{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T04:43:55Z","timestamp":1787028235308,"version":"build-2736575974"},"reference-count":69,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2022,10,2]],"date-time":"2022-10-02T00:00:00Z","timestamp":1664668800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Genes"],"abstract":"<jats:p>Preeclampsia is a leading cause of perinatal maternal-foetal mortality and morbidity. This study aims to identify the key microRNAs (miRNA) in preeclampsia and uncover their potential functions. We downloaded the miRNA expression profile of GSE119799 for plasma and GSE177049 for the placenta. Each dataset consisted of five patients (PE) and five controls (N). From a technical point of view, we analysed the counts per million (CPM) for both datasets, highlighting 358 miRNAs in common, 78 unique for plasma and 298 unique for placenta. At the same time, we performed an expression differential analysis (|logFC| \u2265 1|and FDR \u2264 0.05) to evaluate the biological impact of the miRNAs. This approach allowed us to highlight 321 miRNAs in common between plasma and placenta, within which four were upregulated in plasma. Furthermore, the same analysis revealed five miRNAs expressed exclusively in plasma; these were also upregulated. In conclusion, the in-depth bioinformatics analysis conducted during our study will allow us, on the one hand, to verify the targets of each of the nine identified miRNAs; on the other hand, to use them both as new non-invasive biomarkers and as therapeutic targets for the development of personalised treatments.<\/jats:p>","DOI":"10.3390\/genes13101781","type":"journal-article","created":{"date-parts":[[2022,10,8]],"date-time":"2022-10-08T20:43:21Z","timestamp":1665261801000},"page":"1781","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Integrated Bioinformatics Analysis Reveals Novel miRNA as Biomarkers Associated with Preeclampsia"],"prefix":"10.3390","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4161-512X","authenticated-orcid":false,"given":"Mariarita","family":"Brancaccio","sequence":"first","affiliation":[{"name":"Department of Molecular Medicine and Medical Biotechnology, University of Naples Federico II, 80131 Naples, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Caterina","family":"Giachino","sequence":"additional","affiliation":[{"name":"Department of Biology, University of Naples Federico II, 80126 Naples, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0733-1438","authenticated-orcid":false,"given":"Assunta Maria","family":"Iazzetta","sequence":"additional","affiliation":[{"name":"Department of Biology, University of Naples Federico II, 80126 Naples, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antonio","family":"Cordone","sequence":"additional","affiliation":[{"name":"Department of Biology, University of Naples Federico II, 80126 Naples, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elena","family":"De Marino","sequence":"additional","affiliation":[{"name":"Department of Biology, University of Naples Federico II, 80126 Naples, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ornella","family":"Affinito","sequence":"additional","affiliation":[{"name":"Department of Molecular Medicine and Medical Biotechnology, University of Naples Federico II, 80131 Naples, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2458-4226","authenticated-orcid":false,"given":"Maria","family":"Vivo","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Biology, University of Salerno, 84084 Fisciano, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6508-8889","authenticated-orcid":false,"given":"Viola","family":"Calabr\u00f2","sequence":"additional","affiliation":[{"name":"Department of Biology, University of Naples Federico II, 80126 Naples, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alessandra","family":"Pollice","sequence":"additional","affiliation":[{"name":"Department of Biology, University of Naples Federico II, 80126 Naples, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5940-5278","authenticated-orcid":false,"given":"Tiziana","family":"Angrisano","sequence":"additional","affiliation":[{"name":"Department of Biology, University of Naples Federico II, 80126 Naples, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,10,2]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"335","DOI":"10.1046\/j.1468-0734.2001.00048.x","article-title":"Von Willebrand factors and von Willebrand disease","volume":"5","author":"Budde","year":"2001","journal-title":"Rev. Clin. Exp. Hematol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"657","DOI":"10.1055\/s-2001-18870","article-title":"Fibrin degradation products, fibrin monomer and soluble fibrin in disseminated intravascular coagulation","volume":"27","author":"Horan","year":"2001","journal-title":"Semin. Thromb. Hemost."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"734","DOI":"10.2741\/4240","article-title":"Endothelial dysfunction in the pathogenesis of pre-eclampsia","volume":"19","author":"Breborowicz","year":"2014","journal-title":"Front. Biosci.-Landmark"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"525","DOI":"10.9745\/GHSP-D-15-00350","article-title":"Pre-eclampsia as Underlying Cause for Perinatal Deaths: Time for Action","volume":"3","author":"Hodgins","year":"2015","journal-title":"Glob. Health Sci. Pract."},{"key":"ref_5","first-page":"2317","article-title":"Diagnosis and management of preeclampsia","volume":"70","author":"Wagner","year":"2004","journal-title":"Am. Fam. Physician"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"350","DOI":"10.1038\/nature02871","article-title":"The functions of animal microRNAs","volume":"431","author":"Ambros","year":"2004","journal-title":"Nature"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"281","DOI":"10.1016\/S0092-8674(04)00045-5","article-title":"MicroRNAs: Genomics, biogenesis, mechanism, and function","volume":"116","author":"Bartel","year":"2004","journal-title":"Cell"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"215","DOI":"10.1016\/j.cell.2009.01.002","article-title":"MicroRNAs: Target recognition and regulatory functions","volume":"136","author":"Bartel","year":"2009","journal-title":"Cell"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"1339","DOI":"10.1681\/ASN.2020010031","article-title":"Dysregulation of Principal Cell miRNAs Facilitates Epigenetic Regulation of AQP2 and Results in Nephrogenic Diabetes Insipidus","volume":"32","author":"Petrillo","year":"2021","journal-title":"J. Am. Soc. Nephrol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"28","DOI":"10.2174\/2211536607666180813123303","article-title":"MicroRNAs in Preeclampsia","volume":"8","author":"Skalis","year":"2019","journal-title":"Microrna"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"2317","DOI":"10.21873\/invivo.12044","article-title":"Deep Sequencing Identified Dysregulated Circulating MicroRNAs in Late Onset Preeclampsia","volume":"34","author":"Mavreli","year":"2020","journal-title":"In Vivo"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"22","DOI":"10.3892\/mmr.2016.5268","article-title":"Placental microRNA expression in pregnancies complicated by superimposed pre-eclampsia on chronic hypertension","volume":"14","author":"Vashukova","year":"2016","journal-title":"Mol. Med. Rep."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1093\/bioinformatics\/btp616","article-title":"edgeR: A Bioconductor package for differential expression analysis of digital gene expression data","volume":"26","author":"Robinson","year":"2010","journal-title":"Bioinformatics"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"D127","DOI":"10.1093\/nar\/gkz757","article-title":"miRDB: An online database for prediction of functional microRNA targets","volume":"48","author":"Chen","year":"2020","journal-title":"Nucleic Acids Res."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"18","DOI":"10.1186\/s13059-019-1629-z","article-title":"Prediction of functional microRNA targets by integrative modeling of microRNA binding and target expression data","volume":"22","author":"Liu","year":"2019","journal-title":"Genome Biol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"1203","DOI":"10.1016\/j.cell.2006.07.031","article-title":"A pattern-based method for the identification of MicroRNA binding sites and their corresponding heteroduplexes","volume":"126","author":"Miranda","year":"2006","journal-title":"Cell"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"1741","DOI":"10.1126\/science.aav1741","article-title":"The biochemical basis of microRNA targeting efficacy","volume":"20","author":"McGeary","year":"2019","journal-title":"Science"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"05005","DOI":"10.7554\/eLife.05005","article-title":"Predicting effective microRNA target sites in mammalian mRNAs","volume":"4","author":"Agarwal","year":"2015","journal-title":"eLife"},{"key":"ref_19","first-page":"1139","article-title":"Weak Seed-Pairing Stability and High Target-Site Abundance Decrease the Proficiency of lsy-6 and Other miRNAs","volume":"8","author":"Baek","year":"2011","journal-title":"Nat. Struct. Mol. Biol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"92","DOI":"10.1101\/gr.082701.108","article-title":"Most Mammalian mRNAs Are Conserved Targets of MicroRNAs","volume":"19","author":"Friedman","year":"2009","journal-title":"Genome Res."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"91","DOI":"10.1016\/j.molcel.2007.06.017","article-title":"MicroRNA Targeting Specificity in Mammals: Determinants beyond Seed Pairing","volume":"27","author":"Grimson","year":"2007","journal-title":"Mol. Cell"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1016\/j.cell.2004.12.035","article-title":"Conserved Seed Pairing, Often Flanked by Adenosines, Indicates that Thousands of Human Genes are MicroRNA Targets","volume":"120","author":"Lewis","year":"2005","journal-title":"Cell"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"W244","DOI":"10.1093\/nar\/gkaa467","article-title":"miRNet 2.0\u2014Network-based visual analytics for miRNA functional analysis and systems biology","volume":"48","author":"Chang","year":"2020","journal-title":"Nucl. Acids Res."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"2022","DOI":"10.1210\/en.2017-03024","article-title":"Redox-Sensitive Transcription Factor NRF2 Enhances Trophoblast Differentiation via Induction of miR-1246 and Aromatase","volume":"159","author":"Muralimanoharan","year":"2018","journal-title":"Endocrinology"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"14","DOI":"10.1186\/s12929-021-00712-y","article-title":"Glutaminase isoforms expression switches microRNA levels and oxidative status in glioblastoma cells","volume":"28","author":"Lobo","year":"2021","journal-title":"J. Biomed. Sci."},{"key":"ref_26","first-page":"5223","article-title":"MiRNA-203a-3p inhibits inflammatory response in preeclampsia through regulating IL24","volume":"24","author":"Ma","year":"2020","journal-title":"Eur. Rev. Med. Pharmacol. Sci."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"9140","DOI":"10.1080\/21655979.2021.1993717","article-title":"Pathogenic mechanisms of preeclampsia with severe features implied by the plasma exosomal mirna profile","volume":"12","author":"Chen","year":"2021","journal-title":"Bioengineered"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"249","DOI":"10.1210\/en.2018-00836","article-title":"\u00f9 Circulating Exosomal miRNA Profile During Term and Preterm Birth Pregnancies: A Longitudinal Study","volume":"160","author":"Menon","year":"2019","journal-title":"Endocrinology"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"E836","DOI":"10.1152\/ajpendo.00660.2012","article-title":"Differential miRNA expression profiles between the first and third trimester human placentas","volume":"304","author":"Gu","year":"2013","journal-title":"Am. J. Physiol. Endocrinol. Metab."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"114","DOI":"10.1002\/kjm2.12144","article-title":"Comprehensive analysis of the differential expression profile of microRNAs in missed abortion","volume":"36","author":"Tian","year":"2020","journal-title":"Kaohsiung J. Med. Sci."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"712150","DOI":"10.3389\/fgene.2021.712150","article-title":"Construction of Circulating MicroRNAs-Based Non-invasive Prediction Models of Recurrent Implantation Failure by Network Analysis","volume":"12","author":"Chen","year":"2021","journal-title":"Front. Genet."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"868","DOI":"10.3945\/an.117.015800","article-title":"Role of MicroRNA Regulation in Obesity-Associated Breast Cancer: Nutritional Perspectives","volume":"8","author":"Kasiappan","year":"2017","journal-title":"Adv. Nutr."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"e465","DOI":"10.1038\/emm.2017.311","article-title":"MicroRNA-708-3p as a potential therapeutic target via the ADAM17-GATA\/STAT3 axis in idiopathic pulmonary fibrosis","volume":"50","author":"Liu","year":"2018","journal-title":"Exp. Mol. Med."},{"key":"ref_34","doi-asserted-by":"crossref","unstructured":"Hayakawa, K., Kawasaki, M., Hirai, T., Yoshida, Y., Tsushima, H., Fujishiro, M., Ikeda, K., Morimoto, S., Takamori, K., and Sekigawa, I. (2019). MicroRNA-766-3p Contributes to Anti-Inflammatory Responses through the Indirect Inhibition of NF-\u03baB Signaling. Int. J. Mol. Sci., 20.","DOI":"10.3390\/ijms20040809"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"664","DOI":"10.7150\/ijms.41999","article-title":"Global Analysis of miRNA Signature Differentially Expressed in Insulin-resistant Human Hepatocellular Carcinoma Cell Line","volume":"17","author":"Li","year":"2020","journal-title":"Int. J. Med. Sci."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"1149","DOI":"10.1080\/15384047.2016.1235661","article-title":"MiR-4500 is epigenetically downregulated in colorectal cancer and functions as a novel tumor suppressor by regulating HMGA2","volume":"17","author":"Yu","year":"2016","journal-title":"Cancer Biol. Ther."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"278","DOI":"10.1016\/j.omtn.2020.04.018","article-title":"MicroRNA-4500 Inhibits Migration, Invasion, and Angiogenesis of Breast Cancer Cells via RRM2-Dependent MAPK Signaling Pathway","volume":"21","author":"Li","year":"2020","journal-title":"Mol. Ther. Nucleic Acids"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"415","DOI":"10.1039\/C8TX00031J","article-title":"Upregulated has-miR-4516 as a potential biomarker for early diagnosis of dust-induced pulmonary fibrosis in patients with pneumoconiosis","volume":"7","author":"Huang","year":"2018","journal-title":"Toxicol. Res."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"1630","DOI":"10.1002\/jcp.24608","article-title":"hsa-miR-4516 mediated downregulation of STAT3\/CDK6\/UBE2N plays a role in PUVA induced apoptosis in keratinocytes","volume":"229","author":"Chowdhari","year":"2014","journal-title":"J. Cell Physiol."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"3142","DOI":"10.1016\/j.bbadis.2017.08.014","article-title":"miR-4516, a microRNA downregulated in psoriasis inhibits keratinocyte motility by targeting fibronectin\/integrin \u03b19 signaling","volume":"1863","author":"Chowdhari","year":"2017","journal-title":"Biochim. Biophys. Acta Mol. Basis Dis."},{"key":"ref_41","doi-asserted-by":"crossref","unstructured":"Umair, Z., Baek, M.O., Song, J., An, S., Chon, S.J., and Yoon, M.S. (2022). MicroRNA-4516 in Urinary Exosomes as a Biomarker of Premature Ovarian Insufficiency. Cells, 11.","DOI":"10.3390\/cells11182797"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"32","DOI":"10.1186\/s12967-020-02227-w","article-title":"Clinical application of exosomes and circulating microRNAs in the diagnosis of pregnancy complications and foetal abnormalities","volume":"18","author":"Yang","year":"2020","journal-title":"J. Transl. Med."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"106","DOI":"10.1016\/j.anl.2018.05.005","article-title":"MicroRNA-4497 functions as a tumor suppressor in laryngeal squamous cell carcinoma via negatively modulation the GBX2","volume":"46","author":"Chen","year":"2019","journal-title":"Auris Nasus Larynx"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"2543","DOI":"10.3389\/fimmu.2019.02543","article-title":"Breast Milk-Derived Extracellular Vesicles Enriched in Exosomes from Mothers with Type 1 Diabetes Contain Aberrant Levels of microRNAs","volume":"10","author":"Mirza","year":"2019","journal-title":"Front. Immunol."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"6650","DOI":"10.3892\/mmr.2017.7462","article-title":"MicroRNA expression profiles and networks in placentas complicated with selective intrauterine growth restriction","volume":"16","author":"Wen","year":"2017","journal-title":"Mol. Med. Rep."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"71","DOI":"10.5830\/CVJA-2016-009","article-title":"Pre-eclampsia: Its pathogenesis and pathophysiolgy","volume":"27","author":"Gathiram","year":"2016","journal-title":"Cardiovasc. J. Afr."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"20065","DOI":"10.1038\/s41598-020-77119-6","article-title":"Potential miRNA biomarkers for the diagnosis and prognosis of esophageal cancer detected by a novel absolute quantitative RT-qPCR method","volume":"10","author":"Lin","year":"2020","journal-title":"Sci. Rep."},{"key":"ref_48","first-page":"934","article-title":"Overview of microRNAs as liquid biopsy biomarkers for colorectal cancer sub-type profiling and chemoresistance","volume":"4","author":"Buhagiar","year":"2021","journal-title":"Cancer Drug Resist"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"18091","DOI":"10.1038\/s41598-019-54574-4","article-title":"6-Bromoindirubin-3\u2032-oxime intercepts GSK3 signaling to promote and enhance skeletal muscle differentiation affecting miR-206 expression in mice","volume":"9","author":"Ragozzino","year":"2019","journal-title":"Sci. Rep."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"1197","DOI":"10.3389\/fmicb.2020.01197","article-title":"MicroRNA Biomarkers for Infectious Diseases: From Basic Research to Biosensing","volume":"11","author":"Tribolet","year":"2020","journal-title":"Front. Microbiol."},{"key":"ref_51","doi-asserted-by":"crossref","unstructured":"Brancaccio, M., Natale, F., Falco, G., and Angrisano, T. (2019). Cell-Free DNA Methylation: The New Frontiers of Pancreatic Cancer Biomarkers\u2019 Discovery. Genes, 11.","DOI":"10.3390\/genes11010014"},{"key":"ref_52","doi-asserted-by":"crossref","unstructured":"Brancaccio, M., Mennitti, C., Calvanese, M., Gentile, A., Musto, R., Gaudiello, G., Scamardella, G., Terracciano, D., Frisso, G., and Pero, R. (2022). Diagnostic and Therapeutic Potential for HNP-1, HBD-1 and HBD-4 in Pregnant Women with COVID-19. Int. J. Mol. Sci., 23.","DOI":"10.3390\/ijms23073450"},{"key":"ref_53","doi-asserted-by":"crossref","unstructured":"Pero, R., Brancaccio, M., Mennitti, C., Gentile, L., Franco, A., Laneri, S., De Biasi, M.G., Pagliuca, C., Colicchio, R., and Salvatore, P. (2020). HNP-1 and HBD-1 as Biomarkers for the Immune Systems of Elite Basketball Athletes. Antibiotics, 9.","DOI":"10.3390\/antibiotics9060306"},{"key":"ref_54","doi-asserted-by":"crossref","unstructured":"Lake, J., Storm, C.S., Makarious, M.B., and Bandres-Ciga, S. (2021). Genetic and Transcriptomic Biomarkers in Neurodegenerative Diseases: Current Situation and the Road Ahead. Cells, 10.","DOI":"10.3390\/cells10051030"},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"586871","DOI":"10.3389\/fcvm.2020.586871","article-title":"Predicting Diagnostic Gene Biomarkers Associated with Immune Infiltration in Patients with Acute Myocardial Infarction","volume":"7","author":"Zhao","year":"2020","journal-title":"Front. Cardiovasc. Med."},{"key":"ref_56","doi-asserted-by":"crossref","unstructured":"Ruggieri, V., Russi, S., Zoppoli, P., La Rocca, F., Angrisano, T., Falco, G., Calice, G., and Laurino, S. (2019). The Role of MicroRNAs in the Regulation of Gastric Cancer Stem Cells: A Meta-Analysis of the Current Status. J. Clin. Med., 8.","DOI":"10.3390\/jcm8050639"},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"2760","DOI":"10.1096\/fj.201601146R","article-title":"Expression and trafficking of placental microRNAs at the feto-maternal interface","volume":"31","author":"Chang","year":"2017","journal-title":"FASEB J."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"646326","DOI":"10.3389\/fcell.2021.646326","article-title":"Placenta-Derived MicroRNAs in the Pathophysiology of Human Pregnancy","volume":"9","author":"Xu","year":"2021","journal-title":"Front. Cell Dev. Biol."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"149","DOI":"10.1016\/j.preghy.2017.07.148","article-title":"Maternal serum IGF-1, IGFBP-1 and 3, and placental growth hormone at 20weeks\u2019 gestation in pregnancies complicated by preeclampsia","volume":"10","author":"Liao","year":"2017","journal-title":"Pregnancy Hypertens"},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"214","DOI":"10.1016\/S0020-7292(03)00342-4","article-title":"Serum levels of insulin, IGF-1, and IGFBP-1 in pre-eclampsia and eclampsia","volume":"84","author":"Ingec","year":"2004","journal-title":"Int. J. Gynaecol. Obstet."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"101","DOI":"10.1530\/EJE-07-0386","article-title":"Changes in circulating level of IGF-I and IGF-binding protein-1 from the first to second trimester as predictors of preeclampsia","volume":"158","author":"Vatten","year":"2008","journal-title":"Eur. J. Endocrinol."},{"key":"ref_62","first-page":"1828","article-title":"Preeclampsia is associated with low circulating levels of insulin-like growth factor I and 1,25-dihydroxyvitamin D in maternal and umbilical cord compartments","volume":"85","author":"Halhali","year":"2000","journal-title":"J. Clin. Endocrinol. Metab."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"108953","DOI":"10.1016\/j.biopha.2019.108953","article-title":"miR-141-5p regulate ATF2 via effecting MAPK1\/ERK2 signaling to promote preeclampsia","volume":"115","author":"Wang","year":"2019","journal-title":"Biomed. Pharmacother."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1016\/j.placenta.2016.05.007","article-title":"FGF1 and FGF2 mutations in preeclampsia and related features","volume":"43","author":"Marwa","year":"2016","journal-title":"Placenta"},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1186\/s13148-018-0472-5","article-title":"Placental surface area mediates the association between FGFR2 methylation in placenta and full-term low birth weight in girls","volume":"22","author":"Tian","year":"2018","journal-title":"Clin. Epigenet."},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"227","DOI":"10.3109\/00016348509155117","article-title":"Correlations between PRL and chloride, sodium, potassium and calcium in amniotic fluid","volume":"64","author":"Andersen","year":"1985","journal-title":"Acta Obstet. Gynecol. Scand."},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"1827","DOI":"10.1016\/S0002-9440(10)63029-5","article-title":"Transforming growth factor-\u03b2 expression in human placenta and placental bed in third trimester normal pregnancy, preeclampsia, and fetal growth restriction","volume":"159","author":"Lyall","year":"2001","journal-title":"Am. J. Pathol."},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1016\/j.semnephrol.2010.10.004","article-title":"Angiogenic factors and preeclampsia","volume":"31","author":"Maynard","year":"2011","journal-title":"Semin. Nephrol."},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"782845","DOI":"10.3389\/fbioe.2021.782845","article-title":"Preeclamptic Women Have Disrupted Placental microRNA Expression at the Time of Preeclampsia Diagnosis: Meta-Analysis","volume":"9","author":"Cirkovic","year":"2021","journal-title":"Front. Bioeng. Biotechnol."}],"container-title":["Genes"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2073-4425\/13\/10\/1781\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T00:45:32Z","timestamp":1760143532000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2073-4425\/13\/10\/1781"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,10,2]]},"references-count":69,"journal-issue":{"issue":"10","published-online":{"date-parts":[[2022,10]]}},"alternative-id":["genes13101781"],"URL":"https:\/\/doi.org\/10.3390\/genes13101781","relation":{},"ISSN":["2073-4425"],"issn-type":[{"value":"2073-4425","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,10,2]]}}}