{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,22]],"date-time":"2026-06-22T14:49:03Z","timestamp":1782139743792,"version":"3.54.5"},"reference-count":41,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2022,12,2]],"date-time":"2022-12-02T00:00:00Z","timestamp":1669939200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82003551"],"award-info":[{"award-number":["82003551"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["62002086"],"award-info":[{"award-number":["62002086"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81973149"],"award-info":[{"award-number":["81973149"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["2019M661309"],"award-info":[{"award-number":["2019M661309"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["LBH-Z19081"],"award-info":[{"award-number":["LBH-Z19081"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["LBH-Z20064"],"award-info":[{"award-number":["LBH-Z20064"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Chinese Postdoctoral Science Foundation","award":["82003551"],"award-info":[{"award-number":["82003551"]}]},{"name":"Chinese Postdoctoral Science Foundation","award":["62002086"],"award-info":[{"award-number":["62002086"]}]},{"name":"Chinese Postdoctoral Science Foundation","award":["81973149"],"award-info":[{"award-number":["81973149"]}]},{"name":"Chinese Postdoctoral Science Foundation","award":["2019M661309"],"award-info":[{"award-number":["2019M661309"]}]},{"name":"Chinese Postdoctoral Science Foundation","award":["LBH-Z19081"],"award-info":[{"award-number":["LBH-Z19081"]}]},{"name":"Chinese Postdoctoral Science Foundation","award":["LBH-Z20064"],"award-info":[{"award-number":["LBH-Z20064"]}]},{"name":"Heilongjiang Postdoctoral Science Foundation","award":["82003551"],"award-info":[{"award-number":["82003551"]}]},{"name":"Heilongjiang Postdoctoral Science Foundation","award":["62002086"],"award-info":[{"award-number":["62002086"]}]},{"name":"Heilongjiang Postdoctoral Science Foundation","award":["81973149"],"award-info":[{"award-number":["81973149"]}]},{"name":"Heilongjiang Postdoctoral Science Foundation","award":["2019M661309"],"award-info":[{"award-number":["2019M661309"]}]},{"name":"Heilongjiang Postdoctoral Science Foundation","award":["LBH-Z19081"],"award-info":[{"award-number":["LBH-Z19081"]}]},{"name":"Heilongjiang Postdoctoral Science Foundation","award":["LBH-Z20064"],"award-info":[{"award-number":["LBH-Z20064"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Genes"],"abstract":"<jats:p>Epithelial ovarian cancer (EOC) is the main cause of mortality among gynecological malignancies worldwide. Although patients with EOC undergo aggregate treatment, the prognosis is often poor. Peritoneal malignant ascites is a distinguishable clinical feature in EOC patients and plays a pivotal role in tumor progression and recurrence. The mechanisms of the tumor microenvironment (TME) in ascites in the regulation of tumor progression need to be explored. We comprehensively analyzed the transcriptomes of 4680 single cells from five EOC patients (three diagnostic samples and two recurrent samples) derived from Gene Expression Omnibus (GEO) databases. Batch effects between different samples were removed using an unsupervised deep embedding single-cell cluster algorithm. Subcluster analysis identified the different phenotypes of cells. The transition of a malignant cell state was confirmed using pseudotime analysis. The landscape of TME in malignant ascites was profiled during EOC progression. The transformation of epithelial cancer cells into mesenchymal cells was observed to lead to the emergence of related anti-chemotherapy and immune escape phenotypes. We found the activation of multiple biological pathways with the transition of tumor-associated macrophages and fibroblasts, and we identified the infiltration of CD4+CD25+ T regulatory cells in recurrent samples. The cell adhesion molecules mediated by integrin might be associated with the formation of the tumorsphere. Our study provides novel insights into the remodeling of the TME heterogeneity in malignant ascites during EOC progression, which provides evidence for identifying novel therapeutic targets and promotes the development of ovarian cancer treatment.<\/jats:p>","DOI":"10.3390\/genes13122276","type":"journal-article","created":{"date-parts":[[2022,12,5]],"date-time":"2022-12-05T03:38:38Z","timestamp":1670211518000},"page":"2276","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Single-Cell Sequencing of Malignant Ascites Reveals Transcriptomic Remodeling of the Tumor Microenvironment during the Progression of Epithelial Ovarian Cancer"],"prefix":"10.3390","volume":"13","author":[{"given":"Yiqun","family":"Li","sequence":"first","affiliation":[{"name":"School of Life Sciences and Technology, Harbin Institute of Technology, Harbin 150000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenjie","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Biostatistics, School of Public Health, Harbin Medical University, Harbin 150000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Danyun","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Gynecology, Women\u2019s Hospital, School of Medicine, Zhejiang University, Hangzhou 360000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liuchao","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Biostatistics, School of Public Health, Harbin Medical University, Harbin 150000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xizhi","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Biostatistics, School of Public Health, Harbin Medical University, Harbin 150000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jia","family":"He","sequence":"additional","affiliation":[{"name":"Department of Biostatistics, School of Public Health, Harbin Medical University, Harbin 150000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lei","family":"Cao","sequence":"additional","affiliation":[{"name":"Department of Biostatistics, School of Public Health, Harbin Medical University, Harbin 150000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kang","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Biostatistics, School of Public Health, Harbin Medical University, Harbin 150000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5752-9066","authenticated-orcid":false,"given":"Hongyu","family":"Xie","sequence":"additional","affiliation":[{"name":"Clinical Research Center, Women\u2019s Hospital, School of Medicine, Zhejiang University, Hangzhou 360000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,12,2]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"284","DOI":"10.3322\/caac.21456","article-title":"Ovarian cancer statistics, 2018","volume":"68","author":"Torre","year":"2018","journal-title":"CA A Cancer J. 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