{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T02:57:23Z","timestamp":1760151443198,"version":"build-2065373602"},"reference-count":87,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2022,3,18]],"date-time":"2022-03-18T00:00:00Z","timestamp":1647561600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Genes"],"abstract":"<jats:p>The lack of an easy and fast radiation-exposure testing method with a dosimetric ability complicates triage and treatment in response to a nuclear detonation, radioactive material release, or clandestine exposure. The potential of transcriptomics in radiation diagnosis and prognosis were assessed here using wet skin (blood\/skin) biopsies obtained at hour 2 and days 4, 7, 21, and 28 from a mouse radiation model. Analysis of significantly differentially transcribed genes (SDTG; p \u2264 0.05 and FC \u2265 2) during the first post-exposure week identified the glycoprotein 6 (GP-VI) signaling, the dendritic cell maturation, and the intrinsic prothrombin activation pathways as the top modulated pathways with stable inactivation after lethal exposures (20 Gy) and intermittent activation after sublethal (1, 3, 6 Gy) exposure time points (TPs). Interestingly, these pathways were inactivated in the late TPs after sublethal exposure in concordance with a delayed deleterious effect. Modulated transcription of a variety of collagen types, laminin, and peptidase genes underlay the modulated functions of these hematologically important pathways. Several other SDTGs related to platelet and leukocyte development and functions were identified. These results outlined genetic determinants that were crucial to clinically documented radiation-induced hematological and skin damage with potential countermeasure applications.<\/jats:p>","DOI":"10.3390\/genes13030538","type":"journal-article","created":{"date-parts":[[2022,3,20]],"date-time":"2022-03-20T21:26:22Z","timestamp":1647811582000},"page":"538","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Transcriptomics of Wet Skin Biopsies Predict Early Radiation-Induced Hematological Damage in a Mouse Model"],"prefix":"10.3390","volume":"13","author":[{"given":"Abdulnaser","family":"Alkhalil","sequence":"first","affiliation":[{"name":"Firefighters\u2019 Burn and Surgical Research Laboratory, MedStar Health Research Institute, Washington, DC 20010, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John","family":"Clifford","sequence":"additional","affiliation":[{"name":"Pain and Sensory Trauma Care Research Team, US Army Institute of Surgical Research, Fort Sam Houston, San Antonio, TX 78234, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stacy Ann","family":"Miller","sequence":"additional","affiliation":[{"name":"Medical Readiness Systems Biology Branch, Center for Military Psychiatry and Neuroscience Research, Walter Reed Army Institute of Research, Silver Spring, MD 20910, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aarti","family":"Gautam","sequence":"additional","affiliation":[{"name":"Medical Readiness Systems Biology Branch, Center for Military Psychiatry and Neuroscience Research, Walter Reed Army Institute of Research, Silver Spring, MD 20910, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2899-0216","authenticated-orcid":false,"given":"Marti","family":"Jett","sequence":"additional","affiliation":[{"name":"Walter Reed Army Institute of Research, Silver Spring, MD 20910, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rasha","family":"Hammamieh","sequence":"additional","affiliation":[{"name":"Medical Readiness Systems Biology Branch, Center for Military Psychiatry and Neuroscience Research, Walter Reed Army Institute of Research, Silver Spring, MD 20910, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lauren T.","family":"Moffatt","sequence":"additional","affiliation":[{"name":"Firefighters\u2019 Burn and Surgical Research Laboratory, MedStar Health Research Institute, Washington, DC 20010, USA"},{"name":"Department of Biochemistry and Molecular Biology, Georgetown University School of Medicine, Washington, DC 20010, USA"},{"name":"Department of Surgery, Georgetown University School of Medicine, Washington, DC 20010, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jeffrey W.","family":"Shupp","sequence":"additional","affiliation":[{"name":"Firefighters\u2019 Burn and Surgical Research Laboratory, MedStar Health Research Institute, Washington, DC 20010, USA"},{"name":"Department of Biochemistry and Molecular Biology, Georgetown University School of Medicine, Washington, DC 20010, USA"},{"name":"Department of Surgery, Georgetown University School of Medicine, Washington, DC 20010, USA"},{"name":"The Burn Center, Department of Surgery, MedStar Washington Hospital Center, Washington, DC 20010, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2022,3,18]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1097\/01.HP.0000348466.09978.77","article-title":"Combined injury: Factors with potential to impact radiation dose assessments","volume":"98","author":"Ledney","year":"2010","journal-title":"Health Phys."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1375","DOI":"10.2106\/JBJS.18.01334","article-title":"An Estimation of Lifetime Fatal Carcinogenesis Risk Attributable to Radiation Exposure in the First Year Following Polytrauma: A Major Trauma Center\u2019s Experience over 10 Years","volume":"101","author":"Howard","year":"2019","journal-title":"JBJS"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"712","DOI":"10.1667\/RR1295.1","article-title":"Medical Countermeasures for Radiation Combined Injury: Radiation with Burn, Blast, Trauma and\/or Sepsis. 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