{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T02:11:46Z","timestamp":1760148706331,"version":"build-2065373602"},"reference-count":49,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2023,5,31]],"date-time":"2023-05-31T00:00:00Z","timestamp":1685491200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31972550","2020B1515420008","2023A1515011057","060302052104"],"award-info":[{"award-number":["31972550","2020B1515420008","2023A1515011057","060302052104"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Natural Science Foundation of Guangdong Province","award":["31972550","2020B1515420008","2023A1515011057","060302052104"],"award-info":[{"award-number":["31972550","2020B1515420008","2023A1515011057","060302052104"]}]},{"name":"Program for Scientific Research Start-up funds of Guangdong Ocean University","award":["31972550","2020B1515420008","2023A1515011057","060302052104"],"award-info":[{"award-number":["31972550","2020B1515420008","2023A1515011057","060302052104"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Genes"],"abstract":"<jats:p>The anterior pituitary gland of animals secretes growth hormone (GH) to bind to the growth hormone receptor (GHR) on the liver cell membrane through the blood circulation, thereby promoting the downstream gene insulin-like growth factor-1 (IGF1) expression, which is the canonical GH\u2013GHR\u2013IGF1 signaling pathway. Therefore, the amount of GHR and the integrity of its structure will affect animal growth and development. In the previous study, we found that the mouse GHR gene can transcribe a circular transcript named circGHR. Our group cloned the full-length of the mouse circGHR and analyzed its spatiotemporal expression profile. In this study, we further predicted the open reading frame of circGHR with bioinformatics, subsequently constructed a Flag-tagged protein vector and preliminarily verified its coding potential with western blot. Additionally, we found that circGHR could inhibit the proliferation of NCTC469 cells and has a tendency to inhibit cell apoptosis, while for C2C12 cells, it showed a tendency to inhibit cell proliferation and promote its differentiation. Overall, these results suggested that the mouse circGHR had the potential to encode proteins and affect cell proliferation, differentiation and apoptosis.<\/jats:p>","DOI":"10.3390\/genes14061207","type":"journal-article","created":{"date-parts":[[2023,6,1]],"date-time":"2023-06-01T01:40:56Z","timestamp":1685583656000},"page":"1207","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["The Mouse CircGHR Regulates Proliferation, Differentiation and Apoptosis of Hepatocytes and Myoblasts"],"prefix":"10.3390","volume":"14","author":[{"given":"Weilu","family":"Zhang","sequence":"first","affiliation":[{"name":"College of Coastal Agricultural Sciences, Guangdong Ocean University, Zhanjiang 524088, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8520-8614","authenticated-orcid":false,"given":"Shudai","family":"Lin","sequence":"additional","affiliation":[{"name":"College of Coastal Agricultural Sciences, Guangdong Ocean University, Zhanjiang 524088, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhenhai","family":"Jiao","sequence":"additional","affiliation":[{"name":"College of Coastal Agricultural Sciences, Guangdong Ocean University, Zhanjiang 524088, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lilong","family":"An","sequence":"additional","affiliation":[{"name":"College of Coastal Agricultural Sciences, Guangdong Ocean University, Zhanjiang 524088, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tingting","family":"Xie","sequence":"additional","affiliation":[{"name":"College of Coastal Agricultural Sciences, Guangdong Ocean University, Zhanjiang 524088, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0771-1791","authenticated-orcid":false,"given":"Jiang","family":"Wu","sequence":"additional","affiliation":[{"name":"Experimental Teaching Center, Guangdong Ocean University, Zhanjiang 524088, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Li","family":"Zhang","sequence":"additional","affiliation":[{"name":"College of Coastal Agricultural Sciences, Guangdong Ocean University, Zhanjiang 524088, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2023,5,31]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"3852","DOI":"10.1073\/pnas.73.11.3852","article-title":"Viroids are single-stranded covalently closed circular RNA molecules existing as highly base-paired rod-like structures","volume":"73","author":"Sanger","year":"1976","journal-title":"Proc. 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