{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,8]],"date-time":"2026-03-08T22:10:12Z","timestamp":1773007812343,"version":"3.50.1"},"reference-count":53,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2022,5,29]],"date-time":"2022-05-29T00:00:00Z","timestamp":1653782400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Waseda University Grant for Special Research Projects","award":["2020C-375"],"award-info":[{"award-number":["2020C-375"]}]},{"name":"Waseda University Grant for Special Research Projects","award":["17K08621"],"award-info":[{"award-number":["17K08621"]}]},{"name":"Waseda University Grant for Special Research Projects","award":["20H03408"],"award-info":[{"award-number":["20H03408"]}]},{"name":"Waseda University Advanced Research Center for Human Sciences, MEXT JSPS KAKENHI","award":["2020C-375"],"award-info":[{"award-number":["2020C-375"]}]},{"name":"Waseda University Advanced Research Center for Human Sciences, MEXT JSPS KAKENHI","award":["17K08621"],"award-info":[{"award-number":["17K08621"]}]},{"name":"Waseda University Advanced Research Center for Human Sciences, MEXT JSPS KAKENHI","award":["20H03408"],"award-info":[{"award-number":["20H03408"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Genes"],"abstract":"<jats:p>Ultraviolet A (UVA) radiation can pass through the epidermis and reach the dermal skin layer, contributing to photoaging, DNA damage, and photocarcinogenesis in dermal fibroblasts. High-dose UVA exposure induces erythema, whereas low-dose, long-term UVA exposure causes skin damage and cell senescence. Biomarkers for evaluating damage caused by low-dose UVA in fibroblasts are lacking, making it difficult to develop therapeutic agents for skin aging and aging-associated diseases. We performed RNA-sequencing to investigate gene and pathway alterations in low-dose UVA-irradiated human skin-derived NB1RGB primary fibroblasts. Differentially expressed genes were identified and subjected to Gene Ontology and reactome pathway analysis, which revealed enrichment in genes in the senescence-associated secretory phenotype, apoptosis, respiratory electron transport, and transcriptional regulation by tumor suppressor p53 pathways. Insulin-like growth factor binding protein 7 (IGFBP7) showed the lowest p-value in RNA-sequencing analysis and was associated with the senescence-associated secretory phenotype. Protein\u2013protein interaction analysis revealed that Fos proto-oncogene had a high-confidence network with IGFBP7 as transcription factor of the IGFBP7 gene among SASP hit genes, which were validated using RT-qPCR. Because of their high sensitivity to low-dose UVA radiation, Fos and IGFBP7 show potential as biomarkers for evaluating the effect of low-dose UVA radiation on dermal fibroblasts.<\/jats:p>","DOI":"10.3390\/genes13060974","type":"journal-article","created":{"date-parts":[[2022,5,29]],"date-time":"2022-05-29T08:50:51Z","timestamp":1653814251000},"page":"974","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Genome-Wide RNA Sequencing Analysis in Human Dermal Fibroblasts Exposed to Low-Dose Ultraviolet A Radiation"],"prefix":"10.3390","volume":"13","author":[{"given":"Jinyun","family":"Wang","sequence":"first","affiliation":[{"name":"Laboratory of Food and Life Science, Faculty of Human Sciences, Waseda University, Tokorozawa 359-1192, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Satoshi","family":"Yano","sequence":"additional","affiliation":[{"name":"Laboratory of Food and Life Science, Faculty of Human Sciences, Waseda University, Tokorozawa 359-1192, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kun","family":"Xie","sequence":"additional","affiliation":[{"name":"Laboratory of Food and Life Science, Faculty of Human Sciences, Waseda University, Tokorozawa 359-1192, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yoshihisa","family":"Ohata","sequence":"additional","affiliation":[{"name":"Laboratory of Food and Life Science, Faculty of Human Sciences, Waseda University, Tokorozawa 359-1192, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4722-6336","authenticated-orcid":false,"given":"Taichi","family":"Hara","sequence":"additional","affiliation":[{"name":"Laboratory of Food and Life Science, Faculty of Human Sciences, Waseda University, Tokorozawa 359-1192, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2022,5,29]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"425","DOI":"10.1562\/2006-11-14-RA-1081","article-title":"Radiometric quantities and units used in photobiology and photochemistry: Recommendations of the Commission Internationale de L\u2019Eclairage (International Commission on Illumination)","volume":"83","author":"Sliney","year":"2007","journal-title":"Photochem. 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