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Chlorhexidine was incorporated into the hydrogels to provide antibacterial properties. The concentration of chlorhexidine ranged from 0.1 to 0.8 wt%. The prepared hydrogels showed better early onset cytocompatibility than the cell culture dish used as a control. For the evaluation of antibacterial properties, strains of Streptococcus mutans, Streptococcus sanguis, and Streptococcus anginosus were used. The results indicated antibacterial activity at all chlorhexidine concentrations tested, with the area of bacterial inhibition increasing with increasing bactericidal content in the hydrogel films. Adding bactericide into cellulose films did not compromise their early onset cytocompatibility in the first 72 h. The study suggests that adding chlorhexidine provides the hydrogel films with antibacterial properties, potentially expanding their applications in dentistry.<\/jats:p>","DOI":"10.3390\/gels11110891","type":"journal-article","created":{"date-parts":[[2025,11,5]],"date-time":"2025-11-05T17:06:06Z","timestamp":1762362366000},"page":"891","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Cellulose Hydrogels Derived from Pineapple Bagasse for Potential Dental Applications: Chlorhexidine-Loaded Hydrogels with Antibacterial and Cytocompatible Properties"],"prefix":"10.3390","volume":"11","author":[{"given":"Itzel","family":"Nevarez-Rico","sequence":"first","affiliation":[{"name":"Instituto de Ciencias Biom\u00e9dicas, Universidad Aut\u00f3noma de Cd. Ju\u00e1rez, Av. 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