{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,28]],"date-time":"2026-08-28T15:56:46Z","timestamp":1787932606610,"version":"build-2784847793"},"reference-count":74,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2022,11,24]],"date-time":"2022-11-24T00:00:00Z","timestamp":1669248000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Natural Science Foundation of China","award":["81970519"],"award-info":[{"award-number":["81970519"]}]},{"name":"National Natural Science Foundation of China","award":["81700534"],"award-info":[{"award-number":["81700534"]}]},{"name":"National Natural Science Foundation of China","award":["2017TD-08"],"award-info":[{"award-number":["2017TD-08"]}]},{"DOI":"10.13039\/501100013284","name":"Program for JLU Science and Technology Innovative Research Team","doi-asserted-by":"publisher","award":["81970519"],"award-info":[{"award-number":["81970519"]}],"id":[{"id":"10.13039\/501100013284","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100013284","name":"Program for JLU Science and Technology Innovative Research Team","doi-asserted-by":"publisher","award":["81700534"],"award-info":[{"award-number":["81700534"]}],"id":[{"id":"10.13039\/501100013284","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100013284","name":"Program for JLU Science and Technology Innovative Research Team","doi-asserted-by":"publisher","award":["2017TD-08"],"award-info":[{"award-number":["2017TD-08"]}],"id":[{"id":"10.13039\/501100013284","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Fundamental Research Funds for the Central Universities","award":["81970519"],"award-info":[{"award-number":["81970519"]}]},{"name":"Fundamental Research Funds for the Central Universities","award":["81700534"],"award-info":[{"award-number":["81700534"]}]},{"name":"Fundamental Research Funds for the Central Universities","award":["2017TD-08"],"award-info":[{"award-number":["2017TD-08"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Genes"],"abstract":"<jats:p>MicroRNAs (miRNAs) participate in hepatic stellate cell (HSC) activation, which drives liver fibrosis initiation and progression. We aimed to identify novel hepatic fibrosis targets using miRNA sequencing (miRNA-seq) of human primary HSCs. Surgically resected liver tissues were used to extract HSCs. Based on next-generation sequencing, miRNA-seq was performed on four pairs of HSCs before and after in vitro culture. Additionally, we compared our data with open access miRNA-seq data derived from fourteen cirrhotic and nine healthy liver tissues. Selected miRNAs associated with fibrosis were verified by quantitative real-time PCR. Target mRNAs of differentially expressed (DE) miRNAs were predicted to construct co-expression networks. We identified 230 DEmiRNAs (118 upregulated and 112 downregulated) upon HSC activation. Of the 17 miRNAs with the most significant differences in expression, liver disease-related miRNAs included miR-758-3p, miR-493-5p, miR-409-3p, miR-31-5p, miR-1268a, and miR-381-3p, which might play roles in hepatic fibrosis. Moreover, let-7g-5p, miR-107, miR-122-5p, miR-127-3p, miR-139-5p, miR-148a-3p, miR-194-5p, miR-215-5p, miR-26a-5p, miR-340-5p, miR-451a, and miR-99a-5p were common between our data and the publicly available sequencing data. A co-expression network comprising 1891 matched miRNA\u2013mRNA pairs representing 138 DEmiRNAs and 1414 DEmRNAs was constructed. MiR-1268a and miR-665, possessing the richest target DEmRNAs, may be vital in HSC activation. The targeted genes were involved in collagen metabolism, extracellular matrix structural constituent, cytoskeletal protein binding, and cell adhesion. The miRNAs we identified may provide a basis and reference for the selection of diagnostic and therapeutic targets for hepatic fibrosis.<\/jats:p>","DOI":"10.3390\/genes13122201","type":"journal-article","created":{"date-parts":[[2022,11,24]],"date-time":"2022-11-24T02:54:05Z","timestamp":1669258445000},"page":"2201","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Identification of Liver Fibrosis-Related MicroRNAs in Human Primary Hepatic Stellate Cells Using High-Throughput Sequencing"],"prefix":"10.3390","volume":"13","author":[{"given":"Xu","family":"Liu","sequence":"first","affiliation":[{"name":"Department of Hepatology, First Hospital of Jilin University, Changchun 130021, China"},{"name":"Center of Infectious Diseases and Pathogen Biology, First Hospital of Jilin University, Changchun 130021, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Heming","family":"Ma","sequence":"additional","affiliation":[{"name":"Department of Hepatology, First Hospital of Jilin University, Changchun 130021, China"},{"name":"Center of Infectious Diseases and Pathogen Biology, First Hospital of Jilin University, Changchun 130021, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruihong","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Hepatology, First Hospital of Jilin University, Changchun 130021, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huan","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongqin","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of Hepatology, First Hospital of Jilin University, Changchun 130021, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuxuan","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Hepatobiliary and Pancreatic Surgery, First Hospital of Jilin University, Changchun 130021, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guangyi","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Hepatobiliary and Pancreatic Surgery, First Hospital of Jilin University, Changchun 130021, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guoyue","family":"Lv","sequence":"additional","affiliation":[{"name":"Department of Hepatobiliary and Pancreatic Surgery, First Hospital of Jilin University, Changchun 130021, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junqi","family":"Niu","sequence":"additional","affiliation":[{"name":"Department of Hepatology, First Hospital of Jilin University, Changchun 130021, China"},{"name":"Center of Infectious Diseases and Pathogen Biology, First Hospital of Jilin University, Changchun 130021, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,11,24]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"209","DOI":"10.1172\/JCI24282","article-title":"Liver fibrosis","volume":"115","author":"Bataller","year":"2005","journal-title":"J. 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