{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,12]],"date-time":"2026-08-12T09:30:41Z","timestamp":1786527041867,"version":"build-2736575974"},"reference-count":37,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2026,8,12]],"date-time":"2026-08-12T00:00:00Z","timestamp":1786492800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001807","name":"FAPESP","doi-asserted-by":"publisher","award":["2019\/26070-1"],"award-info":[{"award-number":["2019\/26070-1"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]},{"award":["2019\/26070-1"],"award-info":[{"award-number":["2019\/26070-1"]}],"id":[{"id":"https:\/\/ror.org\/02ddkpn78","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Gels"],"abstract":"<jats:p>This study evaluated plasma gel preparation protocols with potential to yield more biocompatible materials for facial aesthetic applications. Human dermal fibroblasts were cultured in medium supplemented with 20% plasma gel obtained using four protocols: G1, centrifugation at 580 g for 8 min in tubes with anticoagulant followed by activation with 10% calcium chloride; G2, centrifugation at 580 g for 8 min in tubes with anticoagulant without activation; G3, centrifugation at 210 g for 8 min in tubes without anticoagulant with activation; and G4, centrifugation at 210 g for 8 min in tubes without anticoagulant and without activation. All protocols involved heating plasma at 76 \u00b0C for 12 min to produce gels. Cell viability was assessed using MTT and Crystal Violet assays over 72 h. Cell morphology and fibrin formation were analyzed by hematoxylin\/eosin staining. Cell migration was evaluated using a wound healing assay. RT-qPCR analysis of COL1A1 and FN1 was performed after 24, 48, and 72 h. Fibroblasts cultured with plasma gel from G3 and G4 showed significantly higher viability compared with the control group. G1 and G2 exhibited increased fibrin formation and reduced cell density. Slower wound closure was observed in G2, which was consistent with increased late-stage expression of COL1A1 and FN1. In the conditions evaluated, low-speed centrifugation and the use of tubes without anticoagulant improved plasma gel biocompatibility. Lack of activation after high-speed centrifugation was associated with slower wound closure.<\/jats:p>","DOI":"10.3390\/gels12080713","type":"journal-article","created":{"date-parts":[[2026,8,12]],"date-time":"2026-08-12T07:57:26Z","timestamp":1786521446000},"page":"713","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Effect of Plasma Gel on Viability, Proliferation and Migration of Dermal Human Fibroblasts In Vitro"],"prefix":"10.3390","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0009-0004-3441-3230","authenticated-orcid":false,"given":"Camila","family":"Slompo","sequence":"first","affiliation":[{"name":"Department of Biological Sciences, Bauru School of Dentistry, University of S\u00e3o Paulo, Al. Dr. Oct\u00e1vio Pinheiro Brisolla, 9-75, Bauru 17012-901, S\u00e3o Paulo, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emanuelle Pangoni de","family":"Carvalho","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, Bauru School of Dentistry, University of S\u00e3o Paulo, Al. Dr. Oct\u00e1vio Pinheiro Brisolla, 9-75, Bauru 17012-901, S\u00e3o Paulo, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-2815-4878","authenticated-orcid":false,"given":"Thayna Kathllen da","family":"Silva","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, Bauru School of Dentistry, University of S\u00e3o Paulo, Al. Dr. Oct\u00e1vio Pinheiro Brisolla, 9-75, Bauru 17012-901, S\u00e3o Paulo, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-6236-8817","authenticated-orcid":false,"given":"Byron Israel Proa\u00f1o","family":"Roldan","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, Bauru School of Dentistry, University of S\u00e3o Paulo, Al. Dr. Oct\u00e1vio Pinheiro Brisolla, 9-75, Bauru 17012-901, S\u00e3o Paulo, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7452-661X","authenticated-orcid":false,"given":"Juliana Sanches","family":"Trevizol","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, Bauru School of Dentistry, University of S\u00e3o Paulo, Al. Dr. Oct\u00e1vio Pinheiro Brisolla, 9-75, Bauru 17012-901, S\u00e3o Paulo, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elizandra Paccola Moretto de","family":"Almeida","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, Bauru School of Dentistry, University of S\u00e3o Paulo, Al. Dr. Oct\u00e1vio Pinheiro Brisolla, 9-75, Bauru 17012-901, S\u00e3o Paulo, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adriano de Souza","family":"Pessoa","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, Bauru School of Dentistry, University of S\u00e3o Paulo, Al. Dr. Oct\u00e1vio Pinheiro Brisolla, 9-75, Bauru 17012-901, S\u00e3o Paulo, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4507-5148","authenticated-orcid":false,"given":"Thais Francini","family":"Garbieri","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, Bauru School of Dentistry, University of S\u00e3o Paulo, Al. Dr. Oct\u00e1vio Pinheiro Brisolla, 9-75, Bauru 17012-901, S\u00e3o Paulo, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mariana Liessa Rovis","family":"Sanches","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, Bauru School of Dentistry, University of S\u00e3o Paulo, Al. Dr. Oct\u00e1vio Pinheiro Brisolla, 9-75, Bauru 17012-901, S\u00e3o Paulo, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3070-5960","authenticated-orcid":false,"given":"Rodrigo Cardoso de","family":"Oliveira","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, Bauru School of Dentistry, University of S\u00e3o Paulo, Al. Dr. Oct\u00e1vio Pinheiro Brisolla, 9-75, Bauru 17012-901, S\u00e3o Paulo, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5985-3951","authenticated-orcid":false,"given":"Mar\u00edlia Afonso Rabelo","family":"Buzalaf","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, Bauru School of Dentistry, University of S\u00e3o Paulo, Al. Dr. Oct\u00e1vio Pinheiro Brisolla, 9-75, Bauru 17012-901, S\u00e3o Paulo, Brazil"},{"name":"Hospital for Rehabilitation of Craniofacial Anomalies, University of S\u00e3o Paulo (HRAC\/USP), Bauru 17012-902, S\u00e3o Paulo, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,8,12]]},"reference":[{"key":"ref_1","first-page":"179","article-title":"Platelet Rich Plasma (PRP) for Facial Rejuvenation","volume":"XXXVII","author":"Zenker","year":"2010","journal-title":"J. M\u00e9d. Esth."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"e20220020","DOI":"10.1590\/1678-7757-2022-0020","article-title":"Autologous Platelet Concentrates for Facial Rejuvenation","volume":"30","author":"Buzalaf","year":"2022","journal-title":"J. Appl. 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Surg."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"634","DOI":"10.12669\/pjms.39.3.7264","article-title":"Comparison of Single and Double Centrifugation Methods for Preparation of Platelet-Rich Plasma (PRP)","volume":"39","author":"Saqlain","year":"2023","journal-title":"Pak. J. Med. Sci."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"1421","DOI":"10.1111\/jocd.13742","article-title":"Injectable Platelet-Rich Fibrin (PRF): The Newest Biomaterial and Its Use in Various Dermatological Conditions in Our Practice: A Case Series","volume":"20","author":"Shashank","year":"2021","journal-title":"J. Cosmet. 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