{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,22]],"date-time":"2026-01-22T14:13:08Z","timestamp":1769091188301,"version":"3.49.0"},"reference-count":80,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2021,6,9]],"date-time":"2021-06-09T00:00:00Z","timestamp":1623196800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32072706, 31772590, 31771375"],"award-info":[{"award-number":["32072706, 31772590, 31771375"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100012542","name":"Sichuan Province Science and Technology Support Program","doi-asserted-by":"publisher","award":["2019YJ0146, 2019YJ0021, 2019YJ0017"],"award-info":[{"award-number":["2019YJ0146, 2019YJ0021, 2019YJ0017"]}],"id":[{"id":"10.13039\/100012542","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Genes"],"abstract":"<jats:p>The serotonin (5-hydroxytryptamine, 5-HT) signaling system is involved in a variety of physiological functions, including the control of cognition, reward, learning, memory, and vasoconstriction in vertebrates. Contrary to the extensive studies in the mammalian system, little is known about the molecular characteristics of the avian serotonin signaling network. In this study, we cloned and characterized the full-length cDNA of three serotonin receptor genes (HTR1B, HTR1E and HTR1F) in chicken pituitaries. Synteny analyses indicated that HTR1B, HTR1E and HTR1F were highly conserved across vertebrates. Cell-based luciferase reporter assays showed that the three chicken HTRs were functional, capable of binding their natural ligands (5-HT) or selective agonists (CP94253, BRL54443, and LY344864) and inhibiting intracellular cAMP production in a dose-dependent manner. Moreover, activation of these receptors could stimulate the MAPK\/ERK signaling cascade. Quantitative real-time PCR analyses revealed that HTR1B, HTR1E and HTR1F were primarily expressed in various brain regions and the pituitary. In cultured chicken pituitary cells, we found that LY344864 could significantly inhibit the secretion of PRL stimulated by vasoactive intestinal peptide (VIP) or forskolin, revealing that HTR1F might be involved in the release of prolactin in chicken. Our findings provide insights into the molecular mechanism and facilitate a better understanding of the serotonergic modulation via HTR1B, HTR1E and HTR1F in avian species.<\/jats:p>","DOI":"10.3390\/genes12060891","type":"journal-article","created":{"date-parts":[[2021,6,9]],"date-time":"2021-06-09T14:16:04Z","timestamp":1623248164000},"page":"891","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Molecular Cloning and Functional Characterization of Three 5-HT Receptor Genes (HTR1B, HTR1E, and HTR1F) in Chickens"],"prefix":"10.3390","volume":"12","author":[{"given":"Caiyun","family":"Sun","sequence":"first","affiliation":[{"name":"Key Laboratory of Bio-Resources and Eco-Environment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu 610065, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yang","family":"Qiu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Bio-Resources and Eco-Environment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu 610065, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qin","family":"Ren","sequence":"additional","affiliation":[{"name":"Key Laboratory of Bio-Resources and Eco-Environment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu 610065, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiao","family":"Zhang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Bio-Resources and Eco-Environment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu 610065, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Baolong","family":"Cao","sequence":"additional","affiliation":[{"name":"Key Laboratory of Bio-Resources and Eco-Environment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu 610065, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yi","family":"Zou","sequence":"additional","affiliation":[{"name":"Key Laboratory of Bio-Resources and Eco-Environment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu 610065, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Juan","family":"Li","sequence":"additional","affiliation":[{"name":"Key Laboratory of Bio-Resources and Eco-Environment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu 610065, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7401-7401","authenticated-orcid":false,"given":"Jiannan","family":"Zhang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Bio-Resources and Eco-Environment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu 610065, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yajun","family":"Wang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Bio-Resources and Eco-Environment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu 610065, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2021,6,9]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"533","DOI":"10.1016\/S0091-3057(01)00746-8","article-title":"Molecular, pharmacological and functional diversity of 5-HT receptors","volume":"71","author":"Hoyer","year":"2002","journal-title":"Pharmacol. Biochem. Behav."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"187","DOI":"10.1111\/j.1365-2885.2008.00944.x","article-title":"Serotonin: A review","volume":"31","author":"Moses","year":"2008","journal-title":"Vet. Pharmacol. Ther."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"198","DOI":"10.1016\/j.bbr.2008.03.020","article-title":"Molecular biology of 5-HT receptors","volume":"195","author":"Hannon","year":"2008","journal-title":"Behav. Brain Res."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"791","DOI":"10.1016\/S1095-6433(00)00320-2","article-title":"Structure and function of invertebrate 5-HT receptors: A review","volume":"128","author":"Tierney","year":"2001","journal-title":"Comp. Biochem. Physiol. Part A Mol. Integr. Physiol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"3615","DOI":"10.2174\/138161206778522029","article-title":"5-HT3 receptors","volume":"12","author":"Thompson","year":"2006","journal-title":"Curr. Pharm. Des."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"706","DOI":"10.1038\/339706a0","article-title":"5-HT3 receptors are membrane ion channels","volume":"339","author":"Derkach","year":"1989","journal-title":"Nature"},{"key":"ref_7","first-page":"157","article-title":"International Union of Pharmacology classification of receptors for 5-hydroxytryptamine (Serotonin)","volume":"46","author":"Hoyer","year":"1994","journal-title":"Pharmacol. Rev."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"4017","DOI":"10.1002\/j.1460-2075.1991.tb04977.x","article-title":"Molecular cloning and characterization of a rat brain cDNA encoding a 5-hydroxytryptamine1B receptor","volume":"10","author":"Voigt","year":"1991","journal-title":"EMBO J."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"625","DOI":"10.1016\/S0079-6123(08)00929-1","article-title":"The roles of dopamine and serotonin, and of their receptors, in regulating sleep and waking","volume":"172","author":"Monti","year":"2008","journal-title":"Prog. Brain Res."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"360","DOI":"10.1124\/pr.59.07103","article-title":"5-HT receptor regulation of neurotransmitter release","volume":"59","author":"Fink","year":"2007","journal-title":"Pharmacol. Rev."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1016\/S0304-3940(97)00178-X","article-title":"Influence of 5-HT1B\/1D receptors on dopamine release in the guinea pig nucleus accumbens: A microdialysis study","volume":"225","author":"Magnusson","year":"1997","journal-title":"Neurosci. Lett."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"387","DOI":"10.1016\/0169-328X(95)00003-B","article-title":"Lack of genetically determined structural variants of the human serotonin-1E (5-HT1E) receptor protein points to its evolutionary conservation","volume":"29","author":"Erdmann","year":"1995","journal-title":"Mol. Brain Res."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"19761","DOI":"10.1016\/S0021-9258(19)88617-9","article-title":"Isolation of a mouse \u201c5HT1E-like\u201d serotonin receptor expressed predominantly in hippocampus","volume":"267","author":"Amlaiky","year":"1992","journal-title":"J. Biol. Chem."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"408","DOI":"10.1073\/pnas.90.2.408","article-title":"Cloning of another human serotonin receptor (5-HT1F): A fifth 5-HT1 receptor subtype coupled to the inhibition of adenylate cyclase","volume":"90","author":"Adham","year":"1993","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"173","DOI":"10.3389\/fneur.2016.00173","article-title":"Distribution of 5-HT1F receptors in monkey vestibular and trigeminal ganglion cells","volume":"7","author":"Usman","year":"2016","journal-title":"Front. Neurol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"408","DOI":"10.1111\/j.1526-4610.1994.hed3407408.x","article-title":"Serotonin (5-HT) and migraine","volume":"34","author":"Silberstei","year":"1994","journal-title":"Headache J. Head Face Pain"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"46","DOI":"10.1177\/1756285614562419","article-title":"The pharmacological profile and clinical prospects of the oral 5-HT1F receptor agonist lasmiditan in the acute treatment of migraine","volume":"8","author":"Reuter","year":"2015","journal-title":"Ther. Adv. Neurol. Disord."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"1230","DOI":"10.1016\/S0140-6736(01)06347-4","article-title":"Selective seratonin 1F (5-HT1F) receptor agonist LY334370 for acute migraine: A randomised controlled trial","volume":"358","author":"Goldstein","year":"2001","journal-title":"Lancet"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"830","DOI":"10.1016\/j.pain.2012.01.005","article-title":"mRNA expression of 5-hydroxytryptamine 1B, 1D, and 1F receptors and their role in controlling the release of calcitonin gene\u2013related peptide in the rat trigeminovascular system","volume":"153","author":"Amrutkar","year":"2012","journal-title":"Pain"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"3281","DOI":"10.1016\/j.celrep.2016.11.072","article-title":"Human beta cells produce and release serotonin to inhibit glucagon secretion from alpha cells","volume":"17","author":"Molina","year":"2016","journal-title":"Cell Rep."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"150","DOI":"10.3382\/ps.0610150","article-title":"Feeding and drinking response of young chicks to injections of serotonin into the lateral ventricle of the brain","volume":"61","author":"Denbow","year":"1982","journal-title":"Poult. Sci."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1016\/0378-4320(78)90011-8","article-title":"Inhibitory effect of serotonin on ovulation in the domestic fowl, Gallus domesticus","volume":"1","author":"Soliman","year":"1978","journal-title":"Anim. Reprod. Sci."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"455","DOI":"10.1530\/acta.0.1050455","article-title":"Serotonin and acetylcholine affect the release of prolactin and growth hormone from pituitary glands of domestic fowl in vitro in the presence of hypothalamic tissue","volume":"105","author":"Hall","year":"1984","journal-title":"Eur. J. Endocrinol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"612","DOI":"10.3382\/ps.2007-00389","article-title":"Serotonergic mediation of aggression in high and low aggressive chicken strains","volume":"87","author":"Dennis","year":"2008","journal-title":"Poult. Sci."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"773","DOI":"10.1111\/age.12050","article-title":"Investigating gene expression differences in two chicken groups with variable propensity to feather pecking","volume":"44","author":"Wysocki","year":"2013","journal-title":"Anim. Genet."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"394","DOI":"10.1017\/S0967199413000683","article-title":"Serotonin receptors are selectively expressed in the avian germ cells and early embryos","volume":"23","author":"Kuwana","year":"2015","journal-title":"Zygote"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"155","DOI":"10.1530\/JOE-17-0131","article-title":"The interaction of MC3R and MC4R with MRAP2, ACTH, \u03b1-MSH and AgRP in chickens","volume":"234","author":"Zhang","year":"2017","journal-title":"J. Endocrinol."},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"Liu, T., Deng, Y., Zhang, Z., Cao, B., Li, J., Sun, C., Hu, Z., Zhang, J., Li, J., and Wang, Y. (2020). Melanocortin Receptor 4 (MC4R) Signaling System in Nile Tilapia. Int. J. Mol. Sci., 21.","DOI":"10.3390\/ijms21197036"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"91","DOI":"10.1016\/j.ygcen.2019.05.013","article-title":"Arginine vasotocin (AVT)\/mesotocin (MT) receptors in chickens: Evidence for the possible involvement of AVT-AVPR1 signaling in the regulation of oviposition and pituitary prolactin expression","volume":"281","author":"Wu","year":"2019","journal-title":"Gen. Comp. Endocrinol."},{"key":"ref_30","first-page":"95","article-title":"BioEdit: A user-friendly biological sequence alignment editor and analysis program for Windows 95\/98\/NT","volume":"41","author":"Hall","year":"1999","journal-title":"Nucleic Acids Symp Ser."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"1870","DOI":"10.1093\/molbev\/msw054","article-title":"MEGA7: Molecular evolutionary genetics analysis version 7.0 for bigger datasets","volume":"33","author":"Kumar","year":"2016","journal-title":"Mol. Biol. Evol."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"408","DOI":"10.1016\/j.bbrc.2018.04.229","article-title":"The orphan G protein-coupled receptor 25 (GPR25) is activated by Apelin and Apela in non-mammalian vertebrates","volume":"501","author":"Zhang","year":"2018","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"756","DOI":"10.3389\/fendo.2018.00756","article-title":"Characterization of the Apelin\/Elabela Receptors (APLNR) in Chickens, Turtles, and Zebrafish: Identification of a Novel Apelin-Specific Receptor in Teleosts","volume":"9","author":"Zhang","year":"2018","journal-title":"Front. Endocrinol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"46","DOI":"10.1016\/j.ygcen.2016.09.005","article-title":"Molecular characterization of neuropeptide Y (NPY) receptors (Y1, Y4 and Y6) and investigation of the tissue expression of their ligands (NPY, PYY and PP) in chickens","volume":"240","author":"Gao","year":"2017","journal-title":"Gen. Comp. Endocrinol."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"149","DOI":"10.1016\/j.mce.2013.03.001","article-title":"Molecular characterization of prolactin receptor (cPRLR) gene in chickens: Gene structure, tissue expression, promoter analysis, and its interaction with chicken prolactin (cPRL) and prolactin-like protein (cPRL-L)","volume":"370","author":"Bu","year":"2013","journal-title":"Mol. Cell. Endocrinol."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"3562","DOI":"10.1210\/en.2016-1141","article-title":"Characterization of neuropeptide B (NPB), neuropeptide W (NPW), and their receptors in chickens: Evidence for NPW being a novel inhibitor of pituitary GH and prolactin secretion","volume":"157","author":"Bu","year":"2016","journal-title":"Endocrinology"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"D816","DOI":"10.1093\/nar\/gkx1003","article-title":"Genomicus 2018: Karyotype evolutionary trees and on-the-fly synteny computing","volume":"46","author":"Nguyen","year":"2018","journal-title":"Nucleic Acids Res."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"264","DOI":"10.1007\/s002130050292","article-title":"CP-94,253: A selective serotonin1B (5-HT1B) agonist that promotes satiety","volume":"131","author":"Lee","year":"1997","journal-title":"Psychopharmacology"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"46","DOI":"10.1016\/j.ejphar.2005.04.025","article-title":"Antidepressant-like effect of the selective 5-HT1B receptor agonist CP 94253: A possible mechanism of action","volume":"516","author":"Stachowicz","year":"2005","journal-title":"Eur. J. Pharmacol."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"644","DOI":"10.1093\/ijnp\/pyx025","article-title":"The selective 5-HT1B receptor agonist, CP 94,253, atten-uates methamphetamine self-administration in male rats","volume":"20","author":"Garcia","year":"2017","journal-title":"J. Int. Neuropsychopharmacol."},{"key":"ref_41","unstructured":"Lightowler, S., Stean, T., Upton, N., Vimal, M., Kennett, G., Porter, R., and Brown, A. (1998). Effect of BRL 54443 (3-(-1-methylpiperidin-4-yl)-1H-indol-5-ol), a 5-ht1E\/1F receptor agonist, on general behaviour and maximal electroshock seizure threshold in the rat. Br. J. Pharmacol. Proc. Suppl., 237P."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"860","DOI":"10.1124\/jpet.111.179606","article-title":"Toward selective drug development for the human 5-hydroxytryptamine 1E receptor: A comparison of 5-hydroxytryptamine 1E and 1F receptor structure-affinity relationships","volume":"337","author":"Klein","year":"2011","journal-title":"J. Pharmacol. Exp. Ther."},{"key":"ref_43","unstructured":"Brown, A., Avenell, K., Young, T., Ho, M., Porter, R., Vimal, M., and Middlemiss, D. (1998). BRL 54443, a potent agonist with selectivity for human cloned 5-ht1E and 5-ht1F receptors. Br. J. Pharmacol. Proc. Suppl., 233P."},{"key":"ref_44","first-page":"935","article-title":"5-Hydroxytryptamine1F receptors do not participate in vasoconstriction: Lack of vasoconstriction to LY344864, a selective serotonin1F receptor agonist in rabbit saphenous vein","volume":"290","author":"Cohen","year":"1999","journal-title":"J. Pharmacol. Exp."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1016\/j.pbb.2018.08.001","article-title":"Intracerebroventricular microinjection of the 5-HT1F receptor agonist LY 344864 inhibits methamphetamine conditioned place preference reinstatement in rats","volume":"173","author":"Shahidi","year":"2018","journal-title":"Pharmacol. Biochem. Behav."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"291","DOI":"10.1007\/s13311-018-0615-6","article-title":"Targeted 5-HT 1F therapies for migraine","volume":"15","year":"2018","journal-title":"Neurotherapeutics"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"275","DOI":"10.1016\/0028-3908(94)90059-0","article-title":"Molecular biology of 5-HT receptors","volume":"33","author":"Boess","year":"1994","journal-title":"Neuropharmacology"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"498","DOI":"10.1038\/nature12111","article-title":"The zebrafish reference genome sequence and its relationship to the human genome","volume":"496","author":"Howe","year":"2013","journal-title":"Nature"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"254","DOI":"10.1038\/335254a0","article-title":"Serotonin stimulates DNA synthesis in fibroblasts acting through 5\u2013HT 1B receptors coupled to a G i-protein","volume":"335","author":"Seuwen","year":"1988","journal-title":"Nature"},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"285","DOI":"10.1007\/BF00168512","article-title":"5-Hydroxytryptamine 5-HT 1B and 5-HT 1D receptors mediating inhibition of adenylate cyclase activity","volume":"340","author":"Schoeffter","year":"1989","journal-title":"Naunyn-Schmiedeberg\u2032s Arch. Pharmacol."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"934","DOI":"10.1046\/j.1471-4159.2000.0750934.x","article-title":"Activation of Extracellular Signal-Regulated Kinase (ERK) and Akt by Human Serotonin 5-HT1B Receptors in Transfected BE (2)-C Neuroblastoma Cells Is Inhibited by RGS4","volume":"75","author":"Lione","year":"2000","journal-title":"J. Neurochem."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"162","DOI":"10.1046\/j.1471-4159.1999.0730162.x","article-title":"Differential coupling of serotonin 5-HT1A and 5-HT1B receptors to activation of ERK2 and inhibition of adenylyl cyclase in transfected CHO cells","volume":"73","author":"Mendez","year":"1999","journal-title":"J. Neurochem."},{"key":"ref_53","first-page":"S21","article-title":"The Concise Guide to PHARMACOLOGY 2019\/20: G protein-coupled receptors","volume":"176","author":"Alexander","year":"2019","journal-title":"Br. J. Pharmacol."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"127","DOI":"10.1016\/j.ejphar.2003.11.019","article-title":"Molecular cloning and pharmacological characterization of the guinea pig 5-HT1E receptor","volume":"484","author":"Bai","year":"2004","journal-title":"Eur. J. Pharmacol."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"259","DOI":"10.1016\/j.ejphar.2004.03.054","article-title":"Influence of 5-HT1 receptor agonists on feline stomach relaxation","volume":"492","author":"Janssen","year":"2004","journal-title":"Eur. J. Pharmacol."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"257","DOI":"10.1124\/jpet.114.214700","article-title":"Agonism of the 5-hydroxytryptamine 1F receptor promotes mitochondrial biogenesis and recovery from acute kidney injury","volume":"350","author":"Garrett","year":"2014","journal-title":"J. Pharmacol. Exp. Ther."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"F1468","DOI":"10.1152\/ajprenal.00246.2007","article-title":"Characterization of a putative intrarenal serotonergic system","volume":"293","author":"Xu","year":"2007","journal-title":"Am. J. Physiol. Ren. Physiol."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"453","DOI":"10.1016\/S1043-2760(01)00498-2","article-title":"Receptor signaling and structure: Insights from serotonin-1 receptors","volume":"12","author":"Albert","year":"2001","journal-title":"Trends Endocrinol. Metab."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"891","DOI":"10.1038\/sj.bjp.0704150","article-title":"Molecular cloning, pharmacological properties and tissue distribution of the porcine 5-HT1B receptor","volume":"133","author":"Bhalla","year":"2001","journal-title":"Br. J. Pharmacol."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"469","DOI":"10.1016\/S0306-4522(97)00302-3","article-title":"Detailed mapping of serotonin 5-HT1B and 5-HT1D receptor messenger RNA and ligand binding sites in guinea-pig brain and trigeminal ganglion: Clues for function","volume":"82","author":"Bonaventure","year":"1997","journal-title":"Neuroscience"},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1002\/syn.20128","article-title":"Regional expression of 5-HT1B receptor mRNA in the human brain","volume":"56","author":"Hurd","year":"2005","journal-title":"Synapse"},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"521","DOI":"10.1002\/cne.21831","article-title":"Comparative analysis of serotonin receptor (HTR1A\/HTR1B families) and transporter (slc6a4a\/b) gene expression in the zebrafish brain","volume":"511","author":"Norton","year":"2008","journal-title":"J. Comp. Neurol."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"884","DOI":"10.1523\/JNEUROSCI.21-03-00884.2001","article-title":"Excessive activation of serotonin (5-HT) 1B receptors disrupts the formation of sensory maps in monoamine oxidase a and 5-ht transporter knock-out mice","volume":"21","author":"Salichon","year":"2001","journal-title":"J. Neurosci."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"21183","DOI":"10.1038\/s41598-020-78247-9","article-title":"The dorsal arcopallium of chicks displays the expression of orthologs of mammalian fear related serotonin receptor subfamily genes","volume":"10","author":"Fujita","year":"2020","journal-title":"Sci. Rep."},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"1290","DOI":"10.1111\/j.1476-5381.2012.01868.x","article-title":"Distribution of 5-ht1E receptors in the mammalian brain and cerebral vasculature: An immunohistochemical and pharmacological study","volume":"166","author":"Klein","year":"2012","journal-title":"Br. J. Pharmacol."},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"367","DOI":"10.1016\/0028-3908(94)90067-1","article-title":"Localization of 5-HT1B, 5-HT1D\u03b1, 5-HT1E and 5-HT1F receptor messenger RNA in rodent and primate brain","volume":"33","author":"Bruinvels","year":"1994","journal-title":"Neuropharmacology"},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1016\/S0021-5198(19)32437-0","article-title":"The effects of the serotonin1A receptor agonist buspirone on the blood glucose and pancreatic hormones in rats","volume":"60","author":"Sugimoto","year":"1992","journal-title":"Jpn. J. Pharmacol."},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"47","DOI":"10.1016\/0167-0115(96)00034-1","article-title":"The oxytocin receptor antagonist 1-deamino-2-D-Tyr-(OEt)-4-Thr-8-Orn-oxytocin inhibits effects of the 5-HT1A receptor agonist 8-OH-DPAT on plasma levels of insulin, cholecystokinin and somatostatin","volume":"63","author":"Smedh","year":"1996","journal-title":"Regul. Pept."},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"1603","DOI":"10.1016\/j.lfs.2005.07.027","article-title":"Hypothalamic 5-HT functional regulation through 5-HT1A and 5-HT2C receptors during pancreatic regeneration","volume":"78","author":"Khan","year":"2006","journal-title":"Life Sci."},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1016\/S0014-2999(01)01605-3","article-title":"Molecular cloning and expression of the porcine trigeminal ganglion cDNA encoding a 5-ht1F receptor","volume":"436","author":"Bhalla","year":"2002","journal-title":"Eur. J. Pharmacol."},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"271","DOI":"10.1016\/0014-2999(95)00748-2","article-title":"[3H] Sumatriptan binding sites in human brain: Regional-dependent labelling of 5-HT1D and 5-HT1F receptors","volume":"295","author":"Pascual","year":"1996","journal-title":"Eur. J. Pharmacol."},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"176","DOI":"10.1016\/j.nurt.2010.03.003","article-title":"5-HT 1F receptor agonists: A new treatment option for migraine attacks?","volume":"7","author":"Neeb","year":"2010","journal-title":"Neurotherapeutics"},{"key":"ref_73","doi-asserted-by":"crossref","unstructured":"Ouyang, Q., Hu, S., Wang, G., Hu, J., Zhang, J., Li, L., Hu, B., He, H., Liu, H., and Xia, L. (2020). Comparative Transcriptome Analysis Suggests Key Roles for 5-Hydroxytryptamlne Receptors in Control of Goose Egg Production. Genes, 11.","DOI":"10.3390\/genes11040455"},{"key":"ref_74","unstructured":"Sturkie, P.D. (2012). Avian Physiology, Springer Science & Business Media."},{"key":"ref_75","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1038\/s41598-020-62886-z","article-title":"Transcriptome analysis reveals differentially expressed genes associated with high rates of egg production in chicken hypothalamic-pituitary-ovarian axis","volume":"10","author":"Mishra","year":"2020","journal-title":"Sci. Rep."},{"key":"ref_76","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1016\/j.gene.2018.01.087","article-title":"Dopamine D2-like receptors (DRD2 and DRD4) in chickens: Tissue distribution, functional analysis, and their involvement in dopamine inhibition of pituitary prolactin expression","volume":"651","author":"Lv","year":"2018","journal-title":"Gene"},{"key":"ref_77","doi-asserted-by":"crossref","first-page":"1384","DOI":"10.1210\/en.2006-1198","article-title":"Estradiol induces expression of 5-hydroxytryptamine (5-HT) 4, 5-HT5, and 5-HT6 receptor messenger ribonucleic acid in rat anterior pituitary cell aggregates and allows prolactin release via the 5-HT4 receptor","volume":"148","author":"Papageorgiou","year":"2007","journal-title":"Endocrinology"},{"key":"ref_78","doi-asserted-by":"crossref","first-page":"159","DOI":"10.1159\/000126836","article-title":"Differential Sites of Action of 8OH-DPAT, a 5HT1A Agonist, on ACTH and PRL Secretion in the Rat","volume":"61","author":"Pajot","year":"1995","journal-title":"Neuroendocrinology"},{"key":"ref_79","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1159\/000054297","article-title":"Role of vasoactive intestinal peptide and 5-ht2 receptor subtype in serotonin stimulation of basal and thyrotropin-releasing-hormone-induced prolactin release in vitro from rat pituitary cells","volume":"67","author":"Apfelbaum","year":"1998","journal-title":"Neuroendocrinology"},{"key":"ref_80","doi-asserted-by":"crossref","first-page":"336","DOI":"10.1159\/000126134","article-title":"Involvement of 5-HT1, 5-HT2, and 5-HT3 receptors in the mediation of the prolactin response to serotonin and 5-hydroxytryptophan","volume":"55","author":"Knigge","year":"1992","journal-title":"Neuroendocrinology"}],"container-title":["Genes"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2073-4425\/12\/6\/891\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T06:12:34Z","timestamp":1760163154000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2073-4425\/12\/6\/891"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,6,9]]},"references-count":80,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2021,6]]}},"alternative-id":["genes12060891"],"URL":"https:\/\/doi.org\/10.3390\/genes12060891","relation":{},"ISSN":["2073-4425"],"issn-type":[{"value":"2073-4425","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,6,9]]}}}