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This study used ethanol precipitation and trichloroacetic acid deproteinization to extract crude exopolysaccharide from the fermentation broth of Bacillus amyloliquefaciens SQ-2. The pure fraction, EPS-3791, was obtained using Sephadex G-100 gel filtration chromatography and DEAE cellulose ion exchange. The weight\u2013average molecular weight of EPS-3791 was 64.4 kDa. Monosaccharide analysis indicated fructan as the dominant component, which was consistent with the results of methylation analysis and NMR spectroscopy, confirming that EPS-3791 is a fructan mainly linked by \u21921)\u2013Fruf\u2013(2\u2192bonds. UV scanning indicated high purity. FTIR analysis revealed functional groups including hydroxyl, carbonyl, and C\u2013O\u2013C groups. EPS-3791 exhibited a porous three-dimensional network morphology by SEM, with a decomposition temperature of 191.61 \u00b0C by TGA. Additionally, aerogels were prepared by freeze drying. EPS-3791 aerogels demonstrated minimal cytotoxicity to normal L929 cells while inhibiting the growth of human lung cancer A549, breast cancer MCF\u20137, and cervical cancer HeLa cells in a dose-dependent manner. Scratch wound healing experiments revealed that EPS-3791 aerogels hindered HeLa cell migration while promoting L929 wound closure. These findings identify EPS-3791 as a fructan-type exopolysaccharide aerogel with specific anticancer properties.<\/jats:p>","DOI":"10.3390\/gels12060462","type":"journal-article","created":{"date-parts":[[2026,5,25]],"date-time":"2026-05-25T14:10:22Z","timestamp":1779718222000},"page":"462","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Fabrication and Characterization of Bio-Based Aerogels Derived from Bacillus amyloliquefaciens SQ-2 Exopolysaccharides: Structural Characterization and In Vitro Antitumor Activity Analysis"],"prefix":"10.3390","volume":"12","author":[{"given":"Tianjiao","family":"Zhao","sequence":"first","affiliation":[{"name":"Tianjin Key Laboratory of Organic Solar Cells and Photochemical Conversion, College of Chemistry and Chemical Engineering, Tianjin University of Technology, Tianjin 300384, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3490-3393","authenticated-orcid":false,"given":"Lei","family":"Huang","sequence":"additional","affiliation":[{"name":"Tianjin Key Laboratory of Organic Solar Cells and Photochemical Conversion, College of Chemistry and Chemical Engineering, Tianjin University of Technology, Tianjin 300384, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sihan","family":"Wei","sequence":"additional","affiliation":[{"name":"Tianjin Key Laboratory of Organic Solar Cells and Photochemical Conversion, College of Chemistry and Chemical Engineering, Tianjin University of Technology, Tianjin 300384, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chengci","family":"Liu","sequence":"additional","affiliation":[{"name":"Tianjin Key Laboratory of Organic Solar Cells and Photochemical Conversion, College of Chemistry and Chemical Engineering, Tianjin University of Technology, Tianjin 300384, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinhua","family":"Xu","sequence":"additional","affiliation":[{"name":"Chinese Academy of Fishery Sciences, Beijing 100141, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lu","family":"Qiao","sequence":"additional","affiliation":[{"name":"Chinese Academy of Fishery Sciences, Beijing 100141, China"},{"name":"Hainan Fisheries Innovation Research Institute, Chinese Academy of Fishery Sciences, Sanya 572000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jincheng","family":"Li","sequence":"additional","affiliation":[{"name":"Chinese Academy of Fishery Sciences, Beijing 100141, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chaoying","family":"Zhang","sequence":"additional","affiliation":[{"name":"Chinese Academy of Fishery Sciences, Beijing 100141, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yingchun","family":"Mu","sequence":"additional","affiliation":[{"name":"Chinese Academy of Fishery Sciences, Beijing 100141, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhiyang","family":"Zhao","sequence":"additional","affiliation":[{"name":"Jiangsu Key Laboratory of New Energy Devices & Interface Science, School of Chemistry and Materials Science, Nanjing University of Information Science and Technology, Nanjing 210044, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-3830-4594","authenticated-orcid":false,"given":"Meitong","family":"Li","sequence":"additional","affiliation":[{"name":"Tianjin Key Laboratory of Organic Solar Cells and Photochemical Conversion, College of Chemistry and Chemical Engineering, Tianjin University of Technology, Tianjin 300384, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9615-6920","authenticated-orcid":false,"given":"Xin","family":"Hu","sequence":"additional","affiliation":[{"name":"Chinese Academy of Fishery Sciences, Beijing 100141, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,5,25]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1475","DOI":"10.1080\/10408398.2019.1575791","article-title":"Current status of biotechnological production and applications of microbial exopolysaccharides","volume":"60","author":"Barcelos","year":"2020","journal-title":"Crit. 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