{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T07:26:31Z","timestamp":1792135591418,"version":"4.4.0"},"reference-count":62,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2023,7,14]],"date-time":"2023-07-14T00:00:00Z","timestamp":1689292800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Programa Nacional de Coopera\u00e7\u00e3o Acad\u00eamica na Amaz\u00f4nia","award":["21\/2018"],"award-info":[{"award-number":["21\/2018"]}]},{"name":"Programa Nacional de Coopera\u00e7\u00e3o Acad\u00eamica na Amaz\u00f4nia","award":["88887.761304\/2022-00"],"award-info":[{"award-number":["88887.761304\/2022-00"]}]},{"name":"Programa Nacional de Coopera\u00e7\u00e3o Acad\u00eamica na Amaz\u00f4nia","award":["314522\/2021-2"],"award-info":[{"award-number":["314522\/2021-2"]}]},{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"publisher","award":["21\/2018"],"award-info":[{"award-number":["21\/2018"]}],"id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"publisher","award":["88887.761304\/2022-00"],"award-info":[{"award-number":["88887.761304\/2022-00"]}],"id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"publisher","award":["314522\/2021-2"],"award-info":[{"award-number":["314522\/2021-2"]}],"id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","award":["21\/2018"],"award-info":[{"award-number":["21\/2018"]}]},{"name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","award":["88887.761304\/2022-00"],"award-info":[{"award-number":["88887.761304\/2022-00"]}]},{"name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","award":["314522\/2021-2"],"award-info":[{"award-number":["314522\/2021-2"]}]},{"name":"Evandro Chagas Institute, Stadual University of Maranh\u00e3o","award":["21\/2018"],"award-info":[{"award-number":["21\/2018"]}]},{"name":"Evandro Chagas Institute, Stadual University of Maranh\u00e3o","award":["88887.761304\/2022-00"],"award-info":[{"award-number":["88887.761304\/2022-00"]}]},{"name":"Evandro Chagas Institute, Stadual University of Maranh\u00e3o","award":["314522\/2021-2"],"award-info":[{"award-number":["314522\/2021-2"]}]},{"name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa e Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico do Maranh\u00e3o","award":["21\/2018"],"award-info":[{"award-number":["21\/2018"]}]},{"name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa e Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico do Maranh\u00e3o","award":["88887.761304\/2022-00"],"award-info":[{"award-number":["88887.761304\/2022-00"]}]},{"name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa e Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico do Maranh\u00e3o","award":["314522\/2021-2"],"award-info":[{"award-number":["314522\/2021-2"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Genes"],"abstract":"<jats:p>A wide diversity of pathogenic mosquito-borne viruses circulate in the Brazilian Amazon, and the intense deforestation can contribute to the spread of these viruses. In this context, this study aimed to investigate the viral diversity in mosquitoes of the genera Aedes, Culex, Haemagogus, and Sabethes from a transition area between the Amazon, Cerrado, and Caatinga biomes in Brazil. Metagenomic high-throughput sequencing was used to characterize the virome of 20 mosquito pools. A total of 15 virus-like genomes were identified, comprising species genomically close to insect-specific viruses of the families Iflaviridae, Metaviridae, Lispiviridae, Rhabdoviridae, Xinmoviridae, and Parvoviridae and species of plant viruses of the families Solemoviridae, Virgaviridae, and Partitiviridae. However, sequences of viruses associated with human and animal diseases were not detected. Most of the recovered genomes were divergent from those previously described. These findings reveal that there are a large number of unknown viruses to be explored in the middle-north of Brazil.<\/jats:p>","DOI":"10.3390\/genes14071443","type":"journal-article","created":{"date-parts":[[2023,7,14]],"date-time":"2023-07-14T00:28:06Z","timestamp":1689294486000},"page":"1443","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":23,"title":["Shotgun Metagenomic Sequencing Reveals Virome Composition of Mosquitoes from a Transition Ecosystem of North-Northeast Brazil"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2721-6496","authenticated-orcid":false,"given":"Carine Fortes","family":"Arag\u00e3o","sequence":"first","affiliation":[{"name":"Department of Arbovirology and Hemorrhagic Fevers, Evandro Chagas Institute, Secretariat of Health and Environment Surveillance, Ministry of Health, Ananindeua 67030-000, PA, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sandro Patroca","family":"da Silva","sequence":"additional","affiliation":[{"name":"Department of Arbovirology and Hemorrhagic Fevers, Evandro Chagas Institute, Secretariat of Health and Environment Surveillance, Ministry of Health, Ananindeua 67030-000, PA, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8529-4751","authenticated-orcid":false,"given":"Bruna La\u00eds Sena","family":"do Nascimento","sequence":"additional","affiliation":[{"name":"Department of Arbovirology and Hemorrhagic Fevers, Evandro Chagas Institute, Secretariat of Health and Environment Surveillance, Ministry of Health, Ananindeua 67030-000, PA, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"F\u00e1bio Silva","family":"da Silva","sequence":"additional","affiliation":[{"name":"Department of Arbovirology and Hemorrhagic Fevers, Evandro Chagas Institute, Secretariat of Health and Environment Surveillance, Ministry of Health, Ananindeua 67030-000, PA, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5981-0065","authenticated-orcid":false,"given":"Joaquim Pinto","family":"Nunes Neto","sequence":"additional","affiliation":[{"name":"Department of Arbovirology and Hemorrhagic Fevers, Evandro Chagas Institute, Secretariat of Health and Environment Surveillance, Ministry of Health, Ananindeua 67030-000, PA, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Val\u00e9ria Cristina Soares","family":"Pinheiro","sequence":"additional","affiliation":[{"name":"Laboratory of Medical Entomology, Stadual University of Maranh\u00e3o, Caxias 65604-380, MA, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4607-9346","authenticated-orcid":false,"given":"Ana Cec\u00edlia Ribeiro","family":"Cruz","sequence":"additional","affiliation":[{"name":"Department of Arbovirology and Hemorrhagic Fevers, Evandro Chagas Institute, Secretariat of Health and Environment Surveillance, Ministry of Health, Ananindeua 67030-000, PA, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,7,14]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"26","DOI":"10.1016\/j.coviro.2019.05.005","article-title":"Curious Entanglements: Interactions between Mosquitoes, Their Microbiota, and Arboviruses","volume":"37","author":"Caragata","year":"2019","journal-title":"Curr. Opin. Virol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"50","DOI":"10.1016\/j.cois.2020.02.005","article-title":"Exploiting Insect-Specific Viruses as a Novel Strategy to Control Vector-Borne Disease","volume":"39","author":"Patterson","year":"2020","journal-title":"Curr. Opin. Insect Sci."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"4911","DOI":"10.3390\/v7092851","article-title":"Insect-Specific Virus Discovery: Significance for the Arbovirus Community","volume":"7","author":"Bolling","year":"2015","journal-title":"Viruses"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1016\/j.coviro.2018.07.006","article-title":"Insect-Specific Viruses: From Discovery to Potential Translational Applications","volume":"33","author":"Nouri","year":"2018","journal-title":"Curr. Opin. Virol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"433","DOI":"10.1128\/JVI.00433-21","article-title":"Negeviruses Reduce Replication of Alphaviruses during Coinfection VIRUS-CELL INTERACTIONS","volume":"95","author":"Patterson","year":"2021","journal-title":"J. Virol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"e00705-19","DOI":"10.1128\/JVI.00705-19","article-title":"Cell-Fusing Agent Virus Reduces Arbovirus Dissemination in Aedes Aegypti Mosquitoes In Vivo","volume":"93","author":"Baidaliuk","year":"2019","journal-title":"J. Virol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1038\/s41426-018-0180-4","article-title":"Restriction of Zika Virus Infection and Transmission in Aedes Aegypti Mediated by an Insect-Specific Flavivirus","volume":"7","author":"Romo","year":"2018","journal-title":"Emerg. Microbes Infect."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"127","DOI":"10.1007\/s11262-018-01629-9","article-title":"Insect-Specific Virus Evolution and Potential Effects on Vector Competence","volume":"55","year":"2019","journal-title":"Virus Genes"},{"key":"ref_9","unstructured":"Le\u00e3o, R.N.Q., Bichara, C.N.C., Fraiha Neto, H., and Vasconcelos, P.F.d.C. (2013). Medicina Tropical e Infectologia na Amaz\u00f4nia, Samauma Editorial."},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"Ramos, B.A., Das Chagas, L.L., de Arruda e Silva, F., dos Santos, E.B., Chiang, J.O., Neto, J.P.N., Vieira, D.B.R., Junior, J.W.R., da Silva, E.V.P., and Freitas, M.N.O. (2022). Arboviruses in Free-Ranging Birds and Hematophagous Arthropods (Diptera, Nematocera) from Forest Remnants and Urbanized Areas of an Environmental Protection Area in the Amazon Biome. Viruses, 14.","DOI":"10.3390\/v14102101"},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Ara\u00fajo, P.A., Freitas, M.O., Chiang, J.O., Silva, F.A., Chagas, L.L., Casseb, S.M., Silva, S.P., Nunes-Neto, J.P., Rosa-J\u00fanior, J.W., and Nascimento, B.S. (2019). Investigation about the Occurrence of Transmission Cycles of Arbovirus in the Tropical Forest, Amazon Region. Viruses, 11.","DOI":"10.3390\/v11090774"},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Silva, F.A., Ferreira, M.S., Ara\u00fajo, P.A., Casseb, S.M.M., Silva, S.P., Nunes Neto, J.P., Chiang, J.O., Rosa Junior, J.W., Chagas, L.L., and Freitas, M.N.O. (2022). Serological and Molecular Evidence of the Circulation of the Venezuelan Equine Encephalitis Virus Subtype IIIA in Humans, Wild Vertebrates and Mosquitos in the Brazilian Amazon. Viruses, 14.","DOI":"10.3390\/v14112391"},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Nunes-Neto, J.P., Reis, L.A.M., Freitas, M.N.O., Nascimento, B.L.S., Chagas, L.L., Costa, H.H.M., Rodrigues, J.C.P., Braga, C.M., Silva, E.V.P., and Silva, S.P. (2023). First Isolation and Genome Sequence Analysis of West Nile Virus in Mosquitoes in Brazil. Trop. Med. Infect. Dis., 8.","DOI":"10.3390\/tropicalmed8040237"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"m4385","DOI":"10.1136\/bmj.m4385","article-title":"Emerging Arboviruses in the Urbanized Amazon Rainforest","volume":"371","author":"Lowe","year":"2020","journal-title":"BMJ"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"473","DOI":"10.1016\/j.pt.2020.02.003","article-title":"Not Just from Blood: Mosquito Nutrient Acquisition from Nectar Sources","volume":"36","author":"Barredo","year":"2020","journal-title":"Trends Parasitol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1016\/j.cois.2019.02.002","article-title":"Mosquito Adaptations to Hematophagia Impact Pathogen Transmission","volume":"34","author":"Nouzova","year":"2019","journal-title":"Curr. Opin. Insect Sci."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"1","DOI":"10.21775\/cimb.034.001","article-title":"The Insect Virome: Opportunities and Challenges","volume":"34","author":"Bonning","year":"2020","journal-title":"Curr. Issues Mol. Biol."},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"He, X., Yin, Q., Zhou, L., Meng, L., Hu, W., Li, F., Li, Y., Han, K., Zhang, S., and Fu, S. (2021). Metagenomic Sequencing Reveals Viral Abundance and Diversity in Mosquitoes from the Shaanxi-Gansu-Ningxia Region, China. PLoS Negl. Trop. Dis., 15.","DOI":"10.1371\/journal.pntd.0009381"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"116","DOI":"10.1016\/j.apgeog.2015.01.017","article-title":"Land Cover Changes in the Brazilian Cerrado and Caatinga Biomes from 1990 to 2010 Based on a Systematic Remote Sensing Sampling Approach","volume":"58","author":"Beuchle","year":"2015","journal-title":"Appl. Geogr."},{"key":"ref_20","unstructured":"Lane, J. (1953). Neotropical Culicidae, Edusp."},{"key":"ref_21","unstructured":"Lane, J. (1953). Neotropical Culicidae, Edusp."},{"key":"ref_22","unstructured":"Forattini, O.P. (1965). Entomologia M\u00e9dica. Culicini: Culex, Aedes e Psorophora, Editora da Universidade de S\u00e3o Paulo."},{"key":"ref_23","unstructured":"Forattini, O.P. (1962). Entomologia M\u00e9dica. Parte Geral, Diptera, Anophelini, Faculdade de Higiene e Sa\u00fade P\u00fablica."},{"key":"ref_24","unstructured":"Forattini, O.P. (1965). Entomologia M\u00e9dica. Culicini: Haemagogus, Mansonia, Culiseta, Sabethini, Toxorhynchitini, Arboviroses, Filariose Bancroftiana, Gen\u00e9tica, Editora da Universidade de S\u00e3o Paulo."},{"key":"ref_25","unstructured":"Forattini, O.P. (2002). Culicidologia M\u00e9dica, Universidade de S\u00e3o Paulo."},{"key":"ref_26","doi-asserted-by":"crossref","unstructured":"Consoli, R.A.G.B., and Louren\u00e7o-de-Oliveira, R. (1994). Principais Mosquitos de Import\u00e2ncia Sanit\u00e1ria No Brasil, Editora Fiocruz.","DOI":"10.7476\/9788575412909"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"1053","DOI":"10.4269\/ajtmh.1979.28.1053","article-title":"A Method for the Isolation and Identification of Dengue Viruses, Using Mosquito Cell Cultures","volume":"28","author":"Tesh","year":"1979","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"i884","DOI":"10.1093\/bioinformatics\/bty560","article-title":"Fastp: An Ultra-Fast All-in-One FASTQ Preprocessor","volume":"34","author":"Chen","year":"2018","journal-title":"Bioinformatics"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1038\/nmeth.3176","article-title":"Fast and Sensitive Protein Alignment Using DIAMOND","volume":"12","author":"Buchfink","year":"2015","journal-title":"Nat. Methods"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"377","DOI":"10.1101\/gr.5969107","article-title":"MEGAN Analysis of Metagenomic Data","volume":"17","author":"Huson","year":"2007","journal-title":"Genome Res."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"90","DOI":"10.1016\/S0022-2496(02)00028-7","article-title":"Tutorial on Maximum Likelihood Estimation","volume":"47","author":"Myung","year":"2003","journal-title":"J. Math. Psychol."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"783","DOI":"10.2307\/2408678","article-title":"Confidence Limits on Phylogenies: An Approach Using the Bootstrap","volume":"39","author":"Felsenstein","year":"1985","journal-title":"Evolution"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"527","DOI":"10.1099\/jgv.0.000757","article-title":"ICTV Virus Taxonomy Profile: Iflaviridae","volume":"98","author":"Valles","year":"2017","journal-title":"J. Gen. Virol."},{"key":"ref_34","first-page":"001707","article-title":"ICTV Virus Taxonomy Profile: Solemoviridae 2021","volume":"102","author":"Fargette","year":"2021","journal-title":"J. Gen. Virol."},{"key":"ref_35","unstructured":"Chen, Y., Valles, S., Firth, A., Miranda, J., Ryabov, E., Echeverr\u00eda, M., Schroeder, D., Jan, E., Zheng, H., and Parry, R. (2022, December 19). Family: Iflaviridae. Available online: https:\/\/ictv.global\/report\/chapter\/iflaviridae\/iflaviridae."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"406","DOI":"10.1016\/j.virol.2018.03.022","article-title":"Dual Insect Specific Virus Infection Limits Arbovirus Replication in Aedes Mosquito Cells","volume":"518","author":"Schultz","year":"2018","journal-title":"Virology"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"3362","DOI":"10.1002\/jmv.26686","article-title":"Mosquito-Infecting Virus Espirito Santo Virus Inhibits Replication and Spread of Dengue Virus","volume":"93","author":"White","year":"2021","journal-title":"J. Med. Virol."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"210","DOI":"10.1186\/s13071-020-04072-8","article-title":"Anopheles Gambiae Densovirus (AgDNV) Negatively Affects Mayaro Virus Infection in Anopheles Gambiae Cells and Mosquitoes","volume":"13","author":"Urakova","year":"2020","journal-title":"Parasit. Vectors"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"198703","DOI":"10.1016\/j.virusres.2022.198703","article-title":"Genetic Characterisation of an Iflavirus Associated with a Vomiting Disease in the Indian Tropical Tasar Silkworm, Antheraea Mylitta","volume":"311","author":"Ponnuvel","year":"2022","journal-title":"Virus Res."},{"key":"ref_40","doi-asserted-by":"crossref","unstructured":"Carballo, A., Williams, T., Murillo, R., and Caballero, P. (2020). Iflavirus Covert Infection Increases Susceptibility to Nucleopolyhedrovirus Disease in Spodoptera Exigua. Viruses, 12.","DOI":"10.3390\/v12050509"},{"key":"ref_41","doi-asserted-by":"crossref","unstructured":"Linscheid, Y., Kessel, T., Vilcinskas, A., and Lee, K.Z. (2022). Pathogenicity of La Jolla Virus in Drosophila Suzukii Following Oral Administration. Viruses, 14.","DOI":"10.3390\/v14102158"},{"key":"ref_42","doi-asserted-by":"crossref","unstructured":"Faizah, A.N., Kobayashi, D., Isawa, H., Amoa-Bosompem, M., Murota, K., Higa, Y., Futami, K., Shimada, S., Kim, K.S., and Itokawa, K. (2020). Deciphering the Virome of Culex Vishnui Subgroup Mosquitoes, the Major Vectors of Japanese Encephalitis, in Japan. Viruses, 12.","DOI":"10.3390\/v12030264"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1186\/s12985-018-0981-z","article-title":"Genetic Characterization of a Novel Picorna-like Virus in Culex spp. Mosquitoes from Mozambique","volume":"15","author":"Cholleti","year":"2018","journal-title":"Virol. J."},{"key":"ref_44","doi-asserted-by":"crossref","unstructured":"Atoni, E., Wang, Y., Karungu, S., Waruhiu, C., Zohaib, A., Obanda, V., Agwanda, B., Mutua, M., Xia, H., and Yuan, Z. (2018). Metagenomic Virome Analysis of Culex Mosquitoes from Kenya and China. Viruses, 10.","DOI":"10.3390\/v10010030"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"e68353","DOI":"10.7554\/eLife.68353","article-title":"Single Mosquito Metatranscriptomics Identifies Vectors, Emerging Pathogens and Reservoirs in One Assay","volume":"10","author":"Batson","year":"2021","journal-title":"eLife"},{"key":"ref_46","doi-asserted-by":"crossref","unstructured":"de Oliveira Ribeiro, G., Morais, V.S., Monteiro, F.J.C., Ribeiro, E.S.D.A., da Rego, M.O.S., Souto, R.N.P., Villanova, F., Tahmasebi, R., Hefford, P.M., and Deng, X. (2020). Aedes Aegypti from Amazon Basin Harbor High Diversity of Novel Viral Species. Viruses, 12.","DOI":"10.3390\/v12080866"},{"key":"ref_47","doi-asserted-by":"crossref","unstructured":"Parry, R., Naccache, F., Ndiaye, E.H., Fall, G., Castelli, I., L\u00fchken, R., Medlock, J., Cull, B., Hesson, J.C., and Montarsi, F. (2020). Identification and RNAi Profile of a Novel Iflavirus Infecting Senegalese Aedes Vexans Arabiensis Mosquitoes. Viruses, 12.","DOI":"10.3390\/v12040440"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"36","DOI":"10.4161\/mge.19234","article-title":"Drosophila Errantiviruses","volume":"2","author":"Stefanov","year":"2012","journal-title":"Mob. Genet. Elem."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"1131","DOI":"10.1099\/jgv.0.001509","article-title":"ICTV Virus Taxonomy Profile: Metaviridae","volume":"101","author":"Llorens","year":"2020","journal-title":"J. Gen. Virol."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1007\/s42770-021-00632-x","article-title":"Identification of Potential New Mosquito-Associated Viruses of Adult Aedes Aegypti Mosquitoes from Tocantins State, Brazil","volume":"53","author":"Duarte","year":"2022","journal-title":"Braz. J. Microbiol."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"367","DOI":"10.1099\/jgv.0.001212","article-title":"ICTV Virus Taxonomy Profile: Parvoviridae","volume":"100","author":"Cotmore","year":"2019","journal-title":"J. Gen. Virol."},{"key":"ref_52","unstructured":"(2023, May 07). ICTV Subfamily: Densovirinae. Available online: https:\/\/ictv.global\/report\/chapter\/parvoviridae\/parvoviridae\/densovirinae."},{"key":"ref_53","doi-asserted-by":"crossref","unstructured":"Caicedo, E.Y., Charniga, K., Rueda, A., Dorigatti, I., Mendez, Y., Hamlet, A., Carrera, J.P., and Cucunub\u00e1, Z.M. (2021). The Epidemiology of Mayaro Virus in the Americas: A Systematic Review and Key Parameter Estimates for Outbreak Modelling. PLoS Negl. Trop. Dis., 15.","DOI":"10.1371\/journal.pntd.0009418"},{"key":"ref_54","first-page":"883","article-title":"Rhabdoviruses of Insects (Rhabdoviridae)","volume":"4","author":"Rosenbaum","year":"2021","journal-title":"Encycl. Virol."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"e05378","DOI":"10.7554\/eLife.05378","article-title":"Unprecedented Genomic Diversity of RNA Viruses in Arthropods Reveals the Ancestry of Negative-Sense RNA Viruses","volume":"4","author":"Li","year":"2015","journal-title":"eLife"},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"659","DOI":"10.1128\/JVI.02036-15","article-title":"Divergent Viruses Discovered in Arthropods and Vertebrates Revise the Evolutionary History of the Flaviviridae and Related Viruses","volume":"90","author":"Shi","year":"2016","journal-title":"J. Virol."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"1233","DOI":"10.1007\/s00705-018-04126-4","article-title":"Taxonomy of the Order Mononegavirales: Second Update 2018","volume":"164","author":"Maes","year":"2019","journal-title":"Arch. Virol."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"10-1128","DOI":"10.1128\/JVI.00224-18","article-title":"Aedes Anphevirus: An Insect-Specific Virus Distributed Worldwide in Aedes Aegypti Mosquitoes That Has Complex Interplays with Wolbachia and Dengue Virus Infection in Cells","volume":"92","author":"Parry","year":"2018","journal-title":"J. Virol."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"104561","DOI":"10.1016\/j.meegid.2020.104561","article-title":"Insect-Specific Viruses and Arboviruses in Adult Male Culicids from Midwestern Brazil","volume":"85","author":"Souza","year":"2020","journal-title":"Infect. Genet. Evol."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"18","DOI":"10.1094\/MPMI-07-19-0184-CR","article-title":"Cell Biology during Infection of Plant Viruses in Insect Vectors and Plant Hosts","volume":"33","author":"Chen","year":"2020","journal-title":"Mol. Plant-Microbe Interact."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1099\/jgv.0.000985","article-title":"ICTV Virus Taxonomy Profile: Partitiviridae","volume":"99","author":"Vainio","year":"2018","journal-title":"J. Gen. Virol."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"161","DOI":"10.1038\/nrmicro.2016.177","article-title":"Virus Taxonomy in the Age of Metagenomics","volume":"15","author":"Simmonds","year":"2017","journal-title":"Nat. Rev. Microbiol."}],"container-title":["Genes"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2073-4425\/14\/7\/1443\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T20:11:43Z","timestamp":1760127103000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2073-4425\/14\/7\/1443"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,7,14]]},"references-count":62,"journal-issue":{"issue":"7","published-online":{"date-parts":[[2023,7]]}},"alternative-id":["genes14071443"],"URL":"https:\/\/doi.org\/10.3390\/genes14071443","relation":{},"ISSN":["2073-4425"],"issn-type":[{"value":"2073-4425","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,7,14]]}}}