{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T10:10:29Z","timestamp":1777630229529,"version":"3.51.4"},"reference-count":72,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2022,9,16]],"date-time":"2022-09-16T00:00:00Z","timestamp":1663286400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"statutory funding for young researchers-doctoral students of Poznan University of Medical Sciences for 2021"},{"name":"USDA-NIFA regional project 1184"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Genes"],"abstract":"<jats:p>The interest in stem cell research continuously increased over the last decades, becoming one of the most important trends in the 21st century medicine. Stem cell-based therapies have a potential to become a solution for a range of currently untreatable diseases, such as spinal cord injuries, type I diabetes, Parkinson\u2019s disease, heart disease, stroke, and osteoarthritis. Hence, this study, based on canine material, aims to investigate the molecular basis of adipose-derived stem cell (ASC) differentiation into chondrocytes, to serve as a transcriptomic reference for further research aiming to introduce ASC into treatment of bone and cartilage related diseases, such as osteoarthritis in veterinary medicine. Adipose tissue samples were harvested from a canine specimen subjected to a routine ovariohysterecromy procedure at an associated veterinary clinic. The material was treated for ASC isolation and chondrogenic differentiation. RNA samples were isolated at day 1 of culture, day 30 of culture in unsupplemented culture media, and day 30 of culture in chondrogenic differentiation media. The resulting RNA was analyzed using RNAseq assays, with the results validated by RT-qPCR. Between differentiated chondrocytes, early and late cultures, most up- and down-regulated genes in each comparison were selected for further analysis., there are several genes (e.g., MMP12, MPEG1, CHI3L1, and CD36) that could be identified as new markers of chondrogenesis and the influence of long-term culture conditions on ASCs. The results of the study prove the usefulness of the in vitro culture model, providing further molecular insight into the processes associated with ASC culture and differentiation. Furthermore, the knowledge obtained could be used as a molecular reference for future in vivo and clinical studies.<\/jats:p>","DOI":"10.3390\/genes13091664","type":"journal-article","created":{"date-parts":[[2022,9,18]],"date-time":"2022-09-18T23:39:22Z","timestamp":1663544362000},"page":"1664","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Expression Profile of New Gene Markers Involved in Differentiation of Canine Adipose-Derived Stem Cells into Chondrocytes"],"prefix":"10.3390","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9476-0235","authenticated-orcid":false,"given":"Maurycy","family":"Jankowski","sequence":"first","affiliation":[{"name":"Department of Anatomy, Poznan University of Medical Sciences, 60-701 Poznan, Poland"},{"name":"Doctoral School, Poznan University of Medical Sciences, 60-701 Poznan, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6282-3251","authenticated-orcid":false,"given":"Mariusz","family":"Kaczmarek","sequence":"additional","affiliation":[{"name":"Department of Cancer Immunology, Chair of Medical Biotechnology, Poznan University of Medical Sciences, 61-866 Poznan, Poland"},{"name":"Gene Therapy Laboratory, Department of Cancer Diagnostics and Immunology, Greater Poland Cancer Centre, 61-866 Poznan, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Grzegorz","family":"W\u0105siatycz","sequence":"additional","affiliation":[{"name":"Department of Veterinary Surgery, Institute of Veterinary Medicine, Nicolaus Copernicus University in Torun, 87-100 Torun, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aneta","family":"Konwerska","sequence":"additional","affiliation":[{"name":"Department of Histology and Embryology, Poznan University of Medical Sciences, 60-701 Poznan, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Claudia","family":"Dompe","sequence":"additional","affiliation":[{"name":"Center for Advanced Technology, Department of Molecular and Cellular Biology, Institute of Molecular Biology and Biotechnology, Faculty of Biology, Adam Mickiewicz University, 61-614 Poznan, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dorota","family":"Bukowska","sequence":"additional","affiliation":[{"name":"Department of Diagnostics and Clinical Sciences, Institute of Veterinary Medicine, Nicolaus Copernicus University in Torun, 87-100 Torun, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pawe\u0142","family":"Antosik","sequence":"additional","affiliation":[{"name":"Department of Veterinary Surgery, Institute of Veterinary Medicine, Nicolaus Copernicus University in Torun, 87-100 Torun, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1575-3123","authenticated-orcid":false,"given":"Paul","family":"Mozdziak","sequence":"additional","affiliation":[{"name":"Prestage Department of Poultry Science, North Carolina State University, Raleigh, NC 27695, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bartosz","family":"Kempisty","sequence":"additional","affiliation":[{"name":"Department of Anatomy, Poznan University of Medical Sciences, 60-701 Poznan, Poland"},{"name":"Department of Veterinary Surgery, Institute of Veterinary Medicine, Nicolaus Copernicus University in Torun, 87-100 Torun, Poland"},{"name":"Department of Histology and Embryology, Poznan University of Medical Sciences, 60-701 Poznan, Poland"},{"name":"Prestage Department of Poultry Science, North Carolina State University, Raleigh, NC 27695, USA"},{"name":"Department of Obstetrics and Gynecology, University Hospital and Masaryk University, 601 77 Brno, Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2022,9,16]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"134","DOI":"10.1302\/2046-3758.102.BJR-2020-0517.R1","article-title":"Current Status of Regenerative Medicine in Osteoarthritis","volume":"10","author":"Im","year":"2021","journal-title":"Bone Jt. Res."},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"De la Torre, P., and Flores, A.I. (2020). Current Status and Future Prospects of Perinatal Stem Cells. Genes, 12.","DOI":"10.3390\/genes12010006"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"134","DOI":"10.2478\/acb-2019-0018","article-title":"Ovarian Follicular Cells\u2014Living in the Shadow of Stemness Cellular Competence","volume":"7","author":"Moncrieff","year":"2019","journal-title":"Med. J. Cell Biol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"101","DOI":"10.2478\/acb-2018-0016","article-title":"The Use of Mesenchymal Stem Cells in Veterinary Medicine","volume":"6","author":"Kulus","year":"2018","journal-title":"Med. J. Cell Biol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"183","DOI":"10.2478\/acb-2019-0025","article-title":"Evidence for Existence of Molecular Stemness Markers in Porcine Ovarian Follicular Granulosa Cells","volume":"7","author":"Sibiak","year":"2019","journal-title":"Med. J. Cell Biol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"27","DOI":"10.2478\/acb-2018-0005","article-title":"The Differentiation and Transdifferentiation of Epithelial Cells In Vitro\u2014Is It a New Strategy in Regenerative Biomedicine?","volume":"6","author":"Jankowski","year":"2018","journal-title":"Med. J. Cell Biol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"18","DOI":"10.32607\/20758251-2010-2-2-18-27","article-title":"Induced Pluripotent Stem Cells: Problems and Advantages When Applying Them in Regenerative Medicine","volume":"2","author":"Medvedev","year":"2010","journal-title":"Acta Nat."},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Jarrige, M., Frank, E., Herardot, E., Martineau, S., Darle, A., Benabides, M., Domingues, S., Chose, O., Habeler, W., and Lorant, J. (2021). The Future of Regenerative Medicine: Cell Therapy Using Pluripotent Stem Cells and Acellular Therapies Based on Extracellular Vesicles. Cells, 10.","DOI":"10.3390\/cells10020240"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"105","DOI":"10.2478\/acb-2019-0014","article-title":"Current Clinical Applications of Adipose-Derived Stem Cells in Humans and Animals","volume":"7","author":"Dompe","year":"2019","journal-title":"Med. J. Cell Biol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"14","DOI":"10.3389\/fcell.2021.621214","article-title":"Hematopoietic Stem Cell Niche During Homeostasis, Malignancy, and Bone Marrow Transplantation","volume":"9","author":"Man","year":"2021","journal-title":"Front. Cell Dev. Biol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.hoc.2010.11.001","article-title":"A History of Bone Marrow Transplantation","volume":"25","author":"Gatti","year":"2011","journal-title":"Hematol. Oncol. Clin. N. Am."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1002\/ar.a.10134","article-title":"Adult Stem Cells","volume":"276","author":"Young","year":"2004","journal-title":"Anat. Rec.\u2014Part A Discov. Mol. Cell. Evol. Biol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"114","DOI":"10.2478\/acb-2018-0018","article-title":"Stemness Specificity of Epithelial Cells\u2014Application of Cell and Tissue Technology in Regenerative Medicine","volume":"6","author":"Rojewska","year":"2018","journal-title":"Med. J. Cell Biol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"83","DOI":"10.2478\/acb-2020-0010","article-title":"Mesenchymal Stem Cells\u2014A Historical Overview","volume":"8","author":"Stefanska","year":"2020","journal-title":"Med. J. Cell Biol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"78","DOI":"10.2478\/acb-2020-0009","article-title":"Human Umbilical Cord Stem Cells-the Discovery, History and Possible Application","volume":"8","author":"Stefanska","year":"2020","journal-title":"Med. J. Cell Biol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"124","DOI":"10.2478\/acb-2020-0016","article-title":"Current Stem Cells Technologies Used in Medicine","volume":"8","author":"Czarnecka","year":"2020","journal-title":"Med. J. Cell Biol."},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Jankowski, M., Dompe, C., Sibiak, R., W\u0105siatycz, G., Mozdziak, P., Ja\u015bkowski, J.M., Antosik, P., Kempisty, B., and Dyszkiewicz-Konwi\u0144ska, M. (2020). In Vitro Cultures of Adipose-Derived Stem Cells: An Overview of Methods, Molecular Analyses, and Clinical Applications. Cells, 9.","DOI":"10.3390\/cells9081783"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"685","DOI":"10.3233\/JAD-2008-15413","article-title":"The Canine (Dog) Model of Human Aging and Disease: Dietary, Environmental and Immunotherapy Approaches","volume":"15","author":"Cotman","year":"2008","journal-title":"J. Alzheimer\u2019s Dis."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"e1241362","DOI":"10.1080\/21675511.2016.1241362","article-title":"Canine Models of Human Rare Disorders","volume":"4","author":"Lohi","year":"2016","journal-title":"Rare Dis."},{"key":"ref_20","first-page":"484","article-title":"Cell Surface Markers on Adipose-Derived Stem Cells: A Systematic Review","volume":"12","author":"Khan","year":"2017","journal-title":"Curr. Stem Cell Res. Ther."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"315","DOI":"10.1080\/14653240600855905","article-title":"Minimal Criteria for Defining Multipotent Mesenchymal Stromal Cells. The International Society for Cellular Therapy Position Statement","volume":"8","author":"Dominici","year":"2006","journal-title":"Cytotherapy"},{"key":"ref_22","doi-asserted-by":"crossref","unstructured":"Chermu\u0142a, B., Brazert, M., Jeseta, M., O\u017cegowska, K., Sujka-Kordowska, P., Konwerska, A., Bryja, A., Kranc, W., Jankowski, M., and Nawrocki, M.J. (2019). The Unique Mechanisms of Cellular Proliferation, Migration and Apoptosis Are Regulated through Oocyte Maturational Development\u2014A Complete Transcriptomic and Histochemical Study. Int. J. Mol. Sci., 20.","DOI":"10.3390\/ijms20010084"},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"Jiang, H., Lei, R., Ding, S.W., and Zhu, S. (2014). Skewer: A Fast and Accurate Adapter Trimmer for next-Generation Sequencing Paired-End Reads. BMC Bioinform., 15.","DOI":"10.1186\/1471-2105-15-182"},{"key":"ref_24","first-page":"D682","article-title":"Ensembl 2020","volume":"48","author":"Yates","year":"2020","journal-title":"Nucleic Acids Res."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"1937","DOI":"10.1093\/bioinformatics\/bty036","article-title":"Primer3-Masker: Integrating Masking of Template Sequence with Primer Design Software","volume":"34","author":"Lepamets","year":"2018","journal-title":"Bioinformatics"},{"key":"ref_26","first-page":"71","article-title":"An Improvement of the 2\u02c6(-Delta Delta CT) Method for Quantitative Real-Time Polymerase Chain Reaction Data Analysis","volume":"3","author":"Rao","year":"2013","journal-title":"Biostat. Bioinform. Biomath."},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Ge, S.X., Son, E.W., and Yao, R. (2018). IDEP: An Integrated Web Application for Differential Expression and Pathway Analysis of RNA-Seq Data. BMC Bioinform., 19.","DOI":"10.1186\/s12859-018-2486-6"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1093\/bioinformatics\/btp616","article-title":"EdgeR: A Bioconductor Package for Differential Expression Analysis of Digital Gene Expression Data","volume":"26","author":"Robinson","year":"2010","journal-title":"Bioinformatics"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"D607","DOI":"10.1093\/nar\/gky1131","article-title":"STRING V11: Protein-Protein Association Networks with Increased Coverage, Supporting Functional Discovery in Genome-Wide Experimental Datasets","volume":"47","author":"Szklarczyk","year":"2019","journal-title":"Nucleic Acids Res."},{"key":"ref_30","doi-asserted-by":"crossref","unstructured":"Jankowski, M., Kaczmarek, M., W\u0105siatycz, G., Dompe, C., Mozdziak, P., Ja\u015bkowski, J.M., Piotrowska-Kempisty, H., and Kempisty, B. (2021). Expression Profile of New Marker Genes Involved in Differentiation of Canine Adipose-Derived Stem Cells into Osteoblasts. Int. J. Mol. Sci., 22.","DOI":"10.3390\/ijms22136663"},{"key":"ref_31","doi-asserted-by":"crossref","unstructured":"Dyszkiewicz-Konwi\u0144ska, M., Nawrocki, M., Huang, Y., Bryja, A., Celichowski, P., Jankowski, M., B\u0142ochowiak, K., Mehr, K., Bruska, M., and Nowicki, M. (2018). New Gene Markers for Metabolic Processes and Homeostasis in Porcine Buccal Pouch Mucosa during Cells Long Term-Cultivation\u2014A Primary Culture Approach. Int. J. Mol. Sci., 19.","DOI":"10.3390\/ijms19041027"},{"key":"ref_32","doi-asserted-by":"crossref","unstructured":"Kranc, W., Br\u0105zert, M., O\u017cegowska, K., Nawrocki, M.M.J., Budna, J., Celichowski, P., Dyszkiewicz-Konwi\u0144ska, M., Jankowski, M., Jeseta, M., and Pawelczyk, L. (2017). Expression Profile of Genes Regulating Steroid Biosynthesis and Metabolism in Human Ovarian Granulosa Cells\u2014A Primary Culture Approach. Int. J. Mol. Sci., 18.","DOI":"10.3390\/ijms18122673"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"721","DOI":"10.1136\/jmedgenet-2011-100242","article-title":"Studying the Epigenome Using next Generation Sequencing","volume":"48","author":"Ku","year":"2011","journal-title":"J. Med. Genet."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"5316","DOI":"10.1091\/mbc.e05-01-0084","article-title":"Microarray Analyses of Gene Expression during Chondrocyte Differentiation Identifies Novel Regulators of Hypertrophy","volume":"16","author":"James","year":"2005","journal-title":"Mol. Biol. Cell"},{"key":"ref_35","first-page":"213","article-title":"Expression of CHI3L1 and CHIT1 in Osteoarthritic Rat Cartilage Model. A Morphological Study","volume":"58","author":"Szychlinska","year":"2014","journal-title":"Eur. J. Histochem."},{"key":"ref_36","doi-asserted-by":"crossref","unstructured":"Szychlinska, M.A., Trovato, F.M., Di Rosa, M., Malaguarnera, L., Puzzo, L., Leonardi, R., Castrogiovanni, P., and Musumeci, G. (2016). Co-Expression and Co-Localization of Cartilage Glycoproteins CHI3L1 and Lubricin in Osteoarthritic Cartilage: Morphological, Immunohistochemical and Gene Expression Profiles. Int. J. Mol. Sci., 17.","DOI":"10.3390\/ijms17030359"},{"key":"ref_37","doi-asserted-by":"crossref","unstructured":"LaPointe, V.L.S., Verpoorte, A., and Stevens, M.M. (2013). The Changing Integrin Expression and a Role for Integrin \u03928 in the Chondrogenic Differentiation of Mesenchymal Stem Cells. PLoS ONE, 8.","DOI":"10.1371\/journal.pone.0082035"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"5024","DOI":"10.4049\/jimmunol.0803603","article-title":"The Pattern Recognition Receptor CD36 Is a Chondrocyte Hypertrophy Marker Associated with Suppression of Catabolic Responses and Promotion of Repair Responses to Inflammatory Stimuli","volume":"182","author":"Bendele","year":"2009","journal-title":"J. Immunol."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"487","DOI":"10.1016\/S8756-3282(01)00595-6","article-title":"Human Macrophage Metalloelastase (MMP-12) Expression Is Induced in Chondrocytes during Fetal Development and Malignant Transformation","volume":"29","author":"Herva","year":"2001","journal-title":"Bone"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"5120","DOI":"10.1074\/jbc.M506667200","article-title":"Stanniocalcin 1 Acts as a Paracrine Regulator of Growth Plate Chondrogenesis*","volume":"281","author":"Wu","year":"2006","journal-title":"J. Biol. Chem."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"403","DOI":"10.1007\/s00418-021-02067-9","article-title":"Caspase-9 Inhibition Decreases Expression of Mmp9 during Chondrogenesis","volume":"157","author":"Ramesova","year":"2022","journal-title":"Histochem. Cell Biol."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"163","DOI":"10.1016\/j.micpath.2016.09.020","article-title":"A Novel Missense Mutation in the NADPH Binding Domain of CYBB Abolishes the NADPH Oxidase Activity in a Male Patient with Increased Susceptibility to Infections","volume":"100","author":"Khan","year":"2016","journal-title":"Microb. Pathog."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"820","DOI":"10.1007\/s12035-014-8811-9","article-title":"TYROBP in Alzheimer\u2019s Disease","volume":"51","author":"Ma","year":"2015","journal-title":"Mol. Neurobiol."},{"key":"ref_44","doi-asserted-by":"crossref","unstructured":"Liang, T., Chen, J., Xu, G.Y., Zhang, Z., Xue, J., Zeng, H., Jiang, J., Chen, T., Qin, Z., and Li, H. (2022). STAT3 and SPI1, May Lead to the Immune System Dysregulation and Heterotopic Ossification in Ankylosing Spondylitis. BMC Immunol., 23.","DOI":"10.1186\/s12865-022-00476-6"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"144","DOI":"10.1007\/s00774-011-0303-7","article-title":"3\u03b2-Hydroxysterol-Delta24 Reductase Plays an Important Role in Long Bone Growth by Protecting Chondrocytes from Reactive Oxygen Species","volume":"30","author":"Mirza","year":"2012","journal-title":"J. Bone Miner. Metab."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"184","DOI":"10.1186\/s13287-016-0445-6","article-title":"Centrifugal Gravity-Induced BMP4 Induces Chondrogenic Differentiation of Adipose-Derived Stem Cells via SOX9 Upregulation","volume":"7","author":"Jang","year":"2016","journal-title":"Stem Cell Res. Ther."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"249","DOI":"10.1111\/j.1365-2184.2010.00680.x","article-title":"Soluble Factors from ASCs Effectively Direct Control of Chondrogenic Fate","volume":"43","author":"Kim","year":"2010","journal-title":"Cell Prolif."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"3428","DOI":"10.1242\/jcs.083659","article-title":"BMP2, but Not BMP4, Is Crucial for Chondrocyte Proliferation and Maturation during Endochondral Bone Development","volume":"124","author":"Shu","year":"2011","journal-title":"J. Cell Sci."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"10","DOI":"10.1186\/s13287-018-1103-y","article-title":"Cell Number in Mesenchymal Stem Cell Aggregates Dictates Cell Stiffness and Chondrogenesis","volume":"10","author":"Sarem","year":"2019","journal-title":"Stem Cell Res. Ther."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"4331","DOI":"10.1158\/0008-5472.CAN-08-0943","article-title":"Carcinoma-Associated Fibroblast\u2013Like Differentiation of Human Mesenchymal Stem Cells","volume":"68","author":"Mishra","year":"2008","journal-title":"Cancer Res."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"8935","DOI":"10.1038\/s41598-017-09335-6","article-title":"Integrative Epigenomics, Transcriptomics and Proteomics of Patient Chondrocytes Reveal Genes and Pathways Involved in Osteoarthritis","volume":"7","author":"Steinberg","year":"2017","journal-title":"Sci. Rep."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"273","DOI":"10.1016\/j.gene.2004.11.008","article-title":"Biochemical Characterization of the Mammalian Cux2 Protein","volume":"344","author":"Gingras","year":"2005","journal-title":"Gene"},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"529","DOI":"10.1093\/hmg\/ddx422","article-title":"DNAAF1 Links Heart Laterality with the AAA+ ATPase RUVBL1 and Ciliary Intraflagellar Transport","volume":"27","author":"Hartill","year":"2018","journal-title":"Hum. Mol. Genet."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"3307","DOI":"10.1534\/g3.116.033696","article-title":"Mutations in the Motile Cilia Gene DNAAF1 Are Associated with Neural Tube Defects in Humans","volume":"6","author":"Miao","year":"2016","journal-title":"G3 Genes Genomes Genet."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"305","DOI":"10.1007\/s00406-011-0270-y","article-title":"Influence of GRIA1, GRIA2 and GRIA4 Polymorphisms on Diagnosis and Response to Treatment in Patients with Major Depressive Disorder","volume":"262","author":"Chiesa","year":"2012","journal-title":"Eur. Arch. Psychiatry Clin. Neurosci."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"24","DOI":"10.1002\/ajmg.b.30780","article-title":"Polymorphisms in the GRIA1 Gene Region in Psychotic Bipolar Disorder","volume":"150","author":"Kerner","year":"2009","journal-title":"Am. J. Med. Genet. Part B Neuropsychiatr. Genet."},{"key":"ref_57","first-page":"1850","article-title":"Analysis of Bladder Cancer Tumor CpG Methylation and Gene Expression within The Cancer Genome Atlas Identifies GRIA1 as a Prognostic Biomarker for Basal-like Bladder Cancer","volume":"7","author":"Tilley","year":"2017","journal-title":"Am. J. Cancer Res."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"1414","DOI":"10.1002\/glia.21186","article-title":"Extensive Aspartoacylase Expression in the Rat Central Nervous System","volume":"59","author":"Moffett","year":"2011","journal-title":"Glia"},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"e1194","DOI":"10.1002\/mgg3.1194","article-title":"The Relaxin Family Peptide Receptor 1 (RXFP1): An Emerging Player in Human Health and Disease","volume":"8","author":"Chen","year":"2020","journal-title":"Mol. Genet. Genom. Med."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"1084","DOI":"10.1016\/j.bone.2008.07.252","article-title":"Gene Expression Analysis in Osteoblastic Differentiation from Peripheral Blood Mesenchymal Stem Cells","volume":"43","author":"Valenti","year":"2008","journal-title":"Bone"},{"key":"ref_61","doi-asserted-by":"crossref","unstructured":"Jiao, K., Zhang, J., Zhang, M., Wei, Y., Wu, Y., Qiu, Z.Y., He, J., Cao, Y., Hu, J., and Zhu, H. (2013). The Identification of CD163 Expressing Phagocytic Chondrocytes in Joint Cartilage and Its Novel Scavenger Role in Cartilage Degradation. PLoS ONE, 8.","DOI":"10.1371\/journal.pone.0053312"},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"351","DOI":"10.15252\/embj.201796781","article-title":"LRRC25 Inhibits Type I IFN Signaling by Targeting ISG15-Associated RIG-I for Autophagic Degradation","volume":"37","author":"Du","year":"2018","journal-title":"EMBO J."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"575","DOI":"10.1016\/j.stemcr.2013.10.012","article-title":"Human Developmental Chondrogenesis as a Basis for Engineering Chondrocytes from Pluripotent Stem Cells","volume":"1","author":"Wu","year":"2013","journal-title":"Stem Cell Rep."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1016\/S0303-7207(02)00013-8","article-title":"Prolactin Is a Component of the Human Synovial Liquid and Modulates the Growth and Chondrogenic Differentiation of Bone Marrow-Derived Mesenchymal Stem Cells","volume":"190","author":"Ogueta","year":"2002","journal-title":"Mol. Cell. Endocrinol."},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"108","DOI":"10.1016\/j.bbrc.2012.02.123","article-title":"Chondroregulatory Action of Prolactin on Proliferation and Differentiation of Mouse Chondrogenic ATDC5 Cells in 3-Dimensional Micromass Cultures","volume":"420","author":"Seriwatanachai","year":"2012","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"1101","DOI":"10.1097\/BRS.0b013e3181c0c727","article-title":"Differential Gene Expression Profiling of Metalloproteinases and Their Inhibitors: A Comparison between Bovine Intervertebral Disc Nucleus Pulposus Cells and Articular Chondrocytes","volume":"35","author":"Cui","year":"2010","journal-title":"Spine"},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"2393","DOI":"10.1002\/jcp.25371","article-title":"The Impact of Epigenetics on Mesenchymal Stem Cell Biology","volume":"231","author":"Ozkul","year":"2016","journal-title":"J. Cell. Physiol."},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"1901669","DOI":"10.1002\/adhm.201901669","article-title":"Extracellular Matrix Production by Mesenchymal Stromal Cells in Hydrogels Facilitates Cell Spreading and Is Inhibited by FGF-2","volume":"9","author":"Horton","year":"2020","journal-title":"Adv. Healthc. Mater."},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"1611","DOI":"10.4161\/cc.20023","article-title":"Expression of the 49 Human ATP Binding Cassette (ABC) Genes in Pluripotent Embryonic Stem Cells and in Early- and Late-Stage Multipotent Mesenchymal Stem Cells: Possible Role of ABC Plasma Membrane Transporters in Maintaining Human Stem Cell Pluripotency","volume":"11","author":"Barbet","year":"2012","journal-title":"Cell Cycle"},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"1311","DOI":"10.1089\/hum.2010.246","article-title":"Current Status and Prospects of Gene Therapy for the Inner Ear","volume":"22","author":"Sun","year":"2011","journal-title":"Hum. Gene Ther."},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"771","DOI":"10.1038\/s41586-022-04590-8","article-title":"Tethered Peptide Activation Mechanism of the Adhesion GPCRs ADGRG2 and ADGRG4","volume":"604","author":"Xiao","year":"2022","journal-title":"Nature"},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"3176","DOI":"10.1093\/brain\/awz233","article-title":"Genome-Wide Association Study of Cerebral Small Vessel Disease Reveals Established and Novel Loci","volume":"142","author":"Chung","year":"2019","journal-title":"Brain"}],"container-title":["Genes"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2073-4425\/13\/9\/1664\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T00:33:18Z","timestamp":1760142798000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2073-4425\/13\/9\/1664"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,9,16]]},"references-count":72,"journal-issue":{"issue":"9","published-online":{"date-parts":[[2022,9]]}},"alternative-id":["genes13091664"],"URL":"https:\/\/doi.org\/10.3390\/genes13091664","relation":{},"ISSN":["2073-4425"],"issn-type":[{"value":"2073-4425","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,9,16]]}}}