{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,18]],"date-time":"2026-07-18T00:57:18Z","timestamp":1784336238055,"version":"3.55.0"},"reference-count":29,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2021,12,2]],"date-time":"2021-12-02T00:00:00Z","timestamp":1638403200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003407","name":"Ministry of Education, Universities and Research","doi-asserted-by":"publisher","award":["PRIN2017F8ZB89"],"award-info":[{"award-number":["PRIN2017F8ZB89"]}],"id":[{"id":"10.13039\/501100003407","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003407","name":"Ministry of Education, Universities and Research","doi-asserted-by":"publisher","award":["VALERE: Vanvitelli Project 2020"],"award-info":[{"award-number":["VALERE: Vanvitelli Project 2020"]}],"id":[{"id":"10.13039\/501100003407","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Italian Ministry of Health","award":["GR-2016-02364785"],"award-info":[{"award-number":["GR-2016-02364785"]}]},{"name":"Italian Ministry of Health","award":["Ricerca Corrente (RC)"],"award-info":[{"award-number":["Ricerca Corrente (RC)"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Genes"],"abstract":"<jats:p>Objectives: Dilated cardiomyopathy (DCM) is characterized by a specific transcriptome. Since the DCM molecular network is largely unknown, the aim was to identify specific disease-related molecular targets combining an original machine learning (ML) approach with protein-protein interaction network. Methods: The transcriptomic profiles of human myocardial tissues were investigated integrating an original computational approach, based on the Custom Decision Tree algorithm, in a differential expression bioinformatic framework. Validation was performed by quantitative real-time PCR. Results: Our preliminary study, using samples from transplanted tissues, allowed the discovery of specific DCM-related genes, including MYH6, NPPA, MT-RNR1 and NEAT1, already known to be involved in cardiomyopathies Interestingly, a combination of these expression profiles with clinical characteristics showed a significant association between NEAT1 and left ventricular end-diastolic diameter (LVEDD) (Rho = 0.73, p = 0.05), according to severity classification (NYHA-class III). Conclusions: The use of the ML approach was useful to discover preliminary specific genes that could lead to a rapid selection of molecular targets correlated with DCM clinical parameters. For the first time, NEAT1 under-expression was significantly associated with LVEDD in the human heart.<\/jats:p>","DOI":"10.3390\/genes12121946","type":"journal-article","created":{"date-parts":[[2021,12,2]],"date-time":"2021-12-02T21:19:08Z","timestamp":1638479948000},"page":"1946","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Machine Learning and Bioinformatics Framework Integration to Potential Familial DCM-Related Markers Discovery"],"prefix":"10.3390","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2570-6910","authenticated-orcid":false,"given":"Concetta","family":"Schiano","sequence":"first","affiliation":[{"name":"Department of Advanced Medical and Surgical Sciences (DAMSS), University of Campania \u201cLuigi Vanvitelli\u201d, 80138 Naples, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6490-7694","authenticated-orcid":false,"given":"Monica","family":"Franzese","sequence":"additional","affiliation":[{"name":"IRCCS SDN, 80121 Naples, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6993-6761","authenticated-orcid":false,"given":"Filippo","family":"Geraci","sequence":"additional","affiliation":[{"name":"Institute for Informatics and Telematics, CNR, 56124 Pisa, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mario","family":"Zanfardino","sequence":"additional","affiliation":[{"name":"IRCCS SDN, 80121 Naples, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ciro","family":"Maiello","sequence":"additional","affiliation":[{"name":"Department of Cardiovascular Surgery and Transplant, Azienda dei Colli, Monaldi Hospital, 80131 Naples, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vittorio","family":"Palmieri","sequence":"additional","affiliation":[{"name":"Department of Cardiovascular Surgery and Transplant, Azienda dei Colli, Monaldi Hospital, 80131 Naples, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrea","family":"Soricelli","sequence":"additional","affiliation":[{"name":"IRCCS SDN, 80121 Naples, Italy"},{"name":"Department of Exercise and Wellness Sciences, University of Naples Parthenope, 80133 Naples, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vincenzo","family":"Grimaldi","sequence":"additional","affiliation":[{"name":"IRCCS SDN, 80121 Naples, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Enrico","family":"Coscioni","sequence":"additional","affiliation":[{"name":"Division of Cardiac Surgery, AOU San Giovanni di Dio e Ruggid\u2019Aragona, 84131 Salerno, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marco","family":"Salvatore","sequence":"additional","affiliation":[{"name":"IRCCS SDN, 80121 Naples, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5455-555X","authenticated-orcid":false,"given":"Claudio","family":"Napoli","sequence":"additional","affiliation":[{"name":"Department of Advanced Medical and Surgical Sciences (DAMSS), University of Campania \u201cLuigi Vanvitelli\u201d, 80138 Naples, Italy"},{"name":"IRCCS SDN, 80121 Naples, Italy"},{"name":"Clinical Department of Internal Medicine and Specialistic Units, Division of Clinical Immunology, Immunohematology, Transfusion Medicine and Transplant Immunology (SIMT), Azienda Universitaria Policlinico (AOU), Regional Reference Laboratory of Transplant Immunology (LIT), 80131 Naples, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2021,12,2]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"640","DOI":"10.2459\/JCM.0000000000000834","article-title":"Unresolved issues in left ventricular postischemic remodeling and progression to heart failure","volume":"20","author":"Gronda","year":"2019","journal-title":"J. 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