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Gelation of chitosan with DEs was significantly slower than with GA, allowing formation of cryogels with larger pores and higher permeability, more suitable for flow-through applications and cell culturing. Increased hydration of the cross-links with increased DE chain length weakened intermolecular hydrogen bonding in chitosan and improved cryogel elasticity. At high cross-linking ratios (DE:chitosan 1:4), the toughness and compressive strength of the cryogels decreased in the order EGDE &gt; BDDE &gt; PEGDE. By varying the DE chain length and concentration, permeable chitosan cryogels with elasticity moduli from 10.4 \u00b1 0.8 to 41 \u00b1 3 kPa, toughness from 2.68 \u00b1 0.5 to 8.3 \u00b1 0.1 kJ\/m3, and compressive strength at 75% strain from 11 \u00b1 2 to 33 \u00b1 4 kPa were fabricated. Susceptibility of cryogels to enzymatic hydrolysis was identified as the parameter most sensitive to cross-linking conditions. Weight loss of cryogels increased with increased DE chain length, and degradation rate of PEGDE-cross-linked chitosan decreased 612-fold, when the cross-linker concentration increased 20-fold.<\/jats:p>","DOI":"10.3390\/gels10070483","type":"journal-article","created":{"date-parts":[[2024,7,22]],"date-time":"2024-07-22T17:36:04Z","timestamp":1721669764000},"page":"483","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":24,"title":["Tuning Mechanical Properties, Swelling, and Enzymatic Degradation of Chitosan Cryogels Using Diglycidyl Ethers of Glycols with Different Chain Length as Cross-Linkers"],"prefix":"10.3390","volume":"10","author":[{"given":"Yuliya","family":"Privar","sequence":"first","affiliation":[{"name":"Institute of Chemistry Far Eastern Branch of the Russian Academy of Sciences, 159, Prosp. 100-Letiya Vladivostoka, 690022 Vladivostok, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna","family":"Skatova","sequence":"additional","affiliation":[{"name":"Institute of Chemistry Far Eastern Branch of the Russian Academy of Sciences, 159, Prosp. 100-Letiya Vladivostoka, 690022 Vladivostok, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mariya","family":"Maiorova","sequence":"additional","affiliation":[{"name":"A.V. Zhirmunsky National Scientific Center of Marine Biology, Far Eastern Branch of Russian Academy of Sciences, 17, Palchevskogo Street, 690041 Vladivostok, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5306-2542","authenticated-orcid":false,"given":"Alexey","family":"Golikov","sequence":"additional","affiliation":[{"name":"Institute of Chemistry Far Eastern Branch of the Russian Academy of Sciences, 159, Prosp. 100-Letiya Vladivostoka, 690022 Vladivostok, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0257-748X","authenticated-orcid":false,"given":"Andrey","family":"Boroda","sequence":"additional","affiliation":[{"name":"A.V. 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Molecules, 25.","DOI":"10.3390\/molecules25143156"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"121408","DOI":"10.1016\/j.carbpol.2023.121408","article-title":"Recent Advances in Biopolymer-Based Hydrogels and Their Potential Biomedical Applications","volume":"323","author":"Patel","year":"2024","journal-title":"Carbohydr. Polym."},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Fan, P., Zeng, Y., Zaldivar-Silva, D., Ag\u00fcero, L., and Wang, S. (2023). Chitosan-Based Hemostatic Hydrogels: The Concept, Mechanism, Application, and Prospects. Molecules, 28.","DOI":"10.3390\/molecules28031473"},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Lv, S., Zhang, S., Zuo, J., Liang, S., Yang, J., Wang, J., and Wei, D. (2023). Progress in Preparation and Properties of Chitosan-Based Hydrogels. Int. J. Biol. Macromol., 242.","DOI":"10.1016\/j.ijbiomac.2023.124915"},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Xu, X., He, C., Luo, F., Wang, H., and Peng, Z. (2021). Transparent, Conductive Hydrogels with High Mechanical Strength and Toughness. Polymers, 13.","DOI":"10.3390\/polym13122004"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1016\/j.jare.2022.04.005","article-title":"Polysaccharide-Based Biopolymer Hydrogels for Heavy Metal Detection and Adsorption","volume":"44","author":"Zhao","year":"2023","journal-title":"J. Adv. Res."},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Dinu, I.A., Ghimici, L., and Raschip, I.E. (2022). Macroporous 3D Chitosan Cryogels for Fastac 10EC Pesticide Adsorption and Antibacterial Applications. Polymers, 14.","DOI":"10.3390\/polym14153145"},{"key":"ref_8","first-page":"29","article-title":"Modification of chitosan cryogels by pyridoxal phosphate to improve sorption capacity","volume":"43","author":"Veleshko","year":"2012","journal-title":"Fibre Chem."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"52","DOI":"10.1080\/01614940.2021.1904543","article-title":"Copper Species Supported in Polysaccharide-Based Materials: From Preparation to Application in Catalysis","volume":"65","author":"Gularte","year":"2023","journal-title":"Catal. Rev."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"1800114","DOI":"10.1002\/adtp.201800114","article-title":"Latest Advances in Cryogel Technology for Biomedical Applications","volume":"2","author":"Memic","year":"2019","journal-title":"Adv. Ther."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"910","DOI":"10.55730\/1300-0527.3586","article-title":"Cryogelation Reactions and Cryogels: Principles and Challenges","volume":"47","author":"Okay","year":"2023","journal-title":"Turk. J. Chem."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"88","DOI":"10.1016\/S1369-7021(11)70058-X","article-title":"Biomaterials & Scaffolds for Tissue Engineering","volume":"14","year":"2011","journal-title":"Mater. Today"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"45751","DOI":"10.1038\/srep45751","article-title":"Chitosan Promotes Cancer Progression and Stem Cell Properties in Association with Wnt Signaling in Colon and Hepatocellular Carcinoma Cells","volume":"8","author":"Chang","year":"2017","journal-title":"Sci. Rep."},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Savina, I.N., Zoughaib, M., and Yergeshov, A.A. (2021). Design and Assessment of Biodegradable Macroporous Cryogels as Advanced Tissue Engineering and Drug Carrying Materials. Gels, 7.","DOI":"10.3390\/gels7030079"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"558","DOI":"10.1016\/j.ijbiomac.2022.01.029","article-title":"Crosslinkers for Polysaccharides and Proteins: Synthesis Conditions, Mechanisms, and Crosslinking Efficiency, a Review","volume":"202","author":"Alavarse","year":"2022","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Jayachandran, B., Parvin, T.N., Alam, M.M., Chanda, K., and Mm, B. (2022). Insights on Chemical Crosslinking Strategies for Proteins. 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C"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"540","DOI":"10.1098\/rsif.2010.0455","article-title":"Supermacroprous Chitosan\u2013Agarose\u2013Gelatin Cryogels: In Vitro Characterization and in Vivo Assessment for Cartilage Tissue Engineering","volume":"8","author":"Bhat","year":"2011","journal-title":"J. R. Soc. Interface"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"663","DOI":"10.1016\/j.jbiosc.2012.07.005","article-title":"Cell Proliferation on Three-Dimensional Chitosan\u2013Agarose\u2013Gelatin Cryogel Scaffolds for Tissue Engineering Applications","volume":"114","author":"Bhat","year":"2012","journal-title":"J. Biosci. Bioeng."},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Carmona, P., Tasici, A.M., Sande, S.A., Knudsen, K.D., and Nystr\u00f6m, B. (2021). Glyceraldehyde as an Efficient Chemical Crosslinker Agent for the Formation of Chitosan Hydrogels. Gels, 7.","DOI":"10.3390\/gels7040186"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"951","DOI":"10.1002\/macp.1989.021900504","article-title":"Chitosan Gels, 3. The Formation of Gels by Reaction of Chitosan with Glutaraldehyde","volume":"190","author":"Roberts","year":"1989","journal-title":"Macromol. Chem."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"2682","DOI":"10.1039\/c3tb20280a","article-title":"Cryogels for Biomedical Applications","volume":"1","author":"Henderson","year":"2013","journal-title":"J. Mater. Chem. B"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"406","DOI":"10.1016\/j.actbio.2008.07.009","article-title":"Synthesis and Characterization of Elastic and Macroporous Chitosan-Gelatin Cryogels for Tissue Engineering","volume":"5","author":"Kathuria","year":"2009","journal-title":"Acta Biomater."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"161","DOI":"10.1016\/j.jmbbm.2014.10.010","article-title":"Improving Hydrogels\u2019 Toughness by Increasing the Dissipative Properties of Their Network","volume":"41","author":"Moghadam","year":"2015","journal-title":"J. Mech. Behav. Biomed. Mater."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"131","DOI":"10.1016\/j.ejps.2016.06.010","article-title":"Study of the Effect of Mixing Approach on Cross-Linking Efficiency of Hyaluronic Acid-Based Hydrogel Cross-Linked with 1,4-Butanediol Diglycidyl Ether","volume":"91","author":"Neubert","year":"2016","journal-title":"Eur. J. Pharm. Sci."},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Zerbinati, N., Sommatis, S., Maccario, C., Capillo, M.C., Grimaldi, G., Alonci, G., Protasoni, M., Rauso, R., and Mocchi, R. (2021). Toward Physicochemical and Rheological Characterization of Different Injectable Hyaluronic Acid Dermal Fillers Cross-Linked with Polyethylene Glycol Diglycidyl Ether. Polymers, 13.","DOI":"10.3390\/polym13060948"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"137","DOI":"10.1615\/JLongTermEffMedImplants.v27.i2-4.40","article-title":"Bioprostheses and Its Alternative Fixation","volume":"27","author":"Hendriks","year":"2017","journal-title":"J. Long. Term. Eff. Med. Implants"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"124","DOI":"10.1016\/j.carbpol.2018.06.061","article-title":"Hydrogels for Biomedical Applications from Glycol Chitosan and PEG Diglycidyl Ether Exhibit Pro-Angiogenic and Antibacterial Activity","volume":"198","author":"Tripodo","year":"2018","journal-title":"Carbohydr. Polym."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"1624","DOI":"10.1002\/pi.5424","article-title":"Synthesis of Highly Swellable Hydrogels of Water-Soluble Carboxymethyl Chitosan and Poly(Ethylene Glycol)","volume":"66","author":"Edlund","year":"2017","journal-title":"Polym. Int."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"742","DOI":"10.1002\/jbm.a.30489","article-title":"Crosslinked Chitosan: Its Physical Properties and the Effects of Matrix Stiffness on Chondrocyte Cell Morphology and Proliferation","volume":"75","author":"Subramanian","year":"2005","journal-title":"J. Biomed. Mater. Res.-Part A"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"107","DOI":"10.1002\/mabi.200400140","article-title":"PEG-Grafted Chitosan as an Injectable Thermoreversible Hydrogel","volume":"5","author":"Bhattarai","year":"2005","journal-title":"Macromol. Biosci."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"2823","DOI":"10.1002\/app.27546","article-title":"Preparation and Characterization of Poly(Ethylene Glycol) Crosslinked Chitosan Films","volume":"107","author":"Kiuchi","year":"2008","journal-title":"J. Appl. Polym. Sci."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"1635","DOI":"10.1021\/acs.biomac.8b01817","article-title":"Chitosan Gels and Cryogels Cross-Linked with Diglycidyl Ethers of Ethylene Glycol and Polyethylene Glycol in Acidic Media","volume":"20","author":"Privar","year":"2019","journal-title":"Biomacromolecules"},{"key":"ref_35","doi-asserted-by":"crossref","unstructured":"Privar, Y., Boroda, A., Pestov, A., Kazantsev, D., Malyshev, D., Skatova, A., and Bratskaya, S. (2023). Chitosan Cryogels Cross-Linked with 1,1,3-Triglycidyloxypropane: Mechanical Properties and Cytotoxicity for Cancer Cell 3D Cultures. Biomimetics, 8.","DOI":"10.3390\/biomimetics8020228"},{"key":"ref_36","doi-asserted-by":"crossref","unstructured":"Boroda, A., Privar, Y., Maiorova, M., Beleneva, I., Eliseikina, M., Skatova, A., Marinin, D., and Bratskaya, S. (2022). Chitosan versus Carboxymethyl Chitosan Cryogels: Bacterial Colonization, Human Embryonic Kidney 293T Cell Culturing and Co-Culturing. Int. J. Mol. Sci., 23.","DOI":"10.3390\/ijms232012276"},{"key":"ref_37","doi-asserted-by":"crossref","unstructured":"Boroda, A., Privar, Y., Maiorova, M., Skatova, A., and Bratskaya, S. (2022). Sponge-like Scaffolds for Colorectal Cancer 3D Models: Substrate-Driven Difference in Micro-Tumors Morphology. Biomimetics, 7.","DOI":"10.3390\/biomimetics7020056"},{"key":"ref_38","first-page":"164","article-title":"Removal of alizarin red by supermacroporous cross-linked chitosan monolith sorbents","volume":"24","author":"Privar","year":"2019","journal-title":"Prog. Chem. Appl. Chitin Its Deriv."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"269","DOI":"10.1016\/j.polymdegradstab.2018.09.005","article-title":"Accessibility of Chitin and Chitosan in Enzymatic Hydrolysis: A Review","volume":"156","author":"Poshina","year":"2018","journal-title":"Polym. Degrad. Stab."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"131","DOI":"10.1007\/PL00000584","article-title":"Human Enzymatic Activities Related to the Therapeutic Administration of Chitin Derivatives","volume":"53","author":"Muzzarelli","year":"1997","journal-title":"Cell. Mol. Life Sci."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"453","DOI":"10.1163\/156856208783719482","article-title":"In Vitro and in Vivo Degradation Behavior of Acetylated Chitosan Porous Beads","volume":"19","author":"Lim","year":"2008","journal-title":"J. Biomater. Sci."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"e01684","DOI":"10.1016\/j.heliyon.2019.e01684","article-title":"Degradability of Chitosan Micro\/Nanoparticles for Pulmonary Drug Delivery","volume":"5","author":"Islam","year":"2019","journal-title":"Heliyon"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"260","DOI":"10.1016\/j.carbpol.2009.11.022","article-title":"Preparation and Characterization of PEG-Cross-Linked Chitosan Hydrogel Films with Controllable Swelling and Enzymatic Degradation Behavior","volume":"80","author":"Tanuma","year":"2010","journal-title":"Carbohydr. Polym."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"155","DOI":"10.1016\/j.actbio.2004.12.001","article-title":"Crosslinked \u03b1-Elastin Biomaterials: Towards a Processable Elastin Mimetic Scaffold","volume":"1","author":"Leach","year":"2005","journal-title":"Acta Biomater."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"84","DOI":"10.1016\/j.carbpol.2014.02.020","article-title":"Characterization and Properties of Carboxymethyl Cellulose Hydrogels Crosslinked by Polyethylene Glycol","volume":"106","author":"Kono","year":"2014","journal-title":"Carbohydr. Polym."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"1150","DOI":"10.1134\/S0965545X1112011X","article-title":"Effect of Polymer-Precursor Molecular Mass on the Formation and Properties of Covalently Crosslinked Chitosan Cryogels","volume":"53","author":"Nikonorov","year":"2011","journal-title":"Polym. Sci.-Ser. A"},{"key":"ref_47","unstructured":"(2024, February 05). Chemical Properties of Oxirane, 2,2\u2032-[1,4-Butanediylbis(oxymethylene)]bis. Available online: https:\/\/www.chemeo.com\/cid\/12-593-0\/Oxirane-2-2-1-4-butanediylbis-oxymethylene-bis."},{"key":"ref_48","unstructured":"(2024, February 05). Chemical Properties of Ethylene Glycol Diglycidyl Ether. Available online: https:\/\/www.chemeo.com\/cid\/22-158-2\/Ethylene-glycol-diglycidyl-ether."},{"key":"ref_49","doi-asserted-by":"crossref","unstructured":"Tomaz, A.F., de Carvalho, S.M.S., Barbosa, R.C., Silva, S.M.L., Gutierrez, M.A.S., de Lima, A.G.B., and Fook, M.V.L. (2018). Ionically Crosslinked Chitosan Membranes Used as Drug Carriers for Cancer Therapy Application. Materials, 11.","DOI":"10.3390\/ma11102051"},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"19165","DOI":"10.1038\/s41598-019-55214-7","article-title":"PEG Grafted Chitosan Scaffold for Dual Growth Factor Delivery for Enhanced Wound Healing","volume":"9","author":"Vijayan","year":"2019","journal-title":"Sci. Rep."},{"key":"ref_51","doi-asserted-by":"crossref","unstructured":"Pretsch, E., B\u00fchlmann, P., and Affolter, C. (2000). Structure Determination of Organic Compounds: Tables of Spectral Data, Springer.","DOI":"10.1007\/978-3-662-04201-4"},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"331","DOI":"10.1016\/j.watres.2014.09.043","article-title":"Bisphenol Diglycidyl Ethers and Bisphenol A and Their Hydrolysis in Drinking Water","volume":"72","author":"Lane","year":"2015","journal-title":"Water Res."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"3477","DOI":"10.1002\/jbm.a.35015","article-title":"A Factorial Analysis of the Combined Effects of Hydrogel Fabrication Parameters on the in Vitro Swelling and Degradation of Oligo(Poly(Ethylene Glycol) Fumarate) Hydrogels","volume":"102","author":"Lam","year":"2014","journal-title":"J. Biomed. Mater. Res.-Part A"},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"94","DOI":"10.1021\/ie50553a029","article-title":"Glycidyl Ether Reactions with Amines","volume":"48","author":"Shechter","year":"1956","journal-title":"Ind. Eng. Chem."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"489","DOI":"10.1070\/RC2002v071n06ABEH000720","article-title":"Cryogels on the Basis of Natural and Synthetic Polymers: Preparation, Properties and Application","volume":"71","author":"Lozinsky","year":"2002","journal-title":"Russ. Chem. Rev."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"5937","DOI":"10.1021\/acsomega.1c06178","article-title":"Superamphiphilic Chitosan Cryogels for Continuous Flow Separation of Oil-In-Water Emulsions","volume":"7","author":"Gao","year":"2022","journal-title":"ACS Omega"},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"50","DOI":"10.1016\/j.ces.2019.02.026","article-title":"Control of Ice Crystal Growth and Its Effect on Porous Structure of Chitosan Cryogels","volume":"201","author":"Zhang","year":"2019","journal-title":"Chem. Eng. Sci."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"907","DOI":"10.1007\/s00216-007-1745-6","article-title":"Monolithic Macroporous Albumin\/Chitosan Cryogel Structure: A New Matrix for Enzyme Immobilization","volume":"390","author":"Plieva","year":"2008","journal-title":"Anal. Bioanal. Chem."},{"key":"ref_59","doi-asserted-by":"crossref","unstructured":"Berillo, D., and Arysbek, A. (2023). Chitosan Glutaraldegyde Cryogels for Wastewater Treatment and Extraction of Silver Nanoparticles. Processes, 11.","DOI":"10.3390\/pr11061661"},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"828","DOI":"10.1134\/S0965545X10080092","article-title":"Synthesis and Characteristics of Cryogels of Chitosan Crosslinked by Glutaric Aldehyde","volume":"52","author":"Nikonorov","year":"2010","journal-title":"Polym. Sci.-Ser. A"},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"122172","DOI":"10.1016\/j.molliq.2023.122172","article-title":"Hydration Sphere Structure of Architectural Molecules: Polyethylene Glycol and Polyoxymethylene Oligomers","volume":"384","author":"Rozza","year":"2023","journal-title":"J. Mol. Liq."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"411","DOI":"10.1080\/00914037.2018.1452225","article-title":"Tough and Hierarchical Porous Cryogel Scaffolds Preparation Using N-Butanol as a Non-Solvent","volume":"68","author":"Sen","year":"2019","journal-title":"Int. J. Polym. Mater. Polym. Biomater."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"104372","DOI":"10.1016\/j.reactfunctpolym.2019.104372","article-title":"Advances in Porous Chitosan-Based Composite Hydrogels: Synthesis and Applications","volume":"146","author":"Dragan","year":"2020","journal-title":"React. Funct. Polym."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"267","DOI":"10.1080\/10236669908009742","article-title":"Solubilization of Chitosan in Strong Acid Medium","volume":"5","author":"Rinaudo","year":"1999","journal-title":"Int. J. Polym. Anal. Charact."},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"149","DOI":"10.1016\/0141-8130(94)90042-6","article-title":"Viscosity and Flow Properties of Concentrated Solutions of Chitosan with Different Degrees of Deacetylation","volume":"16","author":"Wang","year":"1994","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"2437","DOI":"10.1016\/j.polymer.2011.03.053","article-title":"Increasing Viscosity in Entangled Polyelectrolyte Solutions by the Addition of Salt","volume":"52","author":"Wyatt","year":"2011","journal-title":"Polymer"},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"2302041","DOI":"10.1002\/admt.202302041","article-title":"DLP-Printable Porous Cryogels for 3D Soft Tactile Sensing","volume":"9","author":"Cafiso","year":"2024","journal-title":"Adv. Mater. Technol."},{"key":"ref_68","doi-asserted-by":"crossref","unstructured":"Rege, A. (2021). Constitutive Modeling of the Densification Behavior in Open-Porous Cellular Solids. Materials, 14.","DOI":"10.3390\/ma14112731"},{"key":"ref_69","doi-asserted-by":"crossref","unstructured":"Xu, K., Ganapathy, K., Andl, T., Wang, Z., Copland, J.A., Chakrabarti, R., and Florczyk, S.J. (2019). 3D Porous Chitosan-Alginate Scaffold Stiffness Promotes Differential Responses in Prostate Cancer Cell Lines. Biomaterials, 217.","DOI":"10.1016\/j.biomaterials.2019.119311"},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"1326","DOI":"10.1002\/pen.26619","article-title":"Comparative Study of the Effect of Cross-Linking Degree on Chitosan Hydrogels Synthesized with Low and Medium Molecular Weight Chitosan","volume":"64","author":"Ceylan","year":"2024","journal-title":"Polym. Eng. Sci."},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"413","DOI":"10.1016\/j.jmbbm.2014.09.014","article-title":"In Vitro Assessment of Three Dimensional Dense Chitosan-Based Structures to Be Used as Bioabsorbable Implants","volume":"40","author":"Sirgado","year":"2014","journal-title":"J. Mech. Behav. Biomed. Mater."},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"1902","DOI":"10.1002\/app.30277","article-title":"Characterization and Enzymatic Degradation of PEG-Cross-Linked Chitosan Hydrogel Films","volume":"114","author":"Tanuma","year":"2009","journal-title":"J. Appl. Polym. Sci."},{"key":"ref_73","doi-asserted-by":"crossref","first-page":"713","DOI":"10.1016\/0020-711X(88)90167-X","article-title":"Non-Identity of Human Plasma Lysozyme and 4-Methylumbelliferyl-Tetra-N-Acetyl-\u03b2-d-Chitotetraoside Hydrolase","volume":"20","author":"Inaba","year":"1988","journal-title":"Int. J. Biochem."},{"key":"ref_74","doi-asserted-by":"crossref","first-page":"113","DOI":"10.1016\/0020-711X(93)90497-3","article-title":"Evaluation on the Hydrolysis of Methylumbelliferyl-Tetra-N-Acetylchitotetraoside by Various Glucosidases. A Comparative Study","volume":"25","author":"Scharpe","year":"1993","journal-title":"Int. J. Biochem."},{"key":"ref_75","doi-asserted-by":"crossref","first-page":"381","DOI":"10.1134\/S0003683814040061","article-title":"Hydrolysis of Chitozan with an Enzyme Complex from Myceliophthora sp.","volume":"50","author":"Khasanova","year":"2014","journal-title":"Appl. Biochem. Microbiol."},{"key":"ref_76","doi-asserted-by":"crossref","first-page":"671","DOI":"10.1038\/nmeth.2089","article-title":"NIH Image to ImageJ: 25 Years of Image Analysis","volume":"9","author":"Schneider","year":"2012","journal-title":"Nat. Methods"},{"key":"ref_77","doi-asserted-by":"crossref","unstructured":"Zvukova, N.D., Klimova, T.P., Ivanov, R.V., Ryabev, A.N., Tsiskarashvili, A.V., and Lozinsky, V.I. (2019). Cryostructuring of Polymeric Systems. 52. Properties, Microstructure and an Example of a Potential Biomedical Use of the Wide-Pore Alginate Cryostructurates. Gels, 5.","DOI":"10.3390\/gels5020025"},{"key":"ref_78","doi-asserted-by":"crossref","unstructured":"Bratskaya, S., Skatova, A., Privar, Y., Boroda, A., Kantemirova, E., Maiorova, M., and Pestov, A. (2021). Stimuli-Responsive Dual Cross-Linked N-Carboxyethylchitosan Hydrogels with Tunable Dissolution Rate. 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