{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,2]],"date-time":"2026-05-02T18:30:27Z","timestamp":1777746627381,"version":"3.51.4"},"reference-count":60,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2020,2,15]],"date-time":"2020-02-15T00:00:00Z","timestamp":1581724800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Key R &amp; D program of China","award":["2016YFD0102000"],"award-info":[{"award-number":["2016YFD0102000"]}]},{"name":"Jilin Province Science and Technology Department Science and Technology Program of China","award":["20190301074NY"],"award-info":[{"award-number":["20190301074NY"]}]},{"name":"Jilin Province Education Department &quot;13th Five-Year&quot; Science and Technology program of China","award":["JJKH20180729KJ"],"award-info":[{"award-number":["JJKH20180729KJ"]}]},{"DOI":"10.13039\/501100001809","name":"the National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["NSFC31501235"],"award-info":[{"award-number":["NSFC31501235"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"he National Key R &amp; D program of China","award":["2018YFD0300207"],"award-info":[{"award-number":["2018YFD0300207"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Genes"],"abstract":"<jats:p>Interspecific hybridization creates genetic variation useful for crop improvement. However, whether pollen from a different genus affects the genomic stability and\/or transcriptome of the recipient species during intergeneric pollination has not been investigated. Here, we crossed japonica rice cv. Z12 with the maize accession B73 (pollen donor) and obtained a morphologically stable line, MU1, exhibiting moderate dwarfism, higher tiller number, and increased grain weight compared with Z12. To reveal the genetic basis of these morphological changes in MU1, we performed whole-genome resequencing of MU1 and Z12. Compared with Z12, MU1 showed 107,250 single nucleotide polymorphisms (SNPs) and 23,278 insertion\/deletions (InDels). Additionally, 5\u2019-upstream regulatory regions (5\u2019UTRs) of 429 and 309 differentially expressed genes (DEGs) in MU1 contained SNPs and InDels, respectively, suggesting that a subset of these DEGs account for the variation in 5\u2019UTRs. Transcriptome analysis revealed 2190 DEGs in MU1 compared with Z12. Genes up-regulated in MU1 were mainly involved in photosynthesis, generation of precursor metabolites, and energy and cellular biosynthetic processes; whereas those down-regulated in MU1 were involved in plant hormone signal transduction pathway and response to stimuli and stress processes. Quantitative PCR (qPCR) further identified the expression levels of the up- or down-regulated gene in plant hormone signal transduction pathway. The expression level changes of plant hormone signal transduction pathway may be significant for plant growth and development. These findings suggest that mutations caused by intergeneric pollination could be the important reason for changes of MU1 in agronomic traits.<\/jats:p>","DOI":"10.3390\/genes11020199","type":"journal-article","created":{"date-parts":[[2020,2,25]],"date-time":"2020-02-25T08:12:22Z","timestamp":1582618342000},"page":"199","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Examination of Genomic and Transcriptomic Alterations in a Morphologically Stable Line, MU1, Generated by Intergeneric Pollination"],"prefix":"10.3390","volume":"11","author":[{"given":"Wei-Long","family":"Meng","sequence":"first","affiliation":[{"name":"College of Agronomy, Jilin Agricultural University, Changchun 130118, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Meng-Jie","family":"Zhao","sequence":"additional","affiliation":[{"name":"Key Laboratory of Biology and Genetic Improvement of Triticeae Crops, Ministry of Agriculture, Institute of Crop Science, Chinese Academy of Agricultural Sciences (CAAS)\/National Key Facility for Crop Gene Resources and Genetic Improvement, Beijing 100081, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiang-Bo","family":"Yang","sequence":"additional","affiliation":[{"name":"College of Agronomy, Jilin Agricultural Science and Technology University, Jilin 132101, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"An-Xing","family":"Zhang","sequence":"additional","affiliation":[{"name":"College of Agronomy, Jilin Agricultural University, Changchun 130118, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ning-Ning","family":"Wang","sequence":"additional","affiliation":[{"name":"College of Agronomy, Jilin Agricultural University, Changchun 130118, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8028-6413","authenticated-orcid":false,"given":"Zhao-Shi","family":"Xu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Biology and Genetic Improvement of Triticeae Crops, Ministry of Agriculture, Institute of Crop Science, Chinese Academy of Agricultural Sciences (CAAS)\/National Key Facility for Crop Gene Resources and Genetic Improvement, Beijing 100081, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jian","family":"Ma","sequence":"additional","affiliation":[{"name":"College of Agronomy, Jilin Agricultural University, Changchun 130118, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2020,2,15]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Wang, H., Chai, Y., Chu, X.C., Zhao, Y.Y., Wu, Y., Zhao, J.H., Ngezahayo, F., Xu, C.M., and Liu, B. (2009). Molecular characterization of a rice mutator-phenotype derived from an incompatible cross-pollination reveals transgenerational mobilization of multiple transposable elements and extensive epigenetic instability. BMC Plant Biol., 9.","DOI":"10.1186\/1471-2229-9-63"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"221","DOI":"10.1111\/j.1469-8137.2006.01875.x","article-title":"Production of reactive oxygen species and reactive nitrogen species by angiosperm stigmas and pollen: Potential signalling crosstalk?","volume":"172","author":"McInnis","year":"2006","journal-title":"New Phytol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"768","DOI":"10.1007\/BF00266656","article-title":"Inheritance of plastids in interspecific hybrids of blue spruce and white spruce","volume":"78","author":"Stine","year":"1989","journal-title":"Theor. Appl. Genet."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"196","DOI":"10.1007\/s00122-006-0286-2","article-title":"Extent and pattern of DNA methylation alteration in rice lines derived from introgressive hybridization of rice and Zizania latifolia Griseb","volume":"113","author":"Dong","year":"2006","journal-title":"Theor. Appl. Genet."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"931","DOI":"10.1093\/oxfordjournals.molbev.a026182","article-title":"Interspecific hybridization increases transposition rates of Osvaldo","volume":"16","author":"Labrador","year":"1999","journal-title":"Mol. Biol. Evol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"571","DOI":"10.1023\/B:PLAN.0000038270.48326.7a","article-title":"Extensive alterations in DNA methylation and transcription in rice caused by introgression from Zizania latifolia","volume":"54","author":"Liu","year":"2004","journal-title":"Plant Mol. Biol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"1945","DOI":"10.1534\/genetics.105.040964","article-title":"Extensive de Novo genomic variation in rice induced by introgression from wild rice (Zizania latifolia Griseb.)","volume":"170","author":"Wang","year":"2005","journal-title":"Genetics"},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Wang, Z.H., Zhang, D., Bai, Y., Zhang, Y.H., Liu, Y., Wu, Y., Lin, X.Y., Wen, J.W., Xu, C.M., and Li, L.F. (2013). Genomewide variation in an introgression line of rice-Zizania revealed by whole-genome re-sequencing. PLoS ONE, 8.","DOI":"10.1371\/journal.pone.0074479"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"546","DOI":"10.3732\/ajb.1200359","article-title":"Variation in the strength of association among pollination systems and floral traits: Evolutionary changes in the floral traits of Bornean gingers (Zingiberaceae)","volume":"100","author":"Sakai","year":"2013","journal-title":"Am. J. Bot."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"307","DOI":"10.1016\/j.tree.2013.01.019","article-title":"Pollinator-mediated evolution of floral signals","volume":"28","author":"Schiestl","year":"2013","journal-title":"Trends Ecol. Evol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"1014","DOI":"10.1093\/mp\/ssr022","article-title":"Genetic and epigenetic effects of plant-pathogen interactions: An evolutionary perspective","volume":"4","author":"Boyko","year":"2011","journal-title":"Mol. Plant"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"E2183","DOI":"10.1073\/pnas.1209329109","article-title":"Widespread dynamic DNA methylation in response to biotic stress","volume":"109","author":"Dowen","year":"2012","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"618","DOI":"10.1016\/j.tree.2012.07.011","article-title":"Transgenerational defense induction and epigenetic inheritance in plants","volume":"27","author":"Holeski","year":"2012","journal-title":"Trends Ecol. Evol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"38","DOI":"10.1111\/mec.14390","article-title":"Does introgression of crop alleles into wild and weedy living populations create cryptic in situ germplasm banks?","volume":"27","author":"Ellstrand","year":"2018","journal-title":"Mol. Ecol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"658","DOI":"10.1002\/ps.3850","article-title":"Dealing with transgene flow of crop protection traits from crops to their relatives","volume":"71","author":"Gressel","year":"2015","journal-title":"Pest. Manag. Sci."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"2103","DOI":"10.1111\/j.1365-294X.2008.03715.x","article-title":"Speciation with gene flow could be common","volume":"17","author":"Nosil","year":"2008","journal-title":"Mol. Ecol."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"910","DOI":"10.1126\/science.1137729","article-title":"Plant speciation","volume":"317","author":"Rieseberg","year":"2007","journal-title":"Science"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"377","DOI":"10.1146\/annurev.arplant.58.032806.103835","article-title":"Genetic and epigenetic mechanisms for gene expression and phenotypic variation in plant polyploids","volume":"58","author":"Chen","year":"2007","journal-title":"Annu. Rev. Plant Biol."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"3403","DOI":"10.1105\/tpc.107.054346","article-title":"Genomic changes in resynthesized Brassica napus and their effect on gene expression and phenotype","volume":"19","author":"Gaeta","year":"2007","journal-title":"Plant Cell"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"733","DOI":"10.1104\/pp.003095","article-title":"Remodeling of DNA methylation and phenotypic and transcriptional changes in synthetic Arabidopsis allotetraploids","volume":"129","author":"Madlung","year":"2002","journal-title":"Plant Physiol."},{"key":"ref_21","first-page":"1735","article-title":"Allopolyploidy-induced rapid genome evolution in the wheat (Aegilops-Triticum) group","volume":"13","author":"Ozkan","year":"2001","journal-title":"Plant Cell"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"1163","DOI":"10.1111\/j.1365-294X.2005.02488.x","article-title":"Genetic and epigenetic consequences of recent hybridization and polyploidy in Spartina (Poaceae)","volume":"14","author":"Salmon","year":"2005","journal-title":"Mol. Ecol."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"1749","DOI":"10.1105\/TPC.010083","article-title":"Sequence elimination and cytosine methylation are rapid and reproducible responses of the genome to wide hybridization and allopolyploidy in wheat","volume":"13","author":"Shaked","year":"2001","journal-title":"Plant Cell"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"212","DOI":"10.1111\/j.1365-313X.2011.04679.x","article-title":"De novo genetic variation associated with retrotransposon activation, genomic rearrangements and trait variation in a recombinant inbred line population of Brassica napus derived from interspecific hybridization with Brassica rapa","volume":"68","author":"Zou","year":"2011","journal-title":"Plant J."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"792","DOI":"10.1126\/science.15739260","article-title":"The significance of responses of the genome to challenge","volume":"226","author":"McClintock","year":"1984","journal-title":"Science"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"2261","DOI":"10.1101\/gr.2924904","article-title":"An active murine transposon family pair: Retrotransposition of \"master\" MusD copies and ETn trans-mobilization","volume":"14","author":"Ribet","year":"2004","journal-title":"Genome Res."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"1099","DOI":"10.1007\/s001220051173","article-title":"Production of a highly asymmetric somatic hybrid between rice and Zizania latifolia (Griseb): Evidence for inter-genomic exchange","volume":"98","author":"Liu","year":"1999","journal-title":"Theor. Appl. Genet."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"19529","DOI":"10.1073\/pnas.1217149109","article-title":"Strong signatures of selection in the domestic pig genome","volume":"109","author":"Rubin","year":"2012","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"1211","DOI":"10.1126\/science.1086949","article-title":"Major ecological transitions in wild sunflowers facilitated by hybridization","volume":"301","author":"Rieseberg","year":"2003","journal-title":"Science"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"W122","DOI":"10.1093\/nar\/gkx382","article-title":"agriGO v2.0: A GO analysis toolkit for the agricultural community, 2017 update","volume":"45","author":"Tian","year":"2017","journal-title":"Nucleic Acids Res."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"R36","DOI":"10.1186\/gb-2013-14-4-r36","article-title":"TopHat2: Accurate alignment of transcriptomes in the presence of insertions, deletions and gene fusions","volume":"14","author":"Kim","year":"2013","journal-title":"Genome Biol."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"46","DOI":"10.1038\/nbt.2450","article-title":"Differential analysis of gene regulation at transcript resolution with RNA-seq","volume":"31","author":"Trapnell","year":"2013","journal-title":"Nat. Biotechnol."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"325","DOI":"10.1007\/BF02772676","article-title":"Comparison of reference genes for quantitative real-time polymerase chain reaction analysis of gene expression in sugarcane","volume":"22","author":"Iskandar","year":"2004","journal-title":"Plant Mol. Biol. Rep."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1093\/nar\/28.1.27","article-title":"KEGG: Kyoto Encyclopedia of Genes and Genomes","volume":"28","author":"Minoru","year":"2000","journal-title":"Nucleic Acids Res."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"R365","DOI":"10.1016\/j.cub.2011.03.013","article-title":"Hormone signalling crosstalk in plant growth regulation","volume":"21","author":"Depuydt","year":"2011","journal-title":"Curr. Biol."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"618","DOI":"10.1016\/j.bbadis.2013.01.021","article-title":"Pro-inflammatory cytokines modulate iron regulatory protein 1 expression and iron transportation through reactive oxygen\/nitrogen species production in ventral mesencephalic neurons","volume":"1832","author":"Wang","year":"2013","journal-title":"Biochim. Biophys. Acta"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"497","DOI":"10.1038\/nature11532","article-title":"A map of rice genome variation reveals the origin of cultivated rice","volume":"490","author":"Huang","year":"2012","journal-title":"Nature"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"819","DOI":"10.1007\/s00299-004-0896-6","article-title":"Isolation and characterization of a Ds-tagged rice (Oryza sativa L.) GA-responsive dwarf mutant defective in an early step of the gibberellin biosynthesis pathway","volume":"23","author":"Zhou","year":"2005","journal-title":"Plant Cell Rep."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"687","DOI":"10.1007\/s11103-004-1692-y","article-title":"A role of OsGA20ox1, encoding an isoform of gibberellin 20-oxidase, for regulation of plant stature in rice","volume":"55","author":"Oikawa","year":"2004","journal-title":"Plant Mol. Biol."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"766","DOI":"10.1016\/j.bbrc.2015.03.104","article-title":"The rice ent-KAURENE SYNTHASE LIKE 2 encodes a functional ent-beyerene synthase","volume":"460","author":"Tezuka","year":"2015","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"1551","DOI":"10.1105\/tpc.12.9.1551","article-title":"Phenotypic instability and rapid gene silencing in newly formed arabidopsis allotetraploids","volume":"12","author":"Comai","year":"2000","journal-title":"Plant Cell"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1093\/aob\/mcp099","article-title":"Different genome-specific chromosome stabilities in synthetic Brassica allohexaploids revealed by wide crosses with Orychophragmus","volume":"104","author":"Ge","year":"2009","journal-title":"Ann. Bot."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1111\/j.1365-294X.2010.04926.x","article-title":"Nonadditive changes to cytosine methylation as a consequence of hybridization and genome duplication in Senecio (Asteraceae)","volume":"20","author":"Hegarty","year":"2011","journal-title":"Mol. Ecol."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"535","DOI":"10.1139\/g98-052","article-title":"Rapid genomic changes in newly synthesized amphiploids of Triticum and Aegilops. II. Changes in low-copy coding DNA sequences","volume":"41","author":"Liu","year":"1998","journal-title":"Genome"},{"key":"ref_45","doi-asserted-by":"crossref","unstructured":"Malinska, H., Tate, J.A., Matyasek, R., Leitch, A.R., Soltis, D.E., Soltis, P.S., and Kovarik, A. (2010). Similar patterns of rDNA evolution in synthetic and recently formed natural populations of Tragopogon (Asteraceae) allotetraploids. BMC Evol. Biol., 10.","DOI":"10.1186\/1471-2148-10-291"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1111\/j.1469-8137.2009.03140.x","article-title":"Mobilization of retrotransposons in synthetic allotetraploid tobacco","volume":"186","author":"Petit","year":"2010","journal-title":"New Phytol."},{"key":"ref_47","doi-asserted-by":"crossref","unstructured":"Cantu, D., Vanzetti, L.S., Sumner, A., Dubcovsky, M., Matvienko, M., Distelfeld, A., Michelmore, R.W., and Dubcovsky, J. (2010). Small RNAs, DNA methylation and transposable elements in wheat. BMC Genom., 11.","DOI":"10.1186\/1471-2164-11-408"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1007\/s00438-009-0504-0","article-title":"Genome-wide discovery of DNA polymorphism in Brassica rapa","volume":"283","author":"Park","year":"2010","journal-title":"Mol. Genet. Genom."},{"key":"ref_49","doi-asserted-by":"crossref","unstructured":"Wang, X.W., Zhao, Q.Y., Luan, J.B., Wang, Y.J., Yan, G.H., and Liu, S.S. (2012). Analysis of a native whitefly transcriptome and its sequence divergence with two invasive whitefly species. BMC Genom., 13.","DOI":"10.1186\/1471-2164-13-529"},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1038\/nbt.2050","article-title":"Resequencing 50 accessions of cultivated and wild rice yields markers for identifying agronomically important genes","volume":"30","author":"Xu","year":"2011","journal-title":"Nat. Biotechnol."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"1523","DOI":"10.1093\/molbev\/msp063","article-title":"Variation in the ratio of nucleotide substitution and indel rates across genomes in mammals and bacteria","volume":"26","author":"Chen","year":"2009","journal-title":"Mol. Biol. Evol."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"647","DOI":"10.1534\/genetics.111.128942","article-title":"Genetic variation and the fate of beneficial mutations in asexual populations","volume":"188","author":"Lang","year":"2011","journal-title":"Genetics"},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"1153","DOI":"10.1098\/rstb.2009.0317","article-title":"The population genetics of mutations: Good, bad and indifferent","volume":"365","author":"Loewe","year":"2010","journal-title":"Philos. Trans. R Soc. Lond. B Biol. Sci."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"1805","DOI":"10.1101\/gr.631202","article-title":"Interrogating a high-density SNP map for signatures of natural selection","volume":"12","author":"Akey","year":"2002","journal-title":"Genome. Res."},{"key":"ref_55","first-page":"1","article-title":"Photosynthesis, productivity and growth","volume":"10","author":"Harris","year":"1978","journal-title":"Adv. Limnol."},{"key":"ref_56","first-page":"1019","article-title":"The relationship between nitrogen, auxin and cytokinin in the growth regulation of rice (Oryza sativa L.) tiller buds","volume":"5","author":"Liu","year":"2011","journal-title":"Aust. J. Crop Sci."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"2603","DOI":"10.1105\/tpc.108.060913","article-title":"A Novel Class of Gibberellin 2-Oxidases Control Semidwarfism, Tillering, and Root Development in Rice","volume":"20","author":"Lo","year":"2008","journal-title":"Plant Cell"},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"1642","DOI":"10.1104\/pp.103.033696","article-title":"An overview of gibberellin metabolism enzyme genes and their related mutants in rice","volume":"134","author":"Sakamoto","year":"2004","journal-title":"Plant Physiol."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"741","DOI":"10.1126\/science.1113373","article-title":"Cytokinin oxidase regulates rice grain production","volume":"309","author":"Ashikari","year":"2005","journal-title":"Science"},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"10556","DOI":"10.1073\/pnas.91.22.10556","article-title":"Expression of the gene for a small GTP binding protein in transgenic tobacco elevates endogenous cytokinin levels, abnormally induces salicylic acid in response to wounding, and increases resistance to tobacco mosaic virus infection","volume":"91","author":"Sano","year":"1994","journal-title":"Proc. Natl. Acad. Sci. USA"}],"container-title":["Genes"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2073-4425\/11\/2\/199\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T08:58:05Z","timestamp":1760173085000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2073-4425\/11\/2\/199"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,2,15]]},"references-count":60,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2020,2]]}},"alternative-id":["genes11020199"],"URL":"https:\/\/doi.org\/10.3390\/genes11020199","relation":{},"ISSN":["2073-4425"],"issn-type":[{"value":"2073-4425","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,2,15]]}}}