{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,28]],"date-time":"2026-02-28T04:23:59Z","timestamp":1772252639970,"version":"3.50.1"},"reference-count":67,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2023,1,8]],"date-time":"2023-01-08T00:00:00Z","timestamp":1673136000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000024","name":"Canadian Institutes of Health Research","doi-asserted-by":"publisher","award":["PJT-166109"],"award-info":[{"award-number":["PJT-166109"]}],"id":[{"id":"10.13039\/501100000024","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Research Center on Aging at Sherbrooke","award":["PJT-166109"],"award-info":[{"award-number":["PJT-166109"]}]},{"name":"Fonds de Recherche du Qu\u00e9bec-Sant\u00e9","award":["PJT-166109"],"award-info":[{"award-number":["PJT-166109"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Genes"],"abstract":"<jats:p>Most pseudogenes are generated when an RNA transcript is reverse-transcribed and integrated into the genome at a new location. Pseudogenes are often considered as an imperfect and silent copy of a functional gene because of the accumulation of numerous mutations in their sequence. Here we report the presence of Pfh8-ps, a Phf8 retrotransposed pseudogene in the mouse genome, which has no disruptions in its coding sequence. We show that this pseudogene is mainly transcribed in testis and can produce a PHF8-PS protein in vivo. As the PHF8-PS protein has a well-conserved JmjC domain, we characterized its enzymatic activity and show that PHF8-PS does not have the intrinsic capability to demethylate H3K9me2 in vitro compared to the parental PHF8 protein. Surprisingly, PHF8-PS does not localize in the nucleus like PHF8, but rather is mostly located at the cytoplasm. Finally, our proteomic analysis of PHF8-PS-associated proteins revealed that PHF8-PS interacts not only with mitochondrial proteins, but also with prefoldin subunits (PFDN proteins) that deliver unfolded proteins to the cytosolic chaperonin complex implicated in the folding of cytosolic proteins. Together, our findings highlighted PHF8-PS as a new pseudogene-derived protein with distinct molecular functions from PHF8.<\/jats:p>","DOI":"10.3390\/genes14010172","type":"journal-article","created":{"date-parts":[[2023,1,9]],"date-time":"2023-01-09T02:31:30Z","timestamp":1673231490000},"page":"172","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Functional Characterization of a Phf8 Processed Pseudogene in the Mouse Genome"],"prefix":"10.3390","volume":"14","author":[{"given":"Joannie","family":"St-Germain","sequence":"first","affiliation":[{"name":"Research Center on Aging, Centre Int\u00e9gr\u00e9 Universitaire de Sant\u00e9 et Services Sociaux de l\u2019Estrie-Centre Hospitalier Universitaire de Sherbrooke, Sherbrooke, QC J1H 4C4, Canada"},{"name":"Department of Biochemistry and Functional Genomics, Faculty of Medicine and Health Sciences, Universit\u00e9 de Sherbrooke, Sherbrooke, QC J1H 5N4, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Muhammad Riaz","family":"Khan","sequence":"additional","affiliation":[{"name":"Research Center on Aging, Centre Int\u00e9gr\u00e9 Universitaire de Sant\u00e9 et Services Sociaux de l\u2019Estrie-Centre Hospitalier Universitaire de Sherbrooke, Sherbrooke, QC J1H 4C4, Canada"},{"name":"Department of Biochemistry and Functional Genomics, Faculty of Medicine and Health Sciences, Universit\u00e9 de Sherbrooke, Sherbrooke, QC J1H 5N4, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Viktoriia","family":"Bavykina","sequence":"additional","affiliation":[{"name":"Research Center on Aging, Centre Int\u00e9gr\u00e9 Universitaire de Sant\u00e9 et Services Sociaux de l\u2019Estrie-Centre Hospitalier Universitaire de Sherbrooke, Sherbrooke, QC J1H 4C4, Canada"},{"name":"Department of Biochemistry and Functional Genomics, Faculty of Medicine and Health Sciences, Universit\u00e9 de Sherbrooke, Sherbrooke, QC J1H 5N4, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rebecka","family":"Desmarais","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Functional Genomics, Faculty of Medicine and Health Sciences, Universit\u00e9 de Sherbrooke, Sherbrooke, QC J1H 5N4, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Micah","family":"Scott","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Functional Genomics, Faculty of Medicine and Health Sciences, Universit\u00e9 de Sherbrooke, Sherbrooke, QC J1H 5N4, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guylain","family":"Boissonneault","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Functional Genomics, Faculty of Medicine and Health Sciences, Universit\u00e9 de Sherbrooke, Sherbrooke, QC J1H 5N4, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5973-3522","authenticated-orcid":false,"given":"Marie A.","family":"Brunet","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, Medical Genetics Service, Universit\u00e9 de Sherbrooke, Sherbrooke, QC J1H 5N4, Canada"},{"name":"Centre de Recherche du Centre Hospitalier Universitaire de Sherbrooke (CRCHUS), Sherbrooke, QC J1H 5N4, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4032-3213","authenticated-orcid":false,"given":"Benoit","family":"Laurent","sequence":"additional","affiliation":[{"name":"Research Center on Aging, Centre Int\u00e9gr\u00e9 Universitaire de Sant\u00e9 et Services Sociaux de l\u2019Estrie-Centre Hospitalier Universitaire de Sherbrooke, Sherbrooke, QC J1H 4C4, Canada"},{"name":"Department of Biochemistry and Functional Genomics, Faculty of Medicine and Health Sciences, Universit\u00e9 de Sherbrooke, Sherbrooke, QC J1H 5N4, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2023,1,8]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1016\/0092-8674(77)90189-1","article-title":"A pseudogene structure in 5S DNA of Xenopus laevis","volume":"12","author":"Jacq","year":"1977","journal-title":"Cell"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1016\/S0014-5793(00)01199-6","article-title":"Vertebrate pseudogenes","volume":"468","author":"Mighell","year":"2000","journal-title":"FEBS Lett."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"191","DOI":"10.1038\/s41576-019-0196-1","article-title":"Overcoming challenges and dogmas to understand the functions of pseudogenes","volume":"21","author":"Cheetham","year":"2020","journal-title":"Nat. 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