{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,28]],"date-time":"2026-01-28T04:36:19Z","timestamp":1769574979414,"version":"3.49.0"},"reference-count":59,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2020,12,3]],"date-time":"2020-12-03T00:00:00Z","timestamp":1606953600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Foods"],"abstract":"<jats:p>Highly branched neo-fructans (agavins) are natural prebiotics found in Agave plants, with a large capacity to mitigate the development of obesity and metabolic syndrome. Here, we investigated the impact of agavins intake on gut microbiota modulation and their metabolites as well as their effect on metabolic endotoxemia and low-grade inflammation in mice fed high-fat diet. Mice were fed with a standard diet (ST) and high-fat diet (HF) alone or plus an agavins supplement (HF+A) for ten weeks. Gut microbiota composition, fecal metabolite profiles, lipopolysaccharides (LPS), pro-inflammatory cytokines, and systemic effects were analyzed. Agavins intake induced substantial changes in gut microbiota composition, enriching Bacteroides, Parabacteroides, Prevotella, Allobaculum, and Akkermansia genus (LDA &gt; 3.0). l-leucine, l-valine, uracil, thymine, and some fatty acids were identified as possible biomarkers for this prebiotic supplement. As novel findings, agavins supplementation significantly decreased LPS and pro-inflammatory (IL-1\u03b1, IL-1\u03b2, and TNF-\u03b1; p &lt; 0.05) cytokines levels in portal vein. In addition, lipid droplets content in the liver and adipocytes size also decreased with agavins consumption. In conclusion, agavins supplementation mitigate metabolic endotoxemia and low-grade inflammation in association with gut microbiota regulation and their metabolic products, thus inducing beneficial responses on metabolic disorders in high-fat diet-fed mice.<\/jats:p>","DOI":"10.3390\/foods9121792","type":"journal-article","created":{"date-parts":[[2020,12,3]],"date-time":"2020-12-03T20:09:40Z","timestamp":1607026180000},"page":"1792","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":29,"title":["Highly Branched Neo-Fructans (Agavins) Attenuate Metabolic Endotoxemia and Low-Grade Inflammation in Association with Gut Microbiota Modulation on High-Fat Diet-Fed Mice"],"prefix":"10.3390","volume":"9","author":[{"given":"Alicia","family":"Huazano-Garc\u00eda","sequence":"first","affiliation":[{"name":"Departamento de Biotecnolog\u00eda y Bioqu\u00edmica, Centro de Investigaci\u00f3n y de Estudios Avanzados del IPN, Unidad Irapuato, Guanajuato 36821, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mar\u00eda Blanca","family":"Silva-Adame","sequence":"additional","affiliation":[{"name":"Departamento de Biotecnolog\u00eda y Bioqu\u00edmica, Centro de Investigaci\u00f3n y de Estudios Avanzados del IPN, Unidad Irapuato, Guanajuato 36821, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3037-1457","authenticated-orcid":false,"given":"Juan","family":"V\u00e1zquez-Mart\u00ednez","sequence":"additional","affiliation":[{"name":"Departamento de Biotecnolog\u00eda y Bioqu\u00edmica, Centro de Investigaci\u00f3n y de Estudios Avanzados del IPN, Unidad Irapuato, Guanajuato 36821, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2745-6783","authenticated-orcid":false,"given":"Argel","family":"Gastelum-Arellanez","sequence":"additional","affiliation":[{"name":"C\u00e1tedra CONACYT, Centro de Innovaci\u00f3n Aplicada en Tecnolog\u00edas Competitivas A.C. (CIATEC AC), Le\u00f3n 37545, Guanajuato, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6179-3233","authenticated-orcid":false,"given":"Lino","family":"S\u00e1nchez-Segura","sequence":"additional","affiliation":[{"name":"Departamento de Ingenier\u00eda Gen\u00e9tica, Centro de Investigaci\u00f3n y de Estudios Avanzados del IPN, Unidad Irapuato, Guanajuato 36821, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0660-8119","authenticated-orcid":false,"given":"Mercedes G.","family":"L\u00f3pez","sequence":"additional","affiliation":[{"name":"Departamento de Biotecnolog\u00eda y Bioqu\u00edmica, Centro de Investigaci\u00f3n y de Estudios Avanzados del IPN, Unidad Irapuato, Guanajuato 36821, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2020,12,3]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"673","DOI":"10.1007\/s40273-014-0243-x","article-title":"The epidemiology of obesity: A big picture","volume":"33","author":"Hruby","year":"2015","journal-title":"Pharmacoeconomics"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"860","DOI":"10.1038\/nature05485","article-title":"Inflammation and metabolic disorders","volume":"444","author":"Hotamisligil","year":"2006","journal-title":"Nature"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"415","DOI":"10.1146\/annurev-immunol-031210-101322","article-title":"Inflammatory mechanisms in obesity","volume":"29","author":"Gregor","year":"2011","journal-title":"Annu. 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