{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,26]],"date-time":"2026-06-26T01:48:25Z","timestamp":1782438505382,"version":"3.54.5"},"reference-count":63,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2026,6,25]],"date-time":"2026-06-25T00:00:00Z","timestamp":1782345600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Postgraduate Research & Practice Innovation Program of Jiangsu Province","award":["KYCX2023-123"],"award-info":[{"award-number":["KYCX2023-123"]}]},{"name":"Innovation Ability Improvement Project of Science and Technology SMEs in Shandong Province","award":["2025TSGCCZZB0913"],"award-info":[{"award-number":["2025TSGCCZZB0913"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Gels"],"abstract":"<jats:p>Folate is an essential vitamin associated with protein and DNA synthesis in the body. Compared with synthetic folic acid, 6S-5-methyltetrahydrofolate calcium salt crystal form C (MTHF CAC) is safer and has a higher bioavailability. In this study, a nanofiber membrane (MT-GE) was prepared from fish gelatin and MTHF CAC in the aqueous system via electrospinning. Differential scanning calorimetry showed higher transition temperatures for MT-GE than for GE. The weight loss curve of MT-GE detected by thermogravimetric analysis was higher than that of GE. The results corresponded to those of X-ray diffraction, which indicated the slightly higher crystalline strength of MT-GE than GE. Therefore, the inclusion of MTHF CAC improved the physical characteristics of GE nanofibers. High-performance liquid chromatography analysis revealed that the retention of MTHF CAC in MT-GE reached 85.57%, which suggested that electrospinning caused no effect on the properties of MTHF CAC. The MT-GE membrane supported cell proliferation, and the Cell Counting Kit-8 results indicated that the cell proliferation rate exceeded 100%, with the MT-GE solution demonstrating more than double the proliferation rate of the control group. Therefore, MT-GE has great potential for use as a medical biomaterial.<\/jats:p>","DOI":"10.3390\/gels12070563","type":"journal-article","created":{"date-parts":[[2026,6,26]],"date-time":"2026-06-26T00:49:23Z","timestamp":1782434963000},"page":"563","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Study on the Preparation and Properties of MT-GE (6S-5-Methyltetrahydrofolate Calcium Salt Crystal Form C-Gelatin) Nanofiber Membrane"],"prefix":"10.3390","volume":"12","author":[{"given":"Yuhang","family":"Wang","sequence":"first","affiliation":[{"name":"School of Ocean Food and Bioengineering, Jiangsu Ocean University, Lianyungang 222005, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ke","family":"Wang","sequence":"additional","affiliation":[{"name":"School of Ocean Food and Bioengineering, Jiangsu Ocean University, Lianyungang 222005, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mochi","family":"Zhu","sequence":"additional","affiliation":[{"name":"School of Ocean Food and Bioengineering, Jiangsu Ocean University, Lianyungang 222005, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu","family":"Liu","sequence":"additional","affiliation":[{"name":"School of Ocean Food and Bioengineering, Jiangsu Ocean University, Lianyungang 222005, China"},{"name":"Jiangsu Institute of Marine Resources Development, Jiangsu Ocean University, Lianyungang 222005, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tianyue","family":"Xu","sequence":"additional","affiliation":[{"name":"School of Ocean Food and Bioengineering, Jiangsu Ocean University, Lianyungang 222005, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rui","family":"Duan","sequence":"additional","affiliation":[{"name":"School of Marine Science and Fisheries, Jiangsu Ocean University, Lianyungang 222005, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junjie","family":"Zhang","sequence":"additional","affiliation":[{"name":"School of Ocean Food and Bioengineering, Jiangsu Ocean University, Lianyungang 222005, China"},{"name":"Jiangsu Institute of Marine Resources Development, Jiangsu Ocean University, Lianyungang 222005, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,6,25]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1016\/j.tifs.2014.03.004","article-title":"Electrospinning and Electrospraying Techniques: Potential Food Based Applications","volume":"38","author":"Bhushani","year":"2014","journal-title":"Trends Food Sci. 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