{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T13:57:50Z","timestamp":1790171870613,"version":"4.1.0"},"reference-count":53,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2023,1,21]],"date-time":"2023-01-21T00:00:00Z","timestamp":1674259200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Czech Academy of Sciences","award":["765269"],"award-info":[{"award-number":["765269"]}]},{"name":"NGO \u201cAssociation of Gene Therapy (ASGENT)","award":["765269"],"award-info":[{"award-number":["765269"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Genes"],"abstract":"<jats:p>Stress responses are activated by the hypothalamic-pituitary-adrenal axis (HPA axis), culminating in the release of glucocorticoids. During prolonged periods of secretion of glucocorticoids or inappropriate behavioral responses to a stressor, pathologic conditions may occur. Increased glucocorticoid concentration is linked to generalized anxiety, and there are knowledge gaps regarding its regulation. It is known that the HPA axis is under GABAergic control, but the contribution of the individual subunits of the GABA receptor is largely unknown. In this study, we investigated the relationship between the \u03b15 subunit and corticosterone levels in a new mouse model deficient for Gabra5, which is known to be linked to anxiety disorders in humans and phenologs observed in mice. We observed decreased rearing behavior, suggesting lower anxiety in the Gabra5\u2212\/\u2212 animals; however, such a phenotype was absent in the open field and elevated plus maze tests. In addition to decreased rearing behavior, we also found decreased levels of fecal corticosterone metabolites in Gabra5\u2212\/\u2212 mice indicating a lowered stress response. Moreover, based on the electrophysiological recordings where we observed a hyperpolarized state of hippocampal neurons, we hypothesize that the constitutive ablation of the Gabra5 gene leads to functional compensation with other channels or GABA receptor subunits in this model.<\/jats:p>","DOI":"10.3390\/genes14020285","type":"journal-article","created":{"date-parts":[[2023,1,25]],"date-time":"2023-01-25T03:21:54Z","timestamp":1674616914000},"page":"285","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Ablation of Gabra5 Influences Corticosterone Levels and Anxiety-like Behavior in Mice"],"prefix":"10.3390","volume":"14","author":[{"given":"Linn Amanda","family":"Syding","sequence":"first","affiliation":[{"name":"Laboratory of Transgenic Models of Diseases, Institute of Molecular Genetics of the CAS, 25250 Vestec, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Agnieszka","family":"Kubik-Zahorodna","sequence":"additional","affiliation":[{"name":"Czech Centre for Phenogenomics, Institute of Molecular Genetics of the CAS, 25250 Vestec, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7715-9402","authenticated-orcid":false,"given":"David Pajuelo","family":"Reguera","sequence":"additional","affiliation":[{"name":"Czech Centre for Phenogenomics, Institute of Molecular Genetics of the CAS, 25250 Vestec, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4289-0502","authenticated-orcid":false,"given":"Petr","family":"Nickl","sequence":"additional","affiliation":[{"name":"Laboratory of Transgenic Models of Diseases, Institute of Molecular Genetics of the CAS, 25250 Vestec, Czech Republic"},{"name":"Czech Centre for Phenogenomics, Institute of Molecular Genetics of the CAS, 25250 Vestec, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bohdana","family":"Hruskova","sequence":"additional","affiliation":[{"name":"Department of Auditory Neuroscience, Institute of Experimental Medicine of the Czech Academy of Sciences, 14220 Prague, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michaela","family":"Kralikova","sequence":"additional","affiliation":[{"name":"Department of Auditory Neuroscience, Institute of Experimental Medicine of the Czech Academy of Sciences, 14220 Prague, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jana","family":"Kopkanova","sequence":"additional","affiliation":[{"name":"Czech Centre for Phenogenomics, Institute of Molecular Genetics of the CAS, 25250 Vestec, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vendula","family":"Novosadova","sequence":"additional","affiliation":[{"name":"Czech Centre for Phenogenomics, Institute of Molecular Genetics of the CAS, 25250 Vestec, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Petr","family":"Kasparek","sequence":"additional","affiliation":[{"name":"Czech Centre for Phenogenomics, Institute of Molecular Genetics of the CAS, 25250 Vestec, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jan","family":"Prochazka","sequence":"additional","affiliation":[{"name":"Laboratory of Transgenic Models of Diseases, Institute of Molecular Genetics of the CAS, 25250 Vestec, Czech Republic"},{"name":"Czech Centre for Phenogenomics, Institute of Molecular Genetics of the CAS, 25250 Vestec, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8035-8904","authenticated-orcid":false,"given":"Jan","family":"Rozman","sequence":"additional","affiliation":[{"name":"Czech Centre for Phenogenomics, Institute of Molecular Genetics of the CAS, 25250 Vestec, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rostislav","family":"Turecek","sequence":"additional","affiliation":[{"name":"Department of Auditory Neuroscience, Institute of Experimental Medicine of the Czech Academy of Sciences, 14220 Prague, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3352-392X","authenticated-orcid":false,"given":"Radislav","family":"Sedlacek","sequence":"additional","affiliation":[{"name":"Laboratory of Transgenic Models of Diseases, Institute of Molecular Genetics of the CAS, 25250 Vestec, Czech Republic"},{"name":"Czech Centre for Phenogenomics, Institute of Molecular Genetics of the CAS, 25250 Vestec, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,1,21]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"222","DOI":"10.3389\/fgene.2012.00222","article-title":"Neuronal Responses to Physiological Stress","volume":"3","author":"Kagias","year":"2012","journal-title":"Front. Genet."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1111\/nuf.12044","article-title":"Stress: A concept analysis","volume":"49","author":"Goodnite","year":"2014","journal-title":"Nurs. Forum"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"S627","DOI":"10.4103\/2230-8210.105581","article-title":"PVN pathways controlling energy homeostasis","volume":"16","author":"Hill","year":"2012","journal-title":"Indian J. Endocrinol. Metab."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"383","DOI":"10.31887\/DCNS.2006.8.4\/ssmith","article-title":"The role of the hypothalamic-pituitary-adrenal axis in neuroendocrine responses to stress","volume":"8","author":"Smith","year":"2006","journal-title":"Dialogues Clin. Neurosci."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"3022","DOI":"10.1016\/j.celrep.2017.11.053","article-title":"Glucocorticoid Receptor Binding Induces Rapid and Prolonged Large-Scale Chromatin Decompaction at Multiple Target Loci","volume":"21","author":"Jubb","year":"2017","journal-title":"Cell Rep."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"R1","DOI":"10.1530\/EC-16-0100","article-title":"The role of glucocorticoids and corticotropin-releasing hormone regulation on anxiety symptoms and response to treatment","volume":"6","author":"Raglan","year":"2017","journal-title":"Endocr. Connect."},{"key":"ref_7","unstructured":"Sapolsky, R.M. (2004). Why Zebras Don\u2019t Get Ulcers: The Acclaimed Guide to Stress, Stress-Related Diseases, and Coping, Holt Paperbacks."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1007\/s00429-008-0192-2","article-title":"Functional role of local GABAergic influences on the HPA axis","volume":"213","author":"Cullinan","year":"2008","journal-title":"Brain Struct. Funct."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"131","DOI":"10.3389\/fnins.2011.00131","article-title":"Neurosteroids and GABAA Receptor Interactions: A Focus on Stress","volume":"5","author":"Gunn","year":"2011","journal-title":"Front. Neurosci."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"88","DOI":"10.1002\/(SICI)1096-9861(19960422)368:1<88::AID-CNE6>3.0.CO;2-G","article-title":"Fos expression in forebrain afferents to the hypothalamic paraventricular nucleus following swim stress","volume":"368","author":"Cullinan","year":"1996","journal-title":"J. Comp. Neurol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"18","DOI":"10.3389\/fnmol.2018.00018","article-title":"Molecular Characterization of GABA-A Receptor Subunit Diversity within Major Peripheral Organs and Their Plasticity in Response to Early Life Psychosocial Stress","volume":"11","author":"Everington","year":"2018","journal-title":"Front. Mol. Neurosci."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"179","DOI":"10.3389\/fnmol.2019.00179","article-title":"Neurobiology and Therapeutic Potential of \u03b15-GABA Type A Receptors","volume":"12","author":"Jacob","year":"2019","journal-title":"Front. Mol. Neurosci."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"18198","DOI":"10.1523\/JNEUROSCI.2560-11.2011","article-title":"Neurosteroidogenesis is required for the physiological response to stress: Role of neurosteroid-sensitive GABAA receptors","volume":"31","author":"Sarkar","year":"2011","journal-title":"J. Neurosci."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"93","DOI":"10.2147\/TACG.S57386","article-title":"Angelman syndrome: Review of clinical and molecular aspects","volume":"7","author":"Bird","year":"2014","journal-title":"Appl. Clin. Genet."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"2423","DOI":"10.1038\/npp.2014.92","article-title":"Evidence for linkage and association of GABRB3 and GABRA5 to panic disorder","volume":"39","author":"Hodges","year":"2014","journal-title":"Neuropsychopharmacology"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"788","DOI":"10.1523\/JNEUROSCI.0567-18.2018","article-title":"Input-Specific Synaptic Location and Function of the \u03b15 GABAA Receptor Subunit in the Mouse CA1 Hippocampal Neurons","volume":"39","author":"Magnin","year":"2019","journal-title":"J. Neurosci."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"1389","DOI":"10.1016\/S0278-5846(02)00305-6","article-title":"Anxiogenic-like activity of L-655,708, a selective ligand for the benzodiazepine site of GABA(A) receptors which contain the \u03b1-5 subunit, in the elevated plus-maze test","volume":"26","author":"Navarro","year":"2002","journal-title":"Prog. Neuro Psychopharmacol. Biol. Psychiatry"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"2492","DOI":"10.1038\/npp.2016.49","article-title":"A Pharmacogenetic \u2018Restriction-of-Function\u2019 Approach Reveals Evidence for Anxiolytic-Like Actions Mediated by \u03b15-Containing GABAA Receptors in Mice","volume":"41","author":"Behlke","year":"2016","journal-title":"Neuropsychopharmacology"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"141","DOI":"10.1016\/j.neuropharm.2008.07.045","article-title":"GABA A receptors: Subtypes provide diversity of function and pharmacology","volume":"56","author":"Olsen","year":"2009","journal-title":"Neuropharmacology"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"180","DOI":"10.1016\/j.yfrne.2013.12.003","article-title":"Glucocorticoid actions on synapses, circuits, and behavior: Implications for the energetics of stress","volume":"35","author":"Myers","year":"2014","journal-title":"Front. Neuroendocrinol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"1334","DOI":"10.1042\/BST0371334","article-title":"The physiological properties and therapeutic potential of alpha5-GABAA receptors","volume":"37","author":"Martin","year":"2009","journal-title":"Biochem. Soc. Trans."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"169","DOI":"10.1038\/npp.2009.83","article-title":"The neurocircuitry of fear, stress, and anxiety disorders","volume":"35","author":"Shin","year":"2010","journal-title":"Neuropsychopharmacol. Off. Publ. Am. Coll. Neuropsychopharmacol."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"603","DOI":"10.1002\/cphy.c150015","article-title":"Regulation of the Hypothalamic-Pituitary-Adrenocortical Stress Response","volume":"6","author":"Herman","year":"2016","journal-title":"Compr. Physiol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"273","DOI":"10.3389\/fnbeh.2014.00273","article-title":"The role of the hippocampus in avoidance learning and anxiety vulnerability","volume":"8","author":"Cominski","year":"2014","journal-title":"Front. Behav. Neurosci."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"670","DOI":"10.1016\/j.neuron.2018.01.016","article-title":"Anxiety Cells in a Hippocampal-Hypothalamic Circuit","volume":"97","author":"Jimenez","year":"2018","journal-title":"Neuron"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"3662","DOI":"10.1073\/pnas.0307231101","article-title":"Tonic inhibition in mouse hippocampal CA1 pyramidal neurons is mediated by alpha5 subunit-containing \u03b3-aminobutyric acid type A receptors","volume":"101","author":"Caraiscos","year":"2004","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"5572","DOI":"10.1523\/JNEUROSCI.22-13-05572.2002","article-title":"Enhanced learning and memory and altered GABAergic synaptic transmission in mice lacking the \u03b1 5 subunit of the GABAA receptor","volume":"22","author":"Collinson","year":"2002","journal-title":"J. Neurosci."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"30","DOI":"10.1016\/j.physbeh.2014.05.006","article-title":"Assessment of mouse anxiety-like behavior in the light-dark box and open-field arena: Role of equipment and procedure","volume":"133","author":"Kulesskaya","year":"2014","journal-title":"Physiol. Behav."},{"key":"ref_29","first-page":"e50871","article-title":"Contextual and cued fear conditioning test using a video analyzing system in mice","volume":"85","author":"Shoji","year":"2014","journal-title":"J. Vis. Exp."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"5269","DOI":"10.1523\/JNEUROSCI.4209-09.2010","article-title":"\u03b15GABAAReceptor Activity Sets the Threshold for Long-Term Potentiation and Constrains Hippocampus-Dependent Memory","volume":"30","author":"Martin","year":"2010","journal-title":"J. Neurosci."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"2796","DOI":"10.1152\/jn.01122.2005","article-title":"Hippocampal network hyperactivity after selective reduction of tonic inhibition in GABA A receptor alpha5 subunit-deficient mice","volume":"95","author":"Glykys","year":"2006","journal-title":"J. Neurophysiol."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"F115","DOI":"10.1152\/ajprenal.00377.2019","article-title":"A noninvasive method to study the evolution of extracellular fluid volume in mice using time-domain nuclear magnetic resonance","volume":"319","author":"Morla","year":"2020","journal-title":"Am. J. Physiol. Ren. Physiol."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"5573","DOI":"10.1073\/pnas.0705615105","article-title":"Acute corticosterone treatment is sufficient to induce anxiety and amygdaloid dendritic hypertrophy","volume":"105","author":"Mitra","year":"2008","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"443","DOI":"10.1080\/10253890.2018.1438405","article-title":"Exploratory rearing: A context- and stress-sensitive behavior recorded in the open-field test","volume":"21","author":"Sturman","year":"2018","journal-title":"Stress"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"100972","DOI":"10.1016\/j.molmet.2020.02.015","article-title":"Effects of corticosterone within the hypothalamic arcuate nucleus on food intake and body weight in male rats","volume":"36","author":"Ma","year":"2020","journal-title":"Mol. Metab."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"458","DOI":"10.1002\/cne.21115","article-title":"Synaptic and nonsynaptic localization of GABAA receptors containing the alpha5 subunit in the rat brain","volume":"499","author":"Serwanski","year":"2006","journal-title":"J. Comp. Neurol."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"2244","DOI":"10.1152\/jn.00482.2007","article-title":"Alpha5GABAA receptors regulate the intrinsic excitability of mouse hippocampal pyramidal neurons","volume":"98","author":"Bonin","year":"2007","journal-title":"J. Neurophysiol."},{"key":"ref_38","doi-asserted-by":"crossref","unstructured":"Syding, L.A., Nickl, P., Kasparek, P., and Sedlacek, R. (2020). CRISPR\/Cas9 Epigenome Editing Potential for Rare Imprinting Diseases: A Review. Cells, 9.","DOI":"10.3390\/cells9040993"},{"key":"ref_39","unstructured":"Pfaff, D.W., and Joels, M. (2009). Hormones, Brain and Behavior, Academic Press. [2nd ed.]."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"15211","DOI":"10.1038\/s41598-019-51674-z","article-title":"Cage aggression in group-housed laboratory male mice: An international data crowdsourcing project","volume":"9","author":"Lidster","year":"2019","journal-title":"Sci. Rep."},{"key":"ref_41","first-page":"RA136","article-title":"Testosterone and aggressiveness","volume":"11","author":"Giammanco","year":"2005","journal-title":"Med. Sci. Monit. Int. Med. J. Exp. Clin. Res."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"446","DOI":"10.3389\/fphar.2016.00446","article-title":"Sex-Dependent Anti-Stress Effect of an \u03b15 Subunit Containing GABAA Receptor Positive Allosteric Modulator","volume":"7","author":"Piantadosi","year":"2016","journal-title":"Front. Pharmacol."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"111","DOI":"10.1515\/REVNEURO.2006.17.1-2.111","article-title":"Rearing on hind legs, environmental novelty, and the hippocampal formation","volume":"17","author":"Lever","year":"2006","journal-title":"Rev. Neurosci."},{"key":"ref_44","doi-asserted-by":"crossref","unstructured":"Olivier, B., Mos, J., and Slangen, J.L. (1991). Animal Models in Psychopharmacology, Birkh\u00e4user Basel.","DOI":"10.1007\/978-3-0348-6419-0"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"1129","DOI":"10.1111\/j.1476-5381.2011.01362.x","article-title":"The age of anxiety: Role of animal models of anxiolytic action in drug discovery","volume":"164","author":"Cryan","year":"2011","journal-title":"Br. J. Pharmacol."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"367","DOI":"10.1007\/978-981-32-9705-0_20","article-title":"Role of Benzodiazepines in Anxiety Disorders","volume":"1191","author":"Balon","year":"2020","journal-title":"Anxiety Disord."},{"key":"ref_47","doi-asserted-by":"crossref","unstructured":"El-Brolosy, M.A., and Stainier, D.Y.R. (2017). Genetic compensation: A phenomenon in search of mechanisms. PLoS Genet., 13.","DOI":"10.1371\/journal.pgen.1006780"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"8980","DOI":"10.1073\/pnas.142288699","article-title":"Trace fear conditioning involves hippocampal alpha5 GABA(A) receptors","volume":"99","author":"Crestani","year":"2002","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"1025","DOI":"10.1097\/ALN.0b013e3181bbc961","article-title":"Etomidate targets alpha5 \u03b3-aminobutyric acid subtype A receptors to regulate synaptic plasticity and memory blockade","volume":"111","author":"Martin","year":"2009","journal-title":"Anesthesiology"},{"key":"ref_50","doi-asserted-by":"crossref","unstructured":"Bonin, R.P., Zurek, A.A., Yu, J., Bayliss, D.A., and Orser, B.A. (2013). Hyperpolarization-Activated Current (Ih) Is Reduced in Hippocampal Neurons from Gabra5\u2212\/\u2212 Mice. PLoS ONE, 8.","DOI":"10.1371\/journal.pone.0058679"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"1421","DOI":"10.1523\/JNEUROSCI.4751-07.2008","article-title":"Which GABA(A) receptor subunits are necessary for tonic inhibition in the hippocampus?","volume":"28","author":"Glykys","year":"2008","journal-title":"J. Neurosci."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"88","DOI":"10.1038\/35051086","article-title":"Adaptive regulation of neuronal excitability by a voltage-independent potassium conductance","volume":"409","author":"Brickley","year":"2001","journal-title":"Nature"},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"614435","DOI":"10.3389\/fnmol.2020.614435","article-title":"The Neomycin Resistance Cassette in the Targeted Allele of Shank3B Knock-Out Mice Has Potential Off-Target Effects to Produce an Unusual Shank3 Isoform","volume":"13","author":"Jin","year":"2021","journal-title":"Front. Mol. Neurosci."}],"container-title":["Genes"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2073-4425\/14\/2\/285\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T18:13:07Z","timestamp":1760119987000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2073-4425\/14\/2\/285"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,1,21]]},"references-count":53,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2023,2]]}},"alternative-id":["genes14020285"],"URL":"https:\/\/doi.org\/10.3390\/genes14020285","relation":{},"ISSN":["2073-4425"],"issn-type":[{"value":"2073-4425","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,1,21]]}}}