{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,29]],"date-time":"2026-10-29T17:48:01Z","timestamp":1793296081816,"version":"4.5.0"},"reference-count":68,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T00:00:00Z","timestamp":1787097600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Scientific Research Project of Fuyang Municipal Health Commission","award":["FY2023-004"],"award-info":[{"award-number":["FY2023-004"]}]},{"name":"Scientific Research Project of Fuyang Municipal Health Commission","award":["FYZC2024-015"],"award-info":[{"award-number":["FYZC2024-015"]}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32371416"],"award-info":[{"award-number":["32371416"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"award":["32371416"],"award-info":[{"award-number":["32371416"]}],"id":[{"id":"https:\/\/ror.org\/01h0zpd94","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Gels"],"abstract":"<jats:p>Cell-laden hydrogel bioinks for extrusion bioprinting are commonly evaluated by precursor rheology, extrusion behavior, and immediate shape fidelity, yet these measures do not establish whether printed constructs remain stable and functional during culture or tissue maturation. This review examines the post-printing evolution of cell-laden extrusion-printed hydrogel constructs, prioritizing direct evidence from cell-laden prints and using acellular prints and bulk hydrogels only to clarify mechanisms. Maturation is organized into immediate stabilization, network evolution and environmental equilibration, and long-term remodeling. Crosslinking, hydration, ion exchange, stress relaxation, degradation, cellular contraction, and matrix deposition may support maturation or cause structural, mechanical, interfacial, transport, and biofunctional failure. We propose a conceptual, research-oriented use-point assessment framework that compares each construct with relevant reference states and application-specific requirements after stabilization, during culture, and before intended use. The framework links the earliest observed critical deviation to relevant measurements, targeted redesign, and reassessment under the same conditions. A cartilage construct illustrates sequential evaluation of geometry, wet-state mechanics, cell distribution, and matrix formation. Current evidence is limited by inconsistent assessment times, incomplete reporting, and few integrated longitudinal studies. Future work should standardize maturation histories, model construct trajectories, and prospectively validate product-specific criteria. Evaluation should focus on the complete cell-laden extrusion-printed construct at its intended use point rather than on the freshly printed filament.<\/jats:p>","DOI":"10.3390\/gels12080742","type":"journal-article","created":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T13:14:01Z","timestamp":1787145241000},"page":"742","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["After the Nozzle: Post-Printing Maturation, Failure Modes, and Use-Point Assessment of Cell-Laden Extrusion-Bioprinted Hydrogel Constructs"],"prefix":"10.3390","volume":"12","author":[{"given":"Yifan","family":"Li","sequence":"first","affiliation":[{"name":"Key Laboratory of Neuroregeneration of Jiangsu and Ministry of Education, Co-Innovation Center of Neuroregeneration, NMPA Key Laboratory for Research and Evaluation of Tissue Engineering Technology Products, Nantong University, Nantong 226001, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiuyu","family":"Wen","sequence":"additional","affiliation":[{"name":"Key Laboratory of Neuroregeneration of Jiangsu and Ministry of Education, Co-Innovation Center of Neuroregeneration, NMPA Key Laboratory for Research and Evaluation of Tissue Engineering Technology Products, Nantong University, Nantong 226001, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Linken","family":"Li","sequence":"additional","affiliation":[{"name":"Key Laboratory of Neuroregeneration of Jiangsu and Ministry of Education, Co-Innovation Center of Neuroregeneration, NMPA Key Laboratory for Research and Evaluation of Tissue Engineering Technology Products, Nantong University, Nantong 226001, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li","family":"Li","sequence":"additional","affiliation":[{"name":"Key Laboratory of Neuroregeneration of Jiangsu and Ministry of Education, Co-Innovation Center of Neuroregeneration, NMPA Key Laboratory for Research and Evaluation of Tissue Engineering Technology Products, Nantong University, Nantong 226001, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianghong","family":"He","sequence":"additional","affiliation":[{"name":"Key Laboratory of Neuroregeneration of Jiangsu and Ministry of Education, Co-Innovation Center of Neuroregeneration, NMPA Key Laboratory for Research and Evaluation of Tissue Engineering Technology Products, Nantong University, Nantong 226001, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,8,19]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"034106","DOI":"10.1088\/1758-5090\/aacdc7","article-title":"Optimization of gelatin-alginate composite bioink printability using rheological parameters: A systematic approach","volume":"10","author":"Gao","year":"2018","journal-title":"Biofabrication"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"014102","DOI":"10.1088\/1758-5090\/aa90e2","article-title":"Assessing bioink shape fidelity to aid material development in 3D bioprinting","volume":"10","author":"Ribeiro","year":"2017","journal-title":"Biofabrication"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"045006","DOI":"10.1088\/1758-5090\/ab97a1","article-title":"Direct-write 3D printing and characterization of a GelMA-based biomaterial for intracorporeal tissue engineering","volume":"12","author":"Adib","year":"2020","journal-title":"Biofabrication"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"100799","DOI":"10.1016\/j.mtbio.2023.100799","article-title":"Advantages of photo-curable collagen-based cell-laden bioinks compared to methacrylated gelatin (GelMA) in digital light processing (DLP) and extrusion bioprinting","volume":"23","author":"Shi","year":"2023","journal-title":"Mater. Today Bio"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"326","DOI":"10.1038\/nmat4489","article-title":"Hydrogels with tunable stress relaxation regulate stem cell fate and activity","volume":"15","author":"Chaudhuri","year":"2016","journal-title":"Nat. Mater."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"104251","DOI":"10.1016\/j.isci.2022.104251","article-title":"Non-destructive mechanical assessment for optimization of 3D bioprinted soft tissue scaffolds","volume":"25","author":"Godau","year":"2022","journal-title":"iScience"},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Khatun, M.R., Bhattacharyya, A., Gunbayar, M., Jung, M., and Noh, I. (2023). Study on Bioresponsive Gelatin-Hyaluronic Acid-Genipin Hydrogel for High Cell-Density 3D Bioprinting. Gels, 9.","DOI":"10.20944\/preprints202306.2020.v1"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"e2100206","DOI":"10.1002\/adhm.202100206","article-title":"Tuning Superfast Curing Thiol-Norbornene-Functionalized Gelatin Hydrogels for 3D Bioprinting","volume":"10","author":"Gockler","year":"2021","journal-title":"Adv. Healthc. Mater."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"e00376","DOI":"10.1002\/marc.202500376","article-title":"Effect of Photoinitiation Process on Photo-Crosslinking of Gelatin Methacryloyl Hydrogel Networks","volume":"46","author":"Duymaz","year":"2025","journal-title":"Macromol. Rapid Commun."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"12076","DOI":"10.1021\/acsomega.2c00335","article-title":"Three-Dimensional Printing Self-Healing Dynamic\/Photocrosslinking Gelatin-Hyaluronic Acid Double-Network Hydrogel for Tissue Engineering","volume":"7","author":"Wang","year":"2022","journal-title":"ACS Omega"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"112237","DOI":"10.1016\/j.matdes.2023.112237","article-title":"The correlation between rheological properties and extrusion-based printability in bioink artifact quantification","volume":"233","author":"Gillispie","year":"2023","journal-title":"Mater. Des."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"13972","DOI":"10.1038\/s41598-024-64039-y","article-title":"Advanced optical assessment and modeling of extrusion bioprinting","volume":"14","author":"Lamberger","year":"2024","journal-title":"Sci. Rep."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"025004","DOI":"10.1088\/1758-5090\/adaa22","article-title":"In-situ quality monitoring during embedded bioprinting using integrated microscopy and classical computer vision","volume":"17","author":"Sergis","year":"2025","journal-title":"Biofabrication"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"1211","DOI":"10.1038\/s41598-023-28286-9","article-title":"Predicting and elucidating the post-printing behavior of 3D printed cancer cells in hydrogel structures by integrating in-vitro and in-silico experiments","volume":"13","author":"Mohammadrezaei","year":"2023","journal-title":"Sci. Rep."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"2424553","DOI":"10.1002\/adfm.202424553","article-title":"Autonomous Control of Extrusion Bioprinting Using Convolutional Neural Networks","volume":"35","author":"Kelly","year":"2025","journal-title":"Adv. Funct. Mater."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"609","DOI":"10.1177\/0885328218805864","article-title":"Reversible physical crosslinking strategy with optimal temperature for 3D bioprinting of human chondrocyte-laden gelatin methacryloyl bioink","volume":"33","author":"Gu","year":"2018","journal-title":"J. Biomater. Appl."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"1815","DOI":"10.1021\/acsabm.0c00169","article-title":"3D Bioprinting of Methylcellulose\/Gelatin-Methacryloyl (MC\/GelMA) Bioink with High Shape Integrity","volume":"3","author":"Rastin","year":"2020","journal-title":"ACS Appl. Bio Mater."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"044104","DOI":"10.1088\/1758-5090\/aadf58","article-title":"3D bioprinting of a hyaluronan bioink through enzymatic-and visible light-crosslinking","volume":"10","author":"Petta","year":"2018","journal-title":"Biofabrication"},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Basara, G., Yue, X., and Zorlutuna, P. (2019). Dual Crosslinked Gelatin Methacryloyl Hydrogels for Photolithography and 3D Printing. Gels, 5.","DOI":"10.3390\/gels5030034"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"865","DOI":"10.1002\/jbm.a.36323","article-title":"Three-dimensional extrusion bioprinting of single- and double-network hydrogels containing dynamic covalent crosslinks","volume":"106","author":"Wang","year":"2018","journal-title":"J. Biomed. Mater. Res. Part A"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"eade7880","DOI":"10.1126\/sciadv.ade7880","article-title":"3D bioprinting of dynamic hydrogel bioinks enabled by small molecule modulators","volume":"9","author":"Hull","year":"2023","journal-title":"Sci. Adv."},{"key":"ref_22","doi-asserted-by":"crossref","unstructured":"Kaliampakou, C., Lagopati, N., Pavlatou, E.A., and Charitidis, C.A. (2023). Alginate-Gelatin Hydrogel Scaffolds; An Optimization of Post-Printing Treatment for Enhanced Degradation and Swelling Behavior. Gels, 9.","DOI":"10.3390\/gels9110857"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"e00468","DOI":"10.1016\/j.bprint.2026.e00468","article-title":"Improving alginate printability with alternative cationic pre-crosslinkers: Strontium-based bioinks and their impact on bioprinted cartilaginous tissues","volume":"55","author":"Steiner","year":"2026","journal-title":"Bioprinting"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1016\/j.biomaterials.2017.11.004","article-title":"Stress relaxing hyaluronic acid-collagen hydrogels promote cell spreading, fiber remodeling, and focal adhesion formation in 3D cell culture","volume":"154","author":"Lou","year":"2018","journal-title":"Biomaterials"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"101160","DOI":"10.1016\/j.mtbio.2024.101160","article-title":"Omnidirectional anisotropic embedded 3D bioprinting","volume":"27","author":"Shao","year":"2024","journal-title":"Mater. Today Bio"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"15667","DOI":"10.1021\/acsami.2c02069","article-title":"Structuring Hydrogel Cross-Link Density Using Hierarchical Filament 3D Printing","volume":"14","author":"Bayles","year":"2022","journal-title":"ACS Appl. Mater. Interfaces"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"e2303325","DOI":"10.1002\/adhm.202303325","article-title":"Embedded 3D Bioprinting of Collagen Inks into Microgel Baths to Control Hydrogel Microstructure and Cell Spreading","volume":"13","author":"Brunel","year":"2024","journal-title":"Adv. Healthc. Mater."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"6441","DOI":"10.1021\/acsbiomaterials.4c00819","article-title":"Rapidly Degrading Hydrogels to Support Biofabrication and 3D Bioprinting Using Cartilage Microtissues","volume":"10","author":"Kronemberger","year":"2024","journal-title":"ACS Biomater. Sci. Eng."},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"Liu, G., Wei, X.W., Li, J.L., Zhai, Y., Zhang, J.R., Jin, M., Guan, T.M., and Zhao, D.W. (2024). Hydrogel composition and mechanical stiffness of 3D bioprinted cell-loaded scaffolds promote cartilage regeneration. Front. Mater., 11.","DOI":"10.3389\/fmats.2024.1501505"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"e00196","DOI":"10.1016\/j.bprint.2022.e00196","article-title":"3D-bioprinted GelMA-gelatin-hydroxyapatite osteoblast-laden composite hydrogels for bone tissue engineering","volume":"26","author":"Allen","year":"2022","journal-title":"Bioprinting"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"e00105","DOI":"10.1016\/j.bprint.2020.e00105","article-title":"Development of 3D bioprinted GelMA-alginate hydrogels with tunable mechanical properties","volume":"21","author":"Aldana","year":"2021","journal-title":"Bioprinting"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"105365","DOI":"10.1016\/j.jmbbm.2022.105365","article-title":"Quantification of cell contractile behavior based on non-destructive macroscopic measurement of tension forces on bioprinted hydrogel","volume":"134","author":"Pragnere","year":"2022","journal-title":"J. Mech. Behav. Biomed. Mater."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"123174","DOI":"10.1016\/j.biomaterials.2025.123174","article-title":"Enhancing biofabrication: Shrink-resistant collagen-hyaluronan composite hydrogel for tissue engineering and 3D bioprinting applications","volume":"318","author":"Roshanbinfar","year":"2025","journal-title":"Biomaterials"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"2549","DOI":"10.1021\/acs.biomac.3c00057","article-title":"Direct 3D Bioprinting of Tough and Antifatigue Cell-Laden Constructs Enabled by a Self-Healing Hydrogel Bioink","volume":"24","author":"Liu","year":"2023","journal-title":"Biomacromolecules"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"e477","DOI":"10.1002\/agt2.477","article-title":"Extrusion bioprinting of elastin-containing bioactive double-network tough hydrogels for complex elastic tissue regeneration","volume":"5","author":"Wang","year":"2024","journal-title":"Aggregate"},{"key":"ref_36","doi-asserted-by":"crossref","unstructured":"Gharacheh, H., and Guvendiren, M. (2022). Cell-Laden Composite Hydrogel Bioinks with Human Bone Allograft Particles to Enhance Stem Cell Osteogenesis. Polymers, 14.","DOI":"10.3390\/polym14183788"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"e2502763","DOI":"10.1002\/adhm.202502763","article-title":"3D Bioprinting-Assisted Engineering of Stem Cell-Laden Hybrid Biopatches with Distinct Geometric Patterns Considering the Mechanical Characteristics of Regular and Irregular Connective Tissues","volume":"14","author":"Ahn","year":"2025","journal-title":"Adv. Healthc. Mater."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"2400157","DOI":"10.1002\/admt.202400157","article-title":"Hydrogel Ink for 3D Printing with High and Widely Tunable Mechanical Properties via the Salting-Out Effect","volume":"9","author":"Ko","year":"2024","journal-title":"Adv. Mater. Technol."},{"key":"ref_39","first-page":"104667","article-title":"Suspended bioprinting with in-situ elasticity monitoring using the assessment of shear wave phase velocity","volume":"99","author":"Kim","year":"2025","journal-title":"Addit. Manuf."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"109792","DOI":"10.1016\/j.matdes.2021.109792","article-title":"An imidazolium-based supramolecular gelator enhancing interlayer adhesion in 3D printed dual network hydrogels","volume":"206","author":"Zhou","year":"2021","journal-title":"Mater. Des."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"31002","DOI":"10.1021\/acsami.0c07468","article-title":"Chemically Coupled Interfacial Adhesion in Multimaterial Printing of Hydrogels and Elastomers","volume":"12","author":"Tian","year":"2020","journal-title":"ACS Appl. Mater. Interfaces"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"2401081","DOI":"10.1002\/admt.202401081","article-title":"High-Performance 3D Printed Mechanically Interlocked Soft-Hard Interfaces of Hydrogels and Polylactide","volume":"10","author":"Kunkels","year":"2025","journal-title":"Adv. Mater. Technol."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"e2400051","DOI":"10.1002\/adhm.202400051","article-title":"Bioprinting of Stable Bionic Interfaces Using Piezoresistive Hydrogel Organoelectronics","volume":"13","author":"Georgopoulou","year":"2024","journal-title":"Adv. Healthc. Mater."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"3158","DOI":"10.1039\/D2BM00223J","article-title":"A 3D printable perfused hydrogel vascular model to assay ultrasound-induced permeability","volume":"10","author":"Royse","year":"2022","journal-title":"Biomater. Sci."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"903","DOI":"10.1038\/s41598-025-29257-y","article-title":"Integration of multi-modal monitoring for dynamic control of large-scale 3D tissue bioreactors","volume":"16","author":"Chastagnier","year":"2026","journal-title":"Sci. Rep."},{"key":"ref_46","doi-asserted-by":"crossref","unstructured":"Figueiredo, L., Le Visage, C., Weiss, P., and Yang, J. (2020). Quantifying Oxygen Levels in 3D Bioprinted Cell-Laden Thick Constructs with Perfusable Microchannel Networks. Polymers, 12.","DOI":"10.3390\/polym12061260"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"240","DOI":"10.1007\/s42242-024-00281-7","article-title":"An oxygenating colloidal bioink for the engineering of biomimetic tissue constructs","volume":"7","author":"Jeong","year":"2024","journal-title":"Bio-Des. Manuf."},{"key":"ref_48","doi-asserted-by":"crossref","unstructured":"Barcelo, X., Eichholz, K.F., Garcia, O., and Kelly, D.J. (2022). Tuning the Degradation Rate of Alginate-Based Bioinks for Bioprinting Functional Cartilage Tissue. Biomedicines, 10.","DOI":"10.3390\/biomedicines10071621"},{"key":"ref_49","first-page":"365","article-title":"3D bio-printed proteinaceous bioactive scaffold loaded with dual growth factor enhanced chondrogenesis and in situ cartilage regeneration","volume":"46","author":"Shanto","year":"2025","journal-title":"Bioact. Mater."},{"key":"ref_50","doi-asserted-by":"crossref","unstructured":"Mazzoldi, E.L., Gaudenzi, G., Ginestra, P.S., Ceretti, E., and Giliani, S.C. (2024). Evaluating cells metabolic activity of bioinks for bioprinting: The role of cell-laden hydrogels and 3D printing on cell survival. Front. Bioeng. Biotechnol., 12.","DOI":"10.3389\/fbioe.2024.1450838"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"123965","DOI":"10.1016\/j.biomaterials.2025.123965","article-title":"Engineering bioactive fibrous constructs: Bioprinting stem cell-laden collagen-derived hydrogels with short collagen microfibers","volume":"329","author":"Weng","year":"2026","journal-title":"Biomaterials"},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"807","DOI":"10.1039\/D5BM00550G","article-title":"Design considerations for photoinitiator selection in cell-laden gelatin methacryloyl hydrogels","volume":"14","author":"Dogan","year":"2026","journal-title":"Biomater. Sci."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"213220","DOI":"10.1016\/j.bioadv.2022.213220","article-title":"Endotoxin contamination alters macrophage-cancer cell interaction and therapeutic efficacy in pre-clinical 3D in vitro models","volume":"144","author":"Heinrich","year":"2023","journal-title":"Biomater. Adv."},{"key":"ref_54","doi-asserted-by":"crossref","unstructured":"de Leeuw, A.M., Graf, R., Lim, P.J., Zhang, J., Schadli, G.N., Peterhans, S., Rohrbach, M., Giunta, C., Ruger, M., and Rubert, M. (2024). Physiological cell bioprinting density in human bone-derived cell-laden scaffolds enhances matrix mineralization rate and stiffness under dynamic loading. Front. Bioeng. Biotechnol., 12.","DOI":"10.3389\/fbioe.2024.1310289"},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"1437","DOI":"10.1038\/s42003-024-07106-4","article-title":"Unveiling the impact of hypodermis on gene expression for advancing bioprinted full-thickness 3D skin models","volume":"7","author":"Avelino","year":"2024","journal-title":"Commun. Biol."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"19987","DOI":"10.1038\/s41598-019-56117-3","article-title":"3D bioprinting via an in situ crosslinking technique towards engineering cartilage tissue","volume":"9","author":"Galarraga","year":"2019","journal-title":"Sci. Rep."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"50","DOI":"10.1007\/s10856-026-07024-3","article-title":"Development of biodegradable methacrylated guar gum 3D bioprinting bioinks for stem cell delivery and cartilage tissue engineering","volume":"37","author":"Shang","year":"2026","journal-title":"J. Mater. Sci. Mater. Med."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"110864","DOI":"10.1016\/j.compositesb.2023.110864","article-title":"3D printing of high-strength photo-crosslinking flaxseed gum bioink for cartilage regeneration","volume":"263","author":"Shu","year":"2023","journal-title":"Compos. Part B\u2014Eng."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"e2202638","DOI":"10.1002\/advs.202202638","article-title":"Machine Learning Reveals a General Understanding of Printability in Formulations Based on Rheology Additives","volume":"9","author":"Nadernezhad","year":"2022","journal-title":"Adv. Sci."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"0197","DOI":"10.34133\/research.0197","article-title":"Machine Learning in Predicting Printable Biomaterial Formulations for Direct Ink Writing","volume":"6","author":"Chen","year":"2023","journal-title":"Research"},{"key":"ref_61","doi-asserted-by":"crossref","unstructured":"Sarah, R., Schimmelpfennig, K., Rohauer, R., Lewis, C.L., Limon, S.M., and Habib, A. (2025). Characterization and Machine Learning-Driven Property Prediction of a Novel Hybrid Hydrogel Bioink Considering Extrusion-Based 3D Bioprinting. Gels, 11.","DOI":"10.3390\/gels11010045"},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"741","DOI":"10.18063\/ijb.741","article-title":"A computational model of cell viability and proliferation of extrusion-based 3D-bioprinted constructs during tissue maturation process","volume":"9","author":"Gironi","year":"2023","journal-title":"Int. J. Bioprint."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"116044","DOI":"10.1016\/j.matdes.2026.116044","article-title":"Analysis and modeling of the contraction phenomenon in the post-processing of bioprinted bone","volume":"266","author":"Katoozian","year":"2026","journal-title":"Mater. Des."},{"key":"ref_64","unstructured":"U.S. Food and Drug Administration (2011). Potency Tests for Cellular and Gene Therapy Products: Final Guidance for Industry."},{"key":"ref_65","unstructured":"U.S. Food and Drug Administration (2023). Potency Assurance for Cellular and Gene Therapy Products: Draft Guidance for Industry."},{"key":"ref_66","unstructured":"European Medicines Agency (2025). Guideline on Quality, Non-Clinical and Clinical Requirements for Investigational Advanced Therapy Medicinal Products in Clinical Trials, European Medicines Agency. EMA\/CAT\/22473\/2025."},{"key":"ref_67","unstructured":"(2020). General Requirements of Tissue-Engineered Medical Products (Standard No. ISO\/TS 21560:2020)."},{"key":"ref_68","unstructured":"Maddalon, A., Piergiovanni, M., Urb\u00e1n Lopez, P., Armas, F., Barrero, J., Colpo, P., Kourti, N., Zimmer, S., Dalier, C., and Ogbonna, J. (2026). Putting Science into Standards\u20143D Bioprinting: Towards Standards in Biomedicine, Publications Office of the European Union."}],"container-title":["Gels"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2310-2861\/12\/8\/742\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T13:33:31Z","timestamp":1787146411000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2310-2861\/12\/8\/742"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,8,19]]},"references-count":68,"journal-issue":{"issue":"8","published-online":{"date-parts":[[2026,8]]}},"alternative-id":["gels12080742"],"URL":"https:\/\/doi.org\/10.3390\/gels12080742","relation":{},"ISSN":["2310-2861"],"issn-type":[{"value":"2310-2861","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,8,19]]}}}