown IC50 of 0.51 \u00b1 0.01 mg\/mL, which is the lowest for DPPH scavenging activity. M. lucida dichloromethane extract showed the highest inhibitory capacity of \u03b2-glucosidase activity (82.11. \u00b1 2.15%). Conclusion: These results justify some traditional medicinal uses of both plants. The purified fractions could be used in future formulations, possibly incorporated in functional foods to combat certain diseases.<\/jats:p>","DOI":"10.3390\/foods9040434","type":"journal-article","created":{"date-parts":[[2020,4,7]],"date-time":"2020-04-07T03:58:39Z","timestamp":1586231919000},"page":"434","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":71,"title":["Exploring Antioxidant and Enzymes (A-Amylase and B-Glucosidase) Inhibitory Activity of Morinda lucida and Momordica charantia Leaves from Benin"],"prefix":"10.3390","volume":"9","author":[{"given":"Michaelle","family":"Chokki","sequence":"first","affiliation":[{"name":"Laboratoire de Microbiologie et de Technologie Alimentaire, FAST, Universit\u00e9 d\u2019Abomey-Calavi, ISBA-Champ de foire, Cotonou 01BP: 526, Benin"},{"name":"Laboratoire de Biochimie et immunologie Appliqu\u00e9es (LABIA), Ecole Doctorale Sciences et Technologies, Universit\u00e9 Joseph KI-ZERBO, 03 BP 7021 Ouagadougou 03, Burkina-Faso"},{"name":"Department of Chemistry, Physics and Environment, \u201cDunarea de Jos\u201d University of Galati, 47 Domneasca Street, 800008 Galati, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2158-3877","authenticated-orcid":false,"given":"Mihaela","family":"Cud\u0103lbeanu","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Physics and Environment, \u201cDunarea de Jos\u201d University of Galati, 47 Domneasca Street, 800008 Galati, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3109-8509","authenticated-orcid":false,"given":"Cheikna","family":"Zongo","sequence":"additional","affiliation":[{"name":"Laboratoire de Biochimie et immunologie Appliqu\u00e9es (LABIA), Ecole Doctorale Sciences et Technologies, Universit\u00e9 Joseph KI-ZERBO, 03 BP 7021 Ouagadougou 03, Burkina-Faso"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Durand","family":"Dah-Nouvlessounon","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Physics and Environment, \u201cDunarea de Jos\u201d University of Galati, 47 Domneasca Street, 800008 Galati, Romania"},{"name":"Laboratory of Biology and Molecular Typing in Microbiology, Department of Biochemistry and Cell Biology, University of Abomey-Calavi, Faculty of Sciences and Techniques, Cotonou 05BP1604, Benin"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ioana Otilia","family":"Ghinea","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Physics and Environment, \u201cDunarea de Jos\u201d University of Galati, 47 Domneasca Street, 800008 Galati, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1745-5370","authenticated-orcid":false,"given":"Bianca","family":"Furdui","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Physics and Environment, \u201cDunarea de Jos\u201d University of Galati, 47 Domneasca Street, 800008 Galati, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7008-069X","authenticated-orcid":false,"given":"Robert","family":"Raclea","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Faculty of Natural Sciences, Imperial College London, London SW7 2AZ, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9090-290X","authenticated-orcid":false,"given":"Aly","family":"Savadogo","sequence":"additional","affiliation":[{"name":"Laboratoire de Biochimie et immunologie Appliqu\u00e9es (LABIA), Ecole Doctorale Sciences et Technologies, Universit\u00e9 Joseph KI-ZERBO, 03 BP 7021 Ouagadougou 03, Burkina-Faso"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8160-5954","authenticated-orcid":false,"given":"Lamine","family":"Baba-Moussa","sequence":"additional","affiliation":[{"name":"Laboratory of Biology and Molecular Typing in Microbiology, Department of Biochemistry and Cell Biology, University of Abomey-Calavi, Faculty of Sciences and Techniques, Cotonou 05BP1604, Benin"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5067-0323","authenticated-orcid":false,"given":"Sorin Marius","family":"Avamescu","sequence":"additional","affiliation":[{"name":"Department of Organic Chemistry, Biochemistry and Catalysis, Faculty of Chemistry, University of Bucharest, 90-92 Soseaua Panduri, Bucharest, Romania"},{"name":"University of Agronomic Science and Veterinary Medicine, 59 Marasti Blvd, 011464 Bucharest, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2126-0931","authenticated-orcid":false,"given":"Rodica Mihaela","family":"Dinica","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Physics and Environment, \u201cDunarea de Jos\u201d University of Galati, 47 Domneasca Street, 800008 Galati, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Farid","family":"Baba-Moussa","sequence":"additional","affiliation":[{"name":"Laboratoire de Microbiologie et de Technologie Alimentaire, FAST, Universit\u00e9 d\u2019Abomey-Calavi, ISBA-Champ de foire, Cotonou 01BP: 526, Benin"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2020,4,4]]},"reference":[{"key":"ref_1","first-page":"9452","article-title":"\u00c9tude ethnobotanique des plantes m\u00e9dicinales commercialis\u00e9es dans les march\u00e9s de la ville de Douala, 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