{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T21:01:39Z","timestamp":1792789299702,"version":"4.5.0"},"reference-count":107,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T00:00:00Z","timestamp":1789516800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Piedmont Region project IPER","id":[{"id":"https:\/\/ror.org\/04fbf9935","id-type":"ROR","asserted-by":"crossref"}]},{"id":[{"id":"https:\/\/ror.org\/04fbf9935","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Gels"],"abstract":"<jats:p>Gelatin is a natural biopolymer derived from collagen and is widely employed in biomedical applications because of its biocompatibility, biodegradability, low toxicity, water solubility, and intrinsic thermo-responsive gelation behavior. Owing to the presence of bioactive motifs and its ability to form hydrogels under mild conditions, gelatin has emerged as a promising material for tissue engineering, drug delivery, and in vitro modeling. However, the poor mechanical stability and rapid dissolution of native gelatin under physiological conditions limit its direct use in advanced biomedical systems. To overcome these drawbacks, several chemical modification strategies have been developed, among which gelatin methacryloyl (GelMA) is one of the most investigated derivatives. GelMA combines the biological advantages of gelatin with photo-crosslinkable methacryloyl groups, enabling the fabrication of stable hydrogels with tunable mechanical, rheological, and degradation properties. This review adopts a source-to-performance perspective, systematically examining how gelatin origin, processing history, molecular characteristics, and Bloom strength may influence GelMA functionalization and subsequent hydrogel network formation. Particular attention is given to Type A and Type B gelatin, while recognizing that this classification does not fully capture the variability in the gelatin precursor. The review critically discusses how precursor characteristics interact with key synthesis and formulation parameters, including the degree of substitution\/functionalization (DS\/DoF), polymer concentration, photoinitiator content, and photo-crosslinking conditions, ultimately affecting network formation and the mechanical, rheological, swelling, porosity, degradation, and biological properties of GelMA hydrogels. This interconnected view highlights how variability introduced at the precursor level may propagate through functionalization and crosslinking, contributing to differences in GelMA performance and limiting direct comparison among reported formulations. The implications of these material-dependent properties are examined across three major biomedical application areas: tissue engineering, controlled drug delivery, and physiologically relevant three-dimensional in vitro models. Finally, current challenges and emerging opportunities related to GelMA standardization, biofabrication, multifunctional hydrogel design, personalized medicine, and clinical translation are considered. Overall, GelMA is presented not simply as a versatile biomaterial, but as a tunable protein-derived platform whose performance depends on the interconnected effects of precursor characteristics, functionalization, formulation, and network formation.<\/jats:p>","DOI":"10.3390\/gels12090844","type":"journal-article","created":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T07:58:59Z","timestamp":1789545539000},"page":"844","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["From\u00a0Gelatin to GelMA: Versatility, Challenges, and Biomedical Applications of GelMA Hydrogels"],"prefix":"10.3390","volume":"12","author":[{"given":"Federica","family":"Gemignani","sequence":"first","affiliation":[{"name":"Dipartimento di Scienza Applicata e Tecnologia, PolitoBIOMed Lab, Politecnico di Torino, 10129 Turin, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8950-9054","authenticated-orcid":false,"given":"Giulia","family":"Mesiano","sequence":"additional","affiliation":[{"name":"Dipartimento di Scienza Applicata e Tecnologia, PolitoBIOMed Lab, Politecnico di Torino, 10129 Turin, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-6977-4076","authenticated-orcid":false,"given":"Pompeo Marco","family":"Gaudiosi","sequence":"additional","affiliation":[{"name":"Dipartimento di Scienza Applicata e Tecnologia, PolitoBIOMed Lab, Politecnico di Torino, 10129 Turin, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4991-9459","authenticated-orcid":false,"given":"Fabrizio Candido","family":"Pirri","sequence":"additional","affiliation":[{"name":"Dipartimento di Scienza Applicata e Tecnologia, PolitoBIOMed Lab, Politecnico di Torino, 10129 Turin, Italy"},{"name":"Center for Sustainable Futures, Istituto Italiano di Tecnologia, 10144 Turin, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6543-6038","authenticated-orcid":false,"given":"Francesca","family":"Frascella","sequence":"additional","affiliation":[{"name":"Dipartimento di Scienza Applicata e Tecnologia, PolitoBIOMed Lab, Politecnico di Torino, 10129 Turin, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,9,16]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Ho, T.C., Chang, C.C., Chan, H.P., Chung, T.W., Shu, C.W., Chuang, K.P., Duh, T.H., Yang, M.H., and Tyan, Y.C. (2022). Hydrogels: Properties and Applications in Biomedicine. Molecules, 27.","DOI":"10.3390\/molecules27092902"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"042008","DOI":"10.1088\/1748-605X\/ad4df7","article-title":"Unveiling the Versatility of Gelatin Methacryloyl Hydrogels: A Comprehensive Journey into Biomedical Applications","volume":"19","author":"Pramanik","year":"2024","journal-title":"Biomed. Mater."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"2156","DOI":"10.1021\/acs.biomac.3c01271","article-title":"Gelatin Methacryloyl (GelMA)-Based Biomaterial Inks: Process Science for 3D\/4D Printing and Current Status","volume":"25","author":"Das","year":"2024","journal-title":"Biomacromolecules"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"164","DOI":"10.1089\/ten.teb.2019.0256","article-title":"Engineering and Functionalization of Gelatin Biomaterials: From Cell Culture to Medical Applications","volume":"26","author":"Bello","year":"2020","journal-title":"Tissue Eng. Part B Rev."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"127143","DOI":"10.1016\/j.ijbiomac.2023.127143","article-title":"Advancements in Gelatin-Based Hydrogel Systems for Biomedical Applications: A State-of-the-Art Review","volume":"253","author":"Mohanto","year":"2023","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"184","DOI":"10.1039\/C9PY01021A","article-title":"Hydrogel Scaffolds for Tissue Engineering: The Importance of Polymer Choice","volume":"11","author":"Spicer","year":"2020","journal-title":"Polym. Chem."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"254","DOI":"10.1016\/j.biomaterials.2015.08.045","article-title":"Synthesis, Properties, and Biomedical Applications of Gelatin Methacryloyl (GelMA) Hydrogels","volume":"73","author":"Yue","year":"2015","journal-title":"Biomaterials"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"6863","DOI":"10.1038\/s41598-019-42186-x","article-title":"Gelatin Methacryloyl and Its Hydrogels with an Exceptional Degree of Controllability and Batch-to-Batch Consistency","volume":"9","author":"Zhu","year":"2019","journal-title":"Sci. Rep."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"191","DOI":"10.1007\/s44174-022-00023-2","article-title":"Hybrid Hydrogels Based on Methacrylate-Functionalized Gelatin (GelMA) and Synthetic Polymers","volume":"1","author":"Liang","year":"2023","journal-title":"Biomed. Mater. Devices"},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"Lee, B.H., Lum, N., Seow, L.Y., Lim, P.Q., and Tan, L.P. (2016). Synthesis and Characterization of Types A and B Gelatin Methacryloyl for Bioink Applications. Materials, 9.","DOI":"10.3390\/ma9100797"},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Nguyen, A.K., Goering, P.L., Elespuru, R.K., Das, S.S., and Narayan, R.J. (2020). The Photoinitiator Lithium Phenyl (2,4,6-Trimethylbenzoyl) Phosphinate with Exposure to 405 Nm Light Is Cytotoxic to Mammalian Cells but Not Mutagenic in Bacterial Reverse Mutation Assays. Polymers, 12.","DOI":"10.3390\/polym12071489"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"23276","DOI":"10.1038\/s41598-021-02830-x","article-title":"Systematic Optimization of Visible Light-Induced Crosslinking Conditions of Gelatin Methacryloyl (GelMA)","volume":"11","author":"Sharifi","year":"2021","journal-title":"Sci. Rep."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"e1900098","DOI":"10.1002\/mabi.201900098","article-title":"Visible Light Cross-Linking of Gelatin Hydrogels Offers an Enhanced Cell Microenvironment with Improved Light Penetration Depth","volume":"19","author":"Lim","year":"2019","journal-title":"Macromol. Biosci."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"e00376","DOI":"10.1002\/marc.202500376","article-title":"Effect of Photoinitiation Process on Photo-Crosslinking of Gelatin Methacryloyl Hydrogel Networks","volume":"46","author":"Duymaz","year":"2025","journal-title":"Macromol. Rapid Commun."},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"Al-Nimry, S., Dayah, A.A., Hasan, I., and Daghmash, R. (2021). Cosmetic, Biomedical and Pharmaceutical Applications of Fish Gelatin\/Hydrolysates. Mar. Drugs, 19.","DOI":"10.3390\/md19030145"},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Derkach, S.R., Voron\u2019ko, N.G., Kuchina, Y.A., and Kolotova, D.S. (2020). Modified Fish Gelatin as an Alternative to Mammalian Gelatin in Modern Food Technologies. Polymers, 12.","DOI":"10.3390\/polym12123051"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"5666","DOI":"10.7150\/thno.109186","article-title":"Fish-Derived Biomaterials for Tissue Engineering: Advances in Scaffold Fabrication and Applications in Regenerative Medicine and Cancer Therapy","volume":"15","author":"Jo","year":"2025","journal-title":"Theranostics"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"481","DOI":"10.1080\/20014091091904","article-title":"Gelatin: A Valuable Protein for Food and Pharmaceutical Industries: Review","volume":"41","author":"Djagny","year":"2001","journal-title":"Crit. Rev. Food Sci. Nutr."},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Campiglio, C.E., Negrini, N.C., Far\u00e8, S., and Draghi, L. (2019). Cross-Linking Strategies for Electrospun Gelatin Scaffolds. Materials, 12.","DOI":"10.3390\/ma12152476"},{"key":"ref_20","first-page":"267","article-title":"Multifunctional GelMA Platforms with Nanomaterials for Advanced Tissue Therapeutics","volume":"8","author":"Kurian","year":"2022","journal-title":"Bioact. Mater."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"e2300395","DOI":"10.1002\/adhm.202300395","article-title":"Gelatin Methacryloyl Hydrogel, from Standardization, Performance, to Biomedical Application","volume":"12","author":"He","year":"2023","journal-title":"Adv. Healthc. Mater."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"e1800168","DOI":"10.1002\/mabi.201800168","article-title":"Beyond the Modification Degree: Impact of Raw Material on Physicochemical Properties of Gelatin Type A and Type B Methacryloyls","volume":"18","author":"Sewald","year":"2018","journal-title":"Macromol. Biosci."},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"Aljaber, M.B., Verisqa, F., Keskin-Erdogan, Z., Patel, K.D., Chau, D.Y.S., and Knowles, J.C. (2023). Influence of Gelatin Source and Bloom Number on Gelatin Methacryloyl Hydrogels Mechanical and Biological Properties for Muscle Regeneration. Biomolecules, 13.","DOI":"10.3390\/biom13050811"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"106509","DOI":"10.1016\/j.jmbbm.2024.106509","article-title":"Evaluation of Gelatin Bloom Strength on Gelatin Methacryloyl Hydrogel Properties","volume":"154","author":"Zambuto","year":"2024","journal-title":"J. Mech. Behav. Biomed. Mater."},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"Gaglio, C.G., Baruffaldi, D., Pirri, C.F., Napione, L., and Frascella, F. (2024). GelMA Synthesis and Sources Comparison for 3D Multimaterial Bioprinting. Front. Bioeng. Biotechnol., 12.","DOI":"10.3389\/fbioe.2024.1383010"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"20","DOI":"10.1111\/jocd.12450","article-title":"Collagen: A Review on Its Sources and Potential Cosmetic Applications","volume":"17","year":"2018","journal-title":"J. Cosmet. Dermatol."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"1813","DOI":"10.1016\/j.foodhyd.2011.02.007","article-title":"Functional and Bioactive Properties of Collagen and Gelatin from Alternative Sources: A Review","volume":"25","author":"Gimenez","year":"2011","journal-title":"Food Hydrocoll."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"260","DOI":"10.1016\/j.tifs.2019.02.048","article-title":"Fish Gelatin Modifications: A Comprehensive Review","volume":"86","author":"Huang","year":"2019","journal-title":"Trends Food Sci. Technol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"2316","DOI":"10.1002\/pol.20230141","article-title":"Investigation of the Influence of PH on the Properties and Morphology of Gelatin Hydrogels","volume":"61","author":"Goudie","year":"2023","journal-title":"J. Polym. Sci."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"2212","DOI":"10.1002\/jbm.a.37206","article-title":"Utilization of GelMA with Phosphate Glass Fibers for Glial Cell Alignment","volume":"109","author":"Patel","year":"2021","journal-title":"J. Biomed. Mater. Res. A"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"601","DOI":"10.1016\/j.ijbiomac.2022.07.168","article-title":"Preparation, Properties, and Applications of Gelatin-Based Hydrogels (GHs) in the Environmental, Technological, and Biomedical Sectors","volume":"218","author":"Mushtaq","year":"2022","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"109026","DOI":"10.1016\/j.polymertesting.2025.109026","article-title":"Viscoelastic Interpretation of AFM Nanoindentation for Predicting Nanoscale Stiffness in Soft Biomaterials","volume":"153","author":"Minopoli","year":"2025","journal-title":"Polym. Test."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"129781","DOI":"10.1016\/j.polymer.2026.129781","article-title":"Effect of Source and Reaction Conditions on the Structure and Rheological Properties of Gelatin Methacryloyl","volume":"350","author":"Borrelli","year":"2026","journal-title":"Polymer"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"30","DOI":"10.1016\/j.ijpharm.2016.06.129","article-title":"On the Importance of Bloom Number of Gelatin to the Development of Biodegradable in Situ Gelling Copolymers for Intracameral Drug Delivery","volume":"511","author":"Chou","year":"2016","journal-title":"Int. J. Pharm."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"110899","DOI":"10.1016\/j.lwt.2021.110899","article-title":"Fish Gelatin as an Alternative to Mammalian Gelatin for Food Industry: A Meta-Analysis","volume":"141","author":"Nitsuwat","year":"2021","journal-title":"LWT"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"e2000183","DOI":"10.1002\/mabi.202000183","article-title":"Rheological Properties of Coordinated Physical Gelation and Chemical Crosslinking in Gelatin Methacryloyl (GelMA) Hydrogels","volume":"20","author":"Young","year":"2020","journal-title":"Macromol. Biosci."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"107535","DOI":"10.1016\/j.foodhyd.2022.107535","article-title":"Long-Term Storage Stability of Type A and Type B Gelatin Gels: The Effect of Bloom Strength and Co-Solutes","volume":"127","author":"Baydin","year":"2022","journal-title":"Food Hydrocoll."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"843","DOI":"10.1039\/C8BM01246F","article-title":"Advances in Crosslinking Strategies of Biomedical Hydrogels","volume":"7","author":"Hu","year":"2019","journal-title":"Biomater. Sci."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1021\/bm990017d","article-title":"Structural and Rheological Properties of Methacrylamide Modified Gelatin Hydrogels","volume":"1","author":"Bogdanov","year":"2000","journal-title":"Biomacromolecules"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"2928","DOI":"10.1021\/acs.biomac.3c00302","article-title":"Structure and Properties of Gelatin Methacryloyl (GelMA) Synthesized in Different Reaction Systems","volume":"24","author":"Chen","year":"2023","journal-title":"Biomacromolecules"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"149458","DOI":"10.1016\/j.ijbiomac.2025.149458","article-title":"Exploring the Impact of Gelatin Bloom Strength and Degree of Functionalization in Gelatin Methacryloyl Bioinks for Optimum Digital Light Processing and Extrusion 3D Bioprinting","volume":"338","author":"Ajgaonkar","year":"2026","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"2300085","DOI":"10.1002\/macp.202300085","article-title":"Photoluminescence Studies of DoF Effect on the Photopolymerization Reaction of a GelMA-Based Monomers for Tissue Engineering","volume":"225","year":"2024","journal-title":"Macromol. Chem. Phys."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"31036","DOI":"10.1038\/srep31036","article-title":"Precise Tuning of Facile One-Pot Gelatin Methacryloyl (GelMA) Synthesis","volume":"6","author":"Shirahama","year":"2016","journal-title":"Sci. Rep."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"5496","DOI":"10.1039\/D3MA00715D","article-title":"An Insight into Synthesis, Properties and Applications of Gelatin Methacryloyl Hydrogel for 3D Bioprinting","volume":"4","author":"Ghosh","year":"2023","journal-title":"Mater. Adv."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"6211","DOI":"10.1007\/s00216-020-02792-5","article-title":"Quantification of Fractional and Absolute Functionalization of Gelatin Hydrogels by Optimized Ninhydrin Assay and 1H NMR","volume":"412","author":"Zatorski","year":"2020","journal-title":"Anal. Bioanal. Chem."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"6702","DOI":"10.1016\/j.biomaterials.2009.08.055","article-title":"Photoinitiated Polymerization of PEG-Diacrylate with Lithium Phenyl-2,4,6-Trimethylbenzoylphosphinate: Polymerization Rate and Cytocompatibility","volume":"30","author":"Fairbanks","year":"2009","journal-title":"Biomaterials"},{"key":"ref_47","doi-asserted-by":"crossref","unstructured":"Wang, X., Ao, Q., Tian, X., Fan, J., Tong, H., Hou, W., and Bai, S. (2017). Gelatin-Based Hydrogels for Organ 3D Bioprinting. Polymers, 9.","DOI":"10.3390\/polym9090401"},{"key":"ref_48","doi-asserted-by":"crossref","unstructured":"Pepelanova, I., Kruppa, K., Scheper, T., and Lavrentieva, A. (2018). Gelatin-Methacryloyl (GelMA) Hydrogels with Defined Degree of Functionalization as a Versatile Toolkit for 3D Cell Culture and Extrusion Bioprinting. Bioengineering, 5.","DOI":"10.3390\/bioengineering5030055"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"225","DOI":"10.25122\/jml-2019-0032","article-title":"Tissue Engineering; Current Status & Futuristic Scope","volume":"2019","author":"Sharma","year":"2019","journal-title":"J. Med. Life"},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"e14533","DOI":"10.1111\/iwj.14533","article-title":"Recent Advances on 3D-Bioprinted Gelatin Methacrylate Hydrogels for Tissue Engineering in Wound Healing: A Review of Current Applications and Future Prospects","volume":"21","author":"Wang","year":"2024","journal-title":"Int. Wound J."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"807","DOI":"10.1039\/D5BM00550G","article-title":"Design Considerations for Photoinitiator Selection in Cell-Laden Gelatin Methacryloyl Hydrogels","volume":"14","author":"Dogan","year":"2026","journal-title":"Biomater. Sci."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"055021","DOI":"10.1088\/1748-605X\/ab954e","article-title":"Effects of Irgacure 2959 and Lithium Phenyl-2,4,6-Trimethylbenzoylphosphinate on Cell Viability, Physical Properties, and Microstructure in 3D Bioprinting of Vascular-like Constructs","volume":"15","author":"Xu","year":"2020","journal-title":"Biomed. Mater."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"5536","DOI":"10.1016\/j.biomaterials.2010.03.064","article-title":"Cell-Laden Microengineered Gelatin Methacrylate Hydrogels","volume":"31","author":"Nichol","year":"2010","journal-title":"Biomaterials"},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"125316","DOI":"10.1016\/j.polymer.2022.125316","article-title":"Cross-Evaluation of Stiffness Measurement Methods for Hydrogels","volume":"258","author":"Richbourg","year":"2022","journal-title":"Polymer"},{"key":"ref_55","doi-asserted-by":"crossref","unstructured":"Sun, M., Sun, X., Wang, Z., Guo, S., Yu, G., and Yang, H. (2018). Synthesis and Properties of Gelatin Methacryloyl (GelMA) Hydrogels and Their Recent Applications in Load-Bearing Tissue. Polymers, 10.","DOI":"10.3390\/polym10111290"},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"2027","DOI":"10.1002\/adfm.201101662","article-title":"Functional Human Vascular Network Generated in Photocrosslinkable Gelatin Methacrylate Hydrogels","volume":"22","author":"Chen","year":"2012","journal-title":"Adv. Funct. Mater."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"146485","DOI":"10.1016\/j.ijbiomac.2025.146485","article-title":"Research Progress on Stiffness Controllable Scaffolds Based on Gelatin Methacryloyl Hydrogels for Tissue Repair and Reconstruction","volume":"321","author":"Liu","year":"2025","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"394","DOI":"10.1016\/j.tibtech.2016.01.002","article-title":"Gelatin-Methacryloyl Hydrogels: Towards Biofabrication-Based Tissue Repair","volume":"34","author":"Klotz","year":"2016","journal-title":"Trends Biotechnol."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"112938","DOI":"10.1016\/j.ijsolstr.2024.112938","article-title":"Triaxial Mechanical Characterization of Ultrasoft 3D Support Bath-Based Bioprinted Tubular GelMA Constructs","volume":"301","author":"Torza","year":"2024","journal-title":"Int. J. Solids Struct."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"e2409355","DOI":"10.1002\/adma.202409355","article-title":"Multi-Material Volumetric Bioprinting and Plug-and-Play Suspension Bath Biofabrication via Bioresin Molecular Weight Tuning and via Multiwavelength Alignment Optics","volume":"37","author":"Ribezzi","year":"2025","journal-title":"Adv. Mater."},{"key":"ref_61","first-page":"47","article-title":"Biomedical Applications of Gelatin Methacryloyl Hydrogels","volume":"2","author":"Piao","year":"2021","journal-title":"Eng. Regen."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"91","DOI":"10.1016\/j.ijbiomac.2022.08.171","article-title":"Recent Advances on Gelatin Methacrylate Hydrogels with Controlled Microstructures for Tissue Engineering","volume":"221","author":"Zhang","year":"2022","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"102111","DOI":"10.1016\/j.mtchem.2024.102111","article-title":"GelMA Hydrogel: A Game-Changer in 3D Tumor Modeling","volume":"38","author":"Jiao","year":"2024","journal-title":"Mater. Today Chem."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"523","DOI":"10.1007\/s13770-023-00522-3","article-title":"Tissue-Engineered 3D In Vitro Disease Models for High-Throughput Drug Screening","volume":"20","author":"Huskin","year":"2023","journal-title":"Tissue Eng. Regen. Med."},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"108","DOI":"10.1002\/adhm.201500005","article-title":"Photocrosslinkable Gelatin Hydrogel for Epidermal Tissue Engineering","volume":"5","author":"Zhao","year":"2016","journal-title":"Adv. Healthc. Mater."},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"1802","DOI":"10.1021\/acsabm.8b00663","article-title":"Silver-Nanoparticle-Entrapped Soft GelMA Gels as Prospective Scaffolds for Wound Healing","volume":"2","author":"Jahan","year":"2019","journal-title":"ACS Appl. Bio Mater."},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"146246","DOI":"10.1016\/j.cej.2023.146246","article-title":"Multifunctional MMP9-Responsive Silicasomes-GelMA Hydrogels with Bacteria-Targeting Capability and Tissue Restoration Function for Chronic Wound Infection","volume":"475","author":"Chen","year":"2023","journal-title":"Chem. Eng. J."},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"e10373","DOI":"10.1002\/btm2.10373","article-title":"Bio-functional Hydrogel with Antibacterial and Anti-inflammatory Dual Properties to Combat with Burn Wound Infection","volume":"8","author":"Xiong","year":"2023","journal-title":"Bioeng. Transl. Med."},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"918","DOI":"10.1016\/j.ijbiomac.2021.05.040","article-title":"Synthesis and Characterization of C2C12-Laden Gelatin Methacryloyl (GelMA) from Marine and Mammalian Sources","volume":"183","author":"Elkhoury","year":"2021","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_70","doi-asserted-by":"crossref","unstructured":"Wasy\u0142eczko, M., Sikorska, W., and Chwojnowski, A. (2020). Review of Synthetic and Hybrid Scaffolds in Cartilage Tissue Engineering. Membranes, 10.","DOI":"10.3390\/membranes10110348"},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"6933","DOI":"10.1038\/ncomms7933","article-title":"Reinforcement of Hydrogels Using Three-Dimensionally Printed Microfibres","volume":"6","author":"Visser","year":"2015","journal-title":"Nat. Commun."},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"045006","DOI":"10.1088\/1748-605X\/ab401f","article-title":"Hydrogel to Guide Chondrogenesis versus Osteogenesis of Mesenchymal Stem Cells for Fabrication of Cartilaginous Tissues","volume":"15","author":"Chen","year":"2020","journal-title":"Biomed. Mater."},{"key":"ref_73","doi-asserted-by":"crossref","first-page":"109659","DOI":"10.1016\/j.cbi.2021.109659","article-title":"Fluoride Regulates Chondrocyte Proliferation and Autophagy via PI3K\/AKT\/MTOR Signaling Pathway","volume":"349","author":"Ma","year":"2021","journal-title":"Chem. Biol. Interact."},{"key":"ref_74","doi-asserted-by":"crossref","first-page":"e2406942","DOI":"10.1002\/advs.202406942","article-title":"Microneedle-Delivered PDA@Exo for Multifaceted Osteoarthritis Treatment via PI3K-Akt-MTOR Pathway","volume":"11","author":"Li","year":"2024","journal-title":"Adv. Sci."},{"key":"ref_75","doi-asserted-by":"crossref","unstructured":"Machado, I., Marques, C.F., Martins, E., Alves, A.L., Reis, R.L., and Silva, T.H. (2023). Marine Gelatin-Methacryloyl-Based Hydrogels as Cell Templates for Cartilage Tissue Engineering. Polymers, 15.","DOI":"10.3390\/polym15071674"},{"key":"ref_76","doi-asserted-by":"crossref","unstructured":"Wang, Y., Ma, M., Wang, J., Zhang, W., Lu, W., Gao, Y., Zhang, B., and Guo, Y. (2018). Development of a Photo-Crosslinking, Biodegradable GelMA\/PEGDA Hydrogel for Guided Bone Regeneration Materials. Materials, 11.","DOI":"10.3390\/ma11081345"},{"key":"ref_77","doi-asserted-by":"crossref","first-page":"2809","DOI":"10.1002\/adfm.201504943","article-title":"Injectable Stem Cell-Laden Photocrosslinkable Microspheres Fabricated Using Microfluidics for Rapid Generation of Osteogenic Tissue Constructs","volume":"26","author":"Zhao","year":"2016","journal-title":"Adv. Funct. Mater."},{"key":"ref_78","doi-asserted-by":"crossref","first-page":"3270","DOI":"10.1021\/acsbiomaterials.0c00443","article-title":"Biomimetic Gelatin Methacrylate\/Nano Fish Bone Hybrid Hydrogel for Bone Regeneration via Osteoimmunomodulation","volume":"6","author":"Huang","year":"2020","journal-title":"ACS Biomater. Sci. Eng."},{"key":"ref_79","doi-asserted-by":"crossref","unstructured":"Alves, A.L., Carvalho, A.C., Machado, I., Diogo, G.S., Fernandes, E.M., Castro, V.I.B., Pires, R.A., V\u00e1zquez, J.A., P\u00e9rez-Mart\u00edn, R.I., and Alaminos, M. (2023). Cell-Laden Marine Gelatin Methacryloyl Hydrogels Enriched with Ascorbic Acid for Corneal Stroma Regeneration. Bioengineering, 10.","DOI":"10.3390\/bioengineering10010062"},{"key":"ref_80","doi-asserted-by":"crossref","first-page":"461","DOI":"10.1039\/D2BM01200F","article-title":"Perfusable Cell-Laden Matrices to Guide Patterning of Vascularization in Vivo","volume":"11","author":"Parkhideh","year":"2022","journal-title":"Biomater. Sci."},{"key":"ref_81","first-page":"55130","article-title":"Matrix Stiffness of GelMA Hydrogels Regulates Lymphatic Endothelial Cells toward Enhanced Lymphangiogenesis","volume":"16","author":"Qin","year":"2024","journal-title":"ACS Appl. Mater. Interfaces"},{"key":"ref_82","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.biomaterials.2015.12.006","article-title":"Intra-Articular Delivery of Sinomenium Encapsulated by Chitosan Microspheres and Photo-Crosslinked GelMA Hydrogel Ameliorates Osteoarthritis by Effectively Regulating Autophagy","volume":"81","author":"Chen","year":"2016","journal-title":"Biomaterials"},{"key":"ref_83","doi-asserted-by":"crossref","first-page":"106039","DOI":"10.1016\/j.isci.2023.106039","article-title":"IScience Stereolithography Apparatus and Digital Light Processing-Based 3D Bioprinting for Tissue Fabrication","volume":"26","author":"Li","year":"2023","journal-title":"iScience"},{"key":"ref_84","doi-asserted-by":"crossref","first-page":"025011","DOI":"10.1088\/1758-5090\/adab27","article-title":"Digital Light Processing 3D Bioprinting of Biomimetic Corneal Stroma Equivalent Using Gelatin Methacryloyl and Oxidized Carboxymethylcellulose Interpenetrating Network Hydrogel","volume":"17","author":"Chand","year":"2025","journal-title":"Biofabrication"},{"key":"ref_85","doi-asserted-by":"crossref","first-page":"106553","DOI":"10.1016\/j.jmbbm.2024.106553","article-title":"SLA-3d Printed Building and Characteristics of GelMA\/HAP Biomaterials with Gradient Porous Structure","volume":"155","author":"Chen","year":"2024","journal-title":"J. Mech. Behav. Biomed. Mater."},{"key":"ref_86","doi-asserted-by":"crossref","unstructured":"Fu, H., and Yu, B. (2024). 3D Micro\/Nano Hydrogel Structures Fabricated by Two-Photon Polymerization for Biomedical Applications. Front. Bioeng. Biotechnol., 12.","DOI":"10.3389\/fbioe.2024.1339450"},{"key":"ref_87","doi-asserted-by":"crossref","first-page":"e2000016","DOI":"10.1002\/adbi.202000016","article-title":"Bioinspired Precision Engineering of Three-Dimensional Epithelial Stem Cell Microniches","volume":"4","author":"Prina","year":"2020","journal-title":"Adv. Biosyst."},{"key":"ref_88","doi-asserted-by":"crossref","unstructured":"Vigata, M., Meinert, C., Pahoff, S., Bock, N., and Hutmacher, D.W. (2020). Gelatin Methacryloyl Hydrogels Control the Localized Delivery of Albumin-Bound Paclitaxel. Polymers, 12.","DOI":"10.3390\/polym12020501"},{"key":"ref_89","doi-asserted-by":"crossref","unstructured":"Vigata, M., O\u2019connell, C.D., Cometta, S., Hutmacher, D.W., Meinert, C., and Bock, N. (2021). Gelatin Methacryloyl Hydrogels for the Localized Delivery of Cefazolin. Polymers, 13.","DOI":"10.3390\/polym13223960"},{"key":"ref_90","doi-asserted-by":"crossref","unstructured":"Vigata, M., Meinert, C., Bock, N., Dargaville, B.L., and Hutmacher, D.W. (2021). Deciphering the Molecular Mechanism of Water Interaction with Gelatin Methacryloyl Hydrogels: Role of Ionic Strength, Ph, Drug Loading and Hydrogel Network Characteristics. Biomedicines, 9.","DOI":"10.3390\/biomedicines9050574"},{"key":"ref_91","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1016\/j.actbio.2020.03.015","article-title":"A Liposome\/Gelatin Methacrylate Nanocomposite Hydrogel System for Delivery of Stromal Cell-Derived Factor-1\u03b1 and Stimulation of Cell Migration","volume":"108","author":"Yu","year":"2020","journal-title":"Acta Biomater."},{"key":"ref_92","doi-asserted-by":"crossref","unstructured":"Kang, M.G., Lee, M.Y., Cha, J.M., Lee, J.K., Lee, S.C., Kim, J., Hwang, Y.S., and Bae, H. (2019). Nanogels Derived from Fish Gelatin: Application to Drug Delivery System. Mar. Drugs, 17.","DOI":"10.3390\/md17040246"},{"key":"ref_93","doi-asserted-by":"crossref","unstructured":"Hu, Q., Williams, S.L., Palladino, A., and Ecker, M. (2024). Screening of MMP-13 Inhibitors Using a GelMA-Alginate Interpenetrating Network Hydrogel-Based Model Mimicking Cytokine-Induced Key Features of Osteoarthritis In Vitro. Polymers, 16.","DOI":"10.3390\/polym16111572"},{"key":"ref_94","doi-asserted-by":"crossref","unstructured":"Cao, H., Duan, L., Zhang, Y., Cao, J., and Zhang, K. (2021). Current Hydrogel Advances in Physicochemical and Biological Response-Driven Biomedical Application Diversity. Signal Transduct. Target. Ther., 6.","DOI":"10.1038\/s41392-021-00830-x"},{"key":"ref_95","doi-asserted-by":"crossref","first-page":"8214","DOI":"10.1038\/s41598-024-81533-5","article-title":"GelMA as Scaffold Material for Epithelial Cells to Emulate the Small Intestinal Microenvironment","volume":"15","author":"Roegiers","year":"2025","journal-title":"Sci. Rep."},{"key":"ref_96","doi-asserted-by":"crossref","first-page":"20417314251345000","DOI":"10.1177\/20417314251345000","article-title":"Lack of Biochemical Signalling in GelMA Leads to Polarity Reversion in Intestinal Organoids Independent from Mechanoreciprocity","volume":"16","author":"Vanhove","year":"2025","journal-title":"J. Tissue Eng."},{"key":"ref_97","doi-asserted-by":"crossref","first-page":"23240","DOI":"10.1038\/s41598-024-73774-1","article-title":"3D Bioprinting of Fish Skin-Based Gelatin Methacryloyl (GelMA) Bio-Ink for Use as a Potential Skin Substitute","volume":"14","author":"Tanadchangsaeng","year":"2024","journal-title":"Sci. Rep."},{"key":"ref_98","doi-asserted-by":"crossref","first-page":"70284","DOI":"10.1021\/acsami.4c16397","article-title":"Broadly Accessible 3D In Vitro Skin Model as a Comprehensive Platform for Antibacterial Therapy Screening","volume":"16","author":"Villata","year":"2024","journal-title":"ACS Appl. Mater. Interfaces"},{"key":"ref_99","doi-asserted-by":"crossref","first-page":"9009","DOI":"10.1016\/j.biomaterials.2012.08.068","article-title":"Directed Endothelial Cell Morphogenesis in Micropatterned Gelatin Methacrylate Hydrogels","volume":"33","author":"Nikkhah","year":"2012","journal-title":"Biomaterials"},{"key":"ref_100","doi-asserted-by":"crossref","first-page":"531","DOI":"10.4014\/jmb.2111.11003","article-title":"3D Bioprinted GelMA\/PEGDA Hybrid Scaffold for Establishing an in Vitro Model of Melanoma","volume":"32","author":"Duan","year":"2022","journal-title":"J. Microbiol. Biotechnol."},{"key":"ref_101","doi-asserted-by":"crossref","first-page":"17727","DOI":"10.1038\/s41598-021-97059-z","article-title":"Gelatin Methacrylate Hydrogels Culture Model for Glioblastoma Cells Enriches for Mesenchymal-like State and Models Interactions with Immune Cells","volume":"11","author":"Shah","year":"2021","journal-title":"Sci. Rep."},{"key":"ref_102","doi-asserted-by":"crossref","first-page":"e00310","DOI":"10.1016\/j.bprint.2023.e00310","article-title":"3D Bioprinted GelMA Platform for the Production of Lung Tumor Spheroids","volume":"36","author":"Villata","year":"2023","journal-title":"Bioprinting"},{"key":"ref_103","doi-asserted-by":"crossref","unstructured":"Kamiya, J., Kang, W., Yoshida, K., Takagi, R., Kanai, S., Hanai, M., Nakamura, A., Yamada, M., Miyamoto, Y., and Miyado, M. (2020). Suppression of Non-Random Fertilization by MHC Class I Antigens. Int. J. Mol. Sci., 21.","DOI":"10.3390\/ijms21228731"},{"key":"ref_104","doi-asserted-by":"crossref","first-page":"065017","DOI":"10.1088\/1748-605X\/aba40c","article-title":"Effect of Sterilization Treatment on Mechanical Properties, Biodegradation, Bioactivity and Printability of GelMA Hydrogels","volume":"15","author":"Rizwan","year":"2020","journal-title":"Biomed. Mater."},{"key":"ref_105","doi-asserted-by":"crossref","first-page":"015015","DOI":"10.1088\/1748-605X\/aca4b2","article-title":"Effects of Sterilization Methods on Gelatin Methacryloyl Hydrogel Properties and Macrophage Gene Expression in Vitro","volume":"18","author":"Zhang","year":"2023","journal-title":"Biomed. Mater."},{"key":"ref_106","doi-asserted-by":"crossref","first-page":"035023","DOI":"10.1088\/1758-5090\/abec2d","article-title":"Printability and Bio-Functionality of a Shear Thinning Methacrylated Xanthan\u2013Gelatin Composite Bioink","volume":"13","author":"Postma","year":"2021","journal-title":"Biofabrication"},{"key":"ref_107","doi-asserted-by":"crossref","first-page":"112370","DOI":"10.1016\/j.eurpolymj.2023.112370","article-title":"A Versatile GelMA Composite Hydrogel: Designing Principles, Delivery Forms and Biomedical Applications","volume":"197","author":"Zhang","year":"2023","journal-title":"Eur. Polym. 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