{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,14]],"date-time":"2026-01-14T16:35:55Z","timestamp":1768408555770,"version":"3.49.0"},"reference-count":66,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2022,1,15]],"date-time":"2022-01-15T00:00:00Z","timestamp":1642204800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000015","name":"United States Department of Energy","doi-asserted-by":"publisher","award":["DE-AC02-05CH11231"],"award-info":[{"award-number":["DE-AC02-05CH11231"]}],"id":[{"id":"10.13039\/100000015","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000015","name":"United States Department of Energy","doi-asserted-by":"publisher","award":["KP\/CH57\/1"],"award-info":[{"award-number":["KP\/CH57\/1"]}],"id":[{"id":"10.13039\/100000015","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.mdpi.com"],"crossmark-restriction":true},"short-container-title":["Genes"],"abstract":"<jats:p>Aerobic bacteria that degrade methylphosphonates and produce methane as a byproduct have emerged as key players in marine carbon and phosphorus cycles. Here, we present two new draft genome sequences of the genus Marivita that were assembled from metagenomes from hypersaline former industrial salterns and compare them to five other Marivita reference genomes. Phylogenetic analyses suggest that both of these metagenome-assembled genomes (MAGs) represent new species in the genus. Average nucleotide identities to the closest taxon were &lt;85%. The MAGs were assembled with SPAdes, binned with MetaBAT, and curated with scaffold extension and reassembly. Both genomes contained the phnCDEGHIJLMP suite of genes encoding the full C-P lyase pathway of methylphosphonate degradation and were significantly more abundant in two former industrial salterns than in nearby reference and restored wetlands, which have lower salinity levels and lower methane emissions than the salterns. These organisms contain a variety of compatible solute biosynthesis and transporter genes to cope with high salinity levels but harbor only slightly acidic proteomes (mean isoelectric point of 6.48).<\/jats:p>","DOI":"10.3390\/genes13010148","type":"journal-article","created":{"date-parts":[[2022,1,16]],"date-time":"2022-01-16T20:44:00Z","timestamp":1642365840000},"page":"148","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Methylphosphonate Degradation and Salt-Tolerance Genes of Two Novel Halophilic Marivita Metagenome-Assembled Genomes from Unrestored Solar Salterns"],"prefix":"10.3390","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2565-7100","authenticated-orcid":false,"given":"Clifton","family":"Bueno de Mesquita","sequence":"first","affiliation":[{"name":"Department of Energy Joint Genome Institute, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jinglie","family":"Zhou","sequence":"additional","affiliation":[{"name":"Department of Energy Joint Genome Institute, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9812-7856","authenticated-orcid":false,"given":"Susanna","family":"Theroux","sequence":"additional","affiliation":[{"name":"Southern California Coastal Water Research Project, Costa Mesa, CA 92626, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6479-8427","authenticated-orcid":false,"given":"Susannah","family":"Tringe","sequence":"additional","affiliation":[{"name":"Department of Energy Joint Genome Institute, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA"},{"name":"Environmental Genomics and Systems Biology Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2022,1,15]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"473","DOI":"10.1038\/ngeo234","article-title":"Aerobic Production of Methane in the Sea","volume":"1","author":"Karl","year":"2008","journal-title":"Nat. Geosci."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"93","DOI":"10.3354\/ame01714","article-title":"Aerobic Production of Methane from Dissolved Water-Column Methylphosphonate and Sinking Particles in the North Pacific Subtropical Gyre","volume":"73","author":"Karl","year":"2014","journal-title":"Aquat. Microb. Ecol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"884","DOI":"10.1038\/ngeo2837","article-title":"Marine Methane Paradox Explained by Bacterial Degradation of Dissolved Organic Matter","volume":"9","author":"Repeta","year":"2016","journal-title":"Nat. Geosci."},{"key":"ref_4","first-page":"340","article-title":"Metatranscriptomic and Functional Metagenomic Analysis of Methylphosphonate Utilization by Marine Bacteria","volume":"4","author":"Ventouras","year":"2013","journal-title":"Front. Microbiol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"6994","DOI":"10.1128\/AEM.02399-16","article-title":"Freshwater Bacteria Release Methane as a By-Product of Phosphorus Acquisition","volume":"82","author":"Yao","year":"2016","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"e2019229118","DOI":"10.1073\/pnas.2019229118","article-title":"Aerobic Bacterial Methane Synthesis","volume":"118","author":"Wang","year":"2021","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"570","DOI":"10.1038\/nature10622","article-title":"Intermediates in the Transformation of Phosphonates to Phosphate by Bacteria","volume":"480","author":"Kamat","year":"2011","journal-title":"Nature"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"132","DOI":"10.1038\/nature12061","article-title":"The Catalytic Mechanism for Aerobic Formation of Methane by Bacteria","volume":"497","author":"Kamat","year":"2013","journal-title":"Nature"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"1104","DOI":"10.1126\/science.1219875","article-title":"Synthesis of Methylphosphonic Acid by Marine Microbes: A Source for Methane in the Aerobic Ocean","volume":"337","author":"Metcalf","year":"2012","journal-title":"Science"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"1336","DOI":"10.1126\/science.aao3435","article-title":"Structural Basis for Methylphosphonate Biosynthesis","volume":"358","author":"Born","year":"2017","journal-title":"Science"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"2402","DOI":"10.1111\/1462-2920.14628","article-title":"Phosphate-Limited Ocean Regions Select for Bacterial Populations Enriched in the Carbon-Phosphorus Lyase Pathway for Phosphonate Degradation","volume":"21","author":"Sosa","year":"2019","journal-title":"Environ. Microbiol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"e01718-20","DOI":"10.1128\/mBio.01718-20","article-title":"The Role of Phosphorus Limitation in Shaping Soil Bacterial Communities and Their Metabolic Capabilities","volume":"11","author":"Oliverio","year":"2020","journal-title":"mBio"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"4346","DOI":"10.1038\/ncomms5346","article-title":"Methane Production by Phosphate-Starved SAR11 Chemoheterotrophic Marine Bacteria","volume":"5","author":"Carini","year":"2014","journal-title":"Nat. Commun."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"1619","DOI":"10.1038\/s41396-018-0056-6","article-title":"Strains of the Toxic and Bloom-Forming Nodularia Spumigena (Cyanobacteria) Can Degrade Methylphosphonate and Release Methane","volume":"12","author":"Teikari","year":"2018","journal-title":"ISME J."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"284","DOI":"10.1038\/s41396-021-01067-w","article-title":"Microbial Drivers of Methane Emissions from Unrestored Industrial Salt Ponds","volume":"16","author":"Zhou","year":"2021","journal-title":"ISME J."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"D666","DOI":"10.1093\/nar\/gky901","article-title":"IMG\/M v.5.0: An Integrated Data Management and Comparative Analysis System for Microbial Genomes and Microbiomes","volume":"47","author":"Chen","year":"2019","journal-title":"Nucleic Acids Res."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"1568","DOI":"10.1099\/ijs.0.005462-0","article-title":"Marivita Cryptomonadis Gen. Nov., Sp. Nov. and Marivita Litorea Sp. Nov., of the Family Rhodobacteraceae, Isolated from Marine Habitats","volume":"59","author":"Hwang","year":"2009","journal-title":"Int. J. Syst. Evol. Microbiol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"839","DOI":"10.1099\/ijs.0.032086-0","article-title":"Marivita Hallyeonensis Sp. Nov., Isolated from Seawater, Reclassification of Gaetbulicola Byunsanensis as Marivita Byunsanensis Comb. Nov. and Emended Description of the Genus Marivita","volume":"62","author":"Yoon","year":"2012","journal-title":"Int. J. Syst. Evol. Microbiol."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"423","DOI":"10.1099\/ijs.0.039065-0","article-title":"Marivita Geojedonensis Sp. Nov., Isolated from Seawater","volume":"63","author":"Yoon","year":"2013","journal-title":"Int. J. Syst. Evol. Microbiol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"196","DOI":"10.1099\/ijs.0.011015-0","article-title":"Gaetbulicola Byunsanensis Gen. Nov., Sp. Nov., Isolated from Tidal Flat Sediment","volume":"60","author":"Yoon","year":"2010","journal-title":"Int. J. Syst. Evol. Microbiol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"259","DOI":"10.2323\/jgam.57.259","article-title":"Marivita Roseacus Sp. Nov., of the Family Rhodobacteraceae, Isolated from a Temperate Estuary and an Emended Description of the Genus Marivita","volume":"57","author":"Budinoff","year":"2011","journal-title":"J. Gen. Appl. Microbiol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"1889","DOI":"10.1099\/ijs.0.000195","article-title":"Marivita Lacus Sp. Nov., Isolated from a Saline Lake","volume":"65","author":"Zhong","year":"2015","journal-title":"Int. J. Syst. Evol. Microbiol."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"739","DOI":"10.1007\/s42770-021-00463-w","article-title":"Combined Characterization of a New Member of Marivita Cryptomonadis Strain LZ-15-2 Isolated from Cultivable Phycosphere Microbiota of Highly Toxic HAB Dinoflagellate Alexandrium Catenella LZT09","volume":"52","author":"Zhou","year":"2021","journal-title":"Braz. J. Microbiol."},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Bueno de Mesquita, C.P., Zhou, J., Theroux, S.M., and Tringe, S.G. (2021). Methanogenesis and Salt Tolerance Genes of a Novel Halophilic Methanosarcinaceae Metagenome-Assembled Genome from a Former Solar Saltern. Genes, 12.","DOI":"10.3390\/genes12101609"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"e1165","DOI":"10.7717\/peerj.1165","article-title":"MetaBAT, an Efficient Tool for Accurately Reconstructing Single Genomes from Complex Microbial Communities","volume":"3","author":"Kang","year":"2015","journal-title":"PeerJ"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"1043","DOI":"10.1101\/gr.186072.114","article-title":"CheckM: Assessing the Quality of Microbial Genomes Recovered from Isolates, Single Cells, and Metagenomes","volume":"25","author":"Parks","year":"2015","journal-title":"Genome Res."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"725","DOI":"10.1038\/nbt.3893","article-title":"Minimum Information about a Single Amplified Genome (MISAG) and a Metagenome-Assembled Genome (MIMAG) of Bacteria and Archaea","volume":"35","author":"Bowers","year":"2017","journal-title":"Nat. Biotechnol."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"217","DOI":"10.1186\/s13059-019-1817-x","article-title":"Robust Taxonomic Classification of Uncharted Microbial Sequences and Bins with CAT and BAT","volume":"20","author":"Arkhipova","year":"2019","journal-title":"Genome Biol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"1925","DOI":"10.1093\/bioinformatics\/btz848","article-title":"GTDB-Tk: A Toolkit to Classify Genomes with the Genome Taxonomy Database","volume":"36","author":"Chaumeil","year":"2020","journal-title":"Bioinformatics"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"1647","DOI":"10.1093\/bioinformatics\/bts199","article-title":"Geneious Basic: An Integrated and Extendable Desktop Software Platform for the Organization and Analysis of Sequence Data","volume":"28","author":"Kearse","year":"2012","journal-title":"Bioinformatics"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"315","DOI":"10.1101\/gr.258640.119","article-title":"Accurate and Complete Genomes from Metagenomes","volume":"30","author":"Chen","year":"2020","journal-title":"Genome Res."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"566","DOI":"10.1038\/nbt.4163","article-title":"KBase: The United States Department of Energy Systems Biology Knowledgebase","volume":"36","author":"Arkin","year":"2018","journal-title":"Nat. Biotechnol."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"D733","DOI":"10.1093\/nar\/gkv1189","article-title":"Reference Sequence (RefSeq) Database at NCBI: Current Status, Taxonomic Expansion, and Functional Annotation","volume":"44","author":"Wright","year":"2016","journal-title":"Nucleic Acids Res."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"468","DOI":"10.3389\/fmicb.2020.00468","article-title":"Analysis of 1000+ Type-Strain Genomes Substantially Improves Taxonomic Classification of Alphaproteobacteria","volume":"11","author":"Schleuning","year":"2020","journal-title":"Front. Microbiol."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"895","DOI":"10.1099\/ijsem.0.003276","article-title":"The Global Catalogue of Microorganisms (GCM) 10K Type Strain Sequencing Project: Providing Services to Taxonomists for Standard Genome Sequencing and Annotation","volume":"69","author":"Wu","year":"2019","journal-title":"Int. J. Syst. Evol. Microbiol."},{"key":"ref_36","doi-asserted-by":"crossref","unstructured":"Qu, L., Feng, X., Chen, Y., Li, L., Wang, X., Hu, Z., Wang, H., and Luo, H. (2021). Metapopulation Structure of Diatom-Associated Marine Bacteria. bioRxiv.","DOI":"10.1101\/2021.03.10.434754"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"1312","DOI":"10.1093\/bioinformatics\/btu033","article-title":"RAxML Version 8: A Tool for Phylogenetic Analysis and Post-Analysis of Large Phylogenies","volume":"30","author":"Stamatakis","year":"2014","journal-title":"Bioinformatics"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"1164","DOI":"10.1093\/bioinformatics\/btr088","article-title":"ProtTest 3: Fast Selection of Best-Fit Models of Protein Evolution","volume":"27","author":"Darriba","year":"2011","journal-title":"Bioinformatics"},{"key":"ref_39","doi-asserted-by":"crossref","unstructured":"Hyatt, D., Chen, G.-L., LoCascio, P.F., Land, M.L., Larimer, F.W., and Hauser, L.J. (2010). Prodigal: Prokaryotic Gene Recognition and Translation Initiation Site Identification. BMC Bioinform., 11.","DOI":"10.1186\/1471-2105-11-119"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"276","DOI":"10.1016\/S0168-9525(00)02024-2","article-title":"EMBOSS: The European Molecular Biology Open Software Suite","volume":"16","author":"Rice","year":"2000","journal-title":"Trends Genet."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"960","DOI":"10.1101\/gr.5578007","article-title":"A Framework for Collaborative Analysis of ENCODE Data: Making Large-Scale Analyses Biologist-Friendly","volume":"17","author":"Blankenberg","year":"2007","journal-title":"Genome Res."},{"key":"ref_42","doi-asserted-by":"crossref","unstructured":"Lechner, M., Findei\u00df, S., Steiner, L., Marz, M., Stadler, P.F., and Prohaska, S.J. (2011). Proteinortho: Detection of (Co-)Orthologs in Large-Scale Analysis. BMC Bioinform., 12.","DOI":"10.1186\/1471-2105-12-124"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"D457","DOI":"10.1093\/nar\/gkv1070","article-title":"KEGG as a Reference Resource for Gene and Protein Annotation","volume":"44","author":"Kanehisa","year":"2016","journal-title":"Nucleic Acids Res."},{"key":"ref_44","unstructured":"Krassowski, M. (2021, December 21). Krassowski\/Complex-Upset. Available online: https:\/\/zenodo.org\/record\/5762625."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"726","DOI":"10.1016\/j.jmb.2015.11.006","article-title":"BlastKOALA and GhostKOALA: KEGG Tools for Functional Characterization of Genome and Metagenome Sequences","volume":"428","author":"Kanehisa","year":"2016","journal-title":"J. Mol. Biol."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"D412","DOI":"10.1093\/nar\/gkaa913","article-title":"Pfam: The Protein Families Database in 2021","volume":"49","author":"Mistry","year":"2021","journal-title":"Nucleic Acids Res."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"1085","DOI":"10.1093\/bib\/bbx085","article-title":"The BioCyc Collection of Microbial Genomes and Metabolic Pathways","volume":"20","author":"Karp","year":"2019","journal-title":"Brief. Bioinform."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"311","DOI":"10.3109\/10408418709104443","article-title":"Mechanisms of Halotolerance in Microorganisms","volume":"14","author":"Vreeland","year":"2009","journal-title":"Crit. Rev. Microbiol."},{"key":"ref_49","doi-asserted-by":"crossref","unstructured":"Vera-Gargallo, B., and Ventosa, A. (2018). Metagenomic Insights into the Phylogenetic and Metabolic Diversity of the Prokaryotic Community Dwelling in Hypersaline Soils from the Odiel Saltmarshes (SW Spain). Genes, 9.","DOI":"10.3390\/genes9030152"},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"636","DOI":"10.1038\/ismej.2013.165","article-title":"Trehalose\/2-Sulfotrehalose Biosynthesis and Glycine-Betaine Uptake Are Widely Spread Mechanisms for Osmoadaptation in the Halobacteriales","volume":"8","author":"Youssef","year":"2014","journal-title":"ISME J."},{"key":"ref_51","doi-asserted-by":"crossref","unstructured":"Matarredona, L., Camacho, M., Zafrilla, B., Bonete, M.-J., and Esclapez, J. (2020). The Role of Stress Proteins in Haloarchaea and Their Adaptive Response to Environmental Shifts. Biomolecules, 10.","DOI":"10.3390\/biom10101390"},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"353","DOI":"10.1093\/femsre\/fuy009","article-title":"Strategies of Adaptation of Microorganisms of the Three Domains of Life to High Salt Concentrations","volume":"42","author":"Oren","year":"2018","journal-title":"FEMS Microbiol. Rev."},{"key":"ref_53","doi-asserted-by":"crossref","unstructured":"Becker, E.A., Seitzer, P.M., Tritt, A., Larsen, D., Krusor, M., Yao, A.I., Wu, D., Madern, D., Eisen, J.A., and Darling, A.E. (2014). Phylogenetically Driven Sequencing of Extremely Halophilic Archaea Reveals Strategies for Static and Dynamic Osmo-Response. PLOS Genet., 10.","DOI":"10.1371\/journal.pgen.1004784"},{"key":"ref_54","unstructured":"Kolde, R. (2021, December 21). pheatmap: Pretty Heatmaps. R Package Version 1.0.12. Available online: http:\/\/CRAN.R-project.org\/package=pheatmap."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"550","DOI":"10.1186\/s13059-014-0550-8","article-title":"Moderated Estimation of Fold Change and Dispersion for RNA-Seq Data with DESeq2","volume":"15","author":"Love","year":"2014","journal-title":"Genome Biol."},{"key":"ref_56","unstructured":"R Core Team (2020). R: A Language and Environment for Statistical Computing, R Foundation for Statistical Computing."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"2386","DOI":"10.1099\/ijsem.0.002809","article-title":"Using Average Nucleotide Identity to Improve Taxonomic Assignments in Prokaryotic Genomes at the NCBI","volume":"68","author":"Ciufo","year":"2018","journal-title":"Int. J. Syst. Evol. Microbiol."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"19126","DOI":"10.1073\/pnas.0906412106","article-title":"Shifting the Genomic Gold Standard for the Prokaryotic Species Definition","volume":"106","author":"Richter","year":"2009","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"346","DOI":"10.1099\/ijs.0.059774-0","article-title":"Towards a Taxonomic Coherence between Average Nucleotide Identity and 16S rRNA Gene Sequence Similarity for Species Demarcation of Prokaryotes","volume":"64","author":"Kim","year":"2014","journal-title":"Int. J. Syst. Evol. Microbiol."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"222","DOI":"10.1111\/j.1462-2920.2009.02062.x","article-title":"Widespread Known and Novel Phosphonate Utilization Pathways in Marine Bacteria Revealed by Functional Screening and Metagenomic Analyses","volume":"12","author":"Martinez","year":"2010","journal-title":"Environ. Microbiol."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"787","DOI":"10.1007\/s00792-012-0476-6","article-title":"Acid-Shifted Isoelectric Point Profiles of the Proteins in a Hypersaline Microbial Mat: An Adaptation to Life at High Salt Concentrations?","volume":"16","author":"Oren","year":"2012","journal-title":"Extremophiles"},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"5665","DOI":"10.1128\/AEM.71.10.5665-5677.2005","article-title":"Overview of the Marine Roseobacter Lineage","volume":"71","author":"Buchan","year":"2005","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"531","DOI":"10.1007\/s00203-008-0353-y","article-title":"Diversity, Ecology, and Genomics of the Roseobacter Clade: A Short Overview","volume":"189","author":"Brinkhoff","year":"2008","journal-title":"Arch. Microbiol."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"504","DOI":"10.1128\/MMBR.62.2.504-544.1998","article-title":"Biology of Moderately Halophilic Aerobic Bacteria","volume":"62","author":"Ventosa","year":"1998","journal-title":"Microbiol. Mol. Biol. Rev."},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"R70","DOI":"10.1186\/gb-2008-9-4-r70","article-title":"Molecular Signature of Hypersaline Adaptation: Insights from Genome and Proteome Composition of Halophilic Prokaryotes","volume":"9","author":"Paul","year":"2008","journal-title":"Genome Biol."},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"68","DOI":"10.1038\/nature04203","article-title":"Phosphonate Utilization by the Globally Important Marine Diazotroph Trichodesmium","volume":"439","author":"Dyhrman","year":"2006","journal-title":"Nature"}],"updated-by":[{"DOI":"10.3390\/genes13030523","type":"correction","label":"Correction","source":"publisher","updated":{"date-parts":[[2022,1,15]],"date-time":"2022-01-15T00:00:00Z","timestamp":1642204800000}}],"container-title":["Genes"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2073-4425\/13\/1\/148\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,8,3]],"date-time":"2025-08-03T22:32:22Z","timestamp":1754260342000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2073-4425\/13\/1\/148"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,1,15]]},"references-count":66,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2022,1]]}},"alternative-id":["genes13010148"],"URL":"https:\/\/doi.org\/10.3390\/genes13010148","relation":{"correction":[{"id-type":"doi","id":"10.3390\/genes13030523","asserted-by":"object"}]},"ISSN":["2073-4425"],"issn-type":[{"value":"2073-4425","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,1,15]]}}}