{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T00:45:57Z","timestamp":1788828357177,"version":"build-2803163510"},"reference-count":63,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2025,9,23]],"date-time":"2025-09-23T00:00:00Z","timestamp":1758585600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Natural Science Foundation of China","award":["82173731"],"award-info":[{"award-number":["82173731"]}]},{"name":"National Natural Science Foundation of China","award":["17490742500"],"award-info":[{"award-number":["17490742500"]}]},{"name":"Scientific Research Project of Shanghai International Science and Technology Cooperation Fund Project","award":["82173731"],"award-info":[{"award-number":["82173731"]}]},{"name":"Scientific Research Project of Shanghai International Science and Technology Cooperation Fund Project","award":["17490742500"],"award-info":[{"award-number":["17490742500"]}]},{"name":"Shanghai Frontiers Research Center of the Hadal Biosphere","award":["82173731"],"award-info":[{"award-number":["82173731"]}]},{"name":"Shanghai Frontiers Research Center of the Hadal Biosphere","award":["17490742500"],"award-info":[{"award-number":["17490742500"]}]},{"name":"CSPFTZ Lingang Special Area Marine Biomedical Innovation Platform, Shanghai, China","award":["82173731"],"award-info":[{"award-number":["82173731"]}]},{"name":"CSPFTZ Lingang Special Area Marine Biomedical Innovation Platform, Shanghai, China","award":["17490742500"],"award-info":[{"award-number":["17490742500"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Gels"],"abstract":"<jats:p>Carboxymethyl chitosan (CMC)-based hydrogels have emerged as promising candidates for wound dressing applications due to their excellent biocompatibility and tunable physicochemical properties. In this study, a novel hydrogel functionalized with hyaluronic acid (HA) and RGD peptides (RGD) was fabricated and evaluated for its structural characteristics and wound-healing potential. Using CMC as the base matrix and EDC\/NHS as crosslinking agents, four hydrogel variants were fabricated: CMC gel, CMC-HA gel, CMC-RGD gel, and CMC-HA-RGD gel. The preliminary cell compatibility experiment identified the optimal formulation as 1% CMC, 0.9% HA, and 0.02 mg\/mL RGD, crosslinked with 1 vol% EDC and 0.05 wt% NHS. Scanning electron microscopy showed a porous architecture (100\u2013400 \u03bcm), conducive to fibroblast viability and proliferation. Zeta potential measurements (|\u03b6| &gt; 30 mV) indicated colloidal stability of the hydrogel system. Fourier-transform infrared spectroscopy confirmed successful crosslinking and integration of HA and RGD via hydrogen bonding and electrostatic interactions, forming a stable three-dimensional network. Thermogravimetric analysis revealed enhanced thermal stability upon HA\/RGD incorporation. CCK-8 assays demonstrated significantly improved cell viability with HA\/RGD loading (p &lt; 0.05), while Ki-67 immunofluorescence confirmed enhanced fibroblast proliferation, with the CMC-HA-RGD gel showing the most pronounced effect. In vitro scratch assay results demonstrated that the CMC-HA-RGD hydrogel dressing significantly enhanced cellular migration compared to other carboxymethyl chitosan-based hydrogel groups (p &lt; 0.05). The observed statistically significant improvement in cell migration rate versus controls underscores the distinctive enhancement of synergistic HA and RGD modification in accelerating cellular migration and facilitating wound repair. Collectively, these findings suggest that the CMC-HA-RGD hydrogel possesses favorable physicochemical and biological properties and holds strong potential as an advanced wound dressing for the treatment of chronic and refractory wounds.<\/jats:p>","DOI":"10.3390\/gels11100765","type":"journal-article","created":{"date-parts":[[2025,9,23]],"date-time":"2025-09-23T14:32:53Z","timestamp":1758637973000},"page":"765","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Biofunctional Carboxymethyl Chitosan Hydrogel Incorporating Hyaluronic Acid and RGD Peptides for Accelerated Wound Repair"],"prefix":"10.3390","volume":"11","author":[{"given":"Shuyue","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Marine Pharmacology, College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qing","family":"Yang","sequence":"additional","affiliation":[{"name":"Shanghai Knowhub Technology Co., Ltd., Pudong New Area, Shanghai 201210, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiren","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of Marine Pharmacology, College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-6050-0688","authenticated-orcid":false,"given":"Youshiqi","family":"Zhou","sequence":"additional","affiliation":[{"name":"Department of Marine Pharmacology, College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1375-2514","authenticated-orcid":false,"given":"Xiaoqing","family":"Tian","sequence":"additional","affiliation":[{"name":"Department of Marine Pharmacology, College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China"},{"name":"Key Laboratory of Oceanic and Polar Fisheries, Ministry of Agriculture and Rural Affairs, East China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Shanghai 200090, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1710-8582","authenticated-orcid":false,"given":"Wenhui","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Marine Pharmacology, College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China"},{"name":"Marine Biomedical Science and Technology Innovation Platform of Lin-Gang Special Area, Shanghai 201306, China"},{"name":"Putuo Branch of International Combined Research Center for Marine Biological Sciences, Zhoushan 316104, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4115-9918","authenticated-orcid":false,"given":"Jeevithan","family":"Elango","sequence":"additional","affiliation":[{"name":"Department of Biomaterials Engineering, Faculty of Health Sciences, UCAM\u2014Universidad Cat\u00f3lica San Antonio de Murcia, Campus de los Jer\u00f3nimos 135, 30107 Guadalupe, Murcia, Spain"},{"name":"Center of Molecular Medicine and Diagnostics (COMManD), Department of Biochemistry, Saveetha Institute of Medical and Technical Sciences, Saveetha Dental College and Hospitals, Saveetha University, Chennai 600077, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1805-5408","authenticated-orcid":false,"given":"Xiaozhen","family":"Diao","sequence":"additional","affiliation":[{"name":"Department of Marine Pharmacology, College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China"},{"name":"Marine Biomedical Science and Technology Innovation Platform of Lin-Gang Special Area, Shanghai 201306, China"},{"name":"Putuo Branch of International Combined Research Center for Marine Biological Sciences, Zhoushan 316104, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,9,23]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"208","DOI":"10.1089\/wound.2013.0506","article-title":"Traditional Therapies for Skin Wound Healing","volume":"5","author":"Pereira","year":"2016","journal-title":"Adv. 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