{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,23]],"date-time":"2026-07-23T18:07:09Z","timestamp":1784830029661,"version":"3.55.0"},"reference-count":37,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2023,4,10]],"date-time":"2023-04-10T00:00:00Z","timestamp":1681084800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Key R&amp;D Program","award":["2021YFD1300901"],"award-info":[{"award-number":["2021YFD1300901"]}]},{"name":"National Key R&amp;D Program","award":["2017YFD0501901"],"award-info":[{"award-number":["2017YFD0501901"]}]},{"name":"National Key R&amp;D Program","award":["2017YFD0501905"],"award-info":[{"award-number":["2017YFD0501905"]}]},{"name":"National Key R&amp;D Program","award":["31930103"],"award-info":[{"award-number":["31930103"]}]},{"name":"National Key R&amp;D Program","award":["3167246"],"award-info":[{"award-number":["3167246"]}]},{"name":"National Key R&amp;D Program","award":["BAIC05-2022"],"award-info":[{"award-number":["BAIC05-2022"]}]},{"name":"National Natural Science Foundation of China","award":["2021YFD1300901"],"award-info":[{"award-number":["2021YFD1300901"]}]},{"name":"National Natural Science Foundation of China","award":["2017YFD0501901"],"award-info":[{"award-number":["2017YFD0501901"]}]},{"name":"National Natural Science Foundation of China","award":["2017YFD0501905"],"award-info":[{"award-number":["2017YFD0501905"]}]},{"name":"National Natural Science Foundation of China","award":["31930103"],"award-info":[{"award-number":["31930103"]}]},{"name":"National Natural Science Foundation of China","award":["3167246"],"award-info":[{"award-number":["3167246"]}]},{"name":"National Natural Science Foundation of China","award":["BAIC05-2022"],"award-info":[{"award-number":["BAIC05-2022"]}]},{"name":"Beijing Innovation Consortium of Livestock Research System","award":["2021YFD1300901"],"award-info":[{"award-number":["2021YFD1300901"]}]},{"name":"Beijing Innovation Consortium of Livestock Research System","award":["2017YFD0501901"],"award-info":[{"award-number":["2017YFD0501901"]}]},{"name":"Beijing Innovation Consortium of Livestock Research System","award":["2017YFD0501905"],"award-info":[{"award-number":["2017YFD0501905"]}]},{"name":"Beijing Innovation Consortium of Livestock Research System","award":["31930103"],"award-info":[{"award-number":["31930103"]}]},{"name":"Beijing Innovation Consortium of Livestock Research System","award":["3167246"],"award-info":[{"award-number":["3167246"]}]},{"name":"Beijing Innovation Consortium of Livestock Research System","award":["BAIC05-2022"],"award-info":[{"award-number":["BAIC05-2022"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Genes"],"abstract":"<jats:p>Mammalian preimplantation development depends on the interaction between embryonic autocrine and maternal paracrine signaling. Despite the robust independence of preimplantation embryos, oviductal factors are thought to be critical to pregnancy success. However, how oviductal factors regulate embryonic development and the underlying mechanism remain unknown. In the present study, focusing on WNT signaling, which has been reported to be essential for developmental reprogramming after fertilization, we analyzed the receptor-ligand repertoire of preimplantation embryonic WNT signaling, and identified that the WNT co-receptor LRP6 is necessary for early cleavage and has a prolonged effect on preimplantation development. LRP6 inhibition significantly impeded zygotic genome activation and disrupted relevant epigenetic reprogramming. Focusing on the potential oviductal WNT ligands, we found WNT2 as the candidate interacting with embryonic LRP6. More importantly, we found that WNT2 supplementation in culture medium significantly promoted zygotic genome activation (ZGA) and improved blastocyst formation and quality following in vitro fertilization (IVF). In addition, WNT2 supplementation significantly improved implantation rate and pregnancy outcomes following embryo transfer. Collectively, our findings not only provide novel insight into how maternal factors regulate preimplantation development through maternal-embryonic communication, but they also propose a promising strategy for improving current IVF systems.<\/jats:p>","DOI":"10.3390\/genes14040891","type":"journal-article","created":{"date-parts":[[2023,4,11]],"date-time":"2023-04-11T02:02:33Z","timestamp":1681178553000},"page":"891","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["WNT Co-Receptor LRP6 Is Critical for Zygotic Genome Activation and Embryonic Developmental Potential by Interacting with Oviductal Paracrine Ligand WNT2"],"prefix":"10.3390","volume":"14","author":[{"given":"Fusheng","family":"Yao","sequence":"first","affiliation":[{"name":"State Key Laboratory of Animal Biotech Breeding, National Engineering Laboratory for Animal Breeding, Key Laboratory of Animal Genetics, Breeding and Reproduction of the Ministry of Agriculture and Rural Affairs, College of Animal Science and Technology, China Agricultural University, No. 2 Yuanmingyuan West Road, Beijing 100193, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jia","family":"Hao","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Animal Biotech Breeding, National Engineering Laboratory for Animal Breeding, Key Laboratory of Animal Genetics, Breeding and Reproduction of the Ministry of Agriculture and Rural Affairs, College of Animal Science and Technology, China Agricultural University, No. 2 Yuanmingyuan West Road, Beijing 100193, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhaochen","family":"Wang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Animal Biotech Breeding, National Engineering Laboratory for Animal Breeding, Key Laboratory of Animal Genetics, Breeding and Reproduction of the Ministry of Agriculture and Rural Affairs, College of Animal Science and Technology, China Agricultural University, No. 2 Yuanmingyuan West Road, Beijing 100193, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meiqiang","family":"Chu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Animal Biotech Breeding, National Engineering Laboratory for Animal Breeding, Key Laboratory of Animal Genetics, Breeding and Reproduction of the Ministry of Agriculture and Rural Affairs, College of Animal Science and Technology, China Agricultural University, No. 2 Yuanmingyuan West Road, Beijing 100193, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jingyu","family":"Zhang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Animal Biotech Breeding, National Engineering Laboratory for Animal Breeding, Key Laboratory of Animal Genetics, Breeding and Reproduction of the Ministry of Agriculture and Rural Affairs, College of Animal Science and Technology, China Agricultural University, No. 2 Yuanmingyuan West Road, Beijing 100193, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guangyin","family":"Xi","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Animal Biotech Breeding, National Engineering Laboratory for Animal Breeding, Key Laboratory of Animal Genetics, Breeding and Reproduction of the Ministry of Agriculture and Rural Affairs, College of Animal Science and Technology, China Agricultural University, No. 2 Yuanmingyuan West Road, Beijing 100193, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhenni","family":"Zhang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Animal Biotech Breeding, National Engineering Laboratory for Animal Breeding, Key Laboratory of Animal Genetics, Breeding and Reproduction of the Ministry of Agriculture and Rural Affairs, College of Animal Science and Technology, China Agricultural University, No. 2 Yuanmingyuan West Road, Beijing 100193, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lei","family":"An","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Animal Biotech Breeding, National Engineering Laboratory for Animal Breeding, Key Laboratory of Animal Genetics, Breeding and Reproduction of the Ministry of Agriculture and Rural Affairs, College of Animal Science and Technology, China Agricultural University, No. 2 Yuanmingyuan West Road, Beijing 100193, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianhui","family":"Tian","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Animal Biotech Breeding, National Engineering Laboratory for Animal Breeding, Key Laboratory of Animal Genetics, Breeding and Reproduction of the Ministry of Agriculture and Rural Affairs, College of Animal Science and Technology, China Agricultural University, No. 2 Yuanmingyuan West Road, Beijing 100193, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,4,10]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"221","DOI":"10.1677\/joe.0.1720221","article-title":"Growth factor expression and function in the human and mouse preimplantation embryo","volume":"172","author":"Hardy","year":"2002","journal-title":"J. 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