{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T23:34:04Z","timestamp":1791934444211,"version":"4.3.4"},"reference-count":159,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2020,6,29]],"date-time":"2020-06-29T00:00:00Z","timestamp":1593388800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000038","name":"Natural Sciences and Engineering Research Council of Canada","doi-asserted-by":"publisher","award":["Discovery Grant RGPIN-2017-04480"],"award-info":[{"award-number":["Discovery Grant RGPIN-2017-04480"]}],"id":[{"id":"10.13039\/501100000038","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000196","name":"Canada Foundation for Innovation","doi-asserted-by":"publisher","award":["37324"],"award-info":[{"award-number":["37324"]}],"id":[{"id":"10.13039\/501100000196","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Brazilian National Council for Scientific and Technological Development","award":["310640\/2017-2"],"award-info":[{"award-number":["310640\/2017-2"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Genes"],"abstract":"<jats:p>Leishmaniasis (Leishmania species), sleeping sickness (Trypanosoma brucei), and Chagas disease (Trypanosoma cruzi) are devastating and globally spread diseases caused by trypanosomatid parasites. At present, drugs for treating trypanosomatid diseases are far from ideal due to host toxicity, elevated cost, limited access, and increasing rates of drug resistance. Technological advances in parasitology, chemistry, and genomics have unlocked new possibilities for novel drug concepts and compound screening technologies that were previously inaccessible. In this perspective, we discuss current models used in drug-discovery cascades targeting trypanosomatids (from in vitro to in vivo approaches), their use and limitations in a biological context, as well as different examples of recently discovered lead compounds.<\/jats:p>","DOI":"10.3390\/genes11070722","type":"journal-article","created":{"date-parts":[[2020,6,30]],"date-time":"2020-06-30T14:27:29Z","timestamp":1593527249000},"page":"722","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":50,"title":["Of Drugs and Trypanosomatids: New Tools and Knowledge to Reduce Bottlenecks in Drug Discovery"],"prefix":"10.3390","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6501-9229","authenticated-orcid":false,"given":"Arijit","family":"Bhattacharya","sequence":"first","affiliation":[{"name":"Department of Microbiology, Adamas University, Kolkata, West Bengal 700 126, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Audrey","family":"Corbeil","sequence":"additional","affiliation":[{"name":"Department of Pathology and Microbiology, Faculty of Veterinary Medicine, Universit\u00e9 de Montr\u00e9al, Saint-Hyacinthe, QC J2S 2M2, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4688-2462","authenticated-orcid":false,"given":"Rubens L.","family":"do Monte-Neto","sequence":"additional","affiliation":[{"name":"Instituto Ren\u00e9 Rachou, Funda\u00e7\u00e3o Oswaldo Cruz, Belo Horizonte MG 30190-009, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4834-4956","authenticated-orcid":false,"given":"Christopher","family":"Fernandez-Prada","sequence":"additional","affiliation":[{"name":"Department of Pathology and Microbiology, Faculty of Veterinary Medicine, Universit\u00e9 de Montr\u00e9al, Saint-Hyacinthe, QC J2S 2M2, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2020,6,29]]},"reference":[{"key":"ref_1","unstructured":"Roy, K. 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