{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,4]],"date-time":"2026-09-04T05:05:25Z","timestamp":1788498325404,"version":"build-2803163510"},"reference-count":44,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2024,10,25]],"date-time":"2024-10-25T00:00:00Z","timestamp":1729814400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Naresuan University (NU)","award":["FRB650022\/0179"],"award-info":[{"award-number":["FRB650022\/0179"]}]},{"name":"Naresuan University (NU)","award":["R2565B019"],"award-info":[{"award-number":["R2565B019"]}]},{"name":"National Science, Research and Innovation Fund (NSRF)","award":["FRB650022\/0179"],"award-info":[{"award-number":["FRB650022\/0179"]}]},{"name":"National Science, Research and Innovation Fund (NSRF)","award":["R2565B019"],"award-info":[{"award-number":["R2565B019"]}]},{"name":"Center of Excellence for Innovation in Chemistry (PERCH-CIC)","award":["FRB650022\/0179"],"award-info":[{"award-number":["FRB650022\/0179"]}]},{"name":"Center of Excellence for Innovation in Chemistry (PERCH-CIC)","award":["R2565B019"],"award-info":[{"award-number":["R2565B019"]}]},{"name":"Office of the Ministry of Higher Education, Science, Research and Innovation","award":["FRB650022\/0179"],"award-info":[{"award-number":["FRB650022\/0179"]}]},{"name":"Office of the Ministry of Higher Education, Science, Research and Innovation","award":["R2565B019"],"award-info":[{"award-number":["R2565B019"]}]},{"name":"Faculty of Pharmaceutical Sciences, Naresuan University","award":["FRB650022\/0179"],"award-info":[{"award-number":["FRB650022\/0179"]}]},{"name":"Faculty of Pharmaceutical Sciences, Naresuan University","award":["R2565B019"],"award-info":[{"award-number":["R2565B019"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Gels"],"abstract":"<jats:p>This study explores the efficacy of a vancomycin-loaded silk fibroin\/calcium phosphate\/methylcellulose-based in situ thermosensitive hydrogel (VM-SF\/CaP\/MC) in promoting the osteogenic differentiation of preosteoblast cells. Three VM-SF\/CaP\/MC formulations with varying low (L) and high (H) concentrations of silk fibroin (SF) and calcium phosphate (CaP) were prepared: VM-HSF\/LCaP\/MC, VM-LSF\/HCaP\/MC, and VM-HSF\/HCaP\/MC. These hydrogels significantly enhanced MC3T3-E1 cell migration and proliferation in a dose- and time-dependent manner, achieving complete cell migration within 48 h. In addition, they significantly promoted alkaline phosphatase activity, collagen content, and mineralization in MC3T3-E1 cells, indicating their potential for osteogenesis. Among the hydrogel formulations, the VM-HSF\/HCaP\/MC hydrogel, with high SF and CaP content, demonstrated superior potential in promoting the osteogenic differentiation of MC3T3-E1 cells. It exhibited the highest ALP activity (11.13 \u00b1 0.91 U\/mg protein) over 14 days, along with increased collagen content (54.00 \u00b1 1.71 \u00b5g\/mg protein) and mineralization (15.79 \u00b1 1.48 mM) over 35 days. Therefore, this formulation showed a promising candidate for clinical application in localized bone regeneration, particularly in treating osteomyelitis.<\/jats:p>","DOI":"10.3390\/gels10110695","type":"journal-article","created":{"date-parts":[[2024,10,25]],"date-time":"2024-10-25T10:50:08Z","timestamp":1729853408000},"page":"695","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Vancomycin-Loaded Silk Fibroin\/Calcium Phosphate\/Methylcellulose-Based In Situ Thermosensitive Hydrogel: A Potential Function for Bone Regeneration"],"prefix":"10.3390","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6904-8497","authenticated-orcid":false,"given":"Premchirakorn","family":"Phewchan","sequence":"first","affiliation":[{"name":"Department of Pharmaceutical Technology, Faculty of Pharmaceutical Sciences and Center of Excellence for Innovation in Chemistry, Naresuan University, Phitsanulok 65000, Thailand"},{"name":"Center of Excellence for Innovation in Chemistry (PERCH-CIC), Department of Chemistry, Faculty of Science, Mahidol University, Bangkok 10400, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Artit","family":"Laoruengthana","sequence":"additional","affiliation":[{"name":"Department of Orthopedics, Faculty of Medicine, Naresuan University, Phitsanulok 65000, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3627-1086","authenticated-orcid":false,"given":"Pratthana","family":"Chomchalao","sequence":"additional","affiliation":[{"name":"College of Medicine and Public Health, Ubon Ratchathani University, Ubon Ratchathani 34190, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Supaporn","family":"Lamlertthon","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Parasitology, Faculty of Medical Sciences, Naresuan University, Phitsanulok 65000, Thailand"},{"name":"The Center of Excellence in Medical Biotechnology, Naresuan University, Phitsanulok 65000, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8570-0465","authenticated-orcid":false,"given":"Waree","family":"Tiyaboonchai","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Technology, Faculty of Pharmaceutical Sciences and Center of Excellence for Innovation in Chemistry, Naresuan University, Phitsanulok 65000, Thailand"},{"name":"Center of Excellence for Innovation in Chemistry (PERCH-CIC), Department of Chemistry, Faculty of Science, Mahidol University, Bangkok 10400, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,10,25]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1016\/j.jgar.2021.03.030","article-title":"Staphylococcus aureus clones causing osteomyelitis: A literature review (2000\u20132020)","volume":"26","author":"Monaco","year":"2021","journal-title":"J. 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