{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T17:22:11Z","timestamp":1787160131557,"version":"build-2736575974"},"reference-count":82,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2023,4,6]],"date-time":"2023-04-06T00:00:00Z","timestamp":1680739200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Genes"],"abstract":"<jats:p>Antimicrobial-resistant Escherichia coli isolates have emerged in various ecologic compartments and evolved to spread globally. We sought to (1.) investigate the occurrence of ESBL-producing E. coli (ESBL-Ec) in feces from free-range chickens in a rural region and (2.) characterize the genetic background of antimicrobial resistance and the genetic relatedness of collected isolates. Ninety-five feces swabs from free-range chickens associated with two households (House 1\/House 2) in a rural region in northern Tunisia were collected. Samples were screened to recover ESBL-Ec, and collected isolates were characterized for phenotype\/genotype of antimicrobial resistance, integrons, and molecular typing (pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST)). Overall, 47 ESBL-Ec were identified, with the following genes detected: 35 blaCTX-M-1, 5 blaCTX-M-55, 5 blaCTX-M-15, 1 blaSHV-2, and 1 blaSHV-12. Resistance to fluoroquinolones, tetracycline, sulfonamides, and colistin was encoded by aac(6\u2032)-Ib-cr (n = 21), qnrB (n = 1), and qnrS (n = 2); tetA (n = 17)\/tetB (n = 26); sul1 (n = 29)\/sul2 (n = 18); and mcr-2 (n = 2) genes, respectively. PFGE and MLST identified genetic homogeneity of isolates in House 1; however, isolates from House 2 were heterogeneous. Notably, among nine identified sequence types, ST58, ST69, ST224, and ST410 belong to pandemic high-risk clonal lineages associated with extrapathogenic E. coli. Minor clones belonging to ST410 and ST471 were shared by chickens from both households. The virulence genes fyuA, fimH, papGIII, and iutA were detected in 35, 47, 17, and 23 isolates, respectively. Findings indicate a high occurrence of ESBL-Ec in free-range chickens and highlight the occurrence of pandemic zoonotic clones.<\/jats:p>","DOI":"10.3390\/genes14040875","type":"journal-article","created":{"date-parts":[[2023,4,7]],"date-time":"2023-04-07T04:08:03Z","timestamp":1680840483000},"page":"875","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":21,"title":["Occurrence of High-Risk Clonal Lineages ST58, ST69, ST224, and ST410 among Extended-Spectrum \u03b2-Lactamase-Producing Escherichia coli Isolated from Healthy Free-Range Chickens (Gallus gallus domesticus) in a Rural Region in Tunisia"],"prefix":"10.3390","volume":"14","author":[{"given":"Saloua","family":"Benlabidi","sequence":"first","affiliation":[{"name":"Institute of Veterinary Research of Tunisia, University of Tunis El Manar, Tunis 1006, Tunisia"},{"name":"Laboratory of Neurophysiology Cellular Physiopathology and Biomolecule Valorisation LR18ES03, Faculty of Sciences of Tunis, University Tunis El Manar, Tunis 1068, Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0293-9735","authenticated-orcid":false,"given":"Anis","family":"Raddaoui","sequence":"additional","affiliation":[{"name":"Laboratory Ward, National Bone Marrow Transplant Center, Tunis 1006, Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sana","family":"Lengliz","sequence":"additional","affiliation":[{"name":"Institute of Veterinary Research of Tunisia, University of Tunis El Manar, Tunis 1006, Tunisia"},{"name":"Laboratory of Materials, Molecules and Application LR11ES22, Preparatory Institute for Scientific and Technical Studies, University of Carthage, Tunis 1054, Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sarah","family":"Cheriet","sequence":"additional","affiliation":[{"name":"Institute of Veterinary Research of Tunisia, University of Tunis El Manar, Tunis 1006, Tunisia"},{"name":"Laboratory of Neurophysiology Cellular Physiopathology and Biomolecule Valorisation LR18ES03, Faculty of Sciences of Tunis, University Tunis El Manar, Tunis 1068, Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1260-2688","authenticated-orcid":false,"given":"Paul","family":"Hynds","sequence":"additional","affiliation":[{"name":"Environmental Sustainability and Health Institute (ESHI), Technological University Dublin, D07 H6K8 Dublin, Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7245-9190","authenticated-orcid":false,"given":"Wafa","family":"Achour","sequence":"additional","affiliation":[{"name":"Laboratory Ward, National Bone Marrow Transplant Center, Tunis 1006, Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Taoufik","family":"Ghrairi","sequence":"additional","affiliation":[{"name":"Laboratory of Neurophysiology Cellular Physiopathology and Biomolecule Valorisation LR18ES03, Faculty of Sciences of Tunis, University Tunis El Manar, Tunis 1068, Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9673-193X","authenticated-orcid":false,"given":"Mohamed","family":"Abbassi","sequence":"additional","affiliation":[{"name":"Institute of Veterinary Research of Tunisia, University of Tunis El Manar, Tunis 1006, Tunisia"},{"name":"Research Laboratory \u2018Antimicrobial Resistance\u2019 LR18ES39, Faculty of Medicine of Tunis, University of Tunis El Manar, Tunis 1006, Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,4,6]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"fiac045","DOI":"10.1093\/femsec\/fiac045","article-title":"Hiding in plain sight-wildlife as a neglected reservoir and pathway for the spread of antimicrobial resistance: A narrative review","volume":"98","author":"Abbassi","year":"2022","journal-title":"FEMS Microbiol. 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