{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T21:05:18Z","timestamp":1790715918227,"version":"4.1.0"},"reference-count":66,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2019,10,31]],"date-time":"2019-10-31T00:00:00Z","timestamp":1572480000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Russian Science Foundation grant","award":["19-74-20130 (E.S.)"],"award-info":[{"award-number":["19-74-20130 (E.S.)"]}]},{"name":"Croatian Science Foundation grant","award":["IP-2016-06-8861 (I.I.B.)"],"award-info":[{"award-number":["IP-2016-06-8861 (I.I.B.)"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Genes"],"abstract":"<jats:p>Bacteria and archaea use CRISPR-Cas adaptive immunity systems to interfere with viruses, plasmids, and other mobile genetic elements. During the process of adaptation, CRISPR-Cas systems acquire immunity by incorporating short fragments of invaders\u2019 genomes into CRISPR arrays. The acquisition of fragments of host genomes leads to autoimmunity and may drive chromosomal rearrangements, negative cell selection, and influence bacterial evolution. In this study, we investigated the role of proteins involved in genome stability maintenance in spacer acquisition by the Escherichia coli type I-E CRISPR-Cas system targeting its own genome. We show here, that the deletion of recJ decreases adaptation efficiency and affects accuracy of spacers incorporation into CRISPR array. Primed adaptation efficiency is also dramatically inhibited in double mutants lacking recB and sbcD but not in single mutants suggesting independent involvement and redundancy of RecBCD and SbcCD pathways in spacer acquisition. While the presence of at least one of two complexes is crucial for efficient primed adaptation, RecBCD and SbcCD affect the pattern of acquired spacers. Overall, our data suggest distinct roles of the RecBCD and SbcCD complexes and of RecJ in spacer precursor selection and insertion into CRISPR array and highlight the functional interplay between CRISPR-Cas systems and host genome maintenance mechanisms.<\/jats:p>","DOI":"10.3390\/genes10110872","type":"journal-article","created":{"date-parts":[[2019,10,31]],"date-time":"2019-10-31T06:33:29Z","timestamp":1572503609000},"page":"872","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Genome Maintenance Proteins Modulate Autoimmunity Mediated Primed Adaptation by the Escherichia coli Type I-E CRISPR-Cas System"],"prefix":"10.3390","volume":"10","author":[{"given":"Elena","family":"Kurilovich","sequence":"first","affiliation":[{"name":"Center of Life Sciences, Skolkovo Institute of Science and Technology, Moscow 143028, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4271-1658","authenticated-orcid":false,"given":"Anna","family":"Shiriaeva","sequence":"additional","affiliation":[{"name":"Center of Life Sciences, Skolkovo Institute of Science and Technology, Moscow 143028, Russia"},{"name":"Waksman Institute, Rutgers, the State University of New Jersey, Piscataway, NJ 08854, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anastasia","family":"Metlitskaya","sequence":"additional","affiliation":[{"name":"Institute of Molecular Genetics, Russian Academy of Sciences, Moscow 123182, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Natalia","family":"Morozova","sequence":"additional","affiliation":[{"name":"Center of Life Sciences, Skolkovo Institute of Science and Technology, Moscow 143028, Russia"},{"name":"Peter the Great St Petersburg State Polytechnic University, St Petersburg 195251, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ivana","family":"Ivancic-Bace","sequence":"additional","affiliation":[{"name":"Department of Biology, Faculty of Science, University of Zagreb, Horvatovac 102a, 10000 Zagreb, Croatia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Konstantin","family":"Severinov","sequence":"additional","affiliation":[{"name":"Center of Life Sciences, Skolkovo Institute of Science and Technology, Moscow 143028, Russia"},{"name":"Waksman Institute, Rutgers, the State University of New Jersey, Piscataway, NJ 08854, USA"},{"name":"Center for Precision Genome Editing and Genetic Technologies for Biomedicine, Institute of Gene Biology, Russian Academy of Sciences, 34\/5 Vavilov St., Moscow 119334, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ekaterina","family":"Savitskaya","sequence":"additional","affiliation":[{"name":"Center of Life Sciences, Skolkovo Institute of Science and Technology, Moscow 143028, Russia"},{"name":"Institute of Molecular Genetics, Russian Academy of Sciences, Moscow 123182, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2019,10,31]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1709","DOI":"10.1126\/science.1138140","article-title":"CRISPRProvides Acquired Resistance Against Viruses in Prokaryotes","volume":"315","author":"Barrangou","year":"2007","journal-title":"Science"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"960","DOI":"10.1126\/science.1159689","article-title":"Small CRISPR RNAs Guide Antiviral Defense in Prokaryotes","volume":"321","author":"Brouns","year":"2008","journal-title":"Science"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"1843","DOI":"10.1126\/science.1165771","article-title":"CRISPR Interference Limits Horizontal Gene Transfer in Staphylococci by Targeting DNA","volume":"322","author":"Marraffini","year":"2008","journal-title":"Science"},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Ca\u00f1ez, C., Selle, K., Goh, Y.J., and Barrangou, R. 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