{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,5]],"date-time":"2026-06-05T06:13:12Z","timestamp":1780639992809,"version":"3.54.1"},"reference-count":59,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2022,6,2]],"date-time":"2022-06-02T00:00:00Z","timestamp":1654128000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31201655"],"award-info":[{"award-number":["31201655"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["2014PY064"],"award-info":[{"award-number":["2014PY064"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Fundamental Research Fund for the Central Universities","award":["31201655"],"award-info":[{"award-number":["31201655"]}]},{"name":"Fundamental Research Fund for the Central Universities","award":["2014PY064"],"award-info":[{"award-number":["2014PY064"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Genes"],"abstract":"<jats:p>Powdery mildew disease caused by Podosphaerapannosa is the most widespread disease in global cut-rose production, as well as a major disease in garden and pot roses. In this study, the powdery mildew resistance of different wild rose varieties was evaluated. Rose varieties with high resistance and high sensitivity were used for cytological observation and transcriptome and expression profile analyses to study changes at the morphological and molecular levels during the interaction between Rosa multiflora and P. pannosa. There were significant differences in powdery mildew resistance among three R. multiflora plants; R. multiflora \u201813\u2019 had high resistance, while R. multiflora \u20184\u2019 and \u20181\u2019 had high susceptibility. Cytological observations showed that in susceptible plants, 96 and 144 h after inoculation, hyphae were observed in infected leaves; hyphae infected the leaf tissue through the stoma of the lower epidermis, while papillae were formed on the upper epidermis of susceptible leaf tissue. Gene ontology enrichment analysis showed that the differentially expressed genes that were significantly enriched in biological process functions were related to the secondary metabolic process, the most significantly enriched cellular component function was cell wall, and the most significantly enriched molecular function was chitin binding. Changes in the transcript levels of important defense-related genes were analyzed. The results showed that chitinase may have played an important role in the interactions between resistant R. multiflora and P. pannosa. Jasmonic acid and ethylene (JA\/ET) signaling pathways might be triggered in the interaction between susceptible R. multiflora and P. pannosa. In the resistant R. multiflora, the salicylic acid (SA) signaling pathway was induced earlier. Between susceptible plants and resistant plants, key phenylpropanoid pathway genes were induced and upregulated after P. pannosa inoculation, demonstrating that the phenylpropanoid pathway and secondary metabolites may play important and active roles in R. multiflora defense against powdery mildew infection.<\/jats:p>","DOI":"10.3390\/genes13061003","type":"journal-article","created":{"date-parts":[[2022,6,2]],"date-time":"2022-06-02T04:46:23Z","timestamp":1654145183000},"page":"1003","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Morphological and Molecular Analyses of the Interaction between Rosa multiflora and Podosphaera pannosa"],"prefix":"10.3390","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2138-3541","authenticated-orcid":false,"given":"Ying","family":"Bao","sequence":"first","affiliation":[{"name":"Key Laboratory of Horticultural Plant Biology of Ministry of Education, College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan 430070, China"},{"name":"Department of Life Science, Tangshan Normal University, Tangshan 063000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xue","family":"Zhang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Horticultural Plant Biology of Ministry of Education, College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan 430070, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoxiang","family":"Sun","sequence":"additional","affiliation":[{"name":"Key Laboratory of Horticultural Plant Biology of Ministry of Education, College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan 430070, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manzhu","family":"Bao","sequence":"additional","affiliation":[{"name":"Key Laboratory of Horticultural Plant Biology of Ministry of Education, College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan 430070, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuanyuan","family":"Wang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Horticultural Plant Biology of Ministry of Education, College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan 430070, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,6,2]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Roberts, A., Debener, T., and Gudin, S. (2003). Pot rose production. Encyclopedia of Rose Science, Elsevier Science.","DOI":"10.1016\/B0-12-227620-5\/90006-5"},{"key":"ref_2","unstructured":"Spencer, D.M. (1978). The Powdery Mildews, Academic Press."},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Noack, R. (2003). Breeding\/selection strategies for disease and pest resistance. Encyclopedia of Rose Science, Elsevier.","DOI":"10.1016\/B0-12-227620-5\/00100-2"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1016\/j.scienta.2004.08.002","article-title":"Effect of air humidity variation on powdery mildew and keeping quality of cut roses","volume":"104","author":"Mortensen","year":"2005","journal-title":"Sci. Hortic."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"193","DOI":"10.17660\/ActaHortic.2005.690.29","article-title":"A review of biological control of rose powdery mildew (Spaerotheca pannosa var. rosae) by fungal antagonists","volume":"690","author":"Eken","year":"2005","journal-title":"Acta Hortic."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1111\/j.1439-0434.2005.01053.x","article-title":"Podosphaera pannosa (syn. Sphaerotheca pannosa) on Rosa and Prunus spp.: Characterization of pathotypes by differential plant reactions and ITS sequences","volume":"154","author":"Leus","year":"2006","journal-title":"J. Phytopathol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1016\/j.pestbp.2016.07.003","article-title":"Advances and prospects in biogenic substances against plant virus: A review","volume":"135","author":"Zhao","year":"2017","journal-title":"Pestic. Biochem. Physiol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"256","DOI":"10.1007\/s00122-003-1240-1","article-title":"Isolation and identification of eight races of powdery mildew of roses (Podosphaera pannosa) (Wallr.: Fr.) de Bary and the genetic analysis of the resistance gene Rpp1","volume":"107","author":"Linde","year":"2003","journal-title":"Theor. Appl. Genet."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"101","DOI":"10.1146\/annurev.phyto.45.062806.094325","article-title":"Cell wall-associated mechanisms of disease resistance and susceptibility","volume":"45","author":"Huckelhoven","year":"2007","journal-title":"Annu. Rev. Phytopathol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"299","DOI":"10.1007\/s11103-018-00818-2","article-title":"Interaction of roses with a biotrophic and a hemibiotrophic leaf pathogen leads to differences in defense transcriptome activation","volume":"99","author":"Neu","year":"2019","journal-title":"Plant Mol. Biol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"26","DOI":"10.1016\/j.tplants.2003.11.008","article-title":"How plants communicate using the underground information superhighway","volume":"9","author":"Bais","year":"2004","journal-title":"Trends Plant Sci."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"1085","DOI":"10.2307\/3870059","article-title":"Stress-induced phenylpropanoid metabolism","volume":"7","author":"Dixon","year":"1995","journal-title":"Plant Cell"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"371","DOI":"10.1046\/j.1364-3703.2002.00131.x","article-title":"The phenylpropanoid pathway and plant defence: A genomics perspective","volume":"3","author":"Dixon","year":"2002","journal-title":"Mol. Plant Pathol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"399","DOI":"10.1016\/j.mib.2005.06.008","article-title":"The arms race continues: Battle strategies between plants and fungal pathogens","volume":"8","author":"Maor","year":"2005","journal-title":"Curr. Opin. Microbiol."},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"Ahmad, A., Niwa, Y., Goto, S., Ogawa, T., Shimizu, M., Suzuki, A., Kobayashi, K., and Kobayashi, H. (2015). bHLH106 integrates functions of multiple genes through their G-Box to confer salt tolerance on Arabidopsis. PLoS ONE, 10.","DOI":"10.1371\/journal.pone.0126872"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"215","DOI":"10.1016\/0092-8674(89)90894-5","article-title":"Signals and transduction mechanisms for activation of plant defenses against microbial attack","volume":"56","author":"Lamb","year":"1989","journal-title":"Cell"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"288","DOI":"10.1016\/S1049-9644(02)00108-1","article-title":"The role of salicylic acid in induced systemic resistance elicited by plant growth-promoting rhizobacteria against blue mold of tobacco","volume":"25","author":"Zhang","year":"2002","journal-title":"Biol. Control"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1146\/annurev.phyto.44.070505.143425","article-title":"Significance of inducible defense-related proteins in infected plants","volume":"44","author":"Rep","year":"2006","journal-title":"Annu. Rev. Phytopathol."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"839","DOI":"10.1146\/annurev-arplant-042811-105606","article-title":"Systemic acquired resistance: Turning local infection into global defense","volume":"64","author":"Fu","year":"2013","journal-title":"Annu. Rev. Plant Biol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"9166","DOI":"10.1073\/pnas.1511182112","article-title":"Spatial and temporal regulation of biosynthesis of the plant immune signal salicylic acid","volume":"112","author":"Zheng","year":"2015","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"85","DOI":"10.1006\/pmpp.1999.0213","article-title":"The families of pathogenesis-related proteins, their activities, and comparative analysis of PR-1 type proteins","volume":"55","year":"1999","journal-title":"Physiol. Mol. Plant Pathol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"1619","DOI":"10.1094\/MPMI-11-09-0271","article-title":"Morphological and molecular analyses of host and nonhost interactions involving barley and wheat and the covered smut pathogen Ustiago hordei","volume":"23","author":"Gaudet","year":"2010","journal-title":"Mol. Plant-Microbe Interact."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"607","DOI":"10.1007\/s11103-018-0718-9","article-title":"TaEDS1 genes positively regulate resistance to powdery mildew in wheat","volume":"96","author":"Chen","year":"2018","journal-title":"Plant Mol. Biol."},{"key":"ref_24","first-page":"823","article-title":"A study on JA-and BTH-induced resistance of Rosa rugosa \u2018Plena\u2019 to powdery mildew (Sphaerotheca pannosa)","volume":"29","author":"Yan","year":"2018","journal-title":"J. Foraminifer. Res."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"315","DOI":"10.1248\/bpb.29.315","article-title":"Identification of medicinal atractylodes based on ITS sequences of nrDNA","volume":"29","author":"Mao","year":"2006","journal-title":"Biol. Pharm. Bull."},{"key":"ref_26","unstructured":"Li, H.P. (2009). Plant Microscopy, Science Press."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"153","DOI":"10.1105\/tpc.000679","article-title":"The Jasmonate signal pathway","volume":"14","author":"Turner","year":"2002","journal-title":"Plant Cell"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"342","DOI":"10.1016\/j.pbi.2007.05.001","article-title":"Cytoskeleton and cell wall function in penetration resistance","volume":"10","author":"Hardham","year":"2007","journal-title":"Curr. Opin. Plant Biol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"454","DOI":"10.1111\/pbi.13728","article-title":"Thinopyrum intermedium TiAP1 interacts with a chitin deacetylase from Blumeria graminis f. sp. tritici and increases the resistance to Bgt in wheat","volume":"20","author":"Yang","year":"2022","journal-title":"Plant Biotechnol. J."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"8577","DOI":"10.1073\/pnas.1431173100","article-title":"A Pseudomonas syringae type III effector suppresses cell wall-based extracellular defense in susceptible Arabidopsis plants","volume":"100","author":"Hauck","year":"2003","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"655","DOI":"10.1094\/MPMI-22-6-0655","article-title":"The Xanthomonas campestris pv. vesicatoria type III effector protein XopJ inhibits protein secretion: Evidence for interference with cell wall-associated defense responses","volume":"22","author":"Bartetzko","year":"2009","journal-title":"Mol. Plant-Microbe Interact."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"279","DOI":"10.1007\/s11104-011-0916-6","article-title":"Protection of strawberry plants (Fragaria ananassa Duch.) against anthracnose disease induced by Azospirillum brasilense","volume":"356","author":"Tortora","year":"2012","journal-title":"Plant Soil"},{"key":"ref_33","doi-asserted-by":"crossref","unstructured":"Yang, C.C., Wu, P.F., Yao, X.H., Sheng, Y., Zhang, C.C., Lin, P., and Wang, K.L. (2022). Integrated Transcriptome and Metabolome Analysis Reveals Key Metabolites Involved in Camellia oleifera Defense against Anthracnose. Int. J. Mol. Sci., 23.","DOI":"10.3390\/ijms23010536"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"2194","DOI":"10.1104\/pp.111.185215","article-title":"Silicon-Induced Changes in Antifungal Phenolic Acids, Flavonoids and Key Phenylpropanoid Pathway Genes during the Interaction between Miniature Roses and the Biotrophic Pathogen Podosphaera pannosa","volume":"157","author":"Shetty","year":"2011","journal-title":"Plant Physiol."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"120","DOI":"10.1111\/j.1365-3059.2011.02493.x","article-title":"Silicon induced resistance against powdery mildew of roses caused by Podosphaera pannosa","volume":"61","author":"Shetty","year":"2012","journal-title":"Plant Pathol."},{"key":"ref_36","first-page":"34","article-title":"Relationship between phenylalanine ammonia-Lysae (PAL) activity and resistance to rice blast","volume":"6","author":"Zhang","year":"1987","journal-title":"Plant Physiol. Commun."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"389","DOI":"10.1007\/BF00014913","article-title":"Plant gene expression in response to pathogens","volume":"9","author":"Collinge","year":"1987","journal-title":"Plant Mol. Biol."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"259","DOI":"10.1146\/annurev.py.18.090180.001355","article-title":"Lignifigation as a mechanism of disease resistance","volume":"18","author":"Vance","year":"1980","journal-title":"Annu. Rev. Phytopathol."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"922","DOI":"10.1016\/j.cub.2007.05.018","article-title":"A receptor-like kinase mediates the response of Arabidopsis cells to the inhibition of cellulose synthesis","volume":"17","author":"Hematy","year":"2007","journal-title":"Curr. Biol."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"509","DOI":"10.1093\/jxb\/ern290","article-title":"Gene expression profiling and silencing reveal that monolignol biosynthesis plays a critical role in penetration defence in wheat against powdery mildew invasion","volume":"60","author":"Bhuiyan","year":"2009","journal-title":"J. Exp. Bot."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"627","DOI":"10.1111\/j.1469-8137.2010.03621.x","article-title":"Selective lignin downregulation leads to constitutive defense response expression in alfalfa (Medicago sativa L.)","volume":"90","author":"Gallego","year":"2011","journal-title":"New Phytol."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"5546","DOI":"10.1073\/pnas.85.15.5546","article-title":"Cinnamyl-alcohol dehydrogenase, a molecular marker specific for lignin synthesis: cDNA cloning and mRNA induction by fungal elicitor","volume":"85","author":"Walter","year":"1988","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1111\/j.1364-3703.2009.00578.x","article-title":"Cinnamyl alcohol dehydrogenases-C and D, key enzymes in lignin biosynthesis, play an essential role in disease resistance in Arabidopsis","volume":"11","author":"Tronchet","year":"2010","journal-title":"Mol. Plant Pathol."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"501","DOI":"10.1016\/j.proche.2015.03.067","article-title":"Peroxidase enzyme activity of rhizobacteria-introduced shallots bulbs to induce resistance of shallot towards bacterial leaf blight (Xanthomonas axonopodis pv. allii)","volume":"14","author":"Yanti","year":"2015","journal-title":"Prog. Chem."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"633","DOI":"10.1111\/j.1365-313X.2004.02236.x","article-title":"Host and non-host pathogens elicit different jasmonate\/ethylene responses in Arabidopsis","volume":"40","author":"Laurent","year":"2004","journal-title":"Plant J."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"65","DOI":"10.1111\/j.1365-3059.2004.00947.x","article-title":"The salicylic acid-dependent defence pathway is effective against different pathogens in tomato and tobacco","volume":"53","author":"Achuo","year":"2004","journal-title":"Plant Pathol."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"754","DOI":"10.1126\/science.261.5122.754","article-title":"Requirement of salicylic acid for the induction of systemic acquired resistance","volume":"261","author":"Gaffney","year":"1993","journal-title":"Science"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1046\/j.1364-3703.2002.00105.x","article-title":"The molecular characterization of two barley proteins establishes the novel PR-17 family of pathogenesis-related proteins","volume":"3","author":"Christensen","year":"2002","journal-title":"Mol. Plant Pathol."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"185","DOI":"10.1146\/annurev.phyto.42.040803.140421","article-title":"Systemic acquired resistance","volume":"42","author":"Durrant","year":"2004","journal-title":"Annu. Rev. Phytopathol."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"1347","DOI":"10.1104\/pp.107.100842","article-title":"Phosphatidylinositol 4-kinase activation is an early response to salicylic acid in Arabidopsis suspension cells","volume":"144","author":"Krinke","year":"2007","journal-title":"Plant Physiol."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"923","DOI":"10.1007\/s10658-017-1333-3","article-title":"Salicylic acid mediated multi-pronged strategy to combat bacterial blight disease (Xanthomonas axonopodis pv. punicae) in pomegranate","volume":"150","author":"Maity","year":"2018","journal-title":"Eur. J. Plant Pathol."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"731","DOI":"10.1105\/tpc.105.038372","article-title":"Arabidopsis PEN3\/PDR8, an ATP binding cassette transporter, contributes to non-host resistance to inappropriate pathogens that enter by direct penetration","volume":"18","author":"Stein","year":"2006","journal-title":"Plant Cell"},{"key":"ref_53","first-page":"586","article-title":"A subset of hypersensitive response marker genes, including HSR203J, is the downstream target of a spermine signal transduction pathway in tobacco","volume":"46","author":"Takahashi","year":"2010","journal-title":"Plant J."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"e0156","DOI":"10.1199\/tab.0156","article-title":"Salicylic acid biosynthesis and metabolism","volume":"9","author":"Dempsey","year":"2011","journal-title":"Arab. Book"},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"43991","DOI":"10.1038\/srep43991","article-title":"Elicitation of resistance and associated defense responses in Trichoderma hamatum induced protection against pearl millet downy mildew pathogen","volume":"7","author":"Siddaiah","year":"2017","journal-title":"Sci. Rep."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"1919","DOI":"10.1105\/tpc.003368","article-title":"The SCFcol1 Ubiquitin-Lligase complexes are required for Jasmonate response in Arabidopsis","volume":"14","author":"Xu","year":"2002","journal-title":"Plant Cell"},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"1217","DOI":"10.1007\/s00425-018-2860-7","article-title":"Induction of systemic resistance in tomato against Botrytis cinerea by N-decanoyl-homoserine lactone via jasmonic acid signaling","volume":"247","author":"Hu","year":"2018","journal-title":"Planta"},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"760","DOI":"10.1105\/tpc.009159","article-title":"NPR1 modulates cross-talk between salicylate-and jasmonate-dependent defense pathways through a novel function in the cytosol","volume":"15","author":"Spoel","year":"2003","journal-title":"Plant Cell"},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"15107","DOI":"10.1073\/pnas.95.25.15107","article-title":"Separate jasmonate-dependent and salicylate-dependent defense-response pathways in Arabidopsis are essential for resistance to distinct microbial pathogens","volume":"95","author":"Thomma","year":"1998","journal-title":"Proc. Natl. Acad. Sci. USA"}],"container-title":["Genes"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2073-4425\/13\/6\/1003\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T23:23:42Z","timestamp":1760138622000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2073-4425\/13\/6\/1003"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,6,2]]},"references-count":59,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2022,6]]}},"alternative-id":["genes13061003"],"URL":"https:\/\/doi.org\/10.3390\/genes13061003","relation":{},"ISSN":["2073-4425"],"issn-type":[{"value":"2073-4425","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,6,2]]}}}