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The identification method of cancer-specific biomarkers has important applications for the diagnosis and treatment of cancer types. In this study, we analyzed the pan-cancer DNA methylation data from TCGA and GEO, and proposed a computational method to quantify the degree of specificity based on the level of DNA methylation of G protein-coupled receptor-related genes (GPCRs-related genes) and to identify specific GPCRs DNA methylation biomarkers (GRSDMs) in pan-cancer. Then, a ridge regression-based method was used to discover potential drugs through predicting the drug sensitivities of cancer samples. Finally, we predicted and verified 8 GRSDMs in adrenocortical carcinoma (ACC), rectum adenocarcinoma (READ), uveal Melanoma (UVM), thyroid carcinoma (THCA), and predicted 4 GRSDMs (F2RL3, DGKB, GRK5, PIK3R6) which were sensitive to 12 potential drugs. Our research provided a novel approach for the personalized diagnosis of cancer and informed individualized treatment decisions.<\/jats:p>","DOI":"10.3390\/genes13071213","type":"journal-article","created":{"date-parts":[[2022,7,7]],"date-time":"2022-07-07T07:51:56Z","timestamp":1657180316000},"page":"1213","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["DNA Methylation-Specific Analysis of G Protein-Coupled Receptor-Related Genes in Pan-Cancer"],"prefix":"10.3390","volume":"13","author":[{"given":"Mengyan","family":"Zhang","sequence":"first","affiliation":[{"name":"Computational Biology Research Center, School of Life Science and Technology, Harbin Institute of Technology, Harbin 150001, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiyun","family":"Zhao","sequence":"additional","affiliation":[{"name":"Computational Biology Research Center, School of Life Science and Technology, Harbin Institute of Technology, Harbin 150001, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huili","family":"Dong","sequence":"additional","affiliation":[{"name":"Computational Biology Research Center, School of Life Science and Technology, Harbin Institute of Technology, Harbin 150001, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenhui","family":"Xue","sequence":"additional","affiliation":[{"name":"Computational Biology Research Center, School of Life Science and Technology, Harbin Institute of Technology, Harbin 150001, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jie","family":"Xing","sequence":"additional","affiliation":[{"name":"Computational Biology Research Center, School of Life Science and Technology, Harbin Institute of Technology, Harbin 150001, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ting","family":"Liu","sequence":"additional","affiliation":[{"name":"College of pathology, Qiqihar Medical University, Qiqihar 161042, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiuwen","family":"Yu","sequence":"additional","affiliation":[{"name":"College of pathology, Qiqihar Medical University, Qiqihar 161042, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yue","family":"Gu","sequence":"additional","affiliation":[{"name":"Computational Biology Research Center, School of Life Science and Technology, Harbin Institute of Technology, Harbin 150001, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Baoqing","family":"Sun","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Respiratory Disease, Guangzhou Medical University, Guangzhou 510089, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haibo","family":"Lu","sequence":"additional","affiliation":[{"name":"Department of Gastrointestinal Medical Oncology, Harbin Medical University Cancer Hospital, Harbin 150000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan","family":"Zhang","sequence":"additional","affiliation":[{"name":"Computational Biology Research Center, School of Life Science and Technology, Harbin Institute of Technology, Harbin 150001, China"},{"name":"College of pathology, Qiqihar Medical University, Qiqihar 161042, China"},{"name":"State Key Laboratory of Respiratory Disease, Guangzhou Medical University, Guangzhou 510089, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,7,7]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"2963","DOI":"10.1039\/C4CS00370E","article-title":"Cancer biomarker detection: Recent achievements and challenges","volume":"44","author":"Wu","year":"2015","journal-title":"Chem. 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