{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T21:33:03Z","timestamp":1790371983502,"version":"4.1.0"},"reference-count":30,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2020,6,24]],"date-time":"2020-06-24T00:00:00Z","timestamp":1592956800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100018982","name":"Fondazione Bambino Ges\u00f9","doi-asserted-by":"publisher","award":["201903_SBG"],"award-info":[{"award-number":["201903_SBG"]}],"id":[{"id":"10.13039\/501100018982","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003196","name":"Ministero della Salute","doi-asserted-by":"publisher","award":["Ricerca Corrente 201905_genetica"],"award-info":[{"award-number":["Ricerca Corrente 201905_genetica"]}],"id":[{"id":"10.13039\/501100003196","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Genes"],"abstract":"<jats:p>Anisakiasis is nowadays a well-known infection, mainly caused by the accidental ingestion of Anisakis larvae, following the consumption of raw or undercooked fishes and cephalopods. Due to the similarity of symptoms with those of common gastrointestinal disorders, this infection is often underestimated, and the need for new specific diagnostic tools is becoming crucial. Given the remarkable impact that MALDI\u2013TOF MS biotyping had in the last decade in clinical routine practice for the recognition of bacterial and fungi strains, a similar scenario could be foreseen for the identification of parasites, such as nematodes. In this work, a MALDI\u2013TOF MS profiling of Anisakis proteome was pursued with a view to constructing a first spectral library for the diagnosis of Anisakis infections. At the same time, a shotgun proteomics approach by LC\u2013ESI\u2013MS\/MS was performed on the two main fractions obtained from protein extraction, to evaluate the protein species enriched by the protocol. A set of MALDI\u2013TOF MS signals associated with proteins originating in the ribosomal fraction of the nematode extract was selected as a potential diagnostic tool for the identification of Anisakis spp.<\/jats:p>","DOI":"10.3390\/genes11060693","type":"journal-article","created":{"date-parts":[[2020,6,24]],"date-time":"2020-06-24T08:54:50Z","timestamp":1592988890000},"page":"693","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":32,"title":["Mass Spectrometry Based-Proteomic Analysis of Anisakis spp.: A Preliminary Study towards a New Diagnostic Tool"],"prefix":"10.3390","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6364-2121","authenticated-orcid":false,"given":"Valeria","family":"Marzano","sequence":"first","affiliation":[{"name":"Unit of Human Microbiome, Bambino Ges\u00f9 Children\u2019s Hospital IRCCS, Piazza Sant\u2019Onofrio 4, 00165 Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8936-895X","authenticated-orcid":false,"given":"Stefania","family":"Pane","sequence":"additional","affiliation":[{"name":"Unit of Parasitology, Bambino Ges\u00f9 Children\u2019s Hospital IRCCS, Piazza Sant\u2019Onofrio 4, 00165 Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gianluca","family":"Foglietta","sequence":"additional","affiliation":[{"name":"Unit of Parasitology, Bambino Ges\u00f9 Children\u2019s Hospital IRCCS, Piazza Sant\u2019Onofrio 4, 00165 Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stefano","family":"Levi Mortera","sequence":"additional","affiliation":[{"name":"Unit of Human Microbiome, Bambino Ges\u00f9 Children\u2019s Hospital IRCCS, Piazza Sant\u2019Onofrio 4, 00165 Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pamela","family":"Vernocchi","sequence":"additional","affiliation":[{"name":"Unit of Human Microbiome, Bambino Ges\u00f9 Children\u2019s Hospital IRCCS, Piazza Sant\u2019Onofrio 4, 00165 Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrea","family":"Onetti Muda","sequence":"additional","affiliation":[{"name":"Department of Laboratories, Bambino Ges\u00f9 Children\u2019s Hospital IRCCS, Piazza Sant\u2019Onofrio 4, 00165 Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0134-2830","authenticated-orcid":false,"given":"Lorenza","family":"Putignani","sequence":"additional","affiliation":[{"name":"Units of Parasitology and Human Microbiome, Bambino Ges\u00f9 Children\u2019s Hospital IRCCS, Piazza Sant\u2019Onofrio 4, 00165 Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2020,6,24]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"455","DOI":"10.1007\/s11882-014-0455-3","article-title":"Allergic Reactions to Anisakis Found in Fish","volume":"14","author":"Nieuwenhuizen","year":"2014","journal-title":"Curr. Allergy Asthma Rep."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"548","DOI":"10.1111\/pim.12349","article-title":"Anisakis\u2014Immunology of a foodborne parasitosis","volume":"38","author":"Nieuwenhuizen","year":"2016","journal-title":"Parasite Immunol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"806","DOI":"10.1086\/656238","article-title":"Anisakidosis: Perils of the Deep","volume":"51","author":"Hochberg","year":"2010","journal-title":"Clin. Infect. Dis."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"620","DOI":"10.1039\/C0MB00138D","article-title":"MALDI-TOF mass spectrometry proteomic phenotyping of clinically relevant fungi","volume":"7","author":"Putignani","year":"2011","journal-title":"Mol. Biosyst."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"3314","DOI":"10.1016\/j.jprot.2012.03.048","article-title":"MALDI-TOF MS proteomic phenotyping of filamentous and other fungi from clinical origin","volume":"75","author":"Masotti","year":"2012","journal-title":"J. Proteom."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1016\/j.jprot.2013.10.013","article-title":"Proteomics boosts translational and clinical microbiology","volume":"97","author":"Petrucca","year":"2014","journal-title":"J. Proteom."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"683","DOI":"10.1080\/14789450.2018.1505510","article-title":"Applications of MALDI-TOF mass spectrometry in clinical proteomics","volume":"15","author":"Greco","year":"2018","journal-title":"Expert Rev. Proteom."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"184","DOI":"10.3389\/fcimb.2017.00184","article-title":"MALDI-TOF MS Profiling-Advances in Species Identification of Pests, Parasites, and Vectors","volume":"7","author":"Murugaiyan","year":"2017","journal-title":"Front. Cell. Infect. Microbiol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"245","DOI":"10.1186\/s13071-019-3493-9","article-title":"MALDI-TOF mass spectrometry as a diagnostic tool in human and veterinary helminthology: A systematic review","volume":"12","author":"Feucherolles","year":"2019","journal-title":"Parasites Vectors"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1016\/j.ijid.2019.02.047","article-title":"The application of proteomic methods (MALDI-toff MS) for studying protein profiles of some nematodes (dirofilaria and ascaris) for differentiating species","volume":"82","author":"Nagorny","year":"2019","journal-title":"Int. J. Infect. Dis."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"795","DOI":"10.1038\/nprot.2016.042","article-title":"Label-free quantification in ion mobility-enhanced data-independent acquisition proteomics","volume":"11","author":"Distler","year":"2016","journal-title":"Nat. Protoc."},{"key":"ref_12","unstructured":"(2020, May 21). g:Profiler. Available online: https:\/\/biit.cs.ut.ee\/gprofiler\/orth."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"W83","DOI":"10.1093\/nar\/gkw199","article-title":"g:Profiler\u2014A web server for functional interpretation of gene lists (2016 update)","volume":"44","author":"Reimand","year":"2016","journal-title":"Nucleic Acids Res."},{"key":"ref_14","unstructured":"(2020, May 20). STRING. Available online: https:\/\/string-db.org\/."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"D607","DOI":"10.1093\/nar\/gky1131","article-title":"STRING v11: Protein\u2013protein association networks with increased coverage, supporting functional discovery in genome-wide experimental datasets","volume":"47","author":"Szklarczyk","year":"2019","journal-title":"Nucleic Acids Res."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.jprot.2019.04.006","article-title":"Proteome profiling of L3 and L4 Anisakis simplex development stages by TMT-based quantitative proteomics","volume":"201","author":"Mateos","year":"2019","journal-title":"J. Proteom."},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Holmes, D.T., Romney, M.G., Angel, P., and DeMarco, M.L. (2020). Proteomic Applications in Pathology and Laboratory Medicine: Present State and Future Prospects. Clin. Biochem., S0009912020300813.","DOI":"10.1016\/j.clinbiochem.2020.05.007"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"185","DOI":"10.1093\/mmy\/myw038","article-title":"Performance assessment of two lysis methods for direct identification of yeasts from clinical blood cultures using MALDI-TOF mass spectrometry","volume":"55","author":"Jeddi","year":"2017","journal-title":"Med. Myco."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"2133","DOI":"10.1007\/s10096-019-03654-4","article-title":"Comparison of rapid BACpro\u00ae II, Sepsityper\u00ae kit and in-house preparation methods for direct identification of bacteria from blood cultures by MALDI-TOF MS with and without Sepsityper\u00ae module analysis","volume":"38","author":"Kayin","year":"2019","journal-title":"Eur. J. Clin. Microbiol. Infect. Dis."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"675","DOI":"10.1373\/jalm.2018.027318","article-title":"The Use of Matrix-Assisted Laser Desorption\/Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF MS) for the Identification of Pathogens Causing Sepsis","volume":"3","author":"Luethy","year":"2019","journal-title":"J. Appl. Lab. Med."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"746","DOI":"10.1021\/ac0008791","article-title":"Characterization of the protein subset desorbed by MALDI from whole bacterial cells","volume":"73","author":"Ryzhov","year":"2001","journal-title":"Anal. Chem."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1186\/s13071-017-2585-7","article-title":"Tissue-specific transcriptomes of Anisakis simplex (sensu stricto) and Anisakis pegreffii reveal potential molecular mechanisms involved in pathogenicity","volume":"11","author":"Cavallero","year":"2018","journal-title":"Parasites Vectors"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"376","DOI":"10.1007\/s11882-009-0055-9","article-title":"Allergen databases: Current status and perspectives","volume":"9","author":"Mari","year":"2009","journal-title":"Curr. Allergy Asthma Rep."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"384","DOI":"10.1016\/j.ijppaw.2019.04.007","article-title":"Anisakis Nematodes in Fish and Shellfish- from infection to allergies","volume":"9","author":"Aibinu","year":"2019","journal-title":"Int. J. Parasitol. Parasites Wildl."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"360","DOI":"10.1128\/CMR.00012-07","article-title":"Anisakis simplex: From Obscure Infectious Worm to Inducer of Immune Hypersensitivity","volume":"21","author":"Audicana","year":"2008","journal-title":"CMR"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"1233","DOI":"10.1007\/s00436-006-0396-4","article-title":"Molecular cloning and expression of two new allergens from Anisakis simplex","volume":"100","author":"Kobayashi","year":"2007","journal-title":"Parasitol. Res."},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Garc\u00eda-Mayoral, M.F., Trevi\u00f1o, M.A., P\u00e9rez-Pi\u00f1ar, T., Caballero, M.L., Knaute, T., Umpierrez, A., Bruix, M., and Rodr\u00edguez-P\u00e9rez, R. (2014). Relationships between IgE\/IgG4 Epitopes, Structure and Function in Anisakis simplex Ani s 5, a Member of the SXP\/RAL-2 Protein Family. PLoS Negl. Trop. Dis., 8.","DOI":"10.1371\/journal.pntd.0002735"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"42","DOI":"10.1186\/s13028-017-0310-3","article-title":"Excretory\/secretory products of anisakid nematodes: Biological and pathological roles","volume":"59","author":"Mehrdana","year":"2017","journal-title":"Acta Vet. Scand."},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"\u0141opie\u0144ska-Biernat, E., Stryi\u0144ski, R., Dmitryjuk, M., and Wasilewska, B. (2019). Infective larvae of Anisakis simplex (Nematoda) accumulate trehalose and glycogen in response to starvation and temperature stress. Biol. Open, 8.","DOI":"10.1242\/bio.040014"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"24","DOI":"10.1016\/j.molbiopara.2018.10.002","article-title":"Comparative transcriptome analyses of the third and fourth stage larvae of Anisakis simplex (Nematoda: Anisakidae)","volume":"226","author":"Kim","year":"2018","journal-title":"Mol. Biochem. Parasitol."}],"container-title":["Genes"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2073-4425\/11\/6\/693\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T09:42:17Z","timestamp":1760175737000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2073-4425\/11\/6\/693"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,6,24]]},"references-count":30,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2020,6]]}},"alternative-id":["genes11060693"],"URL":"https:\/\/doi.org\/10.3390\/genes11060693","relation":{},"ISSN":["2073-4425"],"issn-type":[{"value":"2073-4425","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,6,24]]}}}