{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T13:29:52Z","timestamp":1791811792026,"version":"4.3.4"},"reference-count":38,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2021,10,30]],"date-time":"2021-10-30T00:00:00Z","timestamp":1635552000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81930066, 81871727, 81572330, and 81772941"],"award-info":[{"award-number":["81930066, 81871727, 81572330, and 81772941"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Shanghai Tenth People's Hospital Climbing Talents Program","award":["2021SYPDRC023"],"award-info":[{"award-number":["2021SYPDRC023"]}]}],"content-domain":{"domain":["www.mdpi.com"],"crossmark-restriction":true},"short-container-title":["Genes"],"abstract":"<jats:p>The N6-methyladenosine (m6A) RNA modification can regulate autophagy to modulate the growth and development of tumors, but the mechanism of m6A modification for the regulation of autophagy in hepatocellular carcinoma cells (HCC) remains unclear. In the study, the knockdown of the Wilms\u2019 tumor 1-associating protein (WTAP) was made in HCC to study the correlation between m6A modification and autophagy. A fluorescent confocal microscopy analysis showed that the knockdown of WTAP could facilitate the autophagy of HCC. A Western blot analysis showed that the level of p-AMPK was decreased in WTAP-knockdown HCC cells. Additionally, LKB1, the upstream kinase of AMPK, was regulated by WTAP and it could mediate the phosphorylation of AMPK in an m6A-dependent manner. Further studies revealed that the knockdown of WTAP could reduce the level of LKB1 mRNA with m6A. This could result in the increased stability of LKB1 mRNA to promote its expression. The knockdown of WTAP could upregulate the level of autophagy and inhibit HCC proliferation. However, the overexpression of WTAP could resist autophagic cell death.<\/jats:p>","DOI":"10.3390\/genes12111747","type":"journal-article","created":{"date-parts":[[2021,11,1]],"date-time":"2021-11-01T22:21:08Z","timestamp":1635805268000},"page":"1747","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":36,"title":["m6A mRNA Methylation Regulates LKB1 to Promote Autophagy of Hepatoblastoma Cells through Upregulated Phosphorylation of AMPK"],"prefix":"10.3390","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4214-0533","authenticated-orcid":false,"given":"Guohui","family":"Li","sequence":"first","affiliation":[{"name":"School of Life Sciences, Jiangsu University, Zhenjiang 212013, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liang","family":"Deng","sequence":"additional","affiliation":[{"name":"School of Life Sciences, Jiangsu University, Zhenjiang 212013, China"},{"name":"Department of Clinical Laboratory Medicine, Shanghai Tenth People\u2019s Hospital of Tongji University, Shanghai 200072, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nan","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory Medicine, Shanghai Tenth People\u2019s Hospital of Tongji University, Shanghai 200072, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhongqi","family":"Cui","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory Medicine, Shanghai Tenth People\u2019s Hospital of Tongji University, Shanghai 200072, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qi","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory Medicine, Shanghai Tenth People\u2019s Hospital of Tongji University, Shanghai 200072, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6904-5360","authenticated-orcid":false,"given":"Ji","family":"Ma","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, Shanghai Children\u2019s Medical Center, Shanghai Jiao Tong University School of Medicine, Shanghai 200072, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiuhui","family":"Pan","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, Shanghai Children\u2019s Medical Center, Shanghai Jiao Tong University School of Medicine, Shanghai 200072, China"},{"name":"Shanghai Key Laboratory of Clinical Molecular Diagnostics for Pediatrics, Shanghai 200072, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fenyong","family":"Sun","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory Medicine, Shanghai Tenth People\u2019s Hospital of Tongji University, Shanghai 200072, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2021,10,30]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1007\/s15010-019-01345-y","article-title":"Epidemiological and etiological variations in hepatocellular carcinoma","volume":"48","author":"Sagnelli","year":"2020","journal-title":"Infection"},{"key":"ref_2","first-page":"209","article-title":"Global Cancer Statistics 2020: GLOBOCAN Estimates of Incidence and Mortality Worldwide for 36 Cancers in 185 Countries","volume":"71","author":"Sung","year":"2021","journal-title":"CA Cancer J. 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