{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,12]],"date-time":"2025-10-12T02:02:22Z","timestamp":1760234542070,"version":"build-2065373602"},"reference-count":45,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2021,5,25]],"date-time":"2021-05-25T00:00:00Z","timestamp":1621900800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","award":["400797\/2014-3"],"award-info":[{"award-number":["400797\/2014-3"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Genes"],"abstract":"<jats:p>Susceptibility to diseases is inherited and can be transmitted between populations. Single-nucleotide polymorphism (SNPs) in genes related to immune response is associated with diseases in cattle. This study investigated SNPs in the genomic region of cytokines in 702 samples of Curraleiro P\u00e9-Duro cattle and associated them with the occurrence of antibodies in brucellosis, leptospirosis, neosporosis, leukosis, infectious bovine rhinotracheitis (IBR), and bovine viral diarrhea (BVD) tests. DNA samples were evaluated by the kompetitive allele-specific polymerase chain reaction (KASP) method to identify polymorphisms. The gametic phase and SNP haplotypes were determined with the help of PHASE 2.1.1 software. Haplotypes were associated with serological results against Brucella abortus, Leptospira sp., Neospora caninum, leukosis, infectious rhinotracheitis, and BVD using univariate analysis followed by logistic regression. Haplotype 2 of TLR2 was present in 70% of the animals that tested positive for N. caninum infection. Haplotypes of TLR10 and TLR6 and IL10RA were more common in seronegative animals. Haplotypes related to the gene IL10RA were associated with animals negative to all infections. Curraleiro P\u00e9-Duro cattle presented polymorphisms related to resistance to bacterial, viral, and N. caninum infections.<\/jats:p>","DOI":"10.3390\/genes12060806","type":"journal-article","created":{"date-parts":[[2021,5,25]],"date-time":"2021-05-25T13:14:21Z","timestamp":1621948461000},"page":"806","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Genomic Association between SNP Markers and Diseases in the \u201cCurraleiro P\u00e9-Duro\u201d Cattle"],"prefix":"10.3390","volume":"12","author":[{"given":"Thais Miranda Silva","family":"Freitas","sequence":"first","affiliation":[{"name":"School of Veterinary and Animal Science, Federal University of Goi\u00e1s, Campus Samambaia, Goi\u00e2nia, Goi\u00e1s 74690-900, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Juliana Moraes","family":"Dias","sequence":"additional","affiliation":[{"name":"School of Veterinary and Animal Science, Federal University of Goi\u00e1s, Campus Samambaia, Goi\u00e2nia, Goi\u00e1s 74690-900, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luanna Kim Pires","family":"Guimar\u00e3es","sequence":"additional","affiliation":[{"name":"School of Veterinary and Animal Science, Federal University of Goi\u00e1s, Campus Samambaia, Goi\u00e2nia, Goi\u00e1s 74690-900, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S\u00e1udio Vieira","family":"Peixoto","sequence":"additional","affiliation":[{"name":"School of Veterinary and Animal Science, Federal University of Goi\u00e1s, Campus Samambaia, Goi\u00e2nia, Goi\u00e1s 74690-900, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rayanne Henrique Santana da","family":"Silva","sequence":"additional","affiliation":[{"name":"School of Veterinary and Animal Science, Federal University of Goi\u00e1s, Campus Samambaia, Goi\u00e2nia, Goi\u00e1s 74690-900, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kareem Rady","family":"Badr","sequence":"additional","affiliation":[{"name":"Environmental Virology Laboratory, Water Pollution Research Department, National Research Centre, Dokki, Giza 12622, Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria Ivete","family":"Moura","sequence":"additional","affiliation":[{"name":"Course in Veterinary Medicine, Pontifical Catholic University of Goi\u00e1s, Av. Engler, Jardim Mariliza, Goi\u00e2nia, Goi\u00e1s 74885-460, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2712-4789","authenticated-orcid":false,"given":"Adriana Santana do","family":"Carmo","sequence":"additional","affiliation":[{"name":"School of Veterinary and Animal Science, Federal University of Goi\u00e1s, Campus Samambaia, Goi\u00e2nia, Goi\u00e1s 74690-900, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1385-8439","authenticated-orcid":false,"given":"Vincenzo","family":"Landi","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine, University of Bari Aldo Moro, Str. Prov. per Casamassima, Km 3, 70010 Valenzano, BA, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4993-5523","authenticated-orcid":false,"given":"Maria Clorinda Soares","family":"Fioravanti","sequence":"additional","affiliation":[{"name":"School of Veterinary and Animal Science, Federal University of Goi\u00e1s, Campus Samambaia, Goi\u00e2nia, Goi\u00e1s 74690-900, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2021,5,25]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1017\/S2078633610001244","article-title":"Conservaci\u00f3n del bovino Curraleiro: Cuantificaci\u00f3n del censo y caracterizaci\u00f3n de los criadores","volume":"48","author":"Fioravanti","year":"2011","journal-title":"Anim. Genet. Resour."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1403","DOI":"10.1590\/S0100-736X2013001200002","article-title":"Cellular immune response of Curraleiro P\u00e9-duro and Nellore calves following Mycobacterium bovis-BCG vaccination","volume":"33","author":"Maggioli","year":"2013","journal-title":"Pesq Vet. Bras."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"339","DOI":"10.1590\/S0100-736X2009000400011","article-title":"Immunophenotyping and quantitative evaluation of circulating lymphocytes of cattle of the Curraleiro breed","volume":"29","author":"Moraes","year":"2009","journal-title":"Pesq. Vet. Bras."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"338","DOI":"10.21071\/az.v69i267.5353","article-title":"Resistencia y resiliencia a las enfermedades en las razas de rumiantes locales: Un enfoque en Am\u00e9rica del Sur","volume":"69","author":"Fioravanti","year":"2020","journal-title":"Arch. Zootec."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"1846","DOI":"10.1128\/IAI.74.3.1846-1856.2006","article-title":"Role of tumor necrosis factor \u03b1 (TNF-\u03b1) and interleukin-10 in the pathogenesis of severe murine monocytotropic ehrlichiosis: Increased resistance of TNF receptor p55- and p75-deficient mice to fatal ehrlichial infection","volume":"74","author":"Ismail","year":"2006","journal-title":"Infect. Immun."},{"key":"ref_6","first-page":"40","article-title":"Marcadores gen\u00e9ticos para resistencia y susceptibilidad a enfermedades infecciosas en animales dom\u00e9sticos","volume":"25","author":"Ripoli","year":"2005","journal-title":"Analecta Vet."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"956","DOI":"10.1101\/gr.6.10.956","article-title":"Bovine natural resistance associated macrophage protein 1 (Nramp1) gene","volume":"6","author":"Feng","year":"1996","journal-title":"Genome Res."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"197","DOI":"10.1111\/j.1365-2052.1986.tb03191.x","article-title":"Evidence for BoLA-linked resistance and susceptibility to subclinical progression of bovine leukaemia virus infection","volume":"17","author":"Lewin","year":"1986","journal-title":"Anim. 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Immunopathol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"1062","DOI":"10.1007\/s003359901162","article-title":"Single nucleotide polymorphism (SNP) discovery and linkage mapping of bovine cytokine genes","volume":"10","author":"Grosse","year":"1999","journal-title":"Mamm. Genome"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"7","DOI":"10.4067\/S0301-732X2006000100002","article-title":"Brucella abortus: Inmunidad, vacunas y estrategias de prevenci\u00f3n basadas en \u00e1cidos nucleicos","volume":"38","author":"Rivers","year":"2006","journal-title":"Arch. Med. Vet."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"1033","DOI":"10.1128\/IAI.70.3.1033-1041.2002","article-title":"Toll receptors: A central element in innate immune responses","volume":"70","author":"Vasselon","year":"2002","journal-title":"Infect. Immun."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"151","DOI":"10.1073\/pnas.0913006107","article-title":"Diversity and evolution of 11 innate immune genes in Bos taurus taurus and Bos taurus indicus cattle","volume":"107","author":"Seabury","year":"2010","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1016\/S1286-4579(00)01350-2","article-title":"Immunity and protection against Brucella abortus","volume":"3","author":"Golding","year":"2001","journal-title":"Microbes. Infect."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"8","DOI":"10.4322\/rbpv.01801002","article-title":"Emprego da RT-PCR em tempo real para a quantifica\u00e7\u00e3o da express\u00e3o de genes associados \u00e0 resposta imune em bezerros bovinos experimentalmente infectados por Neospora caninum","volume":"18","author":"Nishi","year":"2009","journal-title":"Rev. Bras. Parasitol. Vet."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"39530","DOI":"10.1038\/srep39530","article-title":"Leptospira surface adhesin (Lsa21) induces Toll like receptor 2 and 4 mediated inflammatory responses in macrophages","volume":"6","author":"Faisal","year":"2016","journal-title":"Sci. Rep."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"2650","DOI":"10.1128\/jvi.70.4.2650-2653.1996","article-title":"Cytokine regulation by virus infection: Bovine viral diarrhea virus, a flavivirus, downregulates production of tumor necrosis factor \u03b1 in macrophages in vitro","volume":"70","author":"Adler","year":"1996","journal-title":"J. Virol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1016\/j.rvsc.2020.01.012","article-title":"Comparative humoral immune response against cytopathic or non-cytopathic bovine viral diarrhea virus infection","volume":"129","author":"Rajput","year":"2020","journal-title":"Res. Vet. Sci."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"923","DOI":"10.1128\/JVI.76.2.923-927.2002","article-title":"\u03b1\/\u03b2 and \u03b3 interferons are induced by infection with noncytopathic bovine viral diarrhea virus in vivo","volume":"76","author":"Charleston","year":"2002","journal-title":"J. Virol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"190","DOI":"10.1016\/j.livsci.2014.04.034","article-title":"Genomics and disease resistance studies in livestock","volume":"166","author":"Bishop","year":"2014","journal-title":"Livest. Sci."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"189","DOI":"10.1016\/j.prevetmed.2009.05.020","article-title":"Candidate gene polymorphisms (BoIFNG, TLR4, SLC11A1) as risk factors for paratuberculosis infection in cattle","volume":"91","author":"Pinedo","year":"2009","journal-title":"Prev. Vet. Med."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"507","DOI":"10.3389\/fgene.2018.00507","article-title":"Exploring the Impact of Single-Nucleotide Polymorphisms on Translation","volume":"9","author":"Robert","year":"2018","journal-title":"Front. Genet."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1590\/S1516-89132014000100013","article-title":"SNPs genotyping technologies and their applications in farm animals breeding programs: Review","volume":"57","author":"Koopaee","year":"2014","journal-title":"Braz. Arch. Biol. Technol."},{"key":"ref_26","doi-asserted-by":"crossref","unstructured":"International HapMap Consortium (2005). A haplotype map of the human genome. Nature, 437, 1299\u20131320.","DOI":"10.1038\/nature04226"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"1215","DOI":"10.1093\/nar\/16.3.1215","article-title":"A simple salting out procedure for extracting DNA from human nucleated cells","volume":"16","author":"Miller","year":"1988","journal-title":"Nucleic Acids Res."},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"Porto Neto, L.R., Bunch, R.J., Harrison, B.E., Prayaga, K.C., and Barendse, W. (2010). Haplotypes that include the integrin \u03b1 11 gene are associated with tick burden in cattle. BMC Genet., 11.","DOI":"10.1186\/1471-2156-11-55"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"421","DOI":"10.1007\/s10911-011-9238-9","article-title":"Gene Polymorphisms: The Keys for Marker Assisted Selection and Unraveling Core Regulatory Pathways for Mastitis Resistance","volume":"16","author":"Pighetti","year":"2011","journal-title":"J. Mammary Gland Biol. Neoplasia"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"24","DOI":"10.1186\/s12977-017-0348-3","article-title":"Single nucleotide polymorphisms in the bovine MHC region of Japanese Black cattle are associated with bovine leukemia virus proviral load","volume":"14","author":"Takeshima","year":"2017","journal-title":"Retrovirology"},{"key":"ref_31","unstructured":"Robinson, P., and Holme, D.J. (2018, March 16). KASP Version 4.0 SNP Genotyping Manual. Available online: https:\/\/www.cerealsdb.uk.net\/cerealgenomics\/CerealsDB\/PDFs\/KASP_SNP_Genotyping_Manual.pdf."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"9544","DOI":"10.1002\/ece3.3323","article-title":"Landscape genomic approach to detect selection signatures in locally adapted Brazilian swine genetic groups","volume":"7","author":"Cesconeto","year":"2017","journal-title":"Ecol. Evol."},{"key":"ref_33","first-page":"321327","article-title":"Genetic association analysis of paratuberculosis forms in holstein-friesian cattle","volume":"2014","author":"Garrido","year":"2014","journal-title":"Vet. Med. Int."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"978","DOI":"10.1086\/319501","article-title":"A New Statistical Method for Haplotype Reconstruction from Population Data","volume":"68","author":"Stephens","year":"2001","journal-title":"Am. J. Hum. Genet."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"449","DOI":"10.1086\/428594","article-title":"Accounting for Decay of Linkage Disequilibrium in Haplotype Inference and Missing-Data Imputation","volume":"76","author":"Stephens","year":"2005","journal-title":"Am. J. Hum. Genet."},{"key":"ref_36","unstructured":"R Development Core Team (2011). R: A Language and Environment for Statistical Computing, R Development Core Team. Available online: http:\/\/www.R-project.org."},{"key":"ref_37","doi-asserted-by":"crossref","unstructured":"Fisher, C.A., Bhattarai, E.K., Osterstock, J.B., Dowd, S.E., Seabury, P.M., Vikram, M., Whitlock, R.H., Schukken, Y.H., Schnabel, R.D., and Taylor, J.F. (2011). Evolution of the bovine TLR gene family and member associations with Mycobacterium avium subspecies paratuberculosis infection. PLoS ONE, 6.","DOI":"10.1371\/journal.pone.0027744"},{"key":"ref_38","doi-asserted-by":"crossref","unstructured":"Jann, O.C., King, A., Corrales, N.L., Anderson, S.I., Jensen, K., Ait-ali, T., Tang, H., Wu, C., Cockett, N.E., and Archibald, A.L. (2009). Comparative genomics of Toll-like receptor signalling in five species. BMC Genom., 10.","DOI":"10.1186\/1471-2164-10-216"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"449","DOI":"10.1111\/aji.12065","article-title":"Cutting-edge report: TLR10 plays a role in mediating bacterial peptidoglycan-induced trophoblast apoptosis","volume":"69","author":"Mulla","year":"2013","journal-title":"Am. J. Reprod. Immunol."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"4697","DOI":"10.1128\/iai.62.11.4697-4708.1994","article-title":"Interleukin-10 is expressed by bovine type 1 helper, type 2 helper, and unrestricted parasite-specific T-cell clones and inhibits proliferation of all three subsets in an accessory-cell-dependent manner","volume":"62","author":"Brown","year":"1994","journal-title":"Infect. Immun."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"260","DOI":"10.1590\/S0034-70942011000200014","article-title":"Citocinas e dor","volume":"61","author":"Oliveira","year":"2011","journal-title":"Rev. Bras. Anestesiol."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"30","DOI":"10.3389\/fimmu.2012.00030","article-title":"The cellular and molecular mechanisms of immuno-suppression by human type 1 regulatory T cells","volume":"3","author":"Gregori","year":"2012","journal-title":"Front. Immunol."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"4480","DOI":"10.1128\/IAI.05727-11","article-title":"Gene expression profiles of immune mediators and histopathological findings in animal models of leptospirosis: Comparison between susceptible hamsters and resistant mice","volume":"79","author":"Matsui","year":"2011","journal-title":"Infect. Immun."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"144","DOI":"10.1016\/j.cyto.2017.03.009","article-title":"High level of IL-10 expression in the blood of animal models possibly relates to resistance against leptospirosis","volume":"96","author":"Matsui","year":"2017","journal-title":"Cytokine"},{"key":"ref_45","first-page":"2737","article-title":"Bovine tuberculosis: The genetic basis of host susceptibility","volume":"277","author":"Allen","year":"2010","journal-title":"Proc. Biol. Sci."}],"container-title":["Genes"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2073-4425\/12\/6\/806\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T06:07:29Z","timestamp":1760162849000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2073-4425\/12\/6\/806"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,5,25]]},"references-count":45,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2021,6]]}},"alternative-id":["genes12060806"],"URL":"https:\/\/doi.org\/10.3390\/genes12060806","relation":{},"ISSN":["2073-4425"],"issn-type":[{"type":"electronic","value":"2073-4425"}],"subject":[],"published":{"date-parts":[[2021,5,25]]}}}