{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,20]],"date-time":"2026-05-20T16:02:37Z","timestamp":1779292957398,"version":"3.51.4"},"reference-count":72,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2021,9,9]],"date-time":"2021-09-09T00:00:00Z","timestamp":1631145600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004837","name":"Ministerio de Ciencia e Innovaci\u00f3n","doi-asserted-by":"publisher","award":["RTI2018-095997-B-I00"],"award-info":[{"award-number":["RTI2018-095997-B-I00"]}],"id":[{"id":"10.13039\/501100004837","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100010556","name":"Conseller\u00eda de Econom\u00eda, Emprego e Industria, Xunta de Galicia","doi-asserted-by":"publisher","award":["IN607B 2019\/01"],"award-info":[{"award-number":["IN607B 2019\/01"]}],"id":[{"id":"10.13039\/501100010556","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100010556","name":"Conseller\u00eda de Econom\u00eda, Emprego e Industria, Xunta de Galicia","doi-asserted-by":"publisher","award":["IN606B-2018\/010"],"award-info":[{"award-number":["IN606B-2018\/010"]}],"id":[{"id":"10.13039\/501100010556","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100013276","name":"Interreg","doi-asserted-by":"publisher","award":["0474_BLUEBIOLAB"],"award-info":[{"award-number":["0474_BLUEBIOLAB"]}],"id":[{"id":"10.13039\/100013276","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Genes"],"abstract":"<jats:p>The Mediterranean mussel is one of the most economically relevant bivalve mollusk species in Europe and China. The absence of massive mortalities and their resistance to pathogens affecting other cultured bivalves has been under study in recent years. The transcriptome response of this species to different immune stimuli has been extensively studied, and even the complexity of its genome, which has recently been sequenced, has been suggested as one of the factors contributing to this resistance. However, studies concerning the non-coding RNA profiles remain practically unexplored\u2014especially those corresponding to the lncRNAs. To the best of our knowledge, this is the second characterization and study of lncRNAs in this bivalve species. In this work, we identified the potential repertoire of lncRNAs expressed in mussel hemocytes, and using RNA-Seq we analyzed the lncRNA profile of mussel hemocytes stimulated in vitro with three different immune stimuli: LPS, poly I:C, and \u03b2-glucans. Compared to unstimulated hemocytes, LPS induced the highest modulation of lncRNAs, whereas poly I:C and \u03b2-glucans induced a similar discrete response. Based on the potential cis-regulatory activity of the lncRNAs, we identified the neighboring protein-coding genes of the regulated lncRNAs to estimate\u2014at least partially\u2014the processes in which they are implicated. After applying correlation analyses, it seems that\u2014especially for LPS\u2014the lncRNAs could participate in the regulation of gene expression, and substantially contribute to the immune response.<\/jats:p>","DOI":"10.3390\/genes12091393","type":"journal-article","created":{"date-parts":[[2021,9,9]],"date-time":"2021-09-09T12:20:37Z","timestamp":1631190037000},"page":"1393","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["Differential Expression of Long Non-Coding RNA (lncRNA) in Mediterranean Mussel (Mytilus galloprovincialis) Hemocytes under Immune Stimuli"],"prefix":"10.3390","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8734-3334","authenticated-orcid":false,"given":"Patricia","family":"Pereiro","sequence":"first","affiliation":[{"name":"Instituto de Investigaciones Marinas (IIM), Consejo Superior de Investigaciones Cient\u00edficas (CSIC), Eduardo Cabello, 6, 36208 Vigo, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rebeca","family":"Moreira","sequence":"additional","affiliation":[{"name":"Instituto de Investigaciones Marinas (IIM), Consejo Superior de Investigaciones Cient\u00edficas (CSIC), Eduardo Cabello, 6, 36208 Vigo, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Beatriz","family":"Novoa","sequence":"additional","affiliation":[{"name":"Instituto de Investigaciones Marinas (IIM), Consejo Superior de Investigaciones Cient\u00edficas (CSIC), Eduardo Cabello, 6, 36208 Vigo, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0617-0030","authenticated-orcid":false,"given":"Antonio","family":"Figueras","sequence":"additional","affiliation":[{"name":"Instituto de Investigaciones Marinas (IIM), Consejo Superior de Investigaciones Cient\u00edficas (CSIC), Eduardo Cabello, 6, 36208 Vigo, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2021,9,9]]},"reference":[{"key":"ref_1","unstructured":"FAO (2021, September 08). Food and Agriculture Organization (2004\u20132020). FAO Fisheries Division; Cultured Aquatic Species Information Programme: Mytilus galloprovincialis. Available online: http:\/\/www.fao.org\/fishery\/culturedspecies\/Mytilus_galloprovincialis\/en."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"291","DOI":"10.1016\/0166-445X(91)90054-D","article-title":"Evidence for a multixenobiotic resistance mechanism in the mussel Mytilus galloprovincialis","volume":"19","author":"Kurelec","year":"1991","journal-title":"Aquat. Toxicol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"133","DOI":"10.1080\/10641260802325518","article-title":"Study of diseases and the immune system of bivalves using molecular biology and genomics","volume":"16","author":"Gestal","year":"2008","journal-title":"Rev. Fish. Sci. Aquac."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1016\/j.fsi.2014.05.023","article-title":"Mortality occurrence and pathogen detection in Crassostrea gigas and Mytilus galloprovincialis close-growing in shallow waters (Goro lagoon, Italy)","volume":"41","author":"Domeneghetti","year":"2014","journal-title":"Fish. Shellfish Immunol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"149","DOI":"10.3354\/dao02701","article-title":"Occurrence, seasonality and infectivity of Vibrio strains in natural populations of mussels Mytilus galloprovincialis","volume":"108","author":"Romero","year":"2014","journal-title":"Dis. Aquat. Organ."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"85","DOI":"10.1016\/S0014-5793(00)02192-X","article-title":"Original involvement of antimicrobial peptides in mussel innate immunity","volume":"486","author":"Mitta","year":"2000","journal-title":"FEBS Lett."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1016\/j.dci.2007.05.008","article-title":"High sequence variability of myticin transcripts in hemocytes of immune-stimulated mussels suggests ancient host-pathogen interactions","volume":"32","author":"Pallavicini","year":"2008","journal-title":"Dev. Comp. Immunol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"162","DOI":"10.1016\/j.dci.2008.08.005","article-title":"Evidence of high individual diversity on myticin C in mussel (Mytilus galloprovincialis)","volume":"33","author":"Costa","year":"2009","journal-title":"Dev. Comp. Immunol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"7692","DOI":"10.1128\/JVI.00591-16","article-title":"Antiviral activity of myticin C peptide from mussel: An ancient defense against herpesviruses","volume":"90","author":"Novoa","year":"2016","journal-title":"J. Virol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"635","DOI":"10.1016\/j.dci.2011.01.018","article-title":"The C1q domain containing proteins of the Mediterranean mussel Mytilus galloprovincialis: A widespread and diverse family of immune-related molecules","volume":"35","author":"Gerdol","year":"2011","journal-title":"Dev. Comp. Immunol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"334","DOI":"10.1016\/j.dci.2010.10.007","article-title":"Individual sequence variability and functional activities of fibrinogen-related proteins (FREPs) in the Mediterranean mussel (Mytilus galloprovincialis) suggest ancient and complex immune recognition models in invertebrates","volume":"35","author":"Romero","year":"2011","journal-title":"Dev. Comp. Immunol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"158","DOI":"10.1016\/j.dci.2013.02.006","article-title":"Toll-like receptors and MyD88 adaptors in Mytilus: Complete cds and gene expression levels","volume":"40","author":"Toubiana","year":"2013","journal-title":"Dev. Comp. Immunol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"255","DOI":"10.1016\/j.dci.2015.05.001","article-title":"IL-17 signaling components in bivalves: Comparative sequence analysis and involvement in the immune responses","volume":"52","author":"Rosani","year":"2015","journal-title":"Dev. Comp. Immunol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1016\/j.fsi.2015.02.013","article-title":"An updated molecular basis for mussel immunity","volume":"46","author":"Gerdol","year":"2015","journal-title":"Fish Shellfish. Immunol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1016\/S0378-1119(02)01149-6","article-title":"Immune gene discovery by expressed sequence tags generated from hemocytes of the bacteria-challenged oyster, Crassostrea gigas","volume":"303","author":"Gueguen","year":"2003","journal-title":"Gene"},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"De Lorgeril, J., Zenagui, R., Rosa, R.D., Piquemal, D., and Bach\u00e8re, E. (2011). Whole transcriptome profiling of successful immune response to Vibrio infections in the oyster Crassostrea gigas by digital gene expression analysis. PLoS ONE, 6.","DOI":"10.1371\/journal.pone.0023142"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"382","DOI":"10.1016\/j.fsi.2013.04.051","article-title":"Poly I:C induces a protective antiviral immune response in the Pacific oyster (Crassostrea gigas) against subsequent challenge with Ostreid herpesvirus (OsHV-1 \u03bcvar)","volume":"35","author":"Green","year":"2013","journal-title":"Fish. Shellfish Immunol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1080\/13235818.2014.919696","article-title":"Comparative analysis of oyster (Crassostrea gigas) immune responses under challenge by different Vibrio strains and conditions","volume":"35","author":"Meng","year":"2015","journal-title":"Molluscan Res."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"72","DOI":"10.1186\/s13567-016-0356-7","article-title":"Distinct immune responses of juvenile and adult oysters (Crassostrea gigas) to viral and bacterial infections","volume":"47","author":"Green","year":"2016","journal-title":"Vet. Res."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"e02777-19","DOI":"10.1128\/mBio.02777-19","article-title":"A sustained immune response supports long-term antiviral immune priming in in the Pacific Oyster, Crassostrea Gigas.","volume":"11","author":"Lafont","year":"2020","journal-title":"Mbio"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"515","DOI":"10.1016\/j.fsi.2009.02.001","article-title":"Functional and molecular immune response of Mediterranean mussel (Mytilus galloprovincialis) haemocytes against pathogen-associated molecular patterns and bacteria","volume":"26","author":"Costa","year":"2009","journal-title":"Fish. Shellfish Immunol."},{"key":"ref_22","doi-asserted-by":"crossref","unstructured":"Venier, P., Varotto, L., Rosani, U., Millino, C., Celegato, B., Bernante, F., Lanfranchi, G., Novoa, B., Roch, P., and Figueras, A. (2011). Insights into the innate immunity of the Mediterranean mussel Mytilus Galloprovincialis..  BMC Genom., 12.","DOI":"10.1186\/1471-2164-12-69"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"438","DOI":"10.1016\/j.fsi.2013.04.044","article-title":"Immune responses during the larval stages of Mytilus galloprovincialis: Metamorphosis alters immunocompetence, body shape and behavior","volume":"35","author":"Balseiro","year":"2013","journal-title":"Fish. Shellfish Immunol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"3569","DOI":"10.1038\/s41598-019-39870-3","article-title":"High individual variability in the transcriptomic response of Mediterranean mussels to Vibrio reveals the involvement of myticins in tissue injury","volume":"9","author":"Moreira","year":"2019","journal-title":"Sci. Rep."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"1894","DOI":"10.3389\/fimmu.2019.01894","article-title":"Immune Tolerance in Mytilus galloprovincialis hemocytes after repeated contact with Vibrio splendidus","volume":"10","author":"Moreira","year":"2019","journal-title":"Front. Immunol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"606102","DOI":"10.3389\/fimmu.2020.606102","article-title":"Stimulation of Mytilus galloprovincialis hemocytes with different immune challenges induces differential transcriptomic, miRNomic, and functional responses","volume":"11","author":"Moreira","year":"2020","journal-title":"Front. Immunol."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"629","DOI":"10.1016\/j.cell.2009.02.006","article-title":"Evolution and functions of long noncoding RNAs","volume":"136","author":"Ponting","year":"2009","journal-title":"Cell"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"47","DOI":"10.1038\/nrg.2015.10","article-title":"Unique features of long non-coding RNA biogenesis and function","volume":"17","author":"Quinn","year":"2016","journal-title":"Nat. Rev. Genet."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"485","DOI":"10.1007\/s10126-016-9711-y","article-title":"Long non-coding RNAs (lncRNAs) of sea cucumber: Large-scale prediction, expression profiling, non-coding network construction, and lncRNA-microRNA-Gene interaction analysis of lncRNAs in Apostichopus japonicus and Holothuria glaberrima during LPS challenge and radial organ complex regeneration","volume":"18","author":"Mu","year":"2016","journal-title":"Mar. Biotechnol."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"233","DOI":"10.1016\/j.fsi.2018.07.032","article-title":"Differential lncRNA expression profiles reveal the potential roles of lncRNAs in antiviral immune response of Crassostrea gigas","volume":"81","author":"Sun","year":"2018","journal-title":"Fish. Shellfish Immunol."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"1425","DOI":"10.1016\/j.ygeno.2019.08.012","article-title":"Re-thinking adaptive immunity in the beetles: Evolutionary and functional trajectories of lncRNAs","volume":"112","author":"Ali","year":"2020","journal-title":"Genomics"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"7738","DOI":"10.4049\/jimmunol.0900603","article-title":"Genome-wide identification of long noncoding RNAs in CD8+ T cells","volume":"182","author":"Pang","year":"2009","journal-title":"J. Immunol."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"e00206","DOI":"10.1128\/mBio.00206-10","article-title":"Unique signatures of long noncoding RNA expression in response to virus infection and altered innate immune signaling","volume":"1","author":"Peng","year":"2010","journal-title":"Mbio"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"14174","DOI":"10.1038\/s41598-019-50766-0","article-title":"Comparative modulation of lncRNAs in wild-type and rag1-heterozygous mutant zebrafish exposed to immune challenge with spring viraemia of carp virus (SVCV)","volume":"9","author":"Pereiro","year":"2019","journal-title":"Sci. Rep."},{"key":"ref_35","doi-asserted-by":"crossref","unstructured":"Pereiro, P., Lama, R., Moreira, R., Valenzuela-Mu\u00f1oz, V., Gallardo-Esc\u00e1rate, C., Novoa, B., and Figueras, A. (2020). Potential involvement of lncRNAs in the modulation of the transcriptome response to nodavirus challenge in European sea bass (Dicentrarchus labrax L.). Biology, 9.","DOI":"10.3390\/biology9070165"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1124\/mol.110.064055","article-title":"Inhibition of human T cell proliferation by mammalian target of rapamycin (mTOR) antagonists requires noncoding RNA growth-arrest-specific transcript 5 (GAS5)","volume":"78","author":"Hasan","year":"2010","journal-title":"Mol. Pharmacol."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"e00762","DOI":"10.7554\/eLife.00762","article-title":"A mammalian pseudogene lncRNA at the interface of inflammation and anti-inflammatory therapeutics","volume":"2","author":"Rapicavoli","year":"2013","journal-title":"eLife"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"315","DOI":"10.1016\/j.dci.2014.01.015","article-title":"Knockdown of PU.1 mRNA and AS lncRNA regulates expression of immune-related genes in zebrafish Danio rerio","volume":"44","author":"Wei","year":"2014","journal-title":"Dev. Comp. Immunol."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"108","DOI":"10.1016\/j.fsi.2018.07.020","article-title":"Regulation of IL-17 by lncRNA of IRF-2 in the pearl oyster","volume":"81","author":"Huang","year":"2018","journal-title":"Fish. Shellfish Immunol."},{"key":"ref_40","doi-asserted-by":"crossref","unstructured":"Valanne, S., Salminen, T.S., J\u00e4rvel\u00e4-St\u00f6lting, M., Vesala, L., and R\u00e4met, M. (2019). Immune-inducible non-coding RNA molecule lincRNA-IBIN connects immunity and metabolism in Drosophila melanogaster. PLoS Pathog., 15.","DOI":"10.1371\/journal.ppat.1008088"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"1495","DOI":"10.1038\/s41467-019-09482-6","article-title":"The lncRNA Neat1 promotes activation of inflammasomes in macrophages","volume":"10","author":"Zhang","year":"2019","journal-title":"Nat. Commun."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"145300","DOI":"10.1016\/j.gene.2020.145300","article-title":"The functional roles of the non-coding RNAs in molluscs","volume":"768","author":"Hongkuan","year":"2021","journal-title":"Gene"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"615580","DOI":"10.3389\/fimmu.2020.615580","article-title":"Transcriptomic response of mussel gills after a Vibrio splendidus infection demonstrates their role in the immune response","volume":"11","author":"Saco","year":"2020","journal-title":"Front. Immunol."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"193","DOI":"10.1016\/j.dci.2017.03.024","article-title":"In the shadow: The emerging role of long non-coding RNAs in the immune response of Atlantic salmon","volume":"73","year":"2017","journal-title":"Dev. Comp. Immunol."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"e74","DOI":"10.1093\/nar\/gkt006","article-title":"CPAT: Coding-Potential Assessment Tool using an alignment-free logistic regression model","volume":"41","author":"Wang","year":"2013","journal-title":"Nucleic Acids Res."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"275","DOI":"10.1186\/s13059-020-02180-3","article-title":"Massive gene presence-absence variation shapes an open pan-genome in the Mediterranean mussel","volume":"21","author":"Gerdol","year":"2020","journal-title":"Genome Biol."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"W3","DOI":"10.1093\/nar\/gkw343","article-title":"The Galaxy platform for accessible, reproducible and collaborative biomedical analyses: 2016 update","volume":"44","author":"Afgan","year":"2016","journal-title":"Nucleic Acids Res."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"W147","DOI":"10.1093\/nar\/gkw419","article-title":"Heatmapper: Web-enabled heat mapping for all","volume":"44","author":"Babicki","year":"2016","journal-title":"Nucleic Acids Res."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"3674","DOI":"10.1093\/bioinformatics\/bti610","article-title":"Blast2GO: A universal tool for annotation, visualization and analysis in functional genomics research","volume":"21","author":"Conesa","year":"2005","journal-title":"Bioinformatics"},{"key":"ref_50","first-page":"365","article-title":"Primer3 on the WWW for general users and for biologist programmers","volume":"132","author":"Rozen","year":"2000","journal-title":"Methods Mol. Biol."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"e45","DOI":"10.1093\/nar\/29.9.e45","article-title":"A new mathematical model for relative quantification in real-time RT-PCR","volume":"29","author":"Pfaffl","year":"2001","journal-title":"Nucleic Acids Res."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"147","DOI":"10.1051\/alr\/2014015","article-title":"Evaluation of reference genes of Mytilus galloprovincialis and Ruditapes philippinarum infected with three bacteria strains for gene expression analysis","volume":"27","author":"Moreira","year":"2014","journal-title":"Aquat. Living Resour."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1016\/j.fsi.2014.01.002","article-title":"Bioinformatics analysis of hemocyte miRNAs of scallop Chlamys farreri against acute viral necrobiotic virus (AVNV)","volume":"37","author":"Chen","year":"2014","journal-title":"Fish. Shellfish Immunol."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"54","DOI":"10.1007\/s10126-013-9528-x","article-title":"Identification and characterization of MicroRNAs in pearl oyster Pinctada martensii by Solexa deep sequencing","volume":"16","author":"Jiao","year":"2014","journal-title":"Mar. Biotechnol."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"40","DOI":"10.1016\/j.meegid.2014.06.026","article-title":"Role of microRNAs in the immunity process of the flat oyster Ostrea edulis against bonamiosis","volume":"27","author":"Villalba","year":"2014","journal-title":"Infect. Genet. Evol"},{"key":"ref_56","doi-asserted-by":"crossref","unstructured":"Zhou, Z., Wang, L., Song, L., Liu, R., Zhang, H., Huang, M., and Chen, H. (2014). The identification and characteristics of immune-related microRNAs in haemocytes of oyster Crassostrea gigas. PLoS ONE, 9.","DOI":"10.1371\/journal.pone.0088397"},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1016\/j.margen.2015.07.004","article-title":"Draft genome assemblies and predicted microRNA complements of the intertidal lophotrochozoans Patella vulgata (Mollusca, Patellogastropoda) and Spirobranchus (Pomatoceros) lamarcki (Annelida, Serpulida)","volume":"24","author":"Kenny","year":"2015","journal-title":"Mar. Genom."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"372","DOI":"10.1016\/j.sjbs.2015.04.001","article-title":"Computational prediction of candidate miRNAs and their potential functions in biomineralization in pearl oyster Pinctada martensii","volume":"23","author":"Zheng","year":"2016","journal-title":"Saudi J. Biol. Sci."},{"key":"ref_59","doi-asserted-by":"crossref","unstructured":"Huang, J., Luo, X., Huang, M., Liu, G., You, W., and Ke, C. (2018). Identification and characteristics of muscle growth-related microRNA in the Pacific abalone, Haliotis discus hannai. BMC Genom., 19.","DOI":"10.1186\/s12864-018-5347-9"},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"108697","DOI":"10.1016\/j.cbpc.2019.108697","article-title":"Profiling of microRNAs and mRNAs in marine mussel Mytilus galloprovincialis","volume":"230","author":"Yu","year":"2020","journal-title":"Comp. Biochem. Physiol. C Toxicol. Pharmacol."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"20796","DOI":"10.1038\/srep20796","article-title":"Genome-wide identification and characterization of long intergenic noncoding RNAs and their potential association with larval development in the Pacific oyster","volume":"6","author":"Yu","year":"2016","journal-title":"Sci. Rep."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1016\/j.margen.2017.01.002","article-title":"Long non-coding RNAs are associated with spatiotemporal gene expression profiles in the marine gastropod Tegula atra","volume":"33","author":"Tapia","year":"2017","journal-title":"Mar. Genom."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"16839","DOI":"10.1038\/s41598-018-35202-z","article-title":"Expression profiling of lncRNAs and mRNAs reveals regulation of muscle growth in the Pacific abalone, Haliotis discus hannai","volume":"8","author":"Huang","year":"2018","journal-title":"Sci. Rep."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"1436","DOI":"10.1038\/s41598-018-19950-6","article-title":"Transcriptional profiling of long non-coding RNAs in mantle of Crassostrea gigas and their association with shell pigmentation","volume":"8","author":"Feng","year":"2018","journal-title":"Sci. Rep."},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"209","DOI":"10.1016\/j.fsi.2019.05.037","article-title":"The global effects of PmRunt co-located and co-expressed with a lincRNA lncRunt in pearl oyster Pinctada fucata martensii","volume":"91","author":"Zheng","year":"2019","journal-title":"Fish. Shellfish Immunol."},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"330","DOI":"10.1016\/j.fsi.2019.12.015","article-title":"LncMSEN1, a mantle-specific LncRNA participating in nacre formation and response to polyI:C stimulation in pearl oyster Pinctada fucata martensii","volume":"96","author":"Zheng","year":"2020","journal-title":"Fish. Shellfish Immunol."},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"7120","DOI":"10.18632\/oncotarget.6859","article-title":"Identifying and functionally characterizing tissue-specific and ubiquitously expressed human lncRNAs","volume":"7","author":"Jiang","year":"2016","journal-title":"Oncotarget"},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"102","DOI":"10.1038\/s41576-019-0184-5","article-title":"Regulation of gene expression by cis-acting long non-coding RNAs","volume":"21","author":"Gil","year":"2020","journal-title":"Nat. Rev. Genet."},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"1110","DOI":"10.1016\/j.celrep.2015.04.023","article-title":"Principles of long noncoding RNA evolution derived from direct comparison of transcriptomes in 17 species","volume":"11","author":"Hezroni","year":"2015","journal-title":"Cell Rep."},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"3882","DOI":"10.1242\/dev.140962","article-title":"The functions of long noncoding RNAs in development and stem cells","volume":"143","author":"Perry","year":"2016","journal-title":"Development"},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.jnutbio.2015.11.002","article-title":"Role of lipids in the metabolism and activation of immune cells","volume":"34","author":"Hubler","year":"2016","journal-title":"J. Nutr. Biochem."},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"226","DOI":"10.1016\/S0014-5793(99)01268-5","article-title":"A novel human UDP-N-acetyl-D-galactosamine: Polypeptide N-acetylgalactosaminyltransferase, GalNAc-T7, with specificity for partial GalNAc-glycosylated acceptor substrates","volume":"460","author":"Bennett","year":"1999","journal-title":"FEBS Lett."}],"container-title":["Genes"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2073-4425\/12\/9\/1393\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T06:59:33Z","timestamp":1760165973000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2073-4425\/12\/9\/1393"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,9,9]]},"references-count":72,"journal-issue":{"issue":"9","published-online":{"date-parts":[[2021,9]]}},"alternative-id":["genes12091393"],"URL":"https:\/\/doi.org\/10.3390\/genes12091393","relation":{},"ISSN":["2073-4425"],"issn-type":[{"value":"2073-4425","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,9,9]]}}}