{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T22:39:08Z","timestamp":1788993548110,"version":"build-2803163510"},"reference-count":53,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2020,5,6]],"date-time":"2020-05-06T00:00:00Z","timestamp":1588723200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Genes"],"abstract":"<jats:p>Stress-induced immunosuppression is a common problem in the poultry industry, but the specific mechanism of its effect on the immune function of chicken has not been clarified. In this study, 7-day-old Gushi cocks were selected as subjects, and a stress-induced immunosuppression model was successfully established via daily injection of 2.0 mg\/kg (body weight) dexamethasone. We characterized the spleen transcriptome in the control (B_S) and model (D_S) groups, and 515 significant differentially expressed genes (SDEGs) (Fragments Per Kilobase of transcript sequence per Millions base pairs sequenced (FPKM) &gt; 1, adjusted p-value (padj) &lt; 0.05 and Fold change (|FC|) \u2265 2) were identified. The cytokine-cytokine receptor interaction signaling pathway was identified as being highly activated during stress-induced immunosuppression, including the following SDEGs\u2014CXCL13L2, CSF3R, CSF2RB, CCR9, CCR10, IL1R1, IL8L1, IL8L2, GHR, KIT, OSMR, TNFRSF13B, TNFSF13B, and TGFBR2L. At the same time, immune-related SDEGs including CCR9, CCR10, DMB1, TNFRSF13B, TNFRSF13C and TNFSF13B were significantly enriched in the intestinal immune network for the IgA production signaling pathway. The SDEG protein-protein interaction module analysis showed that CXCR5, CCR8L, CCR9, CCR10, IL8L2, IL8L1, TNFSF13B, TNFRSF13B and TNFRSF13C may play an important role in stress-induced immunosuppression. These findings provide a background for further research on stress-induced immunosuppression. Thus, we can better understand the molecular genetic mechanism of chicken stress-induced immunosuppression.<\/jats:p>","DOI":"10.3390\/genes11050513","type":"journal-article","created":{"date-parts":[[2020,5,7]],"date-time":"2020-05-07T04:46:07Z","timestamp":1588826767000},"page":"513","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":22,"title":["Transcriptomic Analysis of Spleen Revealed Mechanism of Dexamethasone-Induced Immune Suppression in Chicks"],"prefix":"10.3390","volume":"11","author":[{"given":"Yujie","family":"Guo","sequence":"first","affiliation":[{"name":"College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou 450046, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aru","family":"Su","sequence":"additional","affiliation":[{"name":"College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou 450046, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huihui","family":"Tian","sequence":"additional","affiliation":[{"name":"College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou 450046, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Minxi","family":"Zhai","sequence":"additional","affiliation":[{"name":"College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou 450046, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenting","family":"Li","sequence":"additional","affiliation":[{"name":"College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou 450046, China"},{"name":"Henan Innovative Engineering Research Center of Poultry Germplasm Resource, Zhengzhou 450046, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yadong","family":"Tian","sequence":"additional","affiliation":[{"name":"College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou 450046, China"},{"name":"Henan Innovative Engineering Research Center of Poultry Germplasm Resource, Zhengzhou 450046, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kui","family":"Li","sequence":"additional","affiliation":[{"name":"College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou 450046, China"},{"name":"Henan Innovative Engineering Research Center of Poultry Germplasm Resource, Zhengzhou 450046, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2141-913X","authenticated-orcid":false,"given":"Guirong","family":"Sun","sequence":"additional","affiliation":[{"name":"College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou 450046, China"},{"name":"Henan Innovative Engineering Research Center of Poultry Germplasm Resource, Zhengzhou 450046, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruirui","family":"Jiang","sequence":"additional","affiliation":[{"name":"College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou 450046, China"},{"name":"Henan Innovative Engineering Research Center of Poultry Germplasm Resource, Zhengzhou 450046, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruili","family":"Han","sequence":"additional","affiliation":[{"name":"College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou 450046, China"},{"name":"Henan Innovative Engineering Research Center of Poultry Germplasm Resource, Zhengzhou 450046, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fengbin","family":"Yan","sequence":"additional","affiliation":[{"name":"College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou 450046, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9628-7135","authenticated-orcid":false,"given":"Xiangtao","family":"Kang","sequence":"additional","affiliation":[{"name":"Henan Innovative Engineering Research Center of Poultry Germplasm Resource, Zhengzhou 450046, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2020,5,6]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"841","DOI":"10.3382\/ps.2009-00483","article-title":"Understanding stress-induced immunosuppression: Exploration of cytokine and chemokine gene profiles in chicken peripheral leukocytes","volume":"89","author":"Shini","year":"2010","journal-title":"Poult. 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