{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T04:43:47Z","timestamp":1787028227045,"version":"build-2736575974"},"reference-count":112,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2021,2,1]],"date-time":"2021-02-01T00:00:00Z","timestamp":1612137600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004794","name":"Centre National de la Recherche Scientifique","doi-asserted-by":"publisher","award":["11-LABX-0011-01"],"award-info":[{"award-number":["11-LABX-0011-01"]}],"id":[{"id":"10.13039\/501100004794","id-type":"DOI","asserted-by":"publisher"}]},{"name":"PHC Utique","award":["37063TG"],"award-info":[{"award-number":["37063TG"]}]},{"name":"PHC-Utique","award":["17G 0820"],"award-info":[{"award-number":["17G 0820"]}]},{"name":"Ministry of Higher Education and Scientific Research","award":["LR16IPT04"],"award-info":[{"award-number":["LR16IPT04"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Genes"],"abstract":"<jats:p>DEAD-box RNA helicases are ubiquitous proteins found in all kingdoms of life and that are associated with all processes involving RNA. Their central roles in biology make these proteins potential targets for therapeutic or prophylactic drugs. The Ded1\/DDX3 subfamily of DEAD-box proteins is of particular interest because of their important role(s) in translation. In this paper, we identified and aligned the protein sequences of 28 different DEAD-box proteins from the kinetoplast-protozoan parasite Leishmania infantum, which is the cause of the visceral form of leishmaniasis that is often lethal if left untreated, and compared them with the consensus sequence derived from DEAD-box proteins in general, and from the Ded1\/DDX3 subfamily in particular, from a wide variety of other organisms. We identified three potential homologs of the Ded1\/DDX3 subfamily and the equivalent proteins from the related protozoan parasite Trypanosoma brucei, which is the causative agent of sleeping sickness. We subsequently tested these proteins for their ability to complement a yeast strain deleted for the essential DED1 gene. We found that the DEAD-box proteins from Trypanosomatids are highly divergent from other eukaryotes, and consequently they are suitable targets for protein-specific drugs.<\/jats:p>","DOI":"10.3390\/genes12020212","type":"journal-article","created":{"date-parts":[[2021,2,1]],"date-time":"2021-02-01T11:40:48Z","timestamp":1612179648000},"page":"212","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["The In Silico Identification of Potential Members of the Ded1\/DDX3 Subfamily of DEAD-Box RNA Helicases from the Protozoan Parasite Leishmania infantum and Their Analyses in Yeast"],"prefix":"10.3390","volume":"12","author":[{"given":"Molka","family":"Mokdadi","sequence":"first","affiliation":[{"name":"Expression G\u00e9n\u00e9tique Microbienne, UMR8261 CNRS, Universit\u00e9 de Paris, Institut de Biologie Physico-Chimique, 13 rue Pierre et Marie Curie, 75005 Paris, France"},{"name":"PSL Research University, 75005 Paris, France"},{"name":"Laboratory of Molecular Epidemiology and Experimental Pathology (LR16IPT04), Institut Pasteur de Tunis, Universit\u00e9 de Tunis El Manar, 13 Place Pasteur, BP74 Tunis-Belv\u00e9d\u00e8re 1002, Tunisia"},{"name":"Institut National des Sciences Appliqu\u00e9es et Technologies, Universit\u00e9 de Carthage, CEDEX, Tunis 1080, Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7855-9400","authenticated-orcid":false,"given":"Yosser Zina","family":"Abdelkrim","sequence":"additional","affiliation":[{"name":"Expression G\u00e9n\u00e9tique Microbienne, UMR8261 CNRS, Universit\u00e9 de Paris, Institut de Biologie Physico-Chimique, 13 rue Pierre et Marie Curie, 75005 Paris, France"},{"name":"Laboratory of Molecular Epidemiology and Experimental Pathology (LR16IPT04), Institut Pasteur de Tunis, Universit\u00e9 de Tunis El Manar, 13 Place Pasteur, BP74 Tunis-Belv\u00e9d\u00e8re 1002, Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Josette","family":"Banroques","sequence":"additional","affiliation":[{"name":"Expression G\u00e9n\u00e9tique Microbienne, UMR8261 CNRS, Universit\u00e9 de Paris, Institut de Biologie Physico-Chimique, 13 rue Pierre et Marie Curie, 75005 Paris, France"},{"name":"PSL Research University, 75005 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emmeline","family":"Huvelle","sequence":"additional","affiliation":[{"name":"Expression G\u00e9n\u00e9tique Microbienne, UMR8261 CNRS, Universit\u00e9 de Paris, Institut de Biologie Physico-Chimique, 13 rue Pierre et Marie Curie, 75005 Paris, France"},{"name":"PSL Research University, 75005 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rafeh","family":"Oualha","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Epidemiology and Experimental Pathology (LR16IPT04), Institut Pasteur de Tunis, Universit\u00e9 de Tunis El Manar, 13 Place Pasteur, BP74 Tunis-Belv\u00e9d\u00e8re 1002, Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hilal","family":"Yeter-Alat","sequence":"additional","affiliation":[{"name":"Expression G\u00e9n\u00e9tique Microbienne, UMR8261 CNRS, Universit\u00e9 de Paris, Institut de Biologie Physico-Chimique, 13 rue Pierre et Marie Curie, 75005 Paris, France"},{"name":"PSL Research University, 75005 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2763-8963","authenticated-orcid":false,"given":"Ikram","family":"Guizani","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Epidemiology and Experimental Pathology (LR16IPT04), Institut Pasteur de Tunis, Universit\u00e9 de Tunis El Manar, 13 Place Pasteur, BP74 Tunis-Belv\u00e9d\u00e8re 1002, Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mourad","family":"Barhoumi","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Epidemiology and Experimental Pathology (LR16IPT04), Institut Pasteur de Tunis, Universit\u00e9 de Tunis El Manar, 13 Place Pasteur, BP74 Tunis-Belv\u00e9d\u00e8re 1002, Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"N. Kyle","family":"Tanner","sequence":"additional","affiliation":[{"name":"Expression G\u00e9n\u00e9tique Microbienne, UMR8261 CNRS, Universit\u00e9 de Paris, Institut de Biologie Physico-Chimique, 13 rue Pierre et Marie Curie, 75005 Paris, France"},{"name":"PSL Research University, 75005 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2021,2,1]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Akhoundi, M., Kuhls, K., Cannet, A., Vot\u00fdpka, J., Marty, P., Delaunay, P., and Sereno, D. (2016). Correction: A Historical Overview of the Classification, Evolution, and Dispersion of Leishmania Parasites and Sandflies. PLoS Negl. Trop. Dis., 10.","DOI":"10.1371\/journal.pntd.0004349"},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Akhoundi, M., Kuhls, K., Cannet, A., Vot\u00fdpka, J., Marty, P., Delaunay, P., and Sereno, D. (2016). 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