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Achieving on-demand release of nano-drugs in specific infection environments is highly attractive. Herein, we constructed a \u201ccore-shell\u201d nanogel (G@CuS) based on a copper sulfide (CuS) antimicrobial agent and gelatin for targeted drug release and bacterial clearance in a gelatinase infected microenvironment. G@CuS produced heat and reactive oxygen species (ROS) under the irradiation of a laser, which together with the released Cu2+ cause irreversible and efficient physical damage to the bacteria. Moreover, the encapsulation of gelatin not only limits the biotoxicity of CuS nanodots (NDs), but also effectively promotes the proliferation of mammalian cells. Under the synergy of multiple mechanisms, G@CuS eradicated the colonized bacteria in the wound of mice infected with Staphylococcus aureus (S. aureus) and accelerated wound healing. The proposed application strategy of nanogel is expected to provide a new idea for clinical transformation.<\/jats:p>","DOI":"10.3390\/gels8070397","type":"journal-article","created":{"date-parts":[[2022,6,23]],"date-time":"2022-06-23T21:25:00Z","timestamp":1656019500000},"page":"397","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Gelatinase Responsive Nanogel for Antibacterial Phototherapy and Wound Healing"],"prefix":"10.3390","volume":"8","author":[{"given":"Qianqian","family":"Han","sequence":"first","affiliation":[{"name":"School of Pharmacy, Changzhou University, Changzhou 213164, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xuan","family":"Wang","sequence":"additional","affiliation":[{"name":"School of Pharmacy, Changzhou University, Changzhou 213164, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6514-6006","authenticated-orcid":false,"given":"Lin","family":"Qiu","sequence":"additional","affiliation":[{"name":"School of Pharmacy, Changzhou University, Changzhou 213164, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinpei","family":"Zhou","sequence":"additional","affiliation":[{"name":"School of Pharmacy, Changzhou University, Changzhou 213164, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zexuan","family":"Hui","sequence":"additional","affiliation":[{"name":"School of Pharmacy, Changzhou University, Changzhou 213164, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinye","family":"Ni","sequence":"additional","affiliation":[{"name":"The Affiliated Changzhou No. 2 People\u2019s Hospital of Nanjing Medical University, Changzhou 213003, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yang","family":"Xuan","sequence":"additional","affiliation":[{"name":"Key Lab of Biotechnology and Bioresources Utilization of Ministry of Education, College of Life Science, Dalian Minzu University, Dalian 116600, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoling","family":"Lei","sequence":"additional","affiliation":[{"name":"College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianhao","family":"Wang","sequence":"additional","affiliation":[{"name":"School of Pharmacy, Changzhou University, Changzhou 213164, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,6,23]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"165","DOI":"10.1111\/srt.12424","article-title":"The relationship between skin function, barrier properties, and body-dependent factors","volume":"24","author":"Spano","year":"2018","journal-title":"Skin Res. 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