{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,4]],"date-time":"2026-04-04T12:46:21Z","timestamp":1775306781095,"version":"3.50.1"},"reference-count":54,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2019,1,17]],"date-time":"2019-01-17T00:00:00Z","timestamp":1547683200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003329","name":"Ministerio de Econom\u00eda y Competitividad","doi-asserted-by":"publisher","award":["CB06\/07\/0023 SAF2015-64152-R"],"award-info":[{"award-number":["CB06\/07\/0023 SAF2015-64152-R"]}],"id":[{"id":"10.13039\/501100003329","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000780","name":"European Commission","doi-asserted-by":"publisher","award":["HEALTH-F5-2012-305421 P-SPHERE COFUND"],"award-info":[{"award-number":["HEALTH-F5-2012-305421 P-SPHERE COFUND"]}],"id":[{"id":"10.13039\/501100000780","id-type":"DOI","asserted-by":"publisher"}]},{"name":"FPU","award":["FPU2013\/00754"],"award-info":[{"award-number":["FPU2013\/00754"]}]},{"name":"Juan de la Cierva","award":["JCI-2011-10660"],"award-info":[{"award-number":["JCI-2011-10660"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Genes"],"abstract":"<jats:p>XPF endonuclease is one of the most important DNA repair proteins. Encoded by XPF\/ERCC4, XPF provides the enzymatic activity of XPF-ERCC1 heterodimer, an endonuclease that incises at the 5\u2019 side of various DNA lesions. XPF is essential for nucleotide excision repair (NER) and interstrand crosslink repair (ICLR). XPF\/ERCC4 mutations are associated with several human diseases: Xeroderma Pigmentosum (XP), Segmental Progeria (XFE), Fanconi Anemia (FA), Cockayne Syndrome (CS), and XP\/CS combined disease (XPCSCD). Most affected individuals are compound heterozygotes for XPF\/ERCC4 mutations complicating the identification of genotype\/phenotype correlations. We report a detailed overview of NER and ICLR functional studies in human XPF-KO (knock-out) isogenic cells expressing six disease-specific pathogenic XPF amino acid substitution mutations. Ultraviolet (UV) sensitivity and unscheduled DNA synthesis (UDS) assays provide the most reliable information to discern mutations associated with ICLR impairment from mutations related to NER deficiency, whereas recovery of RNA synthesis (RRS) assays results hint to a possible role of XPF in resolving R-loops. Our functional studies demonstrate that a defined cellular phenotype cannot be easily correlated to each XPF mutation. Substituted positions along XPF sequences are not predictive of cellular phenotype nor reflect a particular disease. Therefore, in addition to mutation type, allelic interactions, protein stability and intracellular distribution of mutant proteins may also contribute to alter DNA repair pathways balance leading to clinically distinct disorders.<\/jats:p>","DOI":"10.3390\/genes10010060","type":"journal-article","created":{"date-parts":[[2019,1,17]],"date-time":"2019-01-17T11:30:27Z","timestamp":1547724627000},"page":"60","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Functional Comparison of XPF Missense Mutations Associated to Multiple DNA Repair Disorders"],"prefix":"10.3390","volume":"10","author":[{"given":"Maria","family":"Mar\u00edn","sequence":"first","affiliation":[{"name":"Departament de Gen\u00e8tica i de Microbiologia, Universitat Aut\u00f2noma de Barcelona, 08028 Barcelona, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mar\u00eda Jos\u00e9","family":"Ram\u00edrez","sequence":"additional","affiliation":[{"name":"Departament de Gen\u00e8tica i de Microbiologia, Universitat Aut\u00f2noma de Barcelona, 08028 Barcelona, Spain"},{"name":"Centro de Investigaci\u00f3n Biom\u00e9dica en Red de Enfermedades Raras (CIBERER), 08028 Barcelona, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Miriam","family":"Aza Carmona","sequence":"additional","affiliation":[{"name":"Departament de Gen\u00e8tica i de Microbiologia, Universitat Aut\u00f2noma de Barcelona, 08028 Barcelona, Spain"},{"name":"Institute of Medical and Molecular Genetics (INGEMM), Hospital Universitario La Paz, 28029 Madrid, Spain"},{"name":"CIBERER, ISCIII, 28029 Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nan","family":"Jia","sequence":"additional","affiliation":[{"name":"Department of Genetics, Research Institute of Environmental Medicine (RIeM), Nagoya University, Nagoya, Japan\/Department of Human Genetics and Molecular Biology, Graduate School of Medicine, Nagoya University, Nagoya 464-0805, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tomoo","family":"Ogi","sequence":"additional","affiliation":[{"name":"Department of Genetics, Research Institute of Environmental Medicine (RIeM), Nagoya University, Nagoya, Japan\/Department of Human Genetics and Molecular Biology, Graduate School of Medicine, Nagoya University, Nagoya 464-0805, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Massimo","family":"Bogliolo","sequence":"additional","affiliation":[{"name":"Departament de Gen\u00e8tica i de Microbiologia, Universitat Aut\u00f2noma de Barcelona, 08028 Barcelona, Spain"},{"name":"Centro de Investigaci\u00f3n Biom\u00e9dica en Red de Enfermedades Raras (CIBERER), 08028 Barcelona, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4041-7519","authenticated-orcid":false,"given":"Jordi","family":"Surrall\u00e9s","sequence":"additional","affiliation":[{"name":"Departament de Gen\u00e8tica i de Microbiologia, Universitat Aut\u00f2noma de Barcelona, 08028 Barcelona, Spain"},{"name":"Centro de Investigaci\u00f3n Biom\u00e9dica en Red de Enfermedades Raras (CIBERER), 08028 Barcelona, Spain"},{"name":"Genetics Department Institute of Biomedical Research, Hospital de la Santa Creu i Sant Pau, 08025 Barcelona, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2019,1,17]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"6553","DOI":"10.1128\/MCB.16.11.6553","article-title":"ERCC4 (XPF) encodes a human nucleotide excision repair protein with eukaryotic recombination homologs","volume":"16","author":"Brookman","year":"1996","journal-title":"Mol. 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