{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,26]],"date-time":"2026-03-26T21:12:01Z","timestamp":1774559521600,"version":"3.50.1"},"reference-count":25,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2022,4,25]],"date-time":"2022-04-25T00:00:00Z","timestamp":1650844800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Genes"],"abstract":"<jats:p>Glycosylphosphatidylinositol-anchored sperm hyaluronidases (HYAL) assist sperm penetration through the cumulus\u2013oocyte complex (COC), but their role in mammalian fertilization remains unclear. Previously, we demonstrated that sperm from HYAL 5 and 7 double-knockout (dKO) mice produced significantly less offspring than sperm from wild-type mice due to defective COC dispersal. However, the HYAL6 gene remained active in the sperm from the dKO mice, indicating that they were not entirely infertile. This study explored the role of HYAL6 in fertilization by analyzing HYAL6-mutant mice. In this mouse model, HYAL5 and HYAL7 were present in the HYAL6-knockout sperm, and they could disperse hyaluronic acid. We found that HYAL6 was present on the surface of sperm. However, male mice lacking the HYAL6 gene had normal fertility, testicular integrity, and sperm characteristics. Furthermore, in vitro fertilization assays demonstrated that HYAL6-deficient epididymal sperm functioned normally. Therefore, HYAL6 is dispensable for fertilization.<\/jats:p>","DOI":"10.3390\/genes13050753","type":"journal-article","created":{"date-parts":[[2022,4,25]],"date-time":"2022-04-25T21:16:28Z","timestamp":1650921388000},"page":"753","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Hyaluronidase 6 Does Not Affect Cumulus\u2013Oocyte Complex Dispersal and Male Mice Fertility"],"prefix":"10.3390","volume":"13","author":[{"given":"Hyewon","family":"Bang","sequence":"first","affiliation":[{"name":"College of Pharmacy, Catholic University of Daegu, Gyeongsan-si 38430, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sujin","family":"Lee","sequence":"additional","affiliation":[{"name":"College of Pharmacy, Catholic University of Daegu, Gyeongsan-si 38430, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8477-4358","authenticated-orcid":false,"given":"Pil-Soo","family":"Jeong","sequence":"additional","affiliation":[{"name":"Futuristic Animal Resource and Research Center, Korea Research Institute of Bioscience and Biotechnology, Cheongju-si 28116, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7400-9022","authenticated-orcid":false,"given":"Dong-Won","family":"Seol","sequence":"additional","affiliation":[{"name":"Preclinical Research Center, Daegu-Gyeongbuk Medical Innovation Foundation (DGMIF), Daegu 41061, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daeun","family":"Son","sequence":"additional","affiliation":[{"name":"College of Pharmacy, Catholic University of Daegu, Gyeongsan-si 38430, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Young-Hyun","family":"Kim","sequence":"additional","affiliation":[{"name":"National Primate Research Center (NPCR), Korea Research Institute of Bioscience and Biotechnology, Cheongju-si 28116, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0489-6719","authenticated-orcid":false,"given":"Bong-Seok","family":"Song","sequence":"additional","affiliation":[{"name":"National Primate Research Center (NPCR), Korea Research Institute of Bioscience and Biotechnology, Cheongju-si 28116, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4492-1085","authenticated-orcid":false,"given":"Bo-Woong","family":"Sim","sequence":"additional","affiliation":[{"name":"National Primate Research Center (NPCR), Korea Research Institute of Bioscience and Biotechnology, Cheongju-si 28116, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9901-719X","authenticated-orcid":false,"given":"Soojin","family":"Park","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology, Melvin & Bren Simon Comprehensive Cancer Center, Indiana University School of Medicine 635 Barnhill Drive, Indianapolis, IN 46202, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dong-Mok","family":"Lee","sequence":"additional","affiliation":[{"name":"Biomedical Manufacturing Technology Center, Korea Institute of Industrial Technology, Yeongcheon-si 38822, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0201-0416","authenticated-orcid":false,"given":"Gabbine","family":"Wee","sequence":"additional","affiliation":[{"name":"Preclinical Research Center, Daegu-Gyeongbuk Medical Innovation Foundation (DGMIF), Daegu 41061, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joon-Suk","family":"Park","sequence":"additional","affiliation":[{"name":"Preclinical Research Center, Daegu-Gyeongbuk Medical Innovation Foundation (DGMIF), Daegu 41061, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sun-Uk","family":"Kim","sequence":"additional","affiliation":[{"name":"Futuristic Animal Resource and Research Center, Korea Research Institute of Bioscience and Biotechnology, Cheongju-si 28116, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ekyune","family":"Kim","sequence":"additional","affiliation":[{"name":"College of Pharmacy, Catholic University of Daegu, Gyeongsan-si 38430, Korea"},{"name":"Biohealth Convergence Research center for East sea Rim, Catholic University of Daegu, Gyeongsan-si 38430, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2022,4,25]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"70","DOI":"10.1016\/0168-9525(89)90029-2","article-title":"The new mouse genetics: Altering the genome by gene targeting","volume":"5","author":"Capecchi","year":"1989","journal-title":"Trends Genet."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"154","DOI":"10.1038\/292154a0","article-title":"Establishment in culture of pluripotential cells from mouse embryos","volume":"292","author":"Evans","year":"1981","journal-title":"Nature"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"321","DOI":"10.1038\/nrg3686","article-title":"A guide to genome engineering with programmable nucleases","volume":"15","author":"Kim","year":"2014","journal-title":"Nat. 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