{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,26]],"date-time":"2026-02-26T11:07:08Z","timestamp":1772104028157,"version":"3.50.1"},"reference-count":115,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2026,2,26]],"date-time":"2026-02-26T00:00:00Z","timestamp":1772064000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100002858","name":"China Postdoctoral Science Foundation","doi-asserted-by":"publisher","award":["2022M711087"],"award-info":[{"award-number":["2022M711087"]}],"id":[{"id":"10.13039\/501100002858","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Heilongjiang Postdoctoral Fund","award":["LBH-Z21194"],"award-info":[{"award-number":["LBH-Z21194"]}]},{"name":"Heilongjiang Postdoctoral Fund","award":["LBH-Z21193"],"award-info":[{"award-number":["LBH-Z21193"]}]},{"name":"Research Initiation Plan for Academically Accomplished and Introduced Talents at Heilongjiang Bayi Agricultural University","award":["XYB202020"],"award-info":[{"award-number":["XYB202020"]}]},{"name":"Research Initiation Plan for Academically Accomplished and Introduced Talents at Heilongjiang Bayi Agricultural University","award":["XYB202019"],"award-info":[{"award-number":["XYB202019"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Gels"],"abstract":"<jats:p>Thermosensitive hydrogels have emerged as promising intelligent biomaterials for minimally invasive delivery and targeted therapy. Chitosan\/gelatin thermosensitive hydrogels, integrating the biocompatibility, biodegradability, and antibacterial activity of chitosan with the excellent adhesive properties of gelatin, exhibit unique injectability, temperature-responsive gelation, and tunable physicochemical properties. This review systematically summarizes the key performance parameters of chitosan\/gelatin thermosensitive hydrogels, including injectability, gelation characteristics (with sol-gel transition tunable between 37 and 42 \u00b0C to match diverse species\u2019 body temperatures), mechanical properties, biocompatibility, degradation behavior (tunable from 1 to 8 weeks), drug-loading\/release capabilities, and multi-stimuli responsiveness (pH\/ROS\/enzyme). It focuses on exploring their feasibility and suitability as acupoint embedding materials in Traditional Chinese Veterinary Medicine (TCVM), addressing the technical bottlenecks of traditional acupoint catgut embedding (e.g., unstable degradation, insufficient biocompatibility, and lack of drug-loading capacity). While recent studies have demonstrated the utility of such hydrogels in human disease models (e.g., rheumatoid arthritis and Parkinson\u2019s disease), their translation to veterinary acupoint therapy remains largely unexplored. The prospective application of these hydrogels in treating common animal diseases (e.g., piglet diarrhea, canine degenerative joint disease, and equine laminitis) is, therefore, proposed and analyzed as an illustrative paradigm, emphasizing its integrated \u201cstimulation\u2013drug delivery\u201d function and cross-species adaptability. Additionally, the current challenges (e.g., animal-specific formulation optimization, unclear mechanism of action, and insufficient long-term safety data) and future research directions (e.g., veterinary-specific formulation development, mechanistic exploration, and clinical translation) are highlighted. This review aims to promote the interdisciplinary integration of TCVM and smart biomaterials, provide precision strategies for animal disease treatment, and ultimately contribute to the modernization and standardization of TCVM technologies.<\/jats:p>","DOI":"10.3390\/gels12030193","type":"journal-article","created":{"date-parts":[[2026,2,26]],"date-time":"2026-02-26T09:31:32Z","timestamp":1772098292000},"page":"193","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Thermosensitive Chitosan\/Gelatin Hydrogels in Traditional Chinese Veterinary Medicine: A Prospective Review on Modernizing Acupoint Embedding"],"prefix":"10.3390","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5432-6650","authenticated-orcid":false,"given":"Yingying","family":"Xie","sequence":"first","affiliation":[{"name":"College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing 163319, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7031-4484","authenticated-orcid":false,"given":"Xuequan","family":"Hu","sequence":"additional","affiliation":[{"name":"College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing 163319, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ying","family":"Li","sequence":"additional","affiliation":[{"name":"College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing 163319, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jianfa","family":"Wang","sequence":"additional","affiliation":[{"name":"College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing 163319, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9170-0517","authenticated-orcid":false,"given":"Rui","family":"Wu","sequence":"additional","affiliation":[{"name":"College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing 163319, China"},{"name":"College of Biology and Agriculture, Jiamusi University, Jiamusi 154007, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2026,2,26]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"118192","DOI":"10.1016\/j.carbpol.2021.118192","article-title":"Prospects and challenges of anticancer agents\u2019 delivery via chitosan-based drug carriers to combat breast cancer: A review","volume":"268","author":"Wang","year":"2021","journal-title":"Carbohydr. Polym."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"460","DOI":"10.1177\/0885328221995425","article-title":"Biological and mechanical evaluation of mineralized-hydrogel scaffolds for tissue engineering applications","volume":"36","author":"Ritchie","year":"2021","journal-title":"J. Biomater. Appl."},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Yarahmadi, A., Dousti, B., Karami-Khorramabadi, M., and Afkhami, H. (2024). Materials based on biodegradable polymers chitosan\/gelatin: A review of potential applications. Front. Bioeng. Biotechnol., 12.","DOI":"10.3389\/fbioe.2024.1397668"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"114451","DOI":"10.1016\/j.jconrel.2025.114451","article-title":"Bindarit-loaded hydrogel sustained-release system alleviates periodontitis in mice via CC motif chemokine ligand 2-dependent monocyte chemotaxis","volume":"389","author":"Jiang","year":"2026","journal-title":"J. Control. Release"},{"key":"ref_5","first-page":"252","article-title":"In vitro study of bone morphogenetic protein 2 gelatin\/chitosan hydrogel sustained-release system composite hydroxyapatite\/zirconium dioxide foam ceramics and induced pluripotent stem cells derived mesenchymal stem cells","volume":"33","author":"Chai","year":"2019","journal-title":"Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"e38314","DOI":"10.1097\/MD.0000000000038314","article-title":"A comprehensive practical review of acupoint embedding as a semi-permanent acupuncture: A mini review","volume":"103","author":"Kazemi","year":"2024","journal-title":"Medicine"},{"key":"ref_7","first-page":"e68071","article-title":"An Acupoint Catgut-embedding Therapy for Treating Obesity","volume":"218","author":"Tang","year":"2025","journal-title":"J. Vis. Exp."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"409","DOI":"10.1186\/s12951-021-01157-z","article-title":"Acupoint nanocomposite hydrogel for simulation of acupuncture and targeted delivery of triptolide against rheumatoid arthritis","volume":"19","author":"Ren","year":"2021","journal-title":"J. Nanobiotechnol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"708","DOI":"10.1039\/D3NH00469D","article-title":"Use of nano-enabled approaches to advance acupuncture therapy for disease management","volume":"9","author":"Xu","year":"2024","journal-title":"Nanoscale Horiz."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"e2301933","DOI":"10.1002\/adhm.202301933","article-title":"Transdermal Sustained Release Properties and Anti-Photoaging Efficacy of Liposome-Thermosensitive Hydrogel System","volume":"13","author":"Wu","year":"2024","journal-title":"Adv. Healthc. Mater."},{"key":"ref_11","first-page":"102268","article-title":"Development of injectable cuttlebone derived nanohydroxyapatite hydrogel for osteoblast cell encapsulation","volume":"44","author":"Arpornmaeklong","year":"2025","journal-title":"Biochem. Biophys. Rep."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"131337","DOI":"10.1016\/j.ijbiomac.2024.131337","article-title":"Injectable, self-healing hydrogels based on gelatin, quaternized chitosan, and laponite as localized celecoxib delivery system for nucleus pulpous repair","volume":"266","author":"Nezadi","year":"2024","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"115089","DOI":"10.1016\/j.colsurfb.2025.115089","article-title":"Injectable chitosan-based thermosensitive hydrogel loaded with adipose-derived mesenchymal stem cells promotes pressure ulcer healing","volume":"256","author":"He","year":"2025","journal-title":"Colloids Surf. B Biointerfaces"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"119769","DOI":"10.1016\/j.carbpol.2022.119769","article-title":"Thermosensitive injectable hydrogel based on chitosan-polygalacturonic acid polyelectrolyte complexes for bone tissue engineering","volume":"294","author":"Wasupalli","year":"2022","journal-title":"Carbohydr. Polym."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"74","DOI":"10.1007\/s42995-021-00116-9","article-title":"Design and evaluation of chitosan-amino acid thermosensitive hydrogel","volume":"4","author":"Tong","year":"2022","journal-title":"Mar. Life Sci. Technol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"7492","DOI":"10.1039\/D1BM01129D","article-title":"Thermosensitive chitosan-based hydrogels supporting motor neuron-like NSC-34 cell differentiation","volume":"9","author":"Stanzione","year":"2021","journal-title":"Biomater. Sci."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"145434","DOI":"10.1016\/j.ijbiomac.2025.145434","article-title":"Design and properties of cyclodextrin grafted chitosan\/sodium alginate dual-network hydrogel based on physical cross-linking","volume":"319","author":"Jiang","year":"2025","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"3732","DOI":"10.1021\/acs.biomac.5c00304","article-title":"Chitosan-Based Thermal-Coagulation Hydrogel System Driven by Multiple Interactions: Oxidation-Induced Fast Gelation and Enhanced Performance","volume":"26","author":"Liu","year":"2025","journal-title":"Biomacromolecules"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"113193","DOI":"10.1016\/j.colsurfb.2023.113193","article-title":"Optimization of thermoresponsive chitosan\/beta-glycerophosphate hydrogels for injectable neural tissue engineering application","volume":"224","author":"Bhuiyan","year":"2023","journal-title":"Colloids Surf. B Biointerfaces"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"130266","DOI":"10.1016\/j.ijbiomac.2024.130266","article-title":"Gelatin\/O-carboxymethyl chitosan injectable self-healing hydrogels for ibuprofen and naproxen dual release","volume":"263","author":"Mohseni","year":"2024","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"147268","DOI":"10.1016\/j.ijbiomac.2025.147268","article-title":"Antioxidant and biocompatible hydrogel based on genipin-crosslinked quaternized chitosan and tannic acid for skin repair","volume":"327","author":"Chi","year":"2025","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"113413","DOI":"10.1016\/j.colsurfb.2023.113413","article-title":"Nano-SiO2 reinforced alginate-chitosan-gelatin nanocomposite hydrogels with improved physicochemical properties and biological activity","volume":"228","author":"Chen","year":"2023","journal-title":"Colloids Surf. B Biointerfaces"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"1713","DOI":"10.1039\/D2TB02511F","article-title":"Electrospun fibrous membrane reinforced hydrogels with preferable mechanical and tribological performance as cartilage substitutes","volume":"11","author":"Chen","year":"2023","journal-title":"J. Mater. Chem. B"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"121973","DOI":"10.1016\/j.carbpol.2024.121973","article-title":"Antibacterial adhesive based on oxidized tannic acid-chitosan for rapid hemostasis","volume":"333","author":"Khosravi","year":"2024","journal-title":"Carbohydr. Polym."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"141020","DOI":"10.1016\/j.ijbiomac.2025.141020","article-title":"Blueberry leaf polysaccharide\/gelatin composite gel: Preparation, characterization, and formation mechanism","volume":"304","author":"Zhang","year":"2025","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"125640","DOI":"10.1016\/j.ijbiomac.2023.125640","article-title":"In situ forming alginate\/gelatin hybrid hydrogels containing doxorubicin loaded chitosan\/AuNPs nanogels for the local therapy of breast cancer","volume":"246","author":"Fathi","year":"2023","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"e10244","DOI":"10.1002\/btm2.10244","article-title":"Novel nanofibrous membrane-supporting stem cell sheets for plasmid delivery and cell activation to accelerate wound healing","volume":"7","author":"Zhu","year":"2022","journal-title":"Bioeng. Transl. Med."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"117370","DOI":"10.1016\/j.foodres.2025.117370","article-title":"Low-temperature enzymatic glycosylation minimizes AGEs in gelatin modification: Structure-function relationships","volume":"221","author":"Wang","year":"2025","journal-title":"Food Res. Int."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"730","DOI":"10.1016\/j.ijbiomac.2022.11.136","article-title":"Fortified gelatin-based hydrogel scaffold with simvastatin-mixed nanomicelles and platelet rich plasma as a promising bioimplant for tissue regeneration","volume":"225","author":"Yahia","year":"2023","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"111846","DOI":"10.1016\/j.msec.2020.111846","article-title":"Recombinant human collagen\/chitosan-based soft hydrogels as biomaterials for soft tissue engineering","volume":"121","author":"Yang","year":"2021","journal-title":"Mater. Sci. Eng. C Mater. Biol. Appl."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"140213","DOI":"10.1016\/j.ijbiomac.2025.140213","article-title":"Effect of nanocomposite chitosan\/hydroxyapatite pH-induced hydrogels on the osteogenic differentiation of spheroids from adipose stem cells","volume":"299","author":"Toia","year":"2025","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"148068","DOI":"10.1016\/j.ijbiomac.2025.148068","article-title":"The effect of chitosan molecular weight and its content on the mechanical, microstructural and biological parameters of biomaterial intended for cartilage tissue regeneration","volume":"331","author":"Vivcharenko","year":"2025","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"128682","DOI":"10.1016\/j.ijbiomac.2023.128682","article-title":"Physically cross-linked chitosan gel with tunable mechanics and biodegradability for tissue engineering scaffold","volume":"257","author":"Pan","year":"2024","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_34","doi-asserted-by":"crossref","unstructured":"Ciarleglio, G., Clarizia, V., Toto, E., and Santonicola, M.G. (2025). Multilayer pH-Responsive Hydrogels Fabricated via Two-Step Ionic Crosslinking: Towards Advanced Wound Dressing Materials. Gels, 11.","DOI":"10.3390\/gels11100840"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"124658","DOI":"10.1016\/j.carbpol.2025.124658","article-title":"Advances in chitosan\/gelatin scaffold design: Bridging manufacturing, crosslinking, and structure\u2014Function dynamics","volume":"373","author":"Cesquim","year":"2026","journal-title":"Carbohydr. Polym."},{"key":"ref_36","doi-asserted-by":"crossref","unstructured":"Molaei, R., Hosseinkhani, A., and Saberian, M. (2025). Molecular Regulation of Tissue Remodeling Through Chitosan-Based Hydrogels in Wound Healing Dynamics. Tissue Eng. Part B Rev.","DOI":"10.1089\/ten.teb.2025.0078"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"28","DOI":"10.1038\/s42004-023-00829-1","article-title":"Multi-responsive chitosan-based hydrogels for controlled release of vincristine","volume":"6","author":"Omrani","year":"2023","journal-title":"Commun. Chem."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"148460","DOI":"10.1016\/j.ijbiomac.2025.148460","article-title":"Synergistic effect of silver nanoparticles and titanium nanotubes loaded chitosan hydrogels for biomedical applications","volume":"331","author":"Sivabalan","year":"2025","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"101477","DOI":"10.1016\/j.mtbio.2025.101477","article-title":"Hydrogel inspired by \u201cadobe\u201d with antibacterial and antioxidant properties for diabetic wound healing","volume":"31","author":"Li","year":"2025","journal-title":"Mater. Today Bio"},{"key":"ref_40","unstructured":"(2017). Biological Evaluation of Medical Devices\u2014Part 4: Selection of Tests for Interactions with Blood (Standard No. ISO 10993-4:2017)."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"127448","DOI":"10.1016\/j.ijbiomac.2023.127448","article-title":"Synthesis and characterization of chitosan\/collagen\/polycaprolactone hydrogel films with enhanced biocompatibility and hydrophilicity for artificial tendon applications","volume":"253","author":"Jahanbakhshi","year":"2023","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"131076","DOI":"10.1016\/j.ijbiomac.2024.131076","article-title":"Chitosan thermogelation and cascade mineralization via sequential CaCO3 incorporations for wound care","volume":"266","author":"Liu","year":"2024","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"373","DOI":"10.1016\/j.ijbiomac.2022.12.037","article-title":"Hyaluronic acid and chitosan-based injectable and self-healing hydrogel with inherent antibacterial and antioxidant bioactivities","volume":"227","author":"Han","year":"2023","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"1720","DOI":"10.1016\/j.ijbiomac.2022.04.129","article-title":"Kappa-carrageenan\/chitosan\/gelatin scaffolds enriched with potassium chloride for bone tissue engineering","volume":"209","author":"Loukelis","year":"2022","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"139112","DOI":"10.1016\/j.ijbiomac.2024.139112","article-title":"A natural gelatin\/casein hydrogel with on-demand adhesion via chitosan solution for wound healing","volume":"290","author":"Zhang","year":"2025","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"20761","DOI":"10.1021\/acsami.3c02108","article-title":"Engineered Injectable Cell-Laden Chitin\/Chitosan Hydrogel with Adhesion and Biodegradability for Calvarial Defect Regeneration","volume":"15","author":"Jiang","year":"2023","journal-title":"ACS Appl. Mater. Interfaces"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"8","DOI":"10.1002\/jbm.b.34359","article-title":"Sustained release of levofloxacin from thermosensitive chitosan-based hydrogel for the treatment of postoperative endophthalmitis","volume":"108","author":"Cheng","year":"2020","journal-title":"J. Biomed. Mater. Res. B Appl. Biomater."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"128525","DOI":"10.1016\/j.ijbiomac.2023.128525","article-title":"Synthesis of aminolyzed gelatin-mediated chitosan as pH-responsive drug-carrying porous scaffolds","volume":"256","author":"Sarwar","year":"2024","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"112773","DOI":"10.1016\/j.colsurfb.2022.112773","article-title":"One-pot self-assembly fabrication of chitosan coated hollow sphere for pH\/ glutathione dual responsive drug delivery","volume":"218","author":"Li","year":"2022","journal-title":"Colloids Surf. B Biointerfaces"},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1016\/j.ijbiomac.2022.12.049","article-title":"The incorporated hydrogel of chitosan-oligoconjugated linoleic acid vesicles and the protective sustained release for curcumin in the gel","volume":"227","author":"Liu","year":"2023","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"258","DOI":"10.1016\/j.actbio.2017.01.060","article-title":"Sustained release of adipose-derived stem cells by thermosensitive chitosan\/gelatin hydrogel for therapeutic angiogenesis","volume":"51","author":"Cheng","year":"2017","journal-title":"Acta Biomater."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"e09306","DOI":"10.1002\/smll.202509306","article-title":"Betulinic Acid Self-Assembled Nanodelivery System Attenuates Osteoarthritis by Dually Modulating Macrophage Polarization and Macrophage-Chondrocyte Crosstalk via Disruption of the GSK3\u03b2\/NF-\u03baB\/CCL20 Axis","volume":"21","author":"Liu","year":"2025","journal-title":"Small"},{"key":"ref_53","doi-asserted-by":"crossref","unstructured":"Lv, T., Chen, Y., Li, N., Liao, X., Heng, Y., Guo, Y., and Hu, K. (2025). A Comprehensive Review of Thermosensitive Hydrogels: Mechanism, Optimization Strategies, and Applications. Gels, 11.","DOI":"10.3390\/gels11070544"},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"123902","DOI":"10.1016\/j.ijbiomac.2023.123902","article-title":"Smart stimuli-responsive chitosan hydrogel for drug delivery: A review","volume":"235","author":"Tian","year":"2023","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"367","DOI":"10.1016\/j.ijbiomac.2022.04.035","article-title":"Thermo-rheological properties of chitosan hydrogels with hydroxypropyl methylcellulose and methylcellulose","volume":"209","author":"Rabelo","year":"2022","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"120954","DOI":"10.1016\/j.ijpharm.2021.120954","article-title":"Thermo-responsive hydrogels for cancer local therapy: Challenges and state-of-art","volume":"606","author":"Rafael","year":"2021","journal-title":"Int. J. Pharm."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"760","DOI":"10.1021\/acsbiomaterials.2c01224","article-title":"Thermosensitive Biodegradable Hydrogels for Local and Controlled Cerebral Delivery of Proteins: MRI-Based Monitoring of In Vitro and In Vivo Protein Release","volume":"9","author":"Yanev","year":"2023","journal-title":"ACS Biomater. Sci. Eng."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"151","DOI":"10.1016\/j.actbio.2022.01.043","article-title":"Thermo-sensitivity and erosion of chitosan crosslinked poly[N-isopropylacrylamide-co-(acrylic acid)-co-(methyl methacrylate)] hydrogels for application to the inferior fornix","volume":"141","author":"Ross","year":"2022","journal-title":"Acta Biomater."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"123454","DOI":"10.1016\/j.carbpol.2025.123454","article-title":"Advanced therapeutic strategies using Thermo-sensitive chitosan\/pectin hydrogel in the treatment of multiple cancers","volume":"357","author":"Pandya","year":"2025","journal-title":"Carbohydr. Polym."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"2393","DOI":"10.1039\/D2MH00314G","article-title":"A versatile strategy to construct free-standing multi-furcated vessels and a complicated vascular network in heterogeneous porous scaffolds via combination of 3D printing and stimuli-responsive hydrogels","volume":"9","author":"Su","year":"2022","journal-title":"Mater. Horiz."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"035007","DOI":"10.1088\/1758-5090\/ab7e74","article-title":"Hierarchical patterning via dynamic sacrificial printing of stimuli-responsive hydrogels","volume":"12","author":"Wen","year":"2020","journal-title":"Biofabrication"},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"e2000454","DOI":"10.1002\/adhm.202000454","article-title":"Decoupled pH- and Thermo-Responsive Injectable Chitosan\/PNIPAM Hydrogel via Thiol-Ene Click Chemistry for Potential Applications in Tissue Engineering","volume":"9","author":"Ding","year":"2020","journal-title":"Adv. Healthc. Mater."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"e2300399","DOI":"10.1002\/mabi.202300399","article-title":"Advances in Stimuli-Responsive Chitosan Hydrogels for Drug Delivery Systems","volume":"24","author":"Xia","year":"2024","journal-title":"Macromol. Biosci."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"1146","DOI":"10.2174\/1567201815666180409102459","article-title":"In vitro Evaluation of Genipin-crosslinked Na-alginate\/Chitosan Hydrogel Films for Delivery of Metformin: Effect of Chitosan Molecular Weight","volume":"15","author":"Ubaid","year":"2018","journal-title":"Curr. Drug Deliv."},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"33584","DOI":"10.1021\/acsami.1c09889","article-title":"Inflammation-Responsive Drug-Loaded Hydrogels with Sequential Hemostasis, Antibacterial, and Anti-Inflammatory Behavior for Chronically Infected Diabetic Wound Treatment","volume":"13","author":"Wang","year":"2021","journal-title":"ACS Appl. Mater. Interfaces"},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"129161","DOI":"10.1016\/j.ijbiomac.2023.129161","article-title":"Pyrogallol loaded chitosan-based polymeric hydrogel for controlling Acinetobacter baumannii wound infections: Synthesis, characterization, and topical application","volume":"259","author":"Abirami","year":"2024","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"119853","DOI":"10.1016\/j.carbpol.2022.119853","article-title":"NIR\/pH-responsive chitosan hydrogels containing Ti3C2\/AuNRs with NIR-triggered photothermal effect","volume":"295","author":"Liu","year":"2022","journal-title":"Carbohydr. Polym."},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"13056","DOI":"10.1021\/acsami.7b00957","article-title":"A G-Quadruplex Hydrogel via Multicomponent Self-Assembly: Formation and Zero-Order Controlled Release","volume":"9","author":"Li","year":"2017","journal-title":"ACS Appl. Mater. Interfaces"},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"e3418","DOI":"10.1002\/btpr.3418","article-title":"Chitosan based hybrid superabsorbent for controlled drug delivery application","volume":"40","author":"Medha","year":"2024","journal-title":"Biotechnol. Prog."},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"771","DOI":"10.1016\/j.bbi.2024.10.028","article-title":"Chemogenetic targeting TRPV1 in obesity-induced depression: Unveiling therapeutic potential of eicosapentaenoic acid and acupuncture","volume":"123","author":"Lin","year":"2025","journal-title":"Brain Behav. Immun."},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"209","DOI":"10.2147\/PTT.S515285","article-title":"Acupoint Injection Combined with BCG-PSN and Thymosin Enteric-Coated Tablets Improve China Han Psoriasis Vulgaris by Regulating T Cell Subsets","volume":"15","author":"Li","year":"2025","journal-title":"Psoriasis"},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"e2200079","DOI":"10.1002\/advs.202200079","article-title":"Transporting Hydrogel via Chinese Acupuncture Needles for Lesion Positioning Therapy","volume":"9","author":"Lin","year":"2022","journal-title":"Adv. Sci."},{"key":"ref_73","doi-asserted-by":"crossref","unstructured":"Li, S., Zhou, H., Zhou, M., Lou, L., Luo, L., and Ji, S. (2025). The effects of acupoint catgut embedding therapy on anthropometric parameters and endocrine function in obese women: A systematic review and meta-analysis. Front. Nutr., 12.","DOI":"10.3389\/fnut.2025.1583556"},{"key":"ref_74","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.msec.2018.02.022","article-title":"In situ formation of injectable chitosan-gelatin hydrogels through double crosslinking for sustained intraocular drug delivery","volume":"88","author":"Song","year":"2018","journal-title":"Mater. Sci. Eng. C Mater. Biol. Appl."},{"key":"ref_75","doi-asserted-by":"crossref","first-page":"365","DOI":"10.1016\/j.ijbiomac.2021.03.017","article-title":"Development of a dual delivery of levofloxacin and prednisolone acetate via PLGA nanoparticles\/ thermosensitive chitosan-based hydrogel for postoperative management: An in-vitro and ex-vivo study","volume":"180","author":"Cheng","year":"2021","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_76","doi-asserted-by":"crossref","first-page":"110419","DOI":"10.1016\/j.msec.2019.110419","article-title":"In vitro and in vivo evaluation of 3D biodegradable thermo\/pH sensitive sol-gel reversible hydroxybutyl chitosan hydrogel","volume":"108","author":"Li","year":"2020","journal-title":"Mater. Sci. Eng. C Mater. Biol. Appl."},{"key":"ref_77","doi-asserted-by":"crossref","first-page":"124776","DOI":"10.1016\/j.ijpharm.2024.124776","article-title":"Optimization of chitosan-gelatin-based 3D-printed scaffolds for tissue engineering and drug delivery applications","volume":"666","author":"Palamidi","year":"2024","journal-title":"Int. J. Pharm."},{"key":"ref_78","doi-asserted-by":"crossref","first-page":"3561","DOI":"10.1039\/D2BM01795D","article-title":"T cell-loaded injectable chitosan scaffold shows short-term efficacy in localised cancer immunotherapy in mice","volume":"11","author":"Cunningham","year":"2023","journal-title":"Biomater. Sci."},{"key":"ref_79","doi-asserted-by":"crossref","first-page":"118522","DOI":"10.1016\/j.carbpol.2021.118522","article-title":"Thermomechanically processed chitosan:gelatin films being transparent, mechanically robust and less hygroscopic","volume":"272","author":"Chen","year":"2021","journal-title":"Carbohydr. Polym."},{"key":"ref_80","doi-asserted-by":"crossref","first-page":"148591","DOI":"10.1016\/j.ijbiomac.2025.148591","article-title":"Diprophylline drug as an efficient inducer loaded in chitosan-gelatin-umbilical cord serum hydrogel for neural differentiation of human Wharton\u2019s jelly-derived mesenchymal stem cells in vitro","volume":"335","author":"Shemshadi","year":"2026","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_81","doi-asserted-by":"crossref","first-page":"278","DOI":"10.1016\/j.ijpharm.2017.12.045","article-title":"Accelerated full-thickness wound healing via sustained bFGF delivery based on a PVA\/chitosan\/gelatin hydrogel incorporating PCL microspheres","volume":"537","author":"Shamloo","year":"2018","journal-title":"Int. J. Pharm."},{"key":"ref_82","doi-asserted-by":"crossref","first-page":"179","DOI":"10.1016\/j.exer.2018.11.017","article-title":"Thermosensitive chitosan-gelatin-based hydrogel containing curcumin-loaded nanoparticles and latanoprost as a dual-drug delivery system for glaucoma treatment","volume":"179","author":"Cheng","year":"2019","journal-title":"Exp. Eye Res."},{"key":"ref_83","doi-asserted-by":"crossref","first-page":"2111","DOI":"10.1002\/jbm.a.36970","article-title":"Sustained release of human platelet lysate growth factors by thermosensitive hydroxybutyl chitosan hydrogel promotes skin wound healing in rats","volume":"108","author":"Lim","year":"2020","journal-title":"J. Biomed. Mater. Res. A"},{"key":"ref_84","doi-asserted-by":"crossref","first-page":"558","DOI":"10.1080\/03639045.2016.1270960","article-title":"Design and optimization of thermosensitive nanoemulsion hydrogel for sustained-release of praziquantel","volume":"43","author":"Cong","year":"2017","journal-title":"Drug Dev. Ind. Pharm."},{"key":"ref_85","doi-asserted-by":"crossref","first-page":"e08697","DOI":"10.1016\/j.heliyon.2021.e08697","article-title":"Development of topical chitosan\/ \u03b2-glycerophosphate-based hydrogel loaded with levofloxacin in the treatment of keratitis: An ex-vivo study","volume":"8","author":"Chang","year":"2022","journal-title":"Heliyon"},{"key":"ref_86","doi-asserted-by":"crossref","first-page":"432","DOI":"10.1186\/s12951-024-02722-y","article-title":"The combination of modified acupuncture needle and melittin hydrogel as a novel therapeutic approach for rheumatoid arthritis treatment","volume":"22","author":"Liu","year":"2024","journal-title":"J. Nanobiotechnol."},{"key":"ref_87","doi-asserted-by":"crossref","first-page":"9636897221126088","DOI":"10.1177\/09636897221126088","article-title":"Dexamethasone-Loaded Injectable In-situ Thermal Crosslinking Magnetic Responsive Hydrogel for the Physiochemical Stimulation of Acupoint to Suppress Pain in Sciatica Rats","volume":"31","author":"Wei","year":"2022","journal-title":"Cell Transplant."},{"key":"ref_88","doi-asserted-by":"crossref","first-page":"123268","DOI":"10.1016\/j.biomaterials.2025.123268","article-title":"Biodegradable, electroconductive self-healing hydrogel based on polydopamine-coated polyurethane nano-crosslinker for Parkinson\u2019s disease therapy","volume":"320","author":"Chen","year":"2025","journal-title":"Biomaterials"},{"key":"ref_89","doi-asserted-by":"crossref","first-page":"120608","DOI":"10.1016\/j.biomaterials.2020.120608","article-title":"Cryogel\/hydrogel biomaterials and acupuncture combined to promote diabetic skin wound healing through immunomodulation","volume":"269","author":"Chen","year":"2021","journal-title":"Biomaterials"},{"key":"ref_90","doi-asserted-by":"crossref","first-page":"102335","DOI":"10.1016\/j.mtbio.2025.102335","article-title":"Electroacupuncture at Huatuo-Jiaji acupoints combined with conductive hydrogel synergistically improves bladder and motor functions after spinal cord injury","volume":"35","author":"Zhang","year":"2025","journal-title":"Mater. Today Bio"},{"key":"ref_91","first-page":"929","article-title":"Location, selection, and comparative anatomy of \u201cTaixi\u201d (KI3), \u201cShuiquan\u201d (KI5), \u201cFuliu\u201d (KI7), \u201cJiaoxin\u201d (KI8), \u201cZhubin\u201d (KI9), and \u201cYingu\u201d (KI10) acupoints in rabbits","volume":"45","author":"Ren","year":"2020","journal-title":"Zhen Ci Yan Jiu"},{"key":"ref_92","doi-asserted-by":"crossref","first-page":"203","DOI":"10.5455\/OVJ.2021.v11.i2.3","article-title":"The scientific basis of acupuncture for veterinary pain management: A review based on relevant literature from the last two decades","volume":"11","author":"Dewey","year":"2021","journal-title":"Open Vet. J."},{"key":"ref_93","doi-asserted-by":"crossref","first-page":"8669","DOI":"10.1039\/D0CS00461H","article-title":"Inorganic nanomaterials with rapid clearance for biomedical applications","volume":"50","author":"Wang","year":"2021","journal-title":"Chem. Soc. Rev."},{"key":"ref_94","doi-asserted-by":"crossref","first-page":"360","DOI":"10.1016\/j.jams.2017.08.002","article-title":"Comparison of Point Placement by Veterinary Professionals with Different Levels of Acupuncture Training in a Canine Cadaver Model","volume":"10","author":"Yang","year":"2017","journal-title":"J. Acupunct. Meridian Stud."},{"key":"ref_95","doi-asserted-by":"crossref","first-page":"146011","DOI":"10.1016\/j.ijbiomac.2025.146011","article-title":"Chitosan-based nanomaterials: Pioneering a review in veterinary medicine applications","volume":"320","author":"Yang","year":"2025","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_96","doi-asserted-by":"crossref","unstructured":"Milano, F., Masi, A., Madaghiele, M., Sannino, A., Salvatore, L., and Gallo, N. (2023). Current Trends in Gelatin-Based Drug Delivery Systems. Pharmaceutics, 15.","DOI":"10.3390\/pharmaceutics15051499"},{"key":"ref_97","doi-asserted-by":"crossref","first-page":"1430","DOI":"10.3390\/foods11101430","article-title":"Antibiotic Use in Livestock and Residues in Food-A Public Health Threat: A Review","volume":"11","author":"Ghimpeteanu","year":"2022","journal-title":"Foods"},{"key":"ref_98","doi-asserted-by":"crossref","first-page":"177","DOI":"10.1017\/S1466252317000068","article-title":"A scoping review of the evidence for efficacy of acupuncture in companion animals","volume":"18","author":"Rose","year":"2017","journal-title":"Anim. Health Res. Rev."},{"key":"ref_99","doi-asserted-by":"crossref","first-page":"110633","DOI":"10.1016\/j.vetmic.2025.110633","article-title":"A recombinant adenovirus-vectored PEDV vaccine co-expressing S1 and N proteins enhances mucosal immunity and confers protection in piglets","volume":"308","author":"Luo","year":"2025","journal-title":"Vet. Microbiol."},{"key":"ref_100","doi-asserted-by":"crossref","first-page":"e0077724","DOI":"10.1128\/msphere.00777-24","article-title":"Astragalus polysaccharide enhances maternal mucosal immunity against PEDV","volume":"9","author":"Tang","year":"2024","journal-title":"mSphere"},{"key":"ref_101","doi-asserted-by":"crossref","unstructured":"Qin, W., Yu, Z., Li, Z., Liu, H., Li, W., Zhao, J., Ren, Y., and Ma, L. (2023). Dietary Berberine and Ellagic Acid Supplementation Improve Growth Performance and Intestinal Damage by Regulating the Structural Function of Gut Microbiota and SCFAs in Weaned Piglets. Microorganisms, 11.","DOI":"10.3390\/microorganisms11051254"},{"key":"ref_102","doi-asserted-by":"crossref","first-page":"853","DOI":"10.1111\/jvim.16057","article-title":"Prevalence of gastrointestinal lesions in dogs chronically treated with nonsteroidal anti-inflammatory drugs","volume":"35","author":"Mabry","year":"2021","journal-title":"J. Vet. Intern. Med."},{"key":"ref_103","first-page":"941","article-title":"Effect of acupuncture on pain and quality of life in canine neurological and musculoskeletal diseases","volume":"58","author":"Silva","year":"2017","journal-title":"Can. Vet. J."},{"key":"ref_104","doi-asserted-by":"crossref","first-page":"1031","DOI":"10.2460\/javma.249.9.1031","article-title":"Owner assessment of chronic pain intensity and results of gait analysis of dogs with hip dysplasia treated with acupuncture","volume":"249","author":"Teixeira","year":"2016","journal-title":"J. Am. Vet. Med. Assoc."},{"key":"ref_105","doi-asserted-by":"crossref","first-page":"2236","DOI":"10.1016\/j.csbj.2021.04.010","article-title":"Spatial topological analysis of sympathetic neurovascular characteristic of acupoints in Ren meridian using advanced tissue-clearing and near infrared II imaging","volume":"19","author":"Hu","year":"2021","journal-title":"Comput. Struct. Biotechnol. J."},{"key":"ref_106","doi-asserted-by":"crossref","first-page":"189","DOI":"10.1002\/JLB.3AB0420-361RR","article-title":"The neuro-immune microenvironment of acupoints-initiation of acupuncture effectiveness","volume":"108","author":"Gong","year":"2020","journal-title":"J. Leukoc. Biol."},{"key":"ref_107","doi-asserted-by":"crossref","first-page":"60","DOI":"10.14202\/vetworld.2025.60-66","article-title":"Effectiveness of acupuncture for equine laminitis: Systematic review and meta-analysis","volume":"18","author":"Fikri","year":"2025","journal-title":"Vet. World"},{"key":"ref_108","doi-asserted-by":"crossref","first-page":"2681","DOI":"10.3390\/ani14182681","article-title":"Revealing the Therapeutic Potential of Muscle-Derived Mesenchymal Stem\/Stromal Cells: An In Vitro Model for Equine Laminitis Based on Activated Neutrophils, Anoxia-Reoxygenation, and Myeloperoxidase","volume":"14","author":"Serteyn","year":"2024","journal-title":"Animals"},{"key":"ref_109","doi-asserted-by":"crossref","first-page":"549","DOI":"10.1016\/j.cveq.2021.08.004","article-title":"Pharmacology of the Equine Foot: Medical Pain Management for Laminitis","volume":"37","author":"Hopster","year":"2021","journal-title":"Vet. Clin. N. Am. Equine Pract."},{"key":"ref_110","doi-asserted-by":"crossref","first-page":"4789","DOI":"10.5455\/OVJ.2025.v15.i10.1","article-title":"Efficacy of acupuncture in racehorses: A review","volume":"15","author":"Purnama","year":"2025","journal-title":"Open Vet. J."},{"key":"ref_111","doi-asserted-by":"crossref","unstructured":"Calatayud-Bonilla, M., Carmona, J.U., and Prades, M. (2025). Clinical Effectiveness of Dry Needling on Myofascial Trigger Points in Horses: A Prospective Algometric Controlled Study. Animals, 15.","DOI":"10.3390\/ani15152207"},{"key":"ref_112","doi-asserted-by":"crossref","unstructured":"Valentino, A., Gutierrez-Quintana, R., and Olby, N. (2025). Breed-specific variations in canine spinal cord anatomy: Conus medullaris and dural sac termination and filum terminale internum length. BMC Vet. Res., 21.","DOI":"10.1186\/s12917-025-05068-1"},{"key":"ref_113","doi-asserted-by":"crossref","first-page":"122671","DOI":"10.1016\/j.ijpharm.2023.122671","article-title":"A review of conventional and emerging technologies for hydrogels sterilization","volume":"634","author":"Bento","year":"2023","journal-title":"Int. J. Pharm."},{"key":"ref_114","doi-asserted-by":"crossref","first-page":"6270","DOI":"10.1039\/D5BM01305D","article-title":"Effects of irradiation sterilization on the physicochemical and functional properties of commercial biomedical hydrogels","volume":"13","author":"Zhou","year":"2025","journal-title":"Biomater. Sci."},{"key":"ref_115","doi-asserted-by":"crossref","first-page":"4359","DOI":"10.1021\/acsbiomaterials.4c00723","article-title":"Interaction-Based Perspective for Designing Polymer Biomaterial: A Strategic Approach to the Chitosan-Glycerophosphate System","volume":"10","author":"Das","year":"2024","journal-title":"ACS Biomater. Sci. 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