{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,23]],"date-time":"2026-07-23T14:31:51Z","timestamp":1784817111120,"version":"3.55.0"},"reference-count":70,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2020,6,22]],"date-time":"2020-06-22T00:00:00Z","timestamp":1592784000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"ProteoRed, PRB3-ISCIII","award":["PT17\/0019"],"award-info":[{"award-number":["PT17\/0019"]}]},{"name":"Spanish National System of Science and Technology","award":["Spanish National project AGL2015-68248-C2-1-R"],"award-info":[{"award-number":["Spanish National project AGL2015-68248-C2-1-R"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Genes"],"abstract":"<jats:p>In plant and animal nematode parasites, proteins derived from esophageal gland cells have been shown to be important in the host-nematodes relationship but little is known about the allergenic potential of these proteins in the genus Anisakis. Taking into account the increase of anisakiasis and allergies related to these nematodes, immunoreactive properties of gland cell proteins were investigated. Two hundred ventricles were manually dissected from L3 stage larvae of Aniskakis simplex s.s. to allow direct protein analysis. Denaturing gel electrophoresis followed by monochromatic silver staining which revealed the presence of differential (enriched) proteins when compared to total nematode extracts. Such comparison was performed by means of 1D and 2D electrophoresis. Pooled antisera from Anisakis spp.-allergic patients were used in western blots revealing the presence of 13 immunoreactive bands in the ventricular extracts in 1D, with 82 spots revealed in 2D. The corresponding protein bands and spots were excised from the silver-stained gel and protein assignation was made by MALDI-TOF\/TOF. A total of 13 (including proteoforms) were unambiguously identified. The majority of these proteins are known to be secreted by nematodes into the external environment, of which three are described as being major allergens in other organisms with different phylogenetic origin and one is an Anisakis simplex allergen.<\/jats:p>","DOI":"10.3390\/genes11060683","type":"journal-article","created":{"date-parts":[[2020,6,24]],"date-time":"2020-06-24T07:14:19Z","timestamp":1592982859000},"page":"683","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Immunoreactive Proteins in the Esophageal Gland Cells of Anisakis Simplex Sensu Stricto Detected by MALDI-TOF\/TOF Analysis"],"prefix":"10.3390","volume":"11","author":[{"given":"Lee","family":"Robertson","sequence":"first","affiliation":[{"name":"Department of Biodiversity and Evolutionary Biology, Museo Nacional de Ciencias Naturales, CSIC, calle Jos\u00e9 Guti\u00e9rrez Abascal 2, 28006 Madrid, Spain"},{"name":"Departamento de Protecci\u00f3n Vegetal, INIA, Ctra La Coru\u00f1a Km 7\u20325, 28040 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Susana","family":"C. Arcos","sequence":"additional","affiliation":[{"name":"Department of Biodiversity and Evolutionary Biology, Museo Nacional de Ciencias Naturales, CSIC, calle Jos\u00e9 Guti\u00e9rrez Abascal 2, 28006 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5726-853X","authenticated-orcid":false,"given":"Sergio","family":"Ciordia","sequence":"additional","affiliation":[{"name":"Unidad de Prote\u00f3mica Centro Nacional de Biotecnolog\u00eda, CSIC, calle Darwin 3, Campus de Cantoblanco, 28049 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Noelia","family":"Carballeda-Sanguiao","sequence":"additional","affiliation":[{"name":"Instituto de Ciencia y Tecnolog\u00eda de Alimentos y Nutrici\u00f3n, CSIC, Calle Jos\u00e9 Antonio Novais, 10, 28040 Madrid, Spain"},{"name":"Servicio de Immunolog\u00eda, Hospital Universitario La Paz. Paseo de la Castellana, 261, 28046 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mar\u00eda del Carmen","family":"Mena","sequence":"additional","affiliation":[{"name":"Unidad de Prote\u00f3mica Centro Nacional de Biotecnolog\u00eda, CSIC, calle Darwin 3, Campus de Cantoblanco, 28049 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0003-2080","authenticated-orcid":false,"given":"Isabel","family":"S\u00e1nchez-Alonso","sequence":"additional","affiliation":[{"name":"Instituto de Ciencia y Tecnolog\u00eda de Alimentos y Nutrici\u00f3n, CSIC, Calle Jos\u00e9 Antonio Novais, 10, 28040 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3957-4955","authenticated-orcid":false,"given":"Miguel","family":"Gonzalez-Mu\u00f1oz","sequence":"additional","affiliation":[{"name":"Servicio de Immunolog\u00eda, Hospital Universitario La Paz. Paseo de la Castellana, 261, 28046 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mercedes","family":"Careche","sequence":"additional","affiliation":[{"name":"Instituto de Ciencia y Tecnolog\u00eda de Alimentos y Nutrici\u00f3n, CSIC, Calle Jos\u00e9 Antonio Novais, 10, 28040 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alfonso","family":"Navas","sequence":"additional","affiliation":[{"name":"Department of Biodiversity and Evolutionary Biology, Museo Nacional de Ciencias Naturales, CSIC, calle Jos\u00e9 Guti\u00e9rrez Abascal 2, 28006 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2020,6,22]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"311","DOI":"10.1157\/13077114","article-title":"Anisakiasis gastrointestinal manifestations: Description of 42 cases","volume":"205","year":"2005","journal-title":"Rev. Clin. 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