{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,15]],"date-time":"2026-01-15T02:27:51Z","timestamp":1768444071309,"version":"3.49.0"},"reference-count":33,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2022,6,17]],"date-time":"2022-06-17T00:00:00Z","timestamp":1655424000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Taif University","award":["TURSP-2020\/153"],"award-info":[{"award-number":["TURSP-2020\/153"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Genes"],"abstract":"<jats:p>The Dombrock (DO) blood group system has two primary antigens, Doa and Dob, which can cause delayed hemolytic transfusion reactions. The paucity of specific monospecific antibodies can hamper the typing based on these antigens. Thus, blood group genotyping (BGG) was investigated as a possible solution. Sequence-specific primers were designed to target a single nucleotide polymorphism (rs11276) on the ART4 gene encoding the DO*A and DO*B alleles. Blood samples (n = 150) from randomly selected volunteer donors were used. DNA was extracted and resulting PCR products were purified and sequenced. The allelic frequencies of DO*A and DO*B were (n = 122, 40.67%) and (n = 178, 59.33%), respectively. The distributions of DO genotypes were as follows: DO*A\/DO*A (n = 20), 13.33%; DO*B\/DO*B (n = 48), 32.00%; and DO*A\/DO*B (n = 82), 54.67%. In conclusion, this study reports on the allelic frequencies of DO*A and DO*B of the DO blood group system in Jazan Province, Kingdom of Saudi Arabia. Furthermore, this study reports on the prevalence of each genotype, of which DO*A\/DO*B was the most abundant. This study contributes significantly to build the current blood donor database in Southwestern Saudi Arabia. Moreover, it may assist in providing safe blood to polytransfused patients and reduce the risk of the red cell alloimmunization.<\/jats:p>","DOI":"10.3390\/genes13061079","type":"journal-article","created":{"date-parts":[[2022,6,17]],"date-time":"2022-06-17T01:48:12Z","timestamp":1655430492000},"page":"1079","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Investigation of Dombrock Blood Group Alleles and Genotypes among Saudi Blood Donors in Southwestern Saudi Arabia"],"prefix":"10.3390","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1121-6256","authenticated-orcid":false,"given":"Amr J.","family":"Halawani","sequence":"first","affiliation":[{"name":"Department of Laboratory Medicine, Faculty of Applied Medical Sciences, Umm Al-Qura University, Makkah 24381, Saudi Arabia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Abdullah S.","family":"Mansor","sequence":"additional","affiliation":[{"name":"Department of Medical Laboratory Technology, Faculty of Applied Medical Sciences, Jazan University, Jazan 82911, Saudi Arabia"},{"name":"SMIRES for Consultation in Specialized Medical Laboratories, Jazan University, Jazan 82911, Saudi Arabia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5563-9491","authenticated-orcid":false,"given":"Hamza M.","family":"Assaggaf","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, Faculty of Applied Medical Sciences, Umm Al-Qura University, Makkah 24381, Saudi Arabia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1874-3422","authenticated-orcid":false,"given":"Hibah A.","family":"Almasmoum","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, Faculty of Applied Medical Sciences, Umm Al-Qura University, Makkah 24381, Saudi Arabia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6351-5034","authenticated-orcid":false,"given":"Hisham I.","family":"Abu-Tawil","sequence":"additional","affiliation":[{"name":"Department of Laboratory and Blood Bank, Prince Mohammed bin Nasser Hospital, Ministry of Health, Jazan 82943, Saudi Arabia"},{"name":"Department of Laboratory and Blood Bank, King Faisal Medical City for Southern Regions, Ministry of Health, Abha 62523, Saudi Arabia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8156-2069","authenticated-orcid":false,"given":"Khalaf F.","family":"Alsharif","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory Science, College of Applied Medical Sciences, Taif University, P.O. Box 11099, Taif 21944, Saudi Arabia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9225-1709","authenticated-orcid":false,"given":"Gasim","family":"Dobie","sequence":"additional","affiliation":[{"name":"Department of Medical Laboratory Technology, Faculty of Applied Medical Sciences, Jazan University, Jazan 82911, Saudi Arabia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4155-3379","authenticated-orcid":false,"given":"Mahmoud M.","family":"Habibullah","sequence":"additional","affiliation":[{"name":"Department of Medical Laboratory Technology, Faculty of Applied Medical Sciences, Jazan University, Jazan 82911, Saudi Arabia"},{"name":"SMIRES for Consultation in Specialized Medical Laboratories, Jazan University, Jazan 82911, Saudi Arabia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2022,6,17]]},"reference":[{"key":"ref_1","unstructured":"International Society of Blood Transfusion (2022, March 26). Red Cell Immunogenetics and Blood Group Terminology. Available online: http:\/\/www.isbtweb.org."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"313","DOI":"10.1038\/206313a0","article-title":"\u201cNew\u201d blood group antigen, Doa","volume":"206","author":"Swanson","year":"1965","journal-title":"Nature"},{"key":"ref_3","first-page":"382","article-title":"Enlargement of the Dombrock blood group system: The finding of anti-Dob","volume":"24","author":"Molthan","year":"1973","journal-title":"Vox Sang."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"71","DOI":"10.21307\/immunohematology-2019-206","article-title":"The Dombrock blood group system: A review","volume":"26","author":"Reid","year":"2010","journal-title":"Immunohematology"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"304","DOI":"10.1111\/j.1537-2995.1967.tb05522.x","article-title":"A new public antigenic determinant Gya (Gregory)","volume":"7","author":"Swanson","year":"1967","journal-title":"Transfusion"},{"key":"ref_6","first-page":"386","article-title":"New antibodies to high incidence antigenic determinants (anti-so anti-el anti-HY and anti-DP)","volume":"7","author":"Schmidt","year":"1967","journal-title":"Transfusion"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"322","DOI":"10.1111\/j.1537-2995.1972.tb04452.x","article-title":"Anti-Joa: An antibody defining a high-frequency erythrocyte antigen","volume":"12","author":"Jensen","year":"1972","journal-title":"Transfusion"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"177","DOI":"10.1111\/j.1423-0410.1995.tb03923.x","article-title":"Evidence that the Gya, Hy and Joa antigens belong to the Dombrock blood group system","volume":"68","author":"Banks","year":"1995","journal-title":"Vox Sang."},{"key":"ref_9","first-page":"53","article-title":"Evidence that the human blood group antigens Gya and Hy are carried on a novel glycosylphosphatidylinositol-linked erythrocyte membrane glycoprotein","volume":"60","author":"Spring","year":"1991","journal-title":"Vox Sang."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"1404","DOI":"10.1182\/blood.V75.7.1404.1404","article-title":"Evidence that several high-frequency human blood group antigens reside on phosphatidylinositol-linked erythrocyte membrane proteins","volume":"75","author":"Telen","year":"1990","journal-title":"Blood"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"1814","DOI":"10.1182\/blood-2018-11-833954","article-title":"Blood group genotyping","volume":"133","author":"Westhoff","year":"2019","journal-title":"Blood"},{"key":"ref_12","first-page":"545","article-title":"Acute hemolytic transfusion reaction due to anti-Dombrocka (Doa)","volume":"26","author":"Kruskall","year":"1986","journal-title":"Transfusion"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"477","DOI":"10.1046\/j.1537-2995.1991.31591263209.x","article-title":"Multiple hemolytic transfusion reactions caused by anti-Doa","volume":"31","author":"Judd","year":"1991","journal-title":"Transfusion"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"44","DOI":"10.1046\/j.1537-2995.1985.25185116501.x","article-title":"Anti-Dob implicated as the cause of a delayed hemolytic transfusion reaction","volume":"25","author":"Moheng","year":"1985","journal-title":"Transfusion"},{"key":"ref_15","first-page":"206","article-title":"The first reported case of anti-Dob causing an acute hemolytic transfusion reaction","volume":"66","author":"Halverson","year":"1994","journal-title":"Vox Sang."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"103","DOI":"10.21307\/immunohematology-2018-017","article-title":"Clinical significance of antibodies to antigens in the Scianna, Dombrock, Colton, Landsteiner-Weiner, Chido\/Rodgers, H, Kx, Cromer, Gerbich, knops, Indian, and ok blood group systems","volume":"34","author":"Crottet","year":"2018","journal-title":"Immunohematology"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"2621","DOI":"10.1182\/blood.V96.7.2621","article-title":"Identification of the Dombrock blood group glycoprotein as a polymorphic member of the ADP-ribosyltransferase gene family","volume":"96","author":"Gubin","year":"2000","journal-title":"Blood"},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"The 1000 Genomes Project Consortium (2015). A global reference for human genetic variation. Nature, 526, 68\u201374.","DOI":"10.1038\/nature15393"},{"key":"ref_19","first-page":"294","article-title":"Doa (Dombrock) blood group antigen in the Japanese","volume":"38","author":"Nakajima","year":"1980","journal-title":"Vox Sang."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"269","DOI":"10.1111\/j.1537-2995.1967.tb05516.x","article-title":"Blood groups of the Thais","volume":"7","author":"Chandanayingyong","year":"1967","journal-title":"Transfusion"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"3755","DOI":"10.1111\/trf.15572","article-title":"DO\/ART4 gene sequencing in sub-Saharan cohorts and African migrants: Useful data describing the diversity and spreading of rare variants","volume":"59","author":"Movia","year":"2019","journal-title":"Transfusion"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"S41","DOI":"10.1016\/j.jegh.2017.12.001","article-title":"Distribution of hemoglobinopathy disorders in Saudi Arabia based on data from the premarital screening and genetic counseling program, 2011\u20132015","volume":"7","author":"Alsaeed","year":"2018","journal-title":"J. Epidemiol. Glob. Health"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"4093","DOI":"10.2147\/IJGM.S360320","article-title":"Red Cell Alloimmunization and Autoimmunization Among Sickle Cell Disease and Thalassemia Patients in Jazan Province, Saudi Arabia","volume":"15","author":"Halawani","year":"2022","journal-title":"Int. J. Gen. Med."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"344","DOI":"10.7754\/Clin.Lab.2020.200633","article-title":"ABO, RH, and KEL1 antigens, phenotypes and haplotypes in Southwestern Saudi Arabia","volume":"67","author":"Halawani","year":"2021","journal-title":"Clin. Lab."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"173","DOI":"10.7754\/Clin.Lab.2020.200505","article-title":"Prevalence of Duffy blood group antigens and phenotypes among Saudi blood donors in Southwestern Saudi Arabia","volume":"67","author":"Halawani","year":"2021","journal-title":"Clin. Lab."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"9315","DOI":"10.2147\/IJGM.S344826","article-title":"Frequencies of MNS blood group antigens and phenotypes in Southwestern Saudi Arabia","volume":"14","author":"Halawani","year":"2021","journal-title":"Int. J. Gen. Med."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"251","DOI":"10.1016\/j.sjbs.2021.08.081","article-title":"The frequencies of Kidd blood group antigens and phenotypes among Saudi blood donors in Southwestern Saudi Arabia","volume":"29","author":"Halawani","year":"2022","journal-title":"Saudi J. Biol. Sci."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"92S","DOI":"10.1111\/j.1537-2995.2005.00527.x","article-title":"Complexities of the Dombrock blood group system revealed","volume":"45","author":"Reid","year":"2005","journal-title":"Transfusion"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"528","DOI":"10.1182\/blood-2011-11-327361","article-title":"Red blood cell alloimmunization in sickle cell disease: Pathophysiology, risk factors, and transfusion management","volume":"120","author":"Yazdanbakhsh","year":"2012","journal-title":"Blood"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"91","DOI":"10.1111\/j.1423-0410.2002.tb05275.x","article-title":"DNA analysis to find rare blood donors when antisera is not available","volume":"83","author":"Reid","year":"2002","journal-title":"Vox Sang."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"93","DOI":"10.1016\/j.transci.2010.12.012","article-title":"Mass-scale red cell genotyping of blood donors","volume":"44","author":"Denomme","year":"2011","journal-title":"Transfus. Apher. Sci."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"198","DOI":"10.1111\/j.1423-0410.2009.01209.x","article-title":"Blood group genotyping: From patient to high-throughput donor screening","volume":"97","author":"Veldhuisen","year":"2009","journal-title":"Vox Sang."},{"key":"ref_33","doi-asserted-by":"crossref","unstructured":"Reid, M.E., Lomas-Francis, C., and Olsson, M.L. (2012). DO\u2014Dombrock blood group system. The Blood Group Antigen FactsBook, Academic Press. [3rd ed.].","DOI":"10.1016\/B978-0-12-415849-8.00016-8"}],"container-title":["Genes"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2073-4425\/13\/6\/1079\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T23:33:35Z","timestamp":1760139215000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2073-4425\/13\/6\/1079"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,6,17]]},"references-count":33,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2022,6]]}},"alternative-id":["genes13061079"],"URL":"https:\/\/doi.org\/10.3390\/genes13061079","relation":{},"ISSN":["2073-4425"],"issn-type":[{"value":"2073-4425","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,6,17]]}}}