{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,16]],"date-time":"2026-07-16T02:53:42Z","timestamp":1784170422057,"version":"3.55.0"},"reference-count":74,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2021,10,29]],"date-time":"2021-10-29T00:00:00Z","timestamp":1635465600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003627","name":"Rural Development Administration","doi-asserted-by":"publisher","award":["PJ01423702"],"award-info":[{"award-number":["PJ01423702"]}],"id":[{"id":"10.13039\/501100003627","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Genes"],"abstract":"<jats:p>Organophosphate pesticides (OPPs) are one of the most widely used insecticides. OPPs exert their neurotoxic effects by inhibiting acetylcholine esterase (AChE). Most of the gross developmental abnormalities observed in OPP-treated fish, on the other hand, may not be explained solely by AChE inhibition. To understand the overall molecular mechanisms involved in OPP toxicity, we used the zebrafish (ZF) model. We exposed ZF embryos to an OPP, phosmet, for 96 h, and then analyzed developmental abnormalities and performed whole transcriptome analysis. Phenotypic abnormalities, such as bradycardia, spine curvature, and growth retardation, were observed in phosmet-treated ZF (PTZF). Whole transcriptome analysis revealed 2190 differentially expressed genes (DEGs), with 822 and 1368 significantly up-and downregulated genes, respectively. System process and sensory and visual perception were among the top biological pathways affected by phosmet toxicity. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis revealed significant enrichment of metabolic pathways, calcium signaling pathway, regulation of actin cytoskeleton, cardiac muscle contraction, drug metabolism\u2013other enzymes, and phototransduction. Quantitative real-time PCR results of six DEGs agreed with the sequencing data expression profile trend. Our findings provide insights into the consequences of phosmet exposure in ZF, as well as an estimate of the potential risk of OPPs to off-target species.<\/jats:p>","DOI":"10.3390\/genes12111738","type":"journal-article","created":{"date-parts":[[2021,11,1]],"date-time":"2021-11-01T22:21:08Z","timestamp":1635805268000},"page":"1738","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":31,"title":["Transcriptome-Based Identification of Genes Responding to the Organophosphate Pesticide Phosmet in Danio rerio"],"prefix":"10.3390","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5529-418X","authenticated-orcid":false,"given":"Bala Murali Krishna","family":"Vasamsetti","sequence":"first","affiliation":[{"name":"Toxicity and Risk Assessment Division, Department of Agro-Food Safety and Crop Protection, National Institute of Agricultural Sciences, Rural Development Administration, Wanju-gun 55365, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2143-2614","authenticated-orcid":false,"given":"Kyongmi","family":"Chon","sequence":"additional","affiliation":[{"name":"Toxicity and Risk Assessment Division, Department of Agro-Food Safety and Crop Protection, National Institute of Agricultural Sciences, Rural Development Administration, Wanju-gun 55365, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juyeong","family":"Kim","sequence":"additional","affiliation":[{"name":"Toxicity and Risk Assessment Division, Department of Agro-Food Safety and Crop Protection, National Institute of Agricultural Sciences, Rural Development Administration, Wanju-gun 55365, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jin-A","family":"Oh","sequence":"additional","affiliation":[{"name":"Toxicity and Risk Assessment Division, Department of Agro-Food Safety and Crop Protection, National Institute of Agricultural Sciences, Rural Development Administration, Wanju-gun 55365, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chang-Young","family":"Yoon","sequence":"additional","affiliation":[{"name":"Toxicity and Risk Assessment Division, Department of Agro-Food Safety and Crop Protection, National Institute of Agricultural Sciences, Rural Development Administration, Wanju-gun 55365, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hong-Hyun","family":"Park","sequence":"additional","affiliation":[{"name":"Toxicity and Risk Assessment Division, Department of Agro-Food Safety and Crop Protection, National Institute of Agricultural Sciences, Rural Development Administration, Wanju-gun 55365, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2021,10,29]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"268","DOI":"10.3389\/fpubh.2018.00268","article-title":"Toxicity of pesticides on health and environment","volume":"6","author":"Mesnage","year":"2018","journal-title":"Front. Public Health"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"407","DOI":"10.1590\/S0103-50532009000300003","article-title":"Organophosphorus compounds as chemical warfare agents: A review","volume":"20","author":"Delfino","year":"2009","journal-title":"J. Braz. Chem. Soc."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"1119","DOI":"10.1016\/j.chemosphere.2012.03.067","article-title":"Phosphorus flame retardants: Properties, production, environmental occurrence, toxicity and analysis","volume":"88","year":"2012","journal-title":"Chemosphere"},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Ghimire, R., Utyasheva, L., Pokhrel, M., Rai, N., Chaudhary, B., Prasad, P.N., Bajracharya, S.R., Basnet, B., Das, K.D., and Pathak, N.K. (2021). Intentional pesticide poisoning and pesticide suicides in Nepal. Clin. Toxicol., 1\u20137.","DOI":"10.1080\/15563650.2021.1935993"},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Karunarathne, A., Bhalla, A., Sethi, A., Perera, U., and Eddleston, M. (2021). Importance of pesticides for lethal poisoning in India during 1999 to 2018: A systematic review. BMC Public Health, 21.","DOI":"10.1186\/s12889-021-11156-2"},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Hertz-Picciotto, I., Sass, J.B., Engel, S., Bennett, D.H., Bradman, A., Eskenazi, B., Lanphear, B., and Whyatt, R. (2018). Organophosphate exposures during pregnancy and child neurodevelopment: Recommendations for essential policy reforms. PLoS Med., 15.","DOI":"10.1371\/journal.pmed.1002671"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"158","DOI":"10.1016\/j.neuro.2013.09.003","article-title":"Neurodevelopmental effects in children associated with exposure to organophosphate pesticides: A systematic review","volume":"39","author":"Lucero","year":"2013","journal-title":"Neurotoxicology"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1080\/03601234.2019.1660562","article-title":"Teratological effects of pesticides in vertebrates: A review","volume":"55","author":"Pires","year":"2020","journal-title":"J. Environ. Sci. Health Part B"},{"key":"ref_9","unstructured":"Directorate of Plant Protection, Quarantine and Storage, India (2021, October 19). Pesticide Wise Consumption of Indigenous Pesticides. 2021, Available online: http:\/\/ppqs.gov.in\/sites\/default\/files\/3.pesticidewise_consumption_of_indiginous_pesticides.xlsx."},{"key":"ref_10","unstructured":"(2021, June 21). US EPA. 2006. Reregistration Eligibility Decision, Phosmet, Available online: https:\/\/www3.epa.gov\/pesticides\/chem_search\/reg_actions\/reregistration\/red_PC-059201_31-Jul-06.pdf."},{"key":"ref_11","unstructured":"(2021, October 21). Pesticide Info Data Base. California Pesticide Use, 2000\u20132018. Available online: https:\/\/www.pesticideinfo.org\/california-search?title=Phosmet,All,All&chk=335&sk=all&cok=all&minyr=2000&maxyr=2018."},{"key":"ref_12","first-page":"791","article-title":"Embryotoxicity and teratogenicity of phosmet in mice","volume":"34","author":"Bleyl","year":"1980","journal-title":"Arch. Fur Exp. Vet."},{"key":"ref_13","unstructured":"(2021, October 21). US EPA, 2010. Risks of Phosmet Use to the Federally Threatened and Endangered California Tiger Salamander (Ambystoma californiense), Available online: https:\/\/www3.epa.gov\/pesticides\/endanger\/litstatus\/effects\/redleg-frog\/2010\/phosmet\/assessment.pdf."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"196","DOI":"10.1016\/j.cbpc.2008.11.006","article-title":"Is the fish embryo toxicity test (FET) with the zebrafish (Danio rerio) a potential alternative for the fish acute toxicity test?","volume":"149","author":"Lammer","year":"2009","journal-title":"Comp. Biochem. Physiol. Part C Toxicol. Pharmacol."},{"key":"ref_15","first-page":"38","article-title":"DarT: The embryo test with the Zebrafish Danio rerio\u2014A general model in ecotoxicology and toxicology","volume":"19","author":"Nagel","year":"2002","journal-title":"Altex"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"498","DOI":"10.1038\/nature12111","article-title":"The zebrafish reference genome sequence and its relationship to the human genome","volume":"496","author":"Howe","year":"2013","journal-title":"Nature"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"136","DOI":"10.1897\/04-195R.1","article-title":"Comparative thresholds for acetylcholinesterase inhibition and behavioral impairment in coho salmon exposed to chlorpyrifos","volume":"24","author":"Sandahl","year":"2005","journal-title":"Environ. Toxicol. Chem. Int. J."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"4996","DOI":"10.1021\/es800240u","article-title":"Salmon olfaction is impaired by an environmentally realistic pesticide mixture","volume":"42","author":"Tierney","year":"2008","journal-title":"Environ. Sci. Technol."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"343","DOI":"10.7585\/kjps.2020.24.4.343","article-title":"Developmental Toxic Effects of Phosmet on Zebrafish (Danio rerio) Embryos","volume":"24","author":"Vasamsetti","year":"2020","journal-title":"Korean J. Pestic. Sci."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"317","DOI":"10.1016\/j.toxrep.2020.02.004","article-title":"Toxicity of the organophosphate insecticide sumithion to embryo and larvae of zebrafish","volume":"7","author":"Rahman","year":"2020","journal-title":"Toxicol. Rep."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"567","DOI":"10.1177\/0748233710373089","article-title":"Dichlorvos-induced developmental toxicity in zebrafish","volume":"26","year":"2010","journal-title":"Toxicol. Ind. Health"},{"key":"ref_22","first-page":"1","article-title":"Biochemical toxicity of organophosphate compounds in fishes","volume":"16","author":"Qayoom","year":"2014","journal-title":"SKUAST J. Res."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"105424","DOI":"10.1016\/j.aquatox.2020.105424","article-title":"Sublethal neurotoxicity of organophosphate insecticides to juvenile coho salmon","volume":"221","author":"Laetz","year":"2020","journal-title":"Aquat. Toxicol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1016\/j.ntt.2019.04.002","article-title":"Comparative analyses of the neurobehavioral, molecular, and enzymatic effects of organophosphates on embryo-larval zebrafish (Danio rerio)","volume":"73","author":"Schmitt","year":"2019","journal-title":"Neurotoxicol. Teratol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"15591","DOI":"10.1038\/srep15591","article-title":"Zebrafish models for human acute organophosphorus poisoning","volume":"5","author":"Faria","year":"2015","journal-title":"Sci. Rep."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"1231","DOI":"10.1016\/j.envpol.2016.11.010","article-title":"Assessment of chlorpyrifos toxic effects in zebrafish (Danio rerio) metabolism","volume":"220","author":"Prats","year":"2017","journal-title":"Environ. Pollut."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"137062","DOI":"10.1016\/j.scitotenv.2020.137062","article-title":"Single and joint toxic effects of four antibiotics on some metabolic pathways of zebrafish (Danio rerio) larvae","volume":"716","author":"Qiu","year":"2020","journal-title":"Sci. Total. Environ."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"126148","DOI":"10.1016\/j.chemosphere.2020.126148","article-title":"Phenotypic and transcriptomic changes in zebrafish (Danio rerio) embryos\/larvae following cypermethrin exposure","volume":"249","author":"Ranjani","year":"2020","journal-title":"Chemosphere"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"8083","DOI":"10.1038\/s41598-018-26499-x","article-title":"Toxicity and transcriptome sequencing (RNA-seq) analyses of adult zebrafish in response to exposure carboxymethyl cellulose stabilized iron sulfide nanoparticles","volume":"8","author":"Zheng","year":"2018","journal-title":"Sci. Rep."},{"key":"ref_30","first-page":"1101","article-title":"Pesticide toxicity: A mechanistic approach","volume":"17","author":"Lushchak","year":"2018","journal-title":"EXCLI J."},{"key":"ref_31","doi-asserted-by":"crossref","unstructured":"Park, M.Y., Krishna Vasamsetti, B.M., Kim, W.S., Kang, H.J., Kim, D.-Y., Lim, B., Cho, K., Kim, J.S., Chee, H.K., and Park, J.H. (2021). Comprehensive Analysis of Cardiac Xeno-Graft Unveils Rejection Mechanisms. Int. J. Mol. Sci., 22.","DOI":"10.3390\/ijms22020751"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"47","DOI":"10.1186\/s41936-020-00184-w","article-title":"Toxic effects of an organophosphate pesticide, envoy 50 SC on the histopathological, hematological, and brain acetylcholinesterase activities in stinging catfish (Heteropneustes fossilis)","volume":"81","author":"Akter","year":"2020","journal-title":"J. Basic Appl. Zool."},{"key":"ref_33","first-page":"19","article-title":"Impacts of oxidative stress on acetylcholinesterase transcription, and activity in embryos of zebrafish (Danio rerio) following Chlorpyrifos exposure","volume":"172","author":"Schlenk","year":"2015","journal-title":"Comp. Biochem. Physiol. Part C Toxicol. Pharmacol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"50","DOI":"10.1016\/j.neuro.2015.05.002","article-title":"Chlorpyrifos and malathion have opposite effects on behaviors and brain size that are not correlated to changes in AChE activity","volume":"49","author":"Richendrfer","year":"2015","journal-title":"Neurotoxicology"},{"key":"ref_35","doi-asserted-by":"crossref","unstructured":"Bui-Nguyen, T.M., Baer, C.E., Lewis, J.A., Yang, D., Lein, P.J., and Jackson, D.A. (2015). Dichlorvos exposure results in large scale disruption of energy metabolism in the liver of the zebrafish, Danio rerio. BMC Genom., 16.","DOI":"10.1186\/s12864-015-1941-2"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"377","DOI":"10.1093\/icb\/21.2.377","article-title":"Energy metabolism in fish development","volume":"21","author":"Boulekbache","year":"1981","journal-title":"Am. Zool."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1016\/j.taap.2013.04.001","article-title":"Mitochondrial dysfunction and organophosphorus compounds","volume":"270","author":"Abdollahi","year":"2013","journal-title":"Toxicol. Appl. Pharmacol."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1111\/nyas.13115","article-title":"The role of oxidative stress in organophosphate and nerve agent toxicity","volume":"1378","author":"Pearson","year":"2016","journal-title":"Ann. N. Y. Acad. Sci."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"1252","DOI":"10.1016\/j.freeradbiomed.2012.07.021","article-title":"Cytochrome c oxidase dysfunction in oxidative stress","volume":"53","author":"Srinivasan","year":"2012","journal-title":"Free. Radic. Biol. Med."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"34839","DOI":"10.1074\/jbc.M703528200","article-title":"Early developmental pathology due to cytochrome c oxidase deficiency is revealed by a new zebrafish model","volume":"282","author":"Baden","year":"2007","journal-title":"J. Biol. Chem."},{"key":"ref_41","doi-asserted-by":"crossref","unstructured":"Di Giulio, R.T., and Hinton, D.E. (2008). The Toxicology of Fishes, CRC Press.","DOI":"10.1201\/9780203647295"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"113","DOI":"10.1002\/(SICI)1521-1878(200002)22:2<113::AID-BIES3>3.0.CO;2-L","article-title":"Calcium signalling during zebrafish embryonic development","volume":"22","author":"Webb","year":"2000","journal-title":"Bioessays"},{"key":"ref_43","doi-asserted-by":"crossref","unstructured":"Paudel, S., Sindelar, R., and Saha, M. (2018). Calcium signaling in vertebrate development and its role in disease. Int. J. Mol. Sci., 19.","DOI":"10.3390\/ijms19113390"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1016\/j.devbrainres.2004.03.016","article-title":"The calcium pump of the endoplasmic reticulum plays a role in midline signaling during early zebrafish development","volume":"151","author":"Creton","year":"2004","journal-title":"Dev. Brain Res."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"18","DOI":"10.1016\/j.ntt.2018.10.001","article-title":"Biological impacts of organophosphates chlorpyrifos and diazinon on development, mitochondrial bioenergetics, and locomotor activity in zebrafish (Danio rerio)","volume":"70","author":"Cao","year":"2018","journal-title":"Neurotoxicology Teratol."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"161","DOI":"10.1016\/B978-0-12-387036-0.00007-4","article-title":"A guide to analysis of cardiac phenotypes in the zebrafish embryo","volume":"101","author":"Miura","year":"2011","journal-title":"Methods Cell Biol."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"889","DOI":"10.1152\/physrev.00038.2016","article-title":"Little fish, big data: Zebrafish as a model for cardiovascular and metabolic disease","volume":"97","author":"Gut","year":"2017","journal-title":"Physiol. Rev."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"2829","DOI":"10.1038\/ncomms3829","article-title":"MiR-1 and miR-206 target different genes to have opposing roles during angiogenesis in zebrafish embryos","volume":"4","author":"Lin","year":"2013","journal-title":"Nat. Commun."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"106","DOI":"10.1038\/ng875","article-title":"Cardiac troponin T is essential in sarcomere assembly and cardiac contractility","volume":"31","author":"Sehnert","year":"2002","journal-title":"Nat. Genet."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"205","DOI":"10.1038\/ng816","article-title":"Cardiomyopathy in zebrafish due to mutation in an alternatively spliced exon of titin","volume":"30","author":"Xu","year":"2002","journal-title":"Nat. Genet."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"2138","DOI":"10.1016\/j.cub.2003.11.055","article-title":"Heart of glass regulates the concentric growth of the heart in zebrafish","volume":"13","author":"Mably","year":"2003","journal-title":"Curr. Biol."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"e000231","DOI":"10.1161\/JAHA.113.000231","article-title":"Inactivation of Myosin binding protein C homolog in zebrafish as a model for human cardiac hypertrophy and diastolic dysfunction","volume":"2","author":"Chen","year":"2013","journal-title":"J. Am. Heart Assoc."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"7636","DOI":"10.1073\/pnas.0902919106","article-title":"Ablation of triadin causes loss of cardiac Ca2+ release units, impaired excitation\u2013contraction coupling, and cardiac arrhythmias","volume":"106","author":"Chopra","year":"2009","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"572","DOI":"10.1161\/CIRCRESAHA.114.301275","article-title":"Integrins and integrin-associated proteins in the cardiac myocyte","volume":"114","author":"Manso","year":"2014","journal-title":"Circ. Res."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"1905","DOI":"10.1002\/cbic.201900741","article-title":"Cyp1 inhibition prevents doxorubicin-induced cardiomyopathy in a zebrafish heart failure model","volume":"21","author":"Lam","year":"2020","journal-title":"Chembiochem A Eur. J. Chem. Biol."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"1613","DOI":"10.1242\/jcs.111.12.1613","article-title":"Patterns of free calcium in zebrafish embryos","volume":"111","author":"Speksnijder","year":"1998","journal-title":"J. Cell Sci."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"199","DOI":"10.1016\/j.yjmcc.2013.01.015","article-title":"Polycystin-2 mutations lead to impaired calcium cycling in the heart and predispose to dilated cardiomyopathy","volume":"58","author":"Paavola","year":"2013","journal-title":"J. Mol. Cell. Cardiol."},{"key":"ref_58","first-page":"9","article-title":"Cardiological manifestations of mitochondrial respiratory chain disorders","volume":"30","author":"Berardo","year":"2011","journal-title":"Acta Myol."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"1072","DOI":"10.1111\/bph.12186","article-title":"How mitochondrial dysfunction affects zebrafish development and cardiovascular function: An in vivo model for testing mitochondria-targeted drugs","volume":"169","author":"Pinho","year":"2013","journal-title":"Br. J. Pharmacol."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"102","DOI":"10.1265\/ehpm.11.102","article-title":"Ocular toxicity from pesticide exposure: A recent review","volume":"11","author":"Jaga","year":"2006","journal-title":"Environ. Health Prev. Med."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"3031","DOI":"10.1111\/j.1742-4658.2008.06455.x","article-title":"The evolutionary relationship between the duplicated copies of the zebrafish fabp11 gene and the tetrapod FABP4, FABP5, FABP8 and FABP9 genes","volume":"275","author":"Karanth","year":"2008","journal-title":"FEBS J."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"150086","DOI":"10.1098\/rsob.150086","article-title":"Recoverin depletion accelerates cone photoresponse recovery","volume":"5","author":"Zang","year":"2015","journal-title":"Open Biol."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"104","DOI":"10.1016\/j.exer.2015.07.001","article-title":"A conserved role of \u03b1A-crystallin in the development of the zebrafish embryonic lens","volume":"138","author":"Zou","year":"2015","journal-title":"Exp. Eye Res."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"108535","DOI":"10.1016\/j.exer.2021.108535","article-title":"Single cell transcriptomics of the developing zebrafish lens and identification of putative controllers of lens development","volume":"206","author":"Farnsworth","year":"2021","journal-title":"Exp. Eye Res."},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"8392","DOI":"10.1038\/s41598-021-87879-4","article-title":"Leucyl-tRNA synthetase deficiency systemically induces excessive autophagy in zebrafish","volume":"11","author":"Inoue","year":"2021","journal-title":"Sci. Rep."},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"378","DOI":"10.1016\/j.bbrc.2018.09.133","article-title":"Loss of Leucyl-tRNA synthetase b leads to ILFS1-like symptoms in zebrafish","volume":"505","author":"Wang","year":"2018","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"67","DOI":"10.3389\/fcell.2019.00067","article-title":"Knock-down of histidyl-tRNA synthetase causes cell cycle arrest and apoptosis of neuronal progenitor cells in vivo","volume":"7","author":"Waldron","year":"2019","journal-title":"Front. Cell Dev. Biol."},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"93","DOI":"10.1016\/j.mrgentox.2008.09.009","article-title":"Polymorphisms in the NQO1, GSTT and GSTM genes are associated with coronary heart disease and biomarkers of oxidative stress","volume":"674","author":"Martin","year":"2009","journal-title":"Mutat. Res.\/Genet. Toxicol. Environ. Mutagenes."},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"40","DOI":"10.1016\/j.pediatrneurol.2019.02.008","article-title":"6-Pyruvoyltetrahydropterin synthase deficiency: Review and report of 28 Arab subjects","volume":"96","author":"Almannai","year":"2019","journal-title":"Pediatric Neurol."},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"724","DOI":"10.1111\/pcmr.12783","article-title":"Larval but not adult xanthophore pigmentation in zebrafish requires GTP cyclohydrolase 2 (gch2) function","volume":"32","author":"Lister","year":"2019","journal-title":"Pigment. Cell Melanoma Res."},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"439","DOI":"10.1007\/s00210-011-0619-9","article-title":"The nme gene family in zebrafish oogenesis and early development","volume":"384","author":"Desvignes","year":"2011","journal-title":"Naunyn-Schmiedeberg\u2019s Arch. Pharacol."},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"62","DOI":"10.1124\/mol.113.091462","article-title":"Characterization of the zebrafish Ugt repertoire reveals a new class of drug-metabolizing UDP glucuronosyltransferases","volume":"86","author":"Wang","year":"2014","journal-title":"Mol. Pharmacol."},{"key":"ref_73","doi-asserted-by":"crossref","first-page":"128","DOI":"10.1016\/j.reprotox.2011.06.121","article-title":"Zebrafish embryos as an alternative to animal experiments\u2014A commentary on the definition of the onset of protected life stages in animal welfare regulations","volume":"33","author":"Scholz","year":"2012","journal-title":"Reprod. Toxicol."},{"key":"ref_74","doi-asserted-by":"crossref","first-page":"80","DOI":"10.1186\/s13765-020-00566-2","article-title":"Teratogenic and developmental toxic effects of etridiazole on zebrafish (Danio rerio) embryos","volume":"63","author":"Vsamsetti","year":"2020","journal-title":"Appl. Biol. 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