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Ferroptosis is a newly discovered form of regulated cell death involved in multiple autoimmune diseases. Currently, there are no reports on the connection between ferroptosis and AS. Methods: AS samples from the Gene Expression Omnibus were divided into two subgroups using consensus clustering of ferroptosis-related genes (FRGs). Weighted gene co-expression network analysis (WGCNA) of the intergroup differentially expressed genes (DEGs) and protein\u2013protein interaction (PPI) analysis of the key module were used to screen out hub genes. A multifactor regulatory network was then constructed based on hub genes. Results: The 52 AS patients in dataset GSE73754 were divided into cluster 1 (n = 24) and cluster 2 (n = 28). DEGs were mainly enriched in pathways related to mitochondria, ubiquitin, and neurodegeneration. Candidate hub genes, screened by PPI and WGCNA, were intersected. Subsequently, 12 overlapping genes were identified as definitive hub genes. A multifactor interaction network with 45 nodes and 150 edges was generated, comprising the 12 hub genes and 32 non-coding RNAs. Conclusions: AS can be divided into two subtypes according to FRG expression. Ferroptosis might play a regulatory role in AS. Tailoring treatment according to the ferroptosis status of AS patients can be a promising direction.<\/jats:p>","DOI":"10.3390\/genes13081373","type":"journal-article","created":{"date-parts":[[2022,7,31]],"date-time":"2022-07-31T23:37:29Z","timestamp":1659310649000},"page":"1373","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["New Insights into the Regulatory Role of Ferroptosis in Ankylosing Spondylitis via Consensus Clustering of Ferroptosis-Related Genes and Weighted Gene Co-Expression Network Analysis"],"prefix":"10.3390","volume":"13","author":[{"given":"Tianhua","family":"Rong","sequence":"first","affiliation":[{"name":"Department of Orthopaedic Surgery, Spine Center, Beijing Tiantan Hospital, Capital Medical University, Beijing 100070, China"},{"name":"National Clinical Research Center for Orthopedics, Sports Medicine & Rehabilitation, Beijing 100070, China"},{"name":"Basic and Translational Medicine Center, China National Clinical Research Center for Neurological Diseases, Beijing 100070, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ningyi","family":"Jia","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, Beijing Tiantan Hospital, Capital Medical University, Beijing 100070, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bingxuan","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Orthopaedic Surgery, Spine Center, Beijing Tiantan Hospital, Capital Medical University, Beijing 100070, China"},{"name":"National Clinical Research Center for Orthopedics, Sports Medicine & Rehabilitation, Beijing 100070, China"},{"name":"Basic and Translational Medicine Center, China National Clinical Research Center for Neurological Diseases, Beijing 100070, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dacheng","family":"Sang","sequence":"additional","affiliation":[{"name":"Department of Orthopaedic Surgery, Spine Center, Beijing Tiantan Hospital, Capital Medical University, Beijing 100070, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2156-0870","authenticated-orcid":false,"given":"Baoge","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Orthopaedic Surgery, Spine Center, Beijing Tiantan Hospital, Capital Medical University, Beijing 100070, China"},{"name":"National Clinical Research Center for Orthopedics, Sports Medicine & Rehabilitation, Beijing 100070, China"},{"name":"Basic and Translational Medicine Center, China National Clinical Research Center for Neurological Diseases, Beijing 100070, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2022,7,31]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1016\/S0140-6736(16)31591-4","article-title":"Axial spondyloarthritis","volume":"390","author":"Sieper","year":"2017","journal-title":"Lancet"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"ii9","DOI":"10.1136\/ard.2004.033654","article-title":"Seronegative spondyloarthropathies: To lump or split?","volume":"64","author":"Nash","year":"2005","journal-title":"Ann. 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