{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,2]],"date-time":"2026-04-02T16:05:22Z","timestamp":1775145922976,"version":"3.50.1"},"reference-count":50,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2022,2,20]],"date-time":"2022-02-20T00:00:00Z","timestamp":1645315200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Gels"],"abstract":"<jats:p>This study aims to investigate the effect of new natural photosensitizers (PS) (based on oregano essential oil, curcuma extract, and arnica oil) through in vitro cytotoxicity and biological tests in rat-induced periodontal disease, treated with photodynamic therapy (aPDT). The cytotoxicity of PS was performed on human dental pulp mesenchymal stem cells (dMSCs) and human keratinocyte (HaCaT) cell lines. Periodontal disease was induced by ligation of the first mandibular molar of 25 rats, which were divided into 5 groups: control group, periodontitis group, Curcuma and aPDT-treated group, oregano and aPDT-treated group, and aPDT group. The animals were euthanized after 4 weeks of study. Computed tomography imaging has been used to evaluate alveolar bone loss. Hematological and histological evaluation showed a greater magnitude of the inflammatory response and severe destruction of the periodontal ligaments in the untreated group.. For the group with the induced periodontitis and treated with natural photosensitizers, the aPDT improved the results; this therapy could be an important adjuvant treatment. The obtained results of these preliminary studies encourage us to continue the research of periodontitis treated with natural photosensitizers activated by photodynamic therapy.<\/jats:p>","DOI":"10.3390\/gels8020134","type":"journal-article","created":{"date-parts":[[2022,2,21]],"date-time":"2022-02-21T08:18:05Z","timestamp":1645431485000},"page":"134","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Photodynamic Therapy with Natural Photosensitizers in the Management of Periodontal Disease Induced in Rats"],"prefix":"10.3390","volume":"8","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8923-6639","authenticated-orcid":false,"given":"Laura Monica","family":"Dascalu (Rusu)","sequence":"first","affiliation":[{"name":"Department of Prosthodontics and Dental Materials, Iuliu Hatieganu University of Medicine and Pharmacy, 31 Avram Iancu Str., 400083 Cluj-Napoca, Romania"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9714-4809","authenticated-orcid":false,"given":"Marioara","family":"Moldovan","sequence":"additional","affiliation":[{"name":"Raluca Ripan Institute of Chemistry, Babes-Bolyai University, 30 Fantanele Str., 400294 Cluj-Napoca, Romania"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2960-9832","authenticated-orcid":false,"given":"Codruta","family":"Sarosi","sequence":"additional","affiliation":[{"name":"Raluca Ripan Institute of Chemistry, Babes-Bolyai University, 30 Fantanele Str., 400294 Cluj-Napoca, Romania"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sorina","family":"Sava","sequence":"additional","affiliation":[{"name":"Department of Prosthodontics and Dental Materials, Iuliu Hatieganu University of Medicine and Pharmacy, 31 Avram Iancu Str., 400083 Cluj-Napoca, Romania"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alexandra","family":"Dreanca","sequence":"additional","affiliation":[{"name":"Pathophysiology\/Toxicology Department, Faculty of Veterinary Medicine, University of Agricultural Science and Veterinary Medicine, 3-5 Calea Manastur, 400372 Cluj-Napoca, Romania"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Calin","family":"Repciuc","sequence":"additional","affiliation":[{"name":"Pathophysiology\/Toxicology Department, Faculty of Veterinary Medicine, University of Agricultural Science and Veterinary Medicine, 3-5 Calea Manastur, 400372 Cluj-Napoca, Romania"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6486-783X","authenticated-orcid":false,"given":"Robert","family":"Purdoiu","sequence":"additional","affiliation":[{"name":"Pathophysiology\/Toxicology Department, Faculty of Veterinary Medicine, University of Agricultural Science and Veterinary Medicine, 3-5 Calea Manastur, 400372 Cluj-Napoca, Romania"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andras","family":"Nagy","sequence":"additional","affiliation":[{"name":"Pathophysiology\/Toxicology Department, Faculty of Veterinary Medicine, University of Agricultural Science and Veterinary Medicine, 3-5 Calea Manastur, 400372 Cluj-Napoca, Romania"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M\u00eendra Eugenia","family":"Badea","sequence":"additional","affiliation":[{"name":"Department of Preventive Dental Medicine, Iuliu Hatieganu University of Medicine and Pharmacy, 31 Avram Iancu Str., 400083 Cluj-Napoca, Romania"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ariadna Georgiana","family":"Paun","sequence":"additional","affiliation":[{"name":"Department Community Medicine, Iuliu Ha\u021bieganu University of Medicine and Pharmacy, 31 Avram Iancu Str., 400083 Cluj-Napoca, Romania"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Iulia Clara","family":"Badea","sequence":"additional","affiliation":[{"name":"Department of Preventive Dental Medicine, Iuliu Hatieganu University of Medicine and Pharmacy, 31 Avram Iancu Str., 400083 Cluj-Napoca, Romania"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8262-2166","authenticated-orcid":false,"given":"Radu","family":"Chifor","sequence":"additional","affiliation":[{"name":"Department of Preventive Dental Medicine, Iuliu Hatieganu University of Medicine and Pharmacy, 31 Avram Iancu Str., 400083 Cluj-Napoca, Romania"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2022,2,20]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"932","DOI":"10.1128\/AAC.47.3.932-940.2003","article-title":"In Vivo Killing of Porphyromonas gingivalis by Toluidine Blue-Mediated Photosensitization in an Animal Model","volume":"47","author":"Nakanishi","year":"2003","journal-title":"Antimicrob. 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