{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T13:01:01Z","timestamp":1792069261748,"version":"4.4.0"},"reference-count":65,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2024,10,22]],"date-time":"2024-10-22T00:00:00Z","timestamp":1729555200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Directorate of Research and Development, Univer-sitas Indonesia under HIBAH PUTI 2022","award":["NKB-1433\/UN2.RST\/HKP.05.00\/2022"],"award-info":[{"award-number":["NKB-1433\/UN2.RST\/HKP.05.00\/2022"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Gels"],"abstract":"<jats:p>Connective tissue grafts for gingival recession treatment present significant challenges as they require an additional surgical site, leading to increased morbidity, extended operative times, and a more painful postoperative recovery for patients. Gelatin contains the arginine\u2013glycine\u2013aspartic acid (RGD) sequence, which supports cell adhesion and interactions. The development of gelatin hydrogels holds significant promise due to their biocompatibility, ease of customization, and structural resemblance to the extracellular matrix, making them a potential candidate for gingival regeneration. This study aimed to assess the physical and biological properties of crosslinked gelatin hydrogels using EDC\/NHS with two crosslinker concentrations (GelCL12 and GelCL24) and compare these to non-crosslinked gelatin. Both groups underwent morphological, rheological, and chemical analysis. Biological assessments were conducted to evaluate human gingival fibroblast (HGF) proliferation, migration, and COL1 expression in response to the scaffolds. The crosslinked gelatin group exhibited greater interconnectivity and better physical characteristics without displaying cytotoxic effects on the cells. FTIR analysis revealed no significant chemical differences between the groups. Notably, the GelCL12 group significantly enhanced HGF migration and upregulated COL1 expression. Overall, GelCL12 met the required physical characteristics and biocompatibility, making it a promising scaffold for future gingival tissue regeneration applications.<\/jats:p>","DOI":"10.3390\/gels10110674","type":"journal-article","created":{"date-parts":[[2024,10,22]],"date-time":"2024-10-22T06:11:25Z","timestamp":1729577485000},"page":"674","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["The Effect of Carbodiimide Crosslinkers on Gelatin Hydrogel as a Potential Biomaterial for Gingival Tissue Regeneration"],"prefix":"10.3390","volume":"10","author":[{"given":"Dimas Ilham","family":"Hutomo","sequence":"first","affiliation":[{"name":"Doctoral Program, Faculty of Dentistry, Universitas Indonesia, Jakarta 10430, Indonesia"},{"name":"Department of Periodontology, Faculty of Dentistry, Universitas Indonesia, Jakarta 10430, Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fathia Agzarine","family":"Deandra","sequence":"additional","affiliation":[{"name":"Postgraduate Program in Periodontology, Department of Periodontology, Faculty of Dentistry, Universitas Indonesia, Jakarta 10430, Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ketherin","family":"Ketherin","sequence":"additional","affiliation":[{"name":"Postgraduate Program in Periodontology, Department of Periodontology, Faculty of Dentistry, Universitas Indonesia, Jakarta 10430, Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7062-9099","authenticated-orcid":false,"given":"Elena","family":"Garc\u00eda-Gareta","sequence":"additional","affiliation":[{"name":"Multiscale in Mechanical and Biological Engineering, Aragon Institute of Engineering Research (I3A), Aragon Institute of Health Research (IIS Aragon), University of Zaragoza, 50009 Zaragoza, Spain"},{"name":"Division of Biomaterials and Tissue Engineering, UCL Eastman Dental Institute, University College London, London WC1E 6DE, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2512-2579","authenticated-orcid":false,"given":"Endang Winiati","family":"Bachtiar","sequence":"additional","affiliation":[{"name":"Department of Oral Biology, Faculty of Dentistry, Universitas Indonesia, Jakarta 10430, Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6991-0497","authenticated-orcid":false,"given":"Lisa","family":"Amir","sequence":"additional","affiliation":[{"name":"Department of Oral Biology, Faculty of Dentistry, Universitas Indonesia, Jakarta 10430, Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8767-0941","authenticated-orcid":false,"given":"Fatimah Maria","family":"Tadjoedin","sequence":"additional","affiliation":[{"name":"Department of Periodontology, Faculty of Dentistry, Universitas Indonesia, Jakarta 10430, Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adityo","family":"Widaryono","sequence":"additional","affiliation":[{"name":"Department of Periodontology, Faculty of Dentistry, Universitas Indonesia, Jakarta 10430, Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Natalina","family":"Haerani","sequence":"additional","affiliation":[{"name":"Department of Periodontology, Faculty of Dentistry, Universitas Indonesia, Jakarta 10430, Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert","family":"Lessang","sequence":"additional","affiliation":[{"name":"Department of Periodontology, Faculty of Dentistry, Universitas Indonesia, Jakarta 10430, Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuniarti","family":"Soeroso","sequence":"additional","affiliation":[{"name":"Department of Periodontology, Faculty of Dentistry, Universitas Indonesia, Jakarta 10430, Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,10,22]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Hutomo, D.I., Amir, L., Suniarti, D.F., Bachtiar, E.W., and Soeroso, Y. 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