{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,8]],"date-time":"2026-04-08T01:56:59Z","timestamp":1775613419551,"version":"3.50.1"},"reference-count":48,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2025,4,1]],"date-time":"2025-04-01T00:00:00Z","timestamp":1743465600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Universidad Catolica de Cuenca, Azuay, Ecuador","award":["PICIV18-05"],"award-info":[{"award-number":["PICIV18-05"]}]},{"name":"Universidad Catolica de Cuenca, Azuay, Ecuador","award":["200795"],"award-info":[{"award-number":["200795"]}]},{"name":"Universidad Peruana Cayetano Heredia, Lima, Peru","award":["PICIV18-05"],"award-info":[{"award-number":["PICIV18-05"]}]},{"name":"Universidad Peruana Cayetano Heredia, Lima, Peru","award":["200795"],"award-info":[{"award-number":["200795"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Gels"],"abstract":"<jats:p>The study investigated the effectiveness of a babaco-based gel derived from an endemic Ecuadorian fruit for chemomechanical caries removal compared to the conventional non-rotary mechanical method. Babaco contains proteolytic enzymes that soften decayed dental tissue, making it a potential alternative for caries treatment. An ex vivo experimental study was carried out using sixty extracted human teeth, each divided into two sections: one treated with babaco gel and the other with a spoon excavator. Four extraction methods (maceration, distillation, Soxhlet, and percolation) were used to prepare the gel. After rehydration and submersion in nitric acid, an oral pathologist evaluated the efficacy of caries removal. Results showed a 78% success rate for the babaco-treated sections, significantly higher than the 14% success rate for the conventional method. The distillation-extracted gel subgroup performed best at p &lt; 0.01 Fisher Chi2. The study concluded that babaco gel is more effective for caries removal than traditional methods, highlighting its potential as a chemical-mechanical alternative.<\/jats:p>","DOI":"10.3390\/gels11040257","type":"journal-article","created":{"date-parts":[[2025,4,1]],"date-time":"2025-04-01T11:08:40Z","timestamp":1743505720000},"page":"257","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Histological Evaluation of Chemo Mechanical Caries Removal with a Babaco-Based Formulation Gel"],"prefix":"10.3390","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8952-7161","authenticated-orcid":false,"given":"Mar\u00eda del Carmen","family":"Pariona-Minaya","sequence":"first","affiliation":[{"name":"Faculty of Dentistry, University Peruana Cayetano Heredia, Lima 15102, Peru"},{"name":"Health and Wellness Department, Faculty of Dentistry, Catholic University of Cuenca, Cuenca 010107, Ecuador"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-5839-8004","authenticated-orcid":false,"given":"Melissa","family":"Berrezueta-P\u00e9rez","sequence":"additional","affiliation":[{"name":"Health and Wellness Department, Faculty of Dentistry, Catholic University of Cuenca, Cuenca 010107, Ecuador"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8216-2841","authenticated-orcid":false,"given":"Gerson","family":"Cabezas-Bernhardt","sequence":"additional","affiliation":[{"name":"PhD. Program Alumnni, Faculty of Dentistry, University Peruana Cayetano Heredia, Lima 15102, Peru"},{"name":"Health Science Department, Faculty of Dentistry, University San Francisco de Quito, Quito 170901, Ecuador"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4411-4221","authenticated-orcid":false,"given":"Ebingen","family":"Villavicencio-Caparo","sequence":"additional","affiliation":[{"name":"Faculty of Dentistry, University Peruana Cayetano Heredia, Lima 15102, Peru"},{"name":"Research Group, Health and Wellness Department, Faculty of Dentistry, Catholic University of Cuenca, Cuenca 010107, Ecuador"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2025,4,1]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Santos, T.M.L., Bresciani, E., de Matos, F.S., Camargo, S.E.A., Hidalgo, A.P.T., Rivera, L.M.L., de Bernardino, \u00cd.M., and Paranhos, L.R. (2020). Comparison between conventional and chemomechanical approaches for the removal of carious dentin: An in vitro study. Sci. Rep., 10.","DOI":"10.1038\/s41598-020-65159-x"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"233","DOI":"10.1016\/j.sdentj.2023.01.010","article-title":"Chemomechanical caries removal methods: A literature review","volume":"35","author":"Maashi","year":"2023","journal-title":"Saudi. Dent. J."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"332","DOI":"10.12688\/f1000research.131919.1","article-title":"A comparative evaluation of micro shear bond strength and microleakage between the resin-modified glass ionomer cement and residual dentin following excavation of carious dentin using Carie CareTM and conventional caries removal in primary teeth: An in vitro study","volume":"12","author":"Nair","year":"2023","journal-title":"F1000Research"},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Silva, Z.S., Huang, Y.Y., de Freitas, L.F., Fran\u00e7a, C.M., Botta, S.B., Ana, P.A., Mesquita-Ferrari, R.A., Fernandes, K.P.S., Deana, A., and Leal, C.R.L. (2016). Papain gel containing methylene blue for simultaneous caries removal and antimicrobial photoinactivation against Streptococcus mutans biofilms. Sci. Rep., 6.","DOI":"10.1038\/srep33270"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"277","DOI":"10.17245\/jdapm.2018.18.5.277","article-title":"New treatment method for pain and reduction of local anesthesia use in deep caries","volume":"18","author":"Yun","year":"2018","journal-title":"J. Dent. Anesthesia Pain Med."},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Bratu, D.C., Nikolajevic-Stoican, N., Popa, G., Pop, S.I., Drago\u0219, B., and Luca, M.M. (2022). A Bibliometric Analysis (2010\u20132020) of the Dental Scientific Literature on Chemomechanical Methods of Caries Removal Using Carisolv and BRIX3000. Medicina, 58.","DOI":"10.3390\/medicina58060788"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"289","DOI":"10.17796\/1053-4628-41.4.289","article-title":"SEM Analysis of Residual Dentin Surface in Primary Teeth Using Different Chemomechanical Caries Removal Agents","volume":"41","author":"Thakur","year":"2017","journal-title":"J. Clin. Pediatr. Dent."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"103280","DOI":"10.1016\/j.jdent.2020.103280","article-title":"Comparing the efficacies of two chemomechanical caries removal agents (2.25% sodium hypochlorite gel and brix 3000), in caries removal and patient cooperation: A randomized controlled clinical trial","volume":"93","author":"Alkhouli","year":"2020","journal-title":"J. Dent."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"961","DOI":"10.1007\/s10658-020-02037-2","article-title":"Exploring the virome of Vasconcellea x heilbornii: The first step towards a sustainable production program for babaco in Ecuador","volume":"157","author":"Flores","year":"2020","journal-title":"Eur. J. Plant Pathol."},{"key":"ref_10","first-page":"480","article-title":"Elaboraci\u00f3n de un removedor de caries en base a babaco","volume":"63","year":"2022","journal-title":"Investig. Clin."},{"key":"ref_11","first-page":"37","article-title":"Universidad de las Fuerzas Armadas-ESPE.; Av. General Rumi\u00f1ahui s\/n, Sangolqu\u00ed- Ecuador, P.O.Box:171-5-231B. Propagation methods in Babaco plants (Vasconcella x helbornii)","volume":"6","year":"2019","journal-title":"Trop. Plant Res."},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Mosquera-Yuqui, F., Flores, F.J., Moncayo, E.A., Garz\u00f3n-Proa\u00f1o, B.A., M\u00e9ndez, M.A., Guevara, F.E., Quito-Avila, D.F., Viera, W., Cornejo-Franco, J.F., and Izquierdo, A.R. (2022). Phylodynamics and Coat Protein Analysis of Babaco Mosaic Virus in Ecuador. Plants, 11.","DOI":"10.3390\/plants11131646"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"E167","DOI":"10.2341\/14-021-LIT","article-title":"Systematic Review and Meta-Analysis of Randomized Clinical Trials on Chemomechanical Caries Removal","volume":"40","author":"Hamama","year":"2015","journal-title":"Oper. Dent."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"462","DOI":"10.2174\/1874210601509010462","article-title":"Caries Removal by Chemomechanical (CarisolvTM) vs. Rotary Drill: A Systematic Review","volume":"9","author":"Maru","year":"2015","journal-title":"Open Dent. J."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"569","DOI":"10.3109\/00016357.2015.1023353","article-title":"Comparison of Carisolv system vs traditional rotating instruments for caries removal in the primary dentition: A systematic review and meta-analysis","volume":"73","author":"Lai","year":"2015","journal-title":"Acta Odont. Scandinavica"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"432","DOI":"10.1111\/joor.12161","article-title":"How to make the choice of the carious removal methods, Carisolv or traditional drilling? A meta-analysis","volume":"41","author":"Li","year":"2014","journal-title":"J. Oral Rehabil."},{"key":"ref_17","first-page":"1","article-title":"ART with or without the aid of chemomechanical agents: A systematic review","volume":"28","author":"Magno","year":"2024","journal-title":"Clin. Oral Investig."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"2","DOI":"10.4103\/JCD.JCD_263_19","article-title":"Systematic review on alternative methods for caries removal in permanent teeth","volume":"23","author":"Senthilkumar","year":"2020","journal-title":"J. Conserv. Dent."},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"de Ferreira, L.A.Q., Diniz, I.M.A., da Peixoto, R.T.R.C., Gomes, N.A., de Caneschi, C.S., Spineli, L.M., and Martins, C.C. (2023). Efficacy of antiseptics and chemomechanical methods for dentin caries lesions: A systematic review with GRADE approach. Front. Oral Health, 4.","DOI":"10.3389\/froh.2023.1110634"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"257","DOI":"10.4103\/jisppd.jisppd_308_24","article-title":"Comparison of smart burs and chemomechanical caries removal systems in primary molars\u2014A systematic review and meta-analysis","volume":"42","author":"Mehrotra","year":"2024","journal-title":"J. Indian Soc. Pedod. Prev. Dent."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"6457","DOI":"10.1007\/s00784-022-04695-4","article-title":"Influence of the chemomechanical and mechanical carious tissue removal on the risk of restorative failure: A systematic review and meta-analysis","volume":"26","author":"Rossoni","year":"2022","journal-title":"Clin. Oral Investig."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.jdent.2014.10.004","article-title":"Effects of using different criteria for caries removal: A systematic review and network meta-analysis","volume":"43","author":"Schwendicke","year":"2015","journal-title":"J. Dent."},{"key":"ref_23","unstructured":"Acosta, R. (2011). Estudio de la Variaci\u00f3n de la Actividad Enzim\u00e1tica Proteol\u00edtica del L\u00e1tex del Babaco (Vasconcellea heilbornii cv babaco) en Funci\u00f3n de la Edad del Fruto\u201d. [Bachelor\u2019s Thesis, Escuela Politecnica Nacional]."},{"key":"ref_24","unstructured":"Reyes, F. (2007). Determination of Glycosidades in Babaco Purification and Characterization of a-Mannodidase from Babaco Latex, Escuela Politecnica Nacional."},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"Cuenca-Le\u00f3n, K., Pacheco-Quito, E.M., Granda-Granda, Y., V\u00e9lez-Le\u00f3n, E., and Zarzuelo-Casta\u00f1eda, A. (2022). Phytotherapy: A Solution to Decrease Antifungal Resistance in the Dental Field. Biomolecules, 12.","DOI":"10.3390\/biom12060789"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"295","DOI":"10.2147\/CCIDE.S478181","article-title":"Effectiveness of Lemon Verbena (Cymbopogon citratus) in Oral Candidiasis: A Systematic Review","volume":"16","year":"2024","journal-title":"Clin. Cosmet. Investig. Dent."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"e826","DOI":"10.4317\/jced.58208","article-title":"Cytotoxicity and dentin composition alterations promoted by different chemomechanical caries removal agents: A preliminary study","volume":"13","author":"Guedes","year":"2021","journal-title":"J. Clin. Exp. Dent."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"767","DOI":"10.1007\/s10103-019-02857-7","article-title":"The effects of photodynamic therapy with blue light and papain-based gel associated with Urucum, on collagen and fibroblasts: A spectroscopic and cytotoxicity analysis","volume":"35","author":"Silva","year":"2020","journal-title":"Lasers Med. Sci."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"41","DOI":"10.4103\/sjmms.sjmms_104_18","article-title":"Efficacy and Efficiency of Papacarie versus Conventional Method in Caries Removal in Primary Teeth: An SEM Study","volume":"8","author":"AlHumaid","year":"2020","journal-title":"Saudi J. Med. Med. Sci."},{"key":"ref_30","first-page":"e34432","article-title":"Evaluation of the Efficacy of Recent Caries Removal Techniques: An In Vitro Study","volume":"15","author":"Sharma","year":"2023","journal-title":"Cureus"},{"key":"ref_31","doi-asserted-by":"crossref","unstructured":"Saber, A.M., Altoukhi, D.H., Horaib, M.F., El-Housseiny, A.A., Alamoudi, N.M., and Sabbagh, H.J. (2018). Consequences of early extraction of compromised first permanent molar: A systematic review. BMC Oral Health, 18.","DOI":"10.1186\/s12903-018-0516-4"},{"key":"ref_32","doi-asserted-by":"crossref","unstructured":"Cardoso, M., Coelho, A., Lima, R., Amaro, I., Paula, A., Marto, C.M., Sousa, J., Spagnuolo, G., Ferreira, M.M., and Carrilho, E. (2020). Efficacy and Patient\u2019s Acceptance of Alternative Methods for Caries Removal-a Systematic Review. J. Clin. Med., 9.","DOI":"10.3390\/jcm9113407"},{"key":"ref_33","first-page":"383","article-title":"Ansiedad, miedo y comportamiento en odontopediatria utilizando Brix 3000 y m\u00e9todo rotatorio para remoci\u00f3n de caries","volume":"16","author":"Sierra","year":"2019","journal-title":"Duazary: Rev. Int. Cien. Salud."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"869","DOI":"10.1007\/s40368-022-00726-6","article-title":"Worldwide research trends on using chemical-mechanical caries removal products over the years: A critical review","volume":"23","author":"Souza","year":"2022","journal-title":"Eur. Arch. Paediatr. Dent."},{"key":"ref_35","doi-asserted-by":"crossref","unstructured":"Bottega, F., Bussadori, S.K., Battisti, I.D.E., Vieira, E.P., Pompeo, T.S., and Winkelmann, E.R. (2018). Costs and benefits of Papacarie in pediatric dentistry: A randomized clinical trial. Sci. Rep., 8.","DOI":"10.1038\/s41598-018-36092-x"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"161","DOI":"10.5005\/jp-journals-10005-1504","article-title":"Effect of a Papain-based Chemomechanical Agent on Structure of Dentin and Bond Strength: An Study","volume":"11","author":"Nair","year":"2018","journal-title":"Int. J. Clin. Pediatr. Dent."},{"key":"ref_37","doi-asserted-by":"crossref","unstructured":"Ghanem, A.Y., Talaat, D.M., Essawy, M.M., and Bakry, N. (2023). The effectiveness of Carie-CareTM; chemomechanical caries removal technique in primary teeth: Randomized controlled clinical trial. BMC Oral Health, 23.","DOI":"10.1186\/s12903-023-03594-8"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"361","DOI":"10.1111\/ipd.12364","article-title":"Effects of Papacarie on children with dental caries in primary teeth: A systematic review and meta-analysis","volume":"28","author":"Deng","year":"2018","journal-title":"Int. J. Paediatr. Dent."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1007\/s40368-014-0140-1","article-title":"A comparative clinical study of various methods of caries removal in children","volume":"16","author":"Soni","year":"2015","journal-title":"Eur. Arch. Paediatr. Dent."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"76","DOI":"10.22201\/fq.18708404e.1996.2.66669","article-title":"Historia de la Destilaci\u00f3n. de Facultad","volume":"7","author":"Valiente","year":"2018","journal-title":"Educ. Qu\u00edmica"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"e17356","DOI":"10.1016\/j.heliyon.2023.e17356","article-title":"Green synthesis of silver nanoparticles using Vernonia amygdalina plant extract and its antimicrobial activities","volume":"9","author":"Tesfaye","year":"2023","journal-title":"Heliyon"},{"key":"ref_42","first-page":"1","article-title":"Automatizaci\u00f3n de la destilaci\u00f3n de alcohol de la UEB destiler\u00eda de la ronera Santiago de Cuba","volume":"33","year":"2013","journal-title":"Tecnol. Qu\u00edmica"},{"key":"ref_43","first-page":"14","article-title":"Comparaci\u00f3n de los m\u00e9todos de extracci\u00f3n para hidrocarburos arom\u00e1ticos polic\u00edclicos en sedimentos marinos empleando soxhlet y ba\u00f1o ultras\u00f3nico","volume":"47","author":"Viera","year":"2020","journal-title":"Rev. Cent. Az\u00facar"},{"key":"ref_44","doi-asserted-by":"crossref","unstructured":"Wirth, T. (2017). Organic synthesis in flow for medicinal chemistry. Bioorg. Med. Chem., 25.","DOI":"10.1016\/j.bmc.2017.11.013"},{"key":"ref_45","first-page":"196","article-title":"A review on the extraction methods use in medicinal plants, principle, strength, and limitation","volume":"4","author":"Azwanida","year":"2015","journal-title":"Med. Aromat. Plants"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"331","DOI":"10.1016\/j.supflu.2017.03.023","article-title":"Application of conventional and non-conventional extraction approaches for extraction of Erica carnea L.: Chemical profile and biological activity of obtainde extracts","volume":"128","author":"Velickovic","year":"2017","journal-title":"J. Supercrit. Fluids"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"200","DOI":"10.1038\/s41573-020-00114-z","article-title":"International Natural Product Sciences Taskforce, Natural products in drug discovery: Advances and opportunities","volume":"20","author":"Atanasov","year":"2021","journal-title":"Nat. Rev. Drug Discov."},{"key":"ref_48","first-page":"115","article-title":"Concept of standardization, extraction, and pre phytochemical screening strategies for herbal drug","volume":"2","author":"Pandey","year":"2014","journal-title":"J. Pharmacogn. Phytochem."}],"container-title":["Gels"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2310-2861\/11\/4\/257\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T17:07:21Z","timestamp":1760029641000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2310-2861\/11\/4\/257"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,4,1]]},"references-count":48,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2025,4]]}},"alternative-id":["gels11040257"],"URL":"https:\/\/doi.org\/10.3390\/gels11040257","relation":{},"ISSN":["2310-2861"],"issn-type":[{"value":"2310-2861","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,4,1]]}}}